[go: up one dir, main page]

EP1318869A1 - Dispositif d'incubation - Google Patents

Dispositif d'incubation

Info

Publication number
EP1318869A1
EP1318869A1 EP01962965A EP01962965A EP1318869A1 EP 1318869 A1 EP1318869 A1 EP 1318869A1 EP 01962965 A EP01962965 A EP 01962965A EP 01962965 A EP01962965 A EP 01962965A EP 1318869 A1 EP1318869 A1 EP 1318869A1
Authority
EP
European Patent Office
Prior art keywords
holding frame
plate
titer
titer plate
cover plate
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP01962965A
Other languages
German (de)
English (en)
Inventor
Holger Deppe
Beate Diefenbach
Andreas Willems
Hanns Wurziger
Alexander Gross
Gregor Schlingloff
Andreas Schober
Dirk Tomandl
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Institut fuer Physikalische Hochtechnologie eV
Merck Patent GmbH
Original Assignee
Institut fuer Physikalische Hochtechnologie eV
Merck Patent GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Institut fuer Physikalische Hochtechnologie eV, Merck Patent GmbH filed Critical Institut fuer Physikalische Hochtechnologie eV
Publication of EP1318869A1 publication Critical patent/EP1318869A1/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
    • B01L3/50851—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates specially adapted for heating or cooling samples
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
    • B01L3/50853—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates with covers or lids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0689—Sealing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/14—Process control and prevention of errors
    • B01L2200/142—Preventing evaporation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0829—Multi-well plates; Microtitration plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/10—Means to control humidity and/or other gases

Definitions

  • the invention relates to a device for the parallel incubation of solutions.
  • reaction containers are usually combined in a matrix to form reaction units.
  • a still further miniaturization of the individual reaction containers is achieved by using small recessed cavities in a plate as reaction cavities.
  • titer plates can, for example, consist of a photo-structured glass plate or a surface-treated plastic plate consist.
  • Titer plates are also known in which a chip made of silicon has a large number of small, regularly arranged cavities.
  • the individual reaction cavities have a complex shape.
  • it is also referred to as a microtiter plate or nanotiter plate.
  • the individual reaction cavities have volumes usable for samples in the range from milliliters to a few microliters, with nanotiter plates volumes in the nanoliter range are achieved.
  • the miniaturization achieved in this way enables an increasing parallelization of individual process steps and at the same time reduces the constantly incurring costs for the procurement or disposal of the chemical substances and solvents used.
  • the object of the invention is therefore to design a device of the type mentioned in the introduction in such a way that evaporation effects from individual reaction cavities are prevented with the least possible production outlay.
  • the device should also enable extensive automation of the necessary process steps.
  • the solution to this problem according to the invention is based on a device for the parallel incubation of solutions with a holding frame receiving a titer plate and with a the titer plate in the holding frame tightly sealing, pressable cover plate.
  • the holding frame has a continuous recess below the titer plate which is adapted to the dimensions of the titer plate and which can be tightly closed by a stamp which is movable perpendicularly to the plane of the titer plate.
  • An essential prerequisite for the efficient and cost-effective implementation of the parallel incubation of biological or chemical samples is extensive automation of all of them Process steps. It is therefore possible with a titer plate in which the individual reaction cavities are limited at the bottom only by a sieve structure bottom, to aspirate the sample material or the solvent used downwards. Due to the correspondingly adapted recess in the holding frame, an inserted titer plate can be filled from above and emptied downwards without the titer plate having to be moved or removed from the holding frame.
  • the movable stamp is placed just below the titer plate.
  • the movable plunger reduces the closed air volume underneath the titer plate and thus prevents evaporation effects and transport phenomena of the filled sample substances and the solvent.
  • the stamp can be automatically moved to the designated position and removed again.
  • the entire holding frame with the inserted titer plates can be removed and used in other laboratory devices.
  • an embodiment of the inventive concept provides that the cover plate can be pressed tightly onto the holding frame by screw, spring or tensioning devices. Depending on the particular properties of the sample and the solvent used, the minimum contact pressure of the cover plate may vary. At the same time, however, a simple and quick-release locking mechanism is advantageous for automated handling of the device. Especially by screwing the cover plate to the holding frame, the titer plate is sealed sufficiently even during a long-term incubation at a high temperature.
  • the holding frame has devices for temperature control. Many biological or chemical processes are affected by the ambient temperature. A controlled and reproducible incubation of the samples is therefore often only possible if the temperature of the titer plate can be specified. If an unconditioned device for incubation is introduced into an incubator at an already elevated temperature, it takes a correspondingly long time e, depending on the temperature difference, for an equilibrium to be established. Even small temperature gradients within a titer plate favor evaporation effects and transport phenomena and lead to a mutual influence of neighboring reaction cavities.
  • Devices for temperature control located in the holding frame ensure a uniform and constant temperature of the directly lying titer plate. Forced temperature changes, such as occur at the beginning and at the end of an incubation, can be achieved much faster and more controlled. It is preferably provided that the cover plate has devices for temperature control.
  • the temperature of the titer plate can also be regulated via a heatable cover plate. It is also possible to suppress condensation of sample material and solvent on the underside of the cover plate by increasing the temperature of the cover plate. This particularly prevents vaporized solvent from depositing on the underside of the cover plate and individual drops then falling uncontrollably onto the titer plate.
  • fogging of the cover plate can be prevented by a correspondingly predetermined temperature of the cover plate, so that the individual reaction cavities of the titer plate are freely visible at all times. This enables, for example, optical analysis methods during an incubation without the cover plate having to be removed beforehand.
  • the holding frame has, in addition to the titer plate, channel-like recesses for receiving liquid solvent.
  • a certain amount of liquid evaporates, so that an equilibrium is established with the then sufficiently saturated atmosphere directly above the titer plate.
  • liquid solvent is poured into the trough-like recesses before inserting or filling the titer plates, the trough-like recesses will carry The amount of liquid contributes equally to evaporation.
  • the dimensions of the channel-like recesses can be chosen so that the liquid necessary for a saturated atmosphere evaporates predominantly from the channel-like recesses. Evaporation effects from individual reaction cavities of the titer plate can thereby be additionally reduced or almost completely prevented.
  • the cover plate has recesses above the titer plate for receiving solvents.
  • a recess for example, a nonwoven soaked with solvent can be attached.
  • a relatively large supply of solvent is available within the small closed air volume.
  • the surface structure of the fleece promotes evaporation of the liquid in it. At the same time, unwanted dripping onto the titer plate is prevented.
  • a plurality of titer plates can be inserted next to one another in the holding frame and sealed tightly by a press-on cover plate. Due to the small dimensions of individual reaction cavities, a relatively large number of reaction cavities can be arranged even on a small titer plate. The manufacturing effort and the manufacturing costs increase sharply with increasing size of the titer plate. It is therefore in principle advantageous to use several small and more manageable titer plates at the same time instead of a single, as large as possible titer plate. This way you can increase it with just a little A very large number of individual reaction cavities can be used in parallel.
  • the common cover plate pressed onto the holding frame encloses each titer plate individually. A liquid or gas exchange between neighboring titer plates is not possible. In the case of several small titer plates, the air volume divided into segments above each titer plate is correspondingly small, so that only a little liquid can evaporate.
  • the movable stamps assigned to a titer plate are designed as stem-shaped configurations of a common base plate.
  • the continuous recesses in the holding frame underneath each titer plate can be released and closed again easily and completely automatically. Complex devices for the controlled alignment and movement of individual stamps are therefore unnecessary.
  • the respective stroke is common to all stamps, so that each titer plate has essentially identical ambient conditions during the incubation.
  • 1 shows a section through a holding frame receiving several titer plates
  • 2 shows a section through a similarly designed holding frame with an attached cover plate
  • FIG. 3 shows a section through a holding frame with a plurality of cutouts for holding liquids
  • Fig. 4 shows a section through a holding frame, in which a nonwoven soaked with solvent is attached in a recess below the titer plate
  • FIG. 5 shows the holding frame shown in FIG. 4, the fleece soaked with liquid being fastened in a recess on the underside of the cover plate.
  • the device for the parallel incubation of solutions shown in FIGS. 1 and 2 has a plurality of titer plates 2 inserted into a holding frame 1. Each titer plate 2 rests on side projections 3 of the holding frame 1. Each titer plate 2 is individually completely surrounded by the holding frame 1 on its side faces. The top of the titer plates 2 is located slightly below the upper edge of the holding frame 1. From above, a continuous cover plate 4 is pressed onto the holding frame 1, so that each titer plate 2 is enclosed separately. Several seals 5 are attached between the holding frame 1 and the cover plate 4. As a result, sufficient sealing of the individual titer plates 2 is achieved even with a relatively low contact pressure of the cover plate 4 on the holding frame 1.
  • the cover plate 4, the holding frame 1 and the one-piece base plate 7 are pressed together with continuous screw connections 8.
  • This can be achieved in a simple manner, for example by means of a threaded rod and thumbscrews screwed on from both sides.
  • the distance of the continuous screw connections 8 from one another can be adapted to the contact pressure of the cover plate 4 on the holding frame 1 which is at least necessary for the adequate sealing.
  • a continuous screw connection 8 is provided at the corners of each titer plate 2.
  • the one-piece base plate 7 can be made of dimensionally stable material, so that its attachment to the holding frame 1 requires little effort.
  • FIG. 2 shows a slightly modified embodiment of the device shown in FIG. 1, in which a plurality of continuous screw connections 8 are provided only on the outer sides of the holding frame 1.
  • the cover plate 4 can additionally be pressed onto the holding frame 1 with screw connections 9 on one side.
  • a one-piece base plate 7 with stem-shaped formations 6 the use of several stamps conceivable, which are attached to a common base plate 7.
  • the holding frame 1 'shown in FIG. 3 has groove-like recesses 10 which are arranged surrounding the inserted titer plate 2.
  • the capacity of the channel-like recesses 10 is dimensioned such that the proportion of the liquid evaporating from a filled titer plate 2 is largely reduced.
  • a movable plunger 11, which closes the continuous recess of the holding frame 1 'below the titer plate 2, has a recess on the side facing the titer plate 2, in which there is a liquid-soaked fleece 12. While the liquid from the channel-like recesses 10 of the holding frame 1 'mainly evaporates into the air volume above the titer plate 2, the space below the titer plate 2 is essentially moistened by the liquid-soaked fleece 12 on the upper side of the movable plunger 11.
  • a movable stamp 11 without a recess and liquid-soaked fleece 12 attached therein is used. Such a movable stamp can then, for example, be pressed resiliently onto the titer plate 2 which can be fastened in the holding frame 1 '. This makes it possible for special applications to close openings on the underside of the titer plate 2 by means of the movable stamp 11.
  • a suitable choice of the spring force enables the underside of the titer plate 2 to be sealed as tightly as possible, but at the same time damage to the usually sensitive titer plate 2 by excessive pressure forces is avoided.
  • the movable plunger 11 has devices for temperature control. A movable plunger 11 located directly below the titer plate 2 or even in contact with it can be advantageous due to its size and position for fast and precise temperature control of the titer plate 2.
  • FIG. 4 and 5 show an essentially identical holding frame 1 ". Below the inserted titer plate 2 there is a flat recess in which, as shown in FIG. 4, a liquid-soaked fleece 12 is attached. The liquid-soaked fleece 12 can but can also be fastened instead or additionally in a recess in the cover plate 4 'provided for this purpose.
  • the cover plate 4' is pressed onto the holding frame 1 "by means of screw, spring or tensioning devices not shown in the figures. Seals 5 attached between the cover plate 4 ′ and the holding frame 1 ′′ prevent gas or liquid exchange between adjacent titer plates 2 or with the surroundings.

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Analytical Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

L'invention concerne un dispositif utilisé pour l'incubation parallèle de solutions, qui comporte des cadres de retenue (1) dans lesquels peuvent être placées une ou plusieurs plaques de titrage (2). Une plaque de recouvrement (4) pouvant être apposée par pression permet de fermer séparément chaque plaque de titrage (2) et d'éviter de ce fait l'émergence d'effets d'évaporation et de phénomènes de transport indésirables. Des garnitures d'étanchéité (5) sont disposées entre la plaque de recouvrement (4) et le cadre de retenue (1). Il est prévu sous les plaques de titrage (2), des évidements traversants dans le cadre de retenue (1), qui sont fermés par une plaque de base (7) monobloc commune, qui présente des déformations saillantes (6) de type poinçons. Il est possible de placer des dispositifs pour réguler la température, aussi bien dans la plaque de recouvrement (4) et dans le cadre de retenue (1), que dans la plaque de base (7). Le cadre de retenue (1) comporte des évidements sous forme de rigoles pour recevoir un solvant fluide.
EP01962965A 2000-09-19 2001-08-23 Dispositif d'incubation Withdrawn EP1318869A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE10046224A DE10046224A1 (de) 2000-09-19 2000-09-19 Inkubationsvorrichtung
DE10046224 2000-09-19
PCT/EP2001/009747 WO2002024336A1 (fr) 2000-09-19 2001-08-23 Dispositif d'incubation

Publications (1)

Publication Number Publication Date
EP1318869A1 true EP1318869A1 (fr) 2003-06-18

Family

ID=7656710

Family Applications (1)

Application Number Title Priority Date Filing Date
EP01962965A Withdrawn EP1318869A1 (fr) 2000-09-19 2001-08-23 Dispositif d'incubation

Country Status (7)

Country Link
US (1) US20030235517A1 (fr)
EP (1) EP1318869A1 (fr)
JP (1) JP2004508842A (fr)
KR (1) KR20030059144A (fr)
AU (1) AU2001284031A1 (fr)
DE (1) DE10046224A1 (fr)
WO (1) WO2002024336A1 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109253811A (zh) * 2017-07-15 2019-01-22 浙江新世纪工程检测有限公司 一种建筑工程用保温材料检测的设备及其方法

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6893877B2 (en) 1998-01-12 2005-05-17 Massachusetts Institute Of Technology Methods for screening substances in a microwell array
AU756982B2 (en) 1999-03-19 2003-01-30 Life Technologies Corporation Multi-through hole testing plate for high throughput screening
US20020151040A1 (en) 2000-02-18 2002-10-17 Matthew O' Keefe Apparatus and methods for parallel processing of microvolume liquid reactions
CA2400644C (fr) 2000-02-18 2009-07-14 Board Of Trustees Of The Leland Stanford Junior University Appareils et procedes de conduite en parallele de reactions sur des microvolumes
JP3707445B2 (ja) * 2002-04-10 2005-10-19 松下電器産業株式会社 マイクロタイタープレート
US8277753B2 (en) 2002-08-23 2012-10-02 Life Technologies Corporation Microfluidic transfer pin
AU2003302264A1 (en) 2002-12-20 2004-09-09 Biotrove, Inc. Assay apparatus and method using microfluidic arrays
EP1735097B1 (fr) 2004-03-12 2016-11-30 Life Technologies Corporation Chargement d'ensembles d'echantillons de l'ordre du nanolitre
DE102005027407B3 (de) * 2005-06-13 2006-11-09 Eppendorf Ag Thermocycler
WO2008094744A1 (fr) * 2007-01-26 2008-08-07 Kalypsys, Inc. Couvercle de plaque multipuits avec jupe de protection
KR100870982B1 (ko) * 2007-05-03 2008-12-01 주식회사 바이오트론 다중 생물 반응 장치
CH708820A1 (de) * 2013-11-07 2015-05-15 Tecan Trading Ag Inkubationskassette.
JP6156933B2 (ja) * 2014-01-22 2017-07-05 国立大学法人 筑波大学 細胞培養用デバイス
CN106999926A (zh) * 2014-06-02 2017-08-01 安捷伦科技有限公司 用于分析生物样本的单列微板系统和载体
KR101983943B1 (ko) * 2018-05-10 2019-05-29 공주대학교 산학협력단 세포 배양 용기 케이스
US20210284947A1 (en) * 2020-03-12 2021-09-16 Ricoh Company, Ltd. Drying suppression device and cell culture kit

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3942952A (en) * 1974-08-22 1976-03-09 The Perkin-Elmer Corporation Sample platter moisturizing system
US4676951A (en) * 1985-07-01 1987-06-30 American Hospital Supply Corp. Automatic specimen analyzing system
FI852736A0 (fi) * 1985-07-10 1985-07-10 Labsystems Oy Termosterbar kyvettenhet.
NL8801127A (nl) * 1988-04-29 1989-11-16 Univ Utrecht Inrichting voor het op een bepaalde temperatuur brengen en houden van een monsterhouder.
US5108704A (en) * 1988-09-16 1992-04-28 W. R. Grace & Co.-Conn. Microfiltration apparatus with radially spaced nozzles
EP0388159A3 (fr) * 1989-03-15 1991-06-12 Seiko Instruments Inc. Dispositif pour le scellement d'un liquide dans des cavités
CA2031912A1 (fr) * 1989-12-22 1991-06-23 Robert Fred Pfost Couvercle chauffe
KR100236506B1 (ko) * 1990-11-29 2000-01-15 퍼킨-엘머시터스인스트루먼츠 폴리머라제 연쇄 반응 수행 장치
US5789251A (en) * 1994-06-16 1998-08-04 Astle; Thomas W. Multi-well bioassay tray with evaporation protection and method of use
BE1010984A3 (nl) * 1995-02-17 1999-03-02 Praet Peter Van Incubator voor microtiter plaat.
US6171780B1 (en) * 1997-06-02 2001-01-09 Aurora Biosciences Corporation Low fluorescence assay platforms and related methods for drug discovery
JP3062481B2 (ja) * 1997-11-19 2000-07-10 グルポ グリフォルス，エス．エー． 実験室試験を自動的に行う装置
US6103199A (en) * 1998-09-15 2000-08-15 Aclara Biosciences, Inc. Capillary electroflow apparatus and method
US6046703A (en) * 1998-11-10 2000-04-04 Nutex Communication Corp. Compact wireless transceiver board with directional printed circuit antenna

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See references of WO0224336A1 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109253811A (zh) * 2017-07-15 2019-01-22 浙江新世纪工程检测有限公司 一种建筑工程用保温材料检测的设备及其方法
CN109253811B (zh) * 2017-07-15 2020-06-16 浙江新世纪工程检测有限公司 一种建筑工程用保温材料检测的设备及其方法

Also Published As

Publication number Publication date
JP2004508842A (ja) 2004-03-25
KR20030059144A (ko) 2003-07-07
AU2001284031A1 (en) 2002-04-02
WO2002024336A1 (fr) 2002-03-28
DE10046224A1 (de) 2002-03-28
US20030235517A1 (en) 2003-12-25

Similar Documents

Publication Publication Date Title
WO2002024336A1 (fr) Dispositif d'incubation
DE60034033T2 (de) Vorrichtung zum screening von kristallisierungsbedingungen in lösungen zur kristallzüchtung
DE19941905C2 (de) Probenkammer zur Flüssigkeitsbehandlung biologischer Proben
DE60211155T2 (de) Mehrfachlochtestvorrichtung
EP2098294B1 (fr) Porte-échantillons destiné à la préparation d'une multitude de récipients d'échantillons, notamment des petits tuyaux d'échantillons RMN
DE69615655T2 (de) Biologische analytische vorrichtung mit verbesserter verhinderung von kontamination
DE69311568T2 (de) Vorrichtung und verfahren zur ausführung von insbesondere vergleichenden tests
EP0172896B1 (fr) Procede et installation pour amener simultanement une pluralite d'echantillons de liquides sur une lame porte-objets
DE102014018308A1 (de) Temperierkörper für eine Multiwell-Platte und Verfahren und Vorrichtung zum Einfrieren und/oder Auftauen von biologischen Proben
DE19920811A1 (de) Vorrichtung zur Durchführung von Untersuchungen an Zellkulturen
AT500523A1 (de) Vorrichtung zur proteinkristallisation
DE19842797C1 (de) Probenhalterung für eine partikelförmige flüssigkeitshaltige Materialprobe
DD239473A1 (de) Probentraeger zur diskreten analyse fluessiger analysensaetze
DE69119647T2 (de) Kapillar-Impfgerät und Anordnung und Verfahren zur Impfung einer Vielzahl von Testfeldern
DE10020771A1 (de) Pipettiervorrichtung
DE4016617A1 (de) Objekttraeger fuer nasse oder feuchte praeparate, z. b. blut
EP1397201B2 (fr) Recipient de reaction servant a produire des echantillons
EP1341611B1 (fr) Porte-echantillon, son couvercle et procede de preparation d'analyse
EP0530186B1 (fr) Porte-objets pour preparations a teneur en eau, par exemple, de sang
AT406310B (de) Vorrichtung zur messung der migrationsfähigkeit von amöboid beweglichen zellen
AT500167B1 (de) Reaktionsgefäss
DE10025698A1 (de) Vorrichtung für die parallele Mikrosynthese
DE10147513B4 (de) Verfahren zur Minimierung der Verdunstung in Probenträgern
DE102017010815A1 (de) Vorrichtung und Verfahren für die parallele Handhabung, Beobachtung und Kontrolle von gewebeähnlichen Zellverbänden
DE7916943U1 (fr)

Legal Events

Date Code Title Description
PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

17P Request for examination filed

Effective date: 20030117

AK Designated contracting states

Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE TR

AX Request for extension of the european patent

Extension state: AL LT LV MK RO SI

RIN1 Information on inventor provided before grant (corrected)

Inventor name: TOMANDL, DIRK

Inventor name: SCHOBER, ANDREAS

Inventor name: SCHLINGLOFF, GREGOR

Inventor name: GROSS, ALEXANDER

Inventor name: WURZIGER, HANNS

Inventor name: WILLEMS, ANDREAS

Inventor name: DIEFENBACH, BEATE

Inventor name: DEPPE, HOLGER

17Q First examination report despatched

Effective date: 20030618

RIN1 Information on inventor provided before grant (corrected)

Inventor name: TOMANDL, DIRK

Inventor name: SCHOBER, ANDREAS

Inventor name: SCHLINGLOFF, GREGOR

Inventor name: GROSS, ALEXANDER

Inventor name: WURZIGER, HANNS

Inventor name: WILLEMS, ANDREAS

Inventor name: DIEFENBACH, BEATE

Inventor name: DEPPE, HOLGER

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE APPLICATION IS DEEMED TO BE WITHDRAWN

18D Application deemed to be withdrawn

Effective date: 20031229