EP1438567A4 - Methods and systems for microfluidic processing - Google Patents

Methods and systems for microfluidic processing

Info

Publication number: EP1438567A4
Authority: EP; European Patent Office
Prior art keywords: systems; methods; microfluidic processing; microfluidic; processing
Prior art date: 2001-07-26
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Granted

Application number

EP02715213A

Other languages

German (de)

French (fr)

Other versions

EP1438567B1 (en

EP1438567A1 (en

Inventor

Gene Parunak

Kalyan Handique

Betty Wu

Karthik Ganesan

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

HandyLab Inc

Original Assignee

HandyLab Inc

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

2001-07-26

Filing date

2002-03-27

Publication date

2010-06-02

2001-12-14 Priority claimed from US10/014,519 external-priority patent/US7192557B2/en

2001-12-14 Priority claimed from US10/014,520 external-priority patent/US7270786B2/en

2002-02-15 Priority claimed from US10/075,371 external-priority patent/US7323140B2/en

2002-03-27 Application filed by HandyLab Inc filed Critical HandyLab Inc

2002-03-27 Priority to EP18185265.8A priority Critical patent/EP3427834A1/en

2004-07-21 Publication of EP1438567A1 publication Critical patent/EP1438567A1/en

2010-06-02 Publication of EP1438567A4 publication Critical patent/EP1438567A4/en

2018-07-25 Application granted granted Critical

2018-07-25 Publication of EP1438567B1 publication Critical patent/EP1438567B1/en

2022-03-27 Anticipated expiration legal-status Critical

Status Expired - Lifetime legal-status Critical Current

Classifications

- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502738—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by integrated valves
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/0673—Handling of plugs of fluid surrounded by immiscible fluid
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/06—Auxiliary integrated devices, integrated components
- B01L2300/0627—Sensor or part of a sensor is integrated
- B01L2300/0636—Integrated biosensor, microarrays
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0809—Geometry, shape and general structure rectangular shaped
- B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
- B01L2400/0442—Moving fluids with specific forces or mechanical means specific forces thermal energy, e.g. vaporisation, bubble jet

Landscapes

Chemical & Material Sciences (AREA)
Health & Medical Sciences (AREA)
Clinical Laboratory Science (AREA)
Chemical Kinetics & Catalysis (AREA)
General Health & Medical Sciences (AREA)
Analytical Chemistry (AREA)
Hematology (AREA)
Dispersion Chemistry (AREA)
Life Sciences & Earth Sciences (AREA)
Biochemistry (AREA)
Molecular Biology (AREA)
Apparatus Associated With Microorganisms And Enzymes (AREA)
Read Only Memory (AREA)

EP02715213.1A 2001-07-26 2002-03-27 Methods and systems for microfluidic processing Expired - Lifetime EP1438567B1 (en)

Priority Applications (1)

Application Number	Priority Date	Filing Date	Title
EP18185265.8A EP3427834A1 (en)	2001-07-26	2002-03-27	Methods and systems for microfluidic processing

Applications Claiming Priority (9)

Application Number	Priority Date	Filing Date	Title
US30763801P	2001-07-26	2001-07-26
US307638P		2001-07-26
US10/014,519 US7192557B2 (en)	2001-03-28	2001-12-14	Methods and systems for releasing intracellular material from cells within microfluidic samples of fluids
US14519		2001-12-14
US14520		2001-12-14
US10/014,520 US7270786B2 (en)	2001-03-28	2001-12-14	Methods and systems for processing microfluidic samples of particle containing fluids
US10/075,371 US7323140B2 (en)	2001-03-28	2002-02-15	Moving microdroplets in a microfluidic device
US75371		2002-02-15
PCT/US2002/009440 WO2003012406A1 (en)	2001-07-26	2002-03-27	Methods and systems for microfluidic processing

Related Child Applications (1)

Application Number	Title	Priority Date	Filing Date
EP18185265.8A Division EP3427834A1 (en)	2001-07-26	2002-03-27	Methods and systems for microfluidic processing

Publications (3)

Publication Number	Publication Date
EP1438567A1 EP1438567A1 (en)	2004-07-21
EP1438567A4 true EP1438567A4 (en)	2010-06-02
EP1438567B1 EP1438567B1 (en)	2018-07-25

Family

ID=27486401

Family Applications (2)

Application Number	Title	Priority Date	Filing Date
EP02715213.1A Expired - Lifetime EP1438567B1 (en)	2001-07-26	2002-03-27	Methods and systems for microfluidic processing
EP18185265.8A Withdrawn EP3427834A1 (en)	2001-07-26	2002-03-27	Methods and systems for microfluidic processing

Family Applications After (1)

Application Number	Title	Priority Date	Filing Date
EP18185265.8A Withdrawn EP3427834A1 (en)	2001-07-26	2002-03-27	Methods and systems for microfluidic processing

Country Status (4)

Country	Link
EP (2)	EP1438567B1 (en)
JP (1)	JP4596776B2 (en)
ES (1)	ES2683698T3 (en)
WO (1)	WO2003012406A1 (en)

Families Citing this family (35)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US6048734A (en)	1995-09-15	2000-04-11	The Regents Of The University Of Michigan	Thermal microvalves in a fluid flow method
US6692700B2 (en)	2001-02-14	2004-02-17	Handylab, Inc.	Heat-reduction methods and systems related to microfluidic devices
US7010391B2 (en)	2001-03-28	2006-03-07	Handylab, Inc.	Methods and systems for control of microfluidic devices
US7829025B2 (en)	2001-03-28	2010-11-09	Venture Lending & Leasing Iv, Inc.	Systems and methods for thermal actuation of microfluidic devices
US7323140B2 (en)	2001-03-28	2008-01-29	Handylab, Inc.	Moving microdroplets in a microfluidic device
US6852287B2 (en)	2001-09-12	2005-02-08	Handylab, Inc.	Microfluidic devices having a reduced number of input and output connections
US8895311B1 (en)	2001-03-28	2014-11-25	Handylab, Inc.	Methods and systems for control of general purpose microfluidic devices
EP2402089A1 (en)	2003-07-31	2012-01-04	Handylab, Inc.	Processing particle-containing samples
US7665303B2 (en)	2004-03-31	2010-02-23	Lifescan Scotland, Ltd.	Method of segregating a bolus of fluid using a pneumatic actuator in a fluid handling circuit
US7059352B2 (en)	2004-03-31	2006-06-13	Lifescan Scotland	Triggerable passive valve for use in controlling the flow of fluid
US7156117B2 (en)	2004-03-31	2007-01-02	Lifescan Scotland Limited	Method of controlling the movement of fluid through a microfluidic circuit using an array of triggerable passive valves
US8852862B2 (en)	2004-05-03	2014-10-07	Handylab, Inc.	Method for processing polynucleotide-containing samples
ES2572382T3 (en)	2004-05-03	2016-05-31	Handylab Inc	A microfluidic device for processing samples containing polynucleotides
US8883490B2 (en)	2006-03-24	2014-11-11	Handylab, Inc.	Fluorescence detector for microfluidic diagnostic system
US10900066B2 (en)	2006-03-24	2021-01-26	Handylab, Inc.	Microfluidic system for amplifying and detecting polynucleotides in parallel
US9040288B2 (en)	2006-03-24	2015-05-26	Handylab, Inc.	Integrated system for processing microfluidic samples, and method of using the same
US11806718B2 (en)	2006-03-24	2023-11-07	Handylab, Inc.	Fluorescence detector for microfluidic diagnostic system
US7998708B2 (en)	2006-03-24	2011-08-16	Handylab, Inc.	Microfluidic system for amplifying and detecting polynucleotides in parallel
WO2008061165A2 (en)	2006-11-14	2008-05-22	Handylab, Inc.	Microfluidic cartridge and method of making same
US8287820B2 (en)	2007-07-13	2012-10-16	Handylab, Inc.	Automated pipetting apparatus having a combined liquid pump and pipette head system
US8182763B2 (en)	2007-07-13	2012-05-22	Handylab, Inc.	Rack for sample tubes and reagent holders
US9618139B2 (en)	2007-07-13	2017-04-11	Handylab, Inc.	Integrated heater and magnetic separator
US8105783B2 (en)	2007-07-13	2012-01-31	Handylab, Inc.	Microfluidic cartridge
US20090136385A1 (en)	2007-07-13	2009-05-28	Handylab, Inc.	Reagent Tube
US8133671B2 (en)	2007-07-13	2012-03-13	Handylab, Inc.	Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
AU2008276211B2 (en)	2007-07-13	2015-01-22	Handylab, Inc.	Polynucleotide capture materials, and methods of using same
US9186677B2 (en)	2007-07-13	2015-11-17	Handylab, Inc.	Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
USD787087S1 (en)	2008-07-14	2017-05-16	Handylab, Inc.	Housing
CN103597358B (en)	2011-04-15	2016-08-17	贝克顿·迪金森公司	Scan real-time microfluid thermal cycler and for the thermal cycle synchronized and the method for scanning optical detection
RU2622432C2 (en)	2011-09-30	2017-06-15	Бектон, Дикинсон Энд Компани	Unified strip for reagents
USD692162S1 (en)	2011-09-30	2013-10-22	Becton, Dickinson And Company	Single piece reagent holder
CN104040238B (en)	2011-11-04	2017-06-27	汉迪拉布公司	Polynucleotides sample preparation apparatus
WO2013116769A1 (en)	2012-02-03	2013-08-08	Becton, Dickson And Company	External files for distribution of molecular diagnostic tests and determination of compatibility between tests
US11666910B2 (en)	2018-11-26	2023-06-06	Hewlett-Packard Development Company, L.P.	Microfluidic devices
CN112023990B (en) *	2019-06-03	2023-06-23	利多(香港)有限公司	Microfluidic detection chip and manufacturing method

Citations (3)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US5635358A (en) *	1992-05-01	1997-06-03	Trustees Of The University Of Pennsylvania	Fluid handling methods for use in mesoscale analytical devices
WO1998022625A1 (en) *	1996-11-20	1998-05-28	The Regents Of The University Of Michigan	Microfabricated isothermal nucleic acid amplification devices and methods
US5922591A (en) *	1995-06-29	1999-07-13	Affymetrix, Inc.	Integrated nucleic acid diagnostic device

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US5639423A (en) *	1992-08-31	1997-06-17	The Regents Of The University Of Calfornia	Microfabricated reactor
US6168948B1 (en) *	1995-06-29	2001-01-02	Affymetrix, Inc.	Miniaturized genetic analysis systems and methods
US6130098A (en) *	1995-09-15	2000-10-10	The Regents Of The University Of Michigan	Moving microdroplets
US6068751A (en) *	1995-12-18	2000-05-30	Neukermans; Armand P.	Microfluidic valve and integrated microfluidic system
US5863502A (en) *	1996-01-24	1999-01-26	Sarnoff Corporation	Parallel reaction cassette and associated devices
JPH10108666A (en) *	1996-10-04	1998-04-28	Asahi Medical Co Ltd	Hollow fiber type incubator
EP0941766B1 (en) *	1998-03-12	2006-12-20	Miltenyi Biotec GmbH	Micro column system for magnetic separation
JP4074713B2 (en) *	1998-07-29	2008-04-09	財団法人川村理化学研究所	Liquid feeding device and manufacturing method thereof
JP2000166535A (en) *	1998-12-10	2000-06-20	Nippon Laser Denshi Kk	Divided microinjector
DE19948473A1 (en) *	1999-10-08	2001-04-12	Nmi Univ Tuebingen	Method and device for measuring cells in a liquid environment
US6272939B1 (en) *	1999-10-15	2001-08-14	Applera Corporation	System and method for filling a substrate with a liquid sample

2002
- 2002-03-27 EP EP02715213.1A patent/EP1438567B1/en not_active Expired - Lifetime
- 2002-03-27 EP EP18185265.8A patent/EP3427834A1/en not_active Withdrawn
- 2002-03-27 WO PCT/US2002/009440 patent/WO2003012406A1/en active Application Filing
- 2002-03-27 JP JP2003517550A patent/JP4596776B2/en not_active Expired - Lifetime
- 2002-03-27 ES ES02715213.1T patent/ES2683698T3/en not_active Expired - Lifetime

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US5635358A (en) *	1992-05-01	1997-06-03	Trustees Of The University Of Pennsylvania	Fluid handling methods for use in mesoscale analytical devices
US5922591A (en) *	1995-06-29	1999-07-13	Affymetrix, Inc.	Integrated nucleic acid diagnostic device
WO1998022625A1 (en) *	1996-11-20	1998-05-28	The Regents Of The University Of Michigan	Microfabricated isothermal nucleic acid amplification devices and methods

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
HANDIQUE K ET AL: "ON-CHIP THERMOPNEUMATIC PRESSURE FOR DISCRETE DROP PUMPING", ANALYTICAL CHEMISTRY, AMERICAN CHEMICAL SOCIETY, US LNKD- DOI:10.1021/AC000711F, vol. 73, no. 8, 15 April 2001 (2001-04-15), pages 1831 - 1838, XP001030281, ISSN: 0003-2700 *
See also references of WO03012406A1 *

Also Published As

Publication number	Publication date
JP2005514718A (en)	2005-05-19
EP3427834A1 (en)	2019-01-16
EP1438567B1 (en)	2018-07-25
WO2003012406A9 (en)	2003-03-20
WO2003012406A1 (en)	2003-02-13
JP4596776B2 (en)	2010-12-15
ES2683698T3 (en)	2018-09-27
EP1438567A1 (en)	2004-07-21

Publication	Publication Date	Title
EP1438567A4 (en)	2010-06-02	Methods and systems for microfluidic processing
GB2397256B (en)	2006-06-28	Apparatus and methods for microfluidic applications
AU2003284055A8 (en)	2004-05-04	Microfluidic systems and components
EP1368909A4 (en)	2009-11-11	Location system and methods
IL158367A0 (en)	2004-05-12	System and method for phytomonitoring
AU2002360239A1 (en)	2003-04-28	Fluid processing device and method
AU2002353107A8 (en)	2003-07-09	Microfluidic devices and methods for two-dimensional separations
AU2002352831A1 (en)	2003-06-10	Methods and systems for analyzing complex biological systems
AU2002353053A1 (en)	2003-07-24	Modular systems and methods for using sample processing devices
GB0414430D0 (en)	2004-07-28	Detection systems and methods
AU2002308687A1 (en)	2002-11-25	Micromagnetic systems and methods for microfluidics
IL160324A0 (en)	2004-07-25	Method and system for profiling biological systems
AU7024801A (en)	2002-01-08	Enhanced sample processing devices, systems and methods
IL159957A0 (en)	2004-06-20	Protein and nucleic expression systems
GB0412418D0 (en)	2004-07-07	Systems and methods for automated commission processing
AU2003261299A8 (en)	2004-02-16	Systems and methods for processing benefits
GB0102245D0 (en)	2001-03-14	Systems/Methods
HK1048870A1 (en)	2003-04-17	Device environment configuration system and deviceenvironment configuration method
EP1690173A4 (en)	2010-04-21	Command processing systems and methods
GB2376073B (en)	2004-10-06	Fluid-gauging systems and methods
EP1417577A4 (en)	2009-08-26	Vcd-on-demand system and method
GB2396558B (en)	2005-07-06	Method and system for processing bio-contaminated articles
GB0106604D0 (en)	2001-05-09	Comminications system and method
GB0203645D0 (en)	2002-04-03	System and process for analysis
EP1418929A4 (en)	2005-01-19	Simmondsin processing methods and products

Legal Events

Date	Code	Title	Description
2004-06-04	PUAI	Public reference made under article 153(3) epc to a published international application that has entered the european phase	Free format text: ORIGINAL CODE: 0009012
2004-07-21	17P	Request for examination filed	Effective date: 20040427
2004-07-21	AK	Designated contracting states	Kind code of ref document: A1 Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE TR
2004-07-21	AX	Request for extension of the european patent	Extension state: AL LT LV MK RO SI
2004-08-04	D17P	Request for examination filed (deleted)
2004-08-11	R17P	Request for examination filed (corrected)	Effective date: 20040427
2004-10-20	RIN1	Information on inventor provided before grant (corrected)	Inventor name: WU, BETTY Inventor name: HANDIQUE, KALYAN Inventor name: GANESAN, KARTHIK Inventor name: PARUNAK, GENE
2005-03-09	RAP1	Party data changed (applicant data changed or rights of an application transferred)	Owner name: HANDYLAB, INC.
2010-06-02	A4	Supplementary search report drawn up and despatched	Effective date: 20100503
2012-04-25	17Q	First examination report despatched	Effective date: 20120323
2016-06-22	RAP1	Party data changed (applicant data changed or rights of an application transferred)	Owner name: HANDYLAB, INC.
2017-12-04	STAA	Information on the status of an ep patent application or granted ep patent	Free format text: STATUS: EXAMINATION IS IN PROGRESS
2018-01-19	REG	Reference to a national code	Ref country code: DE Ref legal event code: R079 Ref document number: 60249534 Country of ref document: DE Free format text: PREVIOUS MAIN CLASS: G01N0021290000 Ipc: B01L0003000000
2018-02-05	GRAP	Despatch of communication of intention to grant a patent	Free format text: ORIGINAL CODE: EPIDOSNIGR1
2018-02-05	STAA	Information on the status of an ep patent application or granted ep patent	Free format text: STATUS: GRANT OF PATENT IS INTENDED
2018-02-28	RIC1	Information provided on ipc code assigned before grant	Ipc: B01L 3/00 20060101AFI20180119BHEP
2018-03-07	INTG	Intention to grant announced	Effective date: 20180206
2018-06-06	GRAJ	Information related to disapproval of communication of intention to grant by the applicant or resumption of examination proceedings by the epo deleted	Free format text: ORIGINAL CODE: EPIDOSDIGR1
2018-06-06	STAA	Information on the status of an ep patent application or granted ep patent	Free format text: STATUS: EXAMINATION IS IN PROGRESS
2018-06-18	GRAR	Information related to intention to grant a patent recorded	Free format text: ORIGINAL CODE: EPIDOSNIGR71
2018-06-18	GRAS	Grant fee paid	Free format text: ORIGINAL CODE: EPIDOSNIGR3
2018-06-18	STAA	Information on the status of an ep patent application or granted ep patent	Free format text: STATUS: GRANT OF PATENT IS INTENDED
2018-06-22	GRAA	(expected) grant	Free format text: ORIGINAL CODE: 0009210
2018-06-22	STAA	Information on the status of an ep patent application or granted ep patent	Free format text: STATUS: THE PATENT HAS BEEN GRANTED
2018-07-11	INTC	Intention to grant announced (deleted)
2018-07-25	AK	Designated contracting states	Kind code of ref document: B1 Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE TR
2018-07-25	INTG	Intention to grant announced	Effective date: 20180618
2018-07-25	REG	Reference to a national code	Ref country code: GB Ref legal event code: FG4D
2018-07-31	REG	Reference to a national code	Ref country code: CH Ref legal event code: EP
2018-08-15	REG	Reference to a national code	Ref country code: AT Ref legal event code: REF Ref document number: 1021175 Country of ref document: AT Kind code of ref document: T Effective date: 20180815
2018-08-16	REG	Reference to a national code	Ref country code: DE Ref legal event code: R096 Ref document number: 60249534 Country of ref document: DE
2018-08-22	REG	Reference to a national code	Ref country code: IE Ref legal event code: FG4D
2018-09-27	REG	Reference to a national code	Ref country code: ES Ref legal event code: FG2A Ref document number: 2683698 Country of ref document: ES Kind code of ref document: T3 Effective date: 20180927
2018-10-31	REG	Reference to a national code	Ref country code: NL Ref legal event code: FP
2019-01-15	REG	Reference to a national code	Ref country code: AT Ref legal event code: MK05 Ref document number: 1021175 Country of ref document: AT Kind code of ref document: T Effective date: 20180725
2019-01-31	PG25	Lapsed in a contracting state [announced via postgrant information from national office to epo]	Ref country code: AT Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT Effective date: 20180725 Ref country code: GR Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT Effective date: 20181026 Ref country code: FI Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT Effective date: 20180725 Ref country code: SE Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT Effective date: 20180725
2019-01-31	REG	Reference to a national code	Ref country code: CH Ref legal event code: PK Free format text: BERICHTIGUNGEN
2019-02-13	RIC2	Information provided on ipc code assigned after grant	Ipc: B01L 3/00 20060101AFI20180119BHEP
2019-04-26	REG	Reference to a national code	Ref country code: DE Ref legal event code: R097 Ref document number: 60249534 Country of ref document: DE
2019-05-31	PG25	Lapsed in a contracting state [announced via postgrant information from national office to epo]	Ref country code: DK Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT Effective date: 20180725
2019-05-31	PLBE	No opposition filed within time limit	Free format text: ORIGINAL CODE: 0009261
2019-05-31	STAA	Information on the status of an ep patent application or granted ep patent	Free format text: STATUS: NO OPPOSITION FILED WITHIN TIME LIMIT
2019-07-03	26N	No opposition filed	Effective date: 20190426
2019-10-31	PG25	Lapsed in a contracting state [announced via postgrant information from national office to epo]	Ref country code: MC Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT Effective date: 20180725
2019-10-31	REG	Reference to a national code	Ref country code: CH Ref legal event code: PL
2019-11-29	PG25	Lapsed in a contracting state [announced via postgrant information from national office to epo]	Ref country code: LU Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES Effective date: 20190327
2019-12-05	REG	Reference to a national code	Ref country code: BE Ref legal event code: MM Effective date: 20190331
2020-01-31	PG25	Lapsed in a contracting state [announced via postgrant information from national office to epo]	Ref country code: CH Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES Effective date: 20190331 Ref country code: LI Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES Effective date: 20190331 Ref country code: IE Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES Effective date: 20190327
2020-02-28	PG25	Lapsed in a contracting state [announced via postgrant information from national office to epo]	Ref country code: BE Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES Effective date: 20190331
2020-03-31	PG25	Lapsed in a contracting state [announced via postgrant information from national office to epo]	Ref country code: TR Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT Effective date: 20180725
2020-06-30	PG25	Lapsed in a contracting state [announced via postgrant information from national office to epo]	Ref country code: PT Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT Effective date: 20181125
2021-04-30	PGFP	Annual fee paid to national office [announced via postgrant information from national office to epo]	Ref country code: NL Payment date: 20210219 Year of fee payment: 20 Ref country code: FR Payment date: 20210218 Year of fee payment: 20
2021-05-31	PG25	Lapsed in a contracting state [announced via postgrant information from national office to epo]	Ref country code: CY Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT Effective date: 20180725
2021-05-31	PGFP	Annual fee paid to national office [announced via postgrant information from national office to epo]	Ref country code: GB Payment date: 20210219 Year of fee payment: 20 Ref country code: DE Payment date: 20210217 Year of fee payment: 20
2021-08-31	PGFP	Annual fee paid to national office [announced via postgrant information from national office to epo]	Ref country code: ES Payment date: 20210401 Year of fee payment: 20
2021-10-29	PGFP	Annual fee paid to national office [announced via postgrant information from national office to epo]	Ref country code: IT Payment date: 20210217 Year of fee payment: 20
2022-03-27	REG	Reference to a national code	Ref country code: DE Ref legal event code: R071 Ref document number: 60249534 Country of ref document: DE
2022-03-30	REG	Reference to a national code	Ref country code: NL Ref legal event code: MK Effective date: 20220326
2022-04-20	REG	Reference to a national code	Ref country code: GB Ref legal event code: PE20 Expiry date: 20220326
2022-04-29	PG25	Lapsed in a contracting state [announced via postgrant information from national office to epo]	Ref country code: GB Free format text: LAPSE BECAUSE OF EXPIRATION OF PROTECTION Effective date: 20220326
2022-09-02	REG	Reference to a national code	Ref country code: ES Ref legal event code: FD2A Effective date: 20220902
2022-10-31	PG25	Lapsed in a contracting state [announced via postgrant information from national office to epo]	Ref country code: ES Free format text: LAPSE BECAUSE OF EXPIRATION OF PROTECTION Effective date: 20220328