EP1448580A4 - Durch rna-interferenz vermittelte inhibierung der genexpression von proliferating cell nuclear antigen (pcna) und verwendung von short interfering nucleic acid (sina) - Google Patents

Durch rna-interferenz vermittelte inhibierung der genexpression von proliferating cell nuclear antigen (pcna) und verwendung von short interfering nucleic acid (sina)

Info

Publication number: EP1448580A4
Authority: EP; European Patent Office
Prior art keywords: interference; sina; pcna; nucleic acid; gene expression
Prior art date: 2002-02-20
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Withdrawn

Application number

EP03716055A

Other languages

English (en)

French (fr)

Other versions

EP1448580A2 (de

Inventor

James Mcswiggen

Bharat Chowrira

Leonid Beigelman

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Sirna Therapeutics Inc

Original Assignee

Sirna Therapeutics Inc

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

2002-02-20

Filing date

2003-02-18

Publication date

2005-07-20

2003-02-18 Application filed by Sirna Therapeutics Inc filed Critical Sirna Therapeutics Inc

2004-08-25 Publication of EP1448580A2 publication Critical patent/EP1448580A2/de

2005-07-20 Publication of EP1448580A4 publication Critical patent/EP1448580A4/de

Status Withdrawn legal-status Critical Current

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/11—Antisense
- C12N2310/111—Antisense spanning the whole gene, or a large part of it
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/14—Type of nucleic acid interfering nucleic acids [NA]
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/30—Chemical structure
- C12N2310/31—Chemical structure of the backbone
- C12N2310/315—Phosphorothioates
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/30—Chemical structure
- C12N2310/31—Chemical structure of the backbone
- C12N2310/317—Chemical structure of the backbone with an inverted bond, e.g. a cap structure
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/30—Chemical structure
- C12N2310/31—Chemical structure of the backbone
- C12N2310/318—Chemical structure of the backbone where the PO2 is completely replaced, e.g. MMI or formacetal
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/30—Chemical structure
- C12N2310/32—Chemical structure of the sugar
- C12N2310/321—2'-O-R Modification
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/30—Chemical structure
- C12N2310/32—Chemical structure of the sugar
- C12N2310/322—2'-R Modification
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/30—Chemical structure
- C12N2310/33—Chemical structure of the base
- C12N2310/332—Abasic residue
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/50—Physical structure
- C12N2310/53—Physical structure partially self-complementary or closed

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Health & Medical Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Engineering & Computer Science (AREA)
Genetics & Genomics (AREA)
Biomedical Technology (AREA)
Chemical & Material Sciences (AREA)
Molecular Biology (AREA)
Organic Chemistry (AREA)
Biotechnology (AREA)
General Engineering & Computer Science (AREA)
Zoology (AREA)
Bioinformatics & Cheminformatics (AREA)
Wood Science & Technology (AREA)
Microbiology (AREA)
Plant Pathology (AREA)
Physics & Mathematics (AREA)
Biochemistry (AREA)
General Health & Medical Sciences (AREA)
Biophysics (AREA)
Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

EP03716055A 2002-02-20 2003-02-18 Durch rna-interferenz vermittelte inhibierung der genexpression von proliferating cell nuclear antigen (pcna) und verwendung von short interfering nucleic acid (sina) Withdrawn EP1448580A4 (de)

Applications Claiming Priority (17)

Application Number	Priority Date	Filing Date	Title
US35858002P	2002-02-20	2002-02-20
US358580P		2002-02-20
US36312402P	2002-03-11	2002-03-11
US363124P		2002-03-11
US38678202P	2002-06-06	2002-06-06
US386782P		2002-06-06
US40678402P	2002-08-29	2002-08-29
US406784P		2002-08-29
US40837802P	2002-09-05	2002-09-05
US408378P		2002-09-05
US40929302P	2002-09-09	2002-09-09
US409293P		2002-09-09
US40978502P	2002-09-11	2002-09-11
US44012903P	2003-01-15	2003-01-15
US440129P		2003-01-15
PCT/US2003/004738 WO2003070896A2 (en)	2002-02-20	2003-02-18	RNA INTERFERENCE MEDIATED INHIBITION OF PROLIFERATING CELL NUCLEAR ANTIGEN (PCNA) GENE EXPRESSION USING SHORT INTERFERING NUCLEIC ACID (siNA)
US409785P		2010-11-03

Publications (2)

Publication Number	Publication Date
EP1448580A2 EP1448580A2 (de)	2004-08-25
EP1448580A4 true EP1448580A4 (de)	2005-07-20

Family

ID=27761719

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
EP03716055A Withdrawn EP1448580A4 (de)	2002-02-20	2003-02-18	Durch rna-interferenz vermittelte inhibierung der genexpression von proliferating cell nuclear antigen (pcna) und verwendung von short interfering nucleic acid (sina)

Country Status (3)

Country	Link
EP (1)	EP1448580A4 (de)
AU (1)	AU2003219781A1 (de)
WO (1)	WO2003070896A2 (de)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US9994853B2 (en)	2001-05-18	2018-06-12	Sirna Therapeutics, Inc.	Chemically modified multifunctional short interfering nucleic acid molecules that mediate RNA interference
US20050148530A1 (en)	2002-02-20	2005-07-07	Sirna Therapeutics, Inc.	RNA interference mediated inhibition of vascular endothelial growth factor and vascular endothelial growth factor receptor gene expression using short interfering nucleic acid (siNA)
US7517864B2 (en)	2001-05-18	2009-04-14	Sirna Therapeutics, Inc.	RNA interference mediated inhibition of vascular endothelial growth factor and vascular endothelial growth factor receptor gene expression using short interfering nucleic acid (siNA)
US9181551B2 (en)	2002-02-20	2015-11-10	Sirna Therapeutics, Inc.	RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid (siNA)
US9657294B2 (en)	2002-02-20	2017-05-23	Sirna Therapeutics, Inc.	RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid (siNA)
CA2542835A1 (en) *	2003-10-23	2005-05-19	Sirna Therapeutics, Inc.	Rna interference mediated inhibition of gene expression using short interfering nucleic acid (sina)
ATE452188T1 (de)	2004-02-10	2010-01-15	Sirna Therapeutics Inc	Rna-interferenz-vermittelte hemmung der genexpression unter verwendung multifunktioneller sina (short interfering nucleic acid)
US10508277B2 (en)	2004-05-24	2019-12-17	Sirna Therapeutics, Inc.	Chemically modified multifunctional short interfering nucleic acid molecules that mediate RNA interference
WO2008109372A2 (en) *	2007-03-02	2008-09-12	Mdrna, Inc.	Nucleic acid compounds for inhibiting pdgf gene expression and uses thereof
EP2548027A2 (de) *	2010-03-18	2013-01-23	Institut National de la Santé et de la Recherche Médicale	Verfahren zur vorhersage der ansprechbarkeit auf eine chemotherapie
EP3372684B1 (de)	2010-08-24	2020-10-07	Sirna Therapeutics, Inc.	Einsträngige rnai-mittel mit einem internen nukleinsäurefreien spacer
DK2632472T3 (en)	2010-10-29	2018-03-19	Sirna Therapeutics Inc	RNA INTERFERENCE-MEDIATED INHIBITION OF GENE EXPRESSION USING SHORT INTERFERRING NUCLEIC ACIDS (SINA)

Citations (1)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
WO2001083740A2 (en) *	2000-05-04	2001-11-08	Avi Biopharma, Inc.	Splice-region antisense composition and method

2003
- 2003-02-18 EP EP03716055A patent/EP1448580A4/de not_active Withdrawn
- 2003-02-18 WO PCT/US2003/004738 patent/WO2003070896A2/en not_active Ceased
- 2003-02-18 AU AU2003219781A patent/AU2003219781A1/en not_active Abandoned

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
WO2001083740A2 (en) *	2000-05-04	2001-11-08	Avi Biopharma, Inc.	Splice-region antisense composition and method

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
BASS B L: "RNA interference: the short answer", NATURE, MACMILLAN JOURNALS LTD. LONDON, GB, vol. 411, 24 May 2001 (2001-05-24), pages 428 - 429, XP002239989, ISSN: 0028-0836 *
ELBASHIR SAYDA M ET AL: "RNA interference is mediated by 21- and 22-nucleotide RNAs", GENES AND DEVELOPMENT, COLD SPRING HARBOR LABORATORY PRESS, NEW YORK, US, vol. 15, no. 2, 15 January 2001 (2001-01-15), pages 188 - 200, XP002204651, ISSN: 0890-9369 *
JASKULSKI D ET AL: "INHIBITION OF CELLULAR PROLIFERATION BY ANTISENSE OLIGODEOXYNUCLEOTIDES TO PCNA CYCLIN", SCIENCE, AMERICAN ASSOCIATION FOR THE ADVANCEMENT OF SCIENCE, US, vol. 240, 10 June 1988 (1988-06-10), pages 1544 - 1546, XP002039132, ISSN: 0036-8075 *

Also Published As

Publication number	Publication date
EP1448580A2 (de)	2004-08-25
WO2003070896A2 (en)	2003-08-28
WO2003070896A3 (en)	2004-03-04
AU2003219781A8 (en)	2003-09-09
AU2003219781A1 (en)	2003-09-09

Publication	Publication Date	Title
EP1448580A4 (de)	2005-07-20	Durch rna-interferenz vermittelte inhibierung der genexpression von proliferating cell nuclear antigen (pcna) und verwendung von short interfering nucleic acid (sina)
CY2016020I2 (el)	2016-12-14	Στελεχη ιου του ερπη για γονιδιακη θepαπεια
AU2003211058A8 (en)	2003-09-09	Rna interference mediated target discovery and target validation using short interfering nucleic acid (sina)
IL124967A (en)	2000-07-26	Method for nucleic acid analysis using fluorescence resonance energy transfer
AU2003210895A8 (en)	2003-09-09	Rna interference mediated inhibition of cyclin d1 gene expression using short interfering nucleic acid (sina)
DE60329220D1 (de)	2009-10-22	Antisense-entwurf
WO2005084367A3 (en)	2005-12-22	Photocleavable fluorescent nucleotides for dna sequencing on chip constructed by site-specific coupling chemistry
DE69834056D1 (de)	2006-05-18	De novo oder "universaler" sequenzierungs-array
DE69838449D1 (de)	2007-10-31	Aminooxy-modifizierte oligonukleotide
AU2001283056A1 (en)	2002-02-13	A rapid, quantitative method for the mass spectrometric analysis of nucleic acids for gene expression and genotyping
AU2496900A (en)	2000-07-24	Enhanced sequencing by hybridization using pools of probes
MX9400837A (es)	1994-08-31	Metodos y aparatos para la determinacion de secuencia de adn.
ATE239090T1 (de)	2003-05-15	Methode zum generieren von einzelsträngigen dna molekülen
DE69420454D1 (de)	1999-10-14	Interne Kontrollen für isotherme Nukleinsäure-Amplifizierungsreaktionen
BR9305795A (pt)	1997-02-18	Transferéncia de genes em aves por introdução de dna em musculo in ovo
AU2002347981A1 (en)	2003-05-19	Universal nucleotides for nucleic acid analysis
EP0870832A4 (de)	2001-08-22	Neue dna polymerase
HU9800329D0 (en)	1998-04-28	Nucleic acid construct for the cell cycle regulated expression of structural genes
EP1292329A4 (de)	2005-11-16	Manipulation minussträngiger rna viren durch neuanordnung deren gene und ihre verwendungen(08.01.03)
FI852625A7 (fi)	1986-01-06	Menetelmä kaksisäikeisten nukleiinihappomolekyylien muodostamiseksi yksisäikeisistä nukleiinihappomolekyyleistä.
GB2409457B (en)	2006-05-31	Method to generate or determine nucleic acid tags corresponding to the terminal ends of DNA molecules using sequence analysis of gene expression(terminal SAGE
GB2406568B (en)	2005-09-28	RNA interference mediated inhibition of gene expression using chemically modified short interfering nucleic acid (siNA)
EP1463842A4 (de)	2006-01-25	Rna-interferenz-vermittelte hemmung der expression des gens für proteinkinase c-alpha (pkc-alpha) unter verwendung von sina (short interfering nucleic acid)
EP1499628A4 (de)	2005-06-15	Rna-interferenz-vermittelte hemmung der expression des stearoyl-coa-desaturase (scd)-gens unter verwendung von sina (short interfering nucleic acid)
EP1499631A4 (de)	2006-01-25	Rna-interferenz-vermittelte hemmung von tgf-beta und tgf-beta-rezeptor-genexpression mit kurzinterferenz-nukleinsäure (sina)

Legal Events

Date	Code	Title	Description
2004-07-10	PUAI	Public reference made under article 153(3) epc to a published international application that has entered the european phase	Free format text: ORIGINAL CODE: 0009012
2004-08-25	17P	Request for examination filed	Effective date: 20040429
2004-08-25	AK	Designated contracting states	Kind code of ref document: A2 Designated state(s): AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LI LU MC NL PT SE SI SK TR
2004-08-25	AX	Request for extension of the european patent	Extension state: AL LT LV MK RO
2005-07-20	A4	Supplementary search report drawn up and despatched	Effective date: 20050603
2005-07-20	RIC1	Information provided on ipc code assigned before grant	Ipc: 7C 07H 21/04 B Ipc: 7C 07H 21/02 B Ipc: 7C 12N 15/63 B Ipc: 7C 12P 19/34 B Ipc: 7C 12Q 1/68 A
2009-01-23	STAA	Information on the status of an ep patent application or granted ep patent	Free format text: STATUS: THE APPLICATION IS DEEMED TO BE WITHDRAWN
2009-02-25	18D	Application deemed to be withdrawn	Effective date: 20080902