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EP1214445A4 - Compositions for the detection of enzyme activity in biological samples and methods of use thereof - Google Patents

Compositions for the detection of enzyme activity in biological samples and methods of use thereof

Info

Publication number
EP1214445A4
EP1214445A4 EP00961782A EP00961782A EP1214445A4 EP 1214445 A4 EP1214445 A4 EP 1214445A4 EP 00961782 A EP00961782 A EP 00961782A EP 00961782 A EP00961782 A EP 00961782A EP 1214445 A4 EP1214445 A4 EP 1214445A4
Authority
EP
European Patent Office
Prior art keywords
compositions
detection
methods
enzyme activity
biological samples
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP00961782A
Other languages
German (de)
French (fr)
Other versions
EP1214445A1 (en
Inventor
Akira Komoriya
Beverly S Packard
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Oncoimmunin Inc
Original Assignee
Oncoimmunin Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/394,019 external-priority patent/US6936687B1/en
Application filed by Oncoimmunin Inc filed Critical Oncoimmunin Inc
Publication of EP1214445A1 publication Critical patent/EP1214445A1/en
Publication of EP1214445A4 publication Critical patent/EP1214445A4/en
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/533—Production of labelled immunochemicals with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)
    • G01N2333/95—Proteinases, i.e. endopeptidases (3.4.21-3.4.99)
    • G01N2333/964—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue
    • G01N2333/96425—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Urology & Nephrology (AREA)
  • Analytical Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
EP00961782A 1999-09-10 2000-09-11 Compositions for the detection of enzyme activity in biological samples and methods of use thereof Withdrawn EP1214445A4 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US09/394,019 US6936687B1 (en) 1997-02-20 1999-09-10 Compositions for the detection of enzyme activity in biological samples and methods of use thereof
US394019 1999-09-10
PCT/US2000/024882 WO2001018238A1 (en) 1999-09-10 2000-09-11 Compositions for the detection of enzyme activity in biological samples and methods of use thereof

Publications (2)

Publication Number Publication Date
EP1214445A1 EP1214445A1 (en) 2002-06-19
EP1214445A4 true EP1214445A4 (en) 2007-01-24

Family

ID=23557207

Family Applications (1)

Application Number Title Priority Date Filing Date
EP00961782A Withdrawn EP1214445A4 (en) 1999-09-10 2000-09-11 Compositions for the detection of enzyme activity in biological samples and methods of use thereof

Country Status (5)

Country Link
EP (1) EP1214445A4 (en)
JP (1) JP2003508080A (en)
AU (1) AU7368800A (en)
CA (1) CA2384021A1 (en)
WO (1) WO2001018238A1 (en)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6037137A (en) 1997-02-20 2000-03-14 Oncoimmunin, Inc. Fluorogenic peptides for the detection of protease activity
US7312302B2 (en) 1997-02-20 2007-12-25 Oncolmmunin, Inc. Compositions for the detection of enzyme activity in biological samples and methods of use thereof
US6893868B2 (en) 1997-02-20 2005-05-17 Onco Immunin, Inc. Homo-doubly labeled compositions for the detection of enzyme activity in biological samples
WO2003029480A2 (en) * 2001-08-31 2003-04-10 The Government Of The United States Of America, Asrepresented By The Secretary Of The Department Of Health And Human Services Measurements of multiple molecules using a cryoarray
GB0200479D0 (en) * 2002-01-09 2002-02-27 Medical Res Council Fluorogenic protease substrates
US7927871B2 (en) 2002-01-29 2011-04-19 Oncoimmunin, Inc. Visualization and quantitation of cellular cytotoxicity using cell-permeable fluorogenic protease substrates and caspase activity indicator markers
GB0311948D0 (en) * 2003-05-23 2003-06-25 Stockport Innovation Ltd Light emitting probes
WO2009104741A1 (en) * 2008-02-22 2009-08-27 和光純薬工業株式会社 Substrate for assaying β-glucan and/or endotoxin and assay method
EP2790736B1 (en) * 2011-12-12 2018-01-31 Oncoimmunin, Inc. In vivo delivery of oligonucleotides
EP4397672A4 (en) 2021-08-31 2025-02-26 Fujifilm Corp COMPOUND AND MARKED BIOMATERIAL WITH IT

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998037226A1 (en) * 1997-02-20 1998-08-27 Oncoimmunin, Inc. Compositions for the detection of enzyme activity in biological samples and methods of use thereof
WO2000004914A1 (en) * 1998-07-21 2000-02-03 Cytovia, Inc. Novel fluorescence dyes and their applications for whole cell fluorescence screening assays for caspases, peptidases, proteases and other enzymes and the use thereof

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5723288A (en) * 1994-05-06 1998-03-03 The University Of North Carolina At Chapel Hill Method of fluorescent detection of nucleic acids and cytoskeleton elements using bis-dicationic aryl furans, and kits useful therefor
US5804395A (en) * 1995-12-01 1998-09-08 The United States Of America As Represented By The Secretary Of The Navy Fluorescence polarization assays of enzymes and substrates therefore

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998037226A1 (en) * 1997-02-20 1998-08-27 Oncoimmunin, Inc. Compositions for the detection of enzyme activity in biological samples and methods of use thereof
WO2000004914A1 (en) * 1998-07-21 2000-02-03 Cytovia, Inc. Novel fluorescence dyes and their applications for whole cell fluorescence screening assays for caspases, peptidases, proteases and other enzymes and the use thereof

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
MATAYOSHI E D ET AL: "NOVEL FLUOROGENIC SUBSTRATES FOR ASSAYING RETROVIRAL PROTEASES BY RESONANCE ENERGY TRANSFER", SCIENCE, AMERICAN ASSOCIATION FOR THE ADVANCEMENT OF SCIENCE,, US, vol. 24, 23 February 1990 (1990-02-23), pages 954 - 958, XP002056345, ISSN: 0036-8075 *
See also references of WO0118238A1 *
TOTH M V ET AL: "A SIMPLE CONTINUOUS FLUOROMETRIC ASSAY FOR HIV PROTEASE", INTERNATIONAL JOURNAL OF PEPTIDE AND PROTEIN RESEARCH, MUNKSGAARD, COPENHAGEN, DK, vol. 36, no. 6, 1990, pages 544 - 550, XP001160870, ISSN: 0367-8377 *
WANG G T ET AL: "A continuous fluorescence assay of renin activity.", ANALYTICAL BIOCHEMISTRY. 1 MAY 1993, vol. 210, no. 2, 1 May 1993 (1993-05-01), pages 351 - 359, XP002406820, ISSN: 0003-2697 *

Also Published As

Publication number Publication date
JP2003508080A (en) 2003-03-04
AU7368800A (en) 2001-04-10
WO2001018238A1 (en) 2001-03-15
EP1214445A1 (en) 2002-06-19
CA2384021A1 (en) 2001-03-15

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Legal Events

Date Code Title Description
PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

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17P Request for examination filed

Effective date: 20020410

AK Designated contracting states

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AX Request for extension of the european patent

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A4 Supplementary search report drawn up and despatched

Effective date: 20061228

17Q First examination report despatched

Effective date: 20080226

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE APPLICATION IS DEEMED TO BE WITHDRAWN

18D Application deemed to be withdrawn

Effective date: 20080909