EP1210451A1 - Schnellverfahren zum messen komplexer kohlenhydrate in säugergeweben - Google Patents
Schnellverfahren zum messen komplexer kohlenhydrate in säugergewebenInfo
- Publication number
- EP1210451A1 EP1210451A1 EP00955193A EP00955193A EP1210451A1 EP 1210451 A1 EP1210451 A1 EP 1210451A1 EP 00955193 A EP00955193 A EP 00955193A EP 00955193 A EP00955193 A EP 00955193A EP 1210451 A1 EP1210451 A1 EP 1210451A1
- Authority
- EP
- European Patent Office
- Prior art keywords
- complex carbohydrates
- measuring complex
- rapid method
- measuring
- measurement
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 238000000034 method Methods 0.000 title claims abstract description 77
- 150000001720 carbohydrates Chemical class 0.000 title claims abstract description 40
- 235000014633 carbohydrates Nutrition 0.000 title claims abstract description 40
- 229920002527 Glycogen Polymers 0.000 claims abstract description 42
- 229940096919 glycogen Drugs 0.000 claims abstract description 42
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims abstract description 33
- 239000008103 glucose Substances 0.000 claims abstract description 31
- 238000005259 measurement Methods 0.000 claims abstract description 30
- 239000002002 slurry Substances 0.000 claims abstract description 18
- 108090000790 Enzymes Proteins 0.000 claims abstract description 16
- 102000004190 Enzymes Human genes 0.000 claims abstract description 16
- JVTAAEKCZFNVCJ-UHFFFAOYSA-M Lactate Chemical compound CC(O)C([O-])=O JVTAAEKCZFNVCJ-UHFFFAOYSA-M 0.000 claims abstract description 16
- 230000003301 hydrolyzing effect Effects 0.000 claims abstract description 9
- 238000003307 slaughter Methods 0.000 claims abstract description 9
- 230000007062 hydrolysis Effects 0.000 claims abstract description 5
- 238000006460 hydrolysis reaction Methods 0.000 claims abstract description 5
- 239000007864 aqueous solution Substances 0.000 claims abstract description 3
- 235000013372 meat Nutrition 0.000 claims description 26
- 210000001519 tissue Anatomy 0.000 claims description 23
- 210000003205 muscle Anatomy 0.000 claims description 18
- 108010073178 Glucan 1,4-alpha-Glucosidase Proteins 0.000 claims description 15
- 229940088598 enzyme Drugs 0.000 claims description 15
- 239000000243 solution Substances 0.000 claims description 7
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 claims description 6
- 108010015776 Glucose oxidase Proteins 0.000 claims description 4
- 239000004366 Glucose oxidase Substances 0.000 claims description 4
- 108010073450 Lactate 2-monooxygenase Proteins 0.000 claims description 4
- 229940116332 glucose oxidase Drugs 0.000 claims description 4
- 235000019420 glucose oxidase Nutrition 0.000 claims description 4
- 239000000843 powder Substances 0.000 claims description 4
- 229930027945 nicotinamide-adenine dinucleotide Natural products 0.000 claims description 3
- BOPGDPNILDQYTO-NNYOXOHSSA-N nicotinamide-adenine dinucleotide Chemical compound C1=CCC(C(=O)N)=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OC[C@@H]2[C@H]([C@@H](O)[C@@H](O2)N2C3=NC=NC(N)=C3N=C2)O)O1 BOPGDPNILDQYTO-NNYOXOHSSA-N 0.000 claims description 3
- 102100024295 Maltase-glucoamylase Human genes 0.000 claims description 2
- 108010028144 alpha-Glucosidases Proteins 0.000 claims description 2
- 230000015572 biosynthetic process Effects 0.000 claims description 2
- 239000003795 chemical substances by application Substances 0.000 claims description 2
- 239000006194 liquid suspension Substances 0.000 claims description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 2
- 239000003125 aqueous solvent Substances 0.000 claims 2
- 102000004139 alpha-Amylases Human genes 0.000 claims 1
- 108090000637 alpha-Amylases Proteins 0.000 claims 1
- 229940024171 alpha-amylase Drugs 0.000 claims 1
- 238000003556 assay Methods 0.000 abstract description 2
- 238000012360 testing method Methods 0.000 description 9
- 239000008351 acetate buffer Substances 0.000 description 7
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 6
- 238000002474 experimental method Methods 0.000 description 5
- 238000007792 addition Methods 0.000 description 4
- 239000007788 liquid Substances 0.000 description 4
- VLTRZXGMWDSKGL-UHFFFAOYSA-N perchloric acid Chemical compound OCl(=O)(=O)=O VLTRZXGMWDSKGL-UHFFFAOYSA-N 0.000 description 4
- ZCYVEMRRCGMTRW-UHFFFAOYSA-N 7553-56-2 Chemical compound [I] ZCYVEMRRCGMTRW-UHFFFAOYSA-N 0.000 description 3
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 description 3
- 239000008280 blood Substances 0.000 description 3
- 210000004369 blood Anatomy 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- 239000011630 iodine Substances 0.000 description 3
- 229910052740 iodine Inorganic materials 0.000 description 3
- 229910052757 nitrogen Inorganic materials 0.000 description 3
- 239000002904 solvent Substances 0.000 description 3
- QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 description 2
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- WQZGKKKJIJFFOK-DVKNGEFBSA-N alpha-D-glucose Chemical compound OC[C@H]1O[C@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-DVKNGEFBSA-N 0.000 description 2
- 235000015278 beef Nutrition 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 239000002207 metabolite Substances 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 239000004382 Amylase Substances 0.000 description 1
- 241000228245 Aspergillus niger Species 0.000 description 1
- 241000283690 Bos taurus Species 0.000 description 1
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 1
- 238000013494 PH determination Methods 0.000 description 1
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 1
- 229910000831 Steel Inorganic materials 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 235000019270 ammonium chloride Nutrition 0.000 description 1
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 1
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 1
- 239000001166 ammonium sulphate Substances 0.000 description 1
- 235000011130 ammonium sulphate Nutrition 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 239000001110 calcium chloride Substances 0.000 description 1
- 229910001628 calcium chloride Inorganic materials 0.000 description 1
- 244000309466 calf Species 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007717 exclusion Effects 0.000 description 1
- ZZUFCTLCJUWOSV-UHFFFAOYSA-N furosemide Chemical compound C1=C(Cl)C(S(=O)(=O)N)=CC(C(O)=O)=C1NCC1=CC=CO1 ZZUFCTLCJUWOSV-UHFFFAOYSA-N 0.000 description 1
- 230000009229 glucose formation Effects 0.000 description 1
- 230000034659 glycolysis Effects 0.000 description 1
- 238000000265 homogenisation Methods 0.000 description 1
- XMBWDFGMSWQBCA-UHFFFAOYSA-N hydrogen iodide Chemical compound I XMBWDFGMSWQBCA-UHFFFAOYSA-N 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 238000006317 isomerization reaction Methods 0.000 description 1
- 239000003041 laboratory chemical Substances 0.000 description 1
- 239000006193 liquid solution Substances 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 238000000691 measurement method Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- 239000002985 plastic film Substances 0.000 description 1
- 229920006255 plastic film Polymers 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000010008 shearing Methods 0.000 description 1
- 239000001632 sodium acetate Substances 0.000 description 1
- 235000017281 sodium acetate Nutrition 0.000 description 1
- 239000007974 sodium acetate buffer Substances 0.000 description 1
- 229910001220 stainless steel Inorganic materials 0.000 description 1
- 239000010935 stainless steel Substances 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 239000010959 steel Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- C12Q1/40—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving amylase
Definitions
- the present invention relates to a rapid method for measuring complex carbohydrates
- glycogen in mammalian tissue. More particularly the present invention relates
- glycogen in mammalian tissue.
- the measurement of glycogen includes, for example, the ability to use the results of
- glycogen measurement as a determination of the ultimate pH of meat. This in turn is a direct
- the iodine method The principle of this method is that glycogen will react with a mixture
- glycogen is extracted from the
- glycogen which is reacted with the iodine can also be by liquid nitrogen,
- amyloglucosidase method of Dreiling et al (Meat Science, Vol 20, p. 167) is one
- a muscle sample is homogenised with
- Amyloglucosidase is added, converting the glycogen to glucose, for
- the first part of the method takes approximately 30 minutes at 37°C.
- the method also utilise mild or non-aggressive reagents.
- low glycogen levels in the liver of bobby calves are an
- rapid is used to refer to times of less than
- said complex carbohydrate is glycogen.
- solvent is water.
- said solvent may include at least one agent intended to
- steps (b) and (c) are performed simultaneously.
- steps (c) and (d) are performed simultaneously.
- the hydrolysing enzyme may be any enzyme, or combination of enzymes,
- hydrolysing enzyme is selected from the group: amyloglucosidase; -amylase;
- ⁇ -glucosidase ⁇ -glucosidase
- step (c) measuring complex carbohydrates in mammalian tissue, substantially as described above, wherein the amyloglucosidase added in step (c) is in a form selected from: a powder; a liquid
- said method further includes a step (e): measuring the
- steps (d) and (e) are performed simultaneously.
- steps (c), (d) and (e) are performed simultaneously.
- the method is conducted at approximately room temperature, although it is not
- concentration of glucose is achieved by the construction of sensors incorporating said
- hydrolysing enzyme and glucose oxidase hydrolysing enzyme and glucose oxidase.
- lactate as catalysed by lactate oxidase.
- This latter compound can be incorporated into any combination
- Figure 1 is a graph of glucose concentration and glucometer reading of a glucose
- Figure 2 is a graph of the glucometer reading for glucose and concentration of glucose added to meat slurry samples
- Figure 3 is a graph of the kinetics of glucose formation from glycogen in acetate buffer
- Figure 4 is a graph of glucometer readings and the concentration of glucose added to
- Figure 5 is a graph of glucose value and glycogen added to a post rigor meat slurry, at
- Figure 6 is a graph of muscle glycogen concentration in post slaughter samples, using
- Figure 7 is a graph showing the kinetics of glycogen loss, pH fall and lactate increase
- Figure 8 is a graph of the repeatability of glycogen determination by the method of the
- solution can be obtained by the use of 25 mg of powder, dissolved in 5 ml of 0.2M sodium acetate at a pH of 4.5. ⁇ -D-Glucose of a standard analytical grade was used.
- Glucose measurement was made with an Esprit glucometer (Bayer). Test sensors used in the
- Esprit were from Bayer New Zealand Limited. These were used for one reading only then
- the meat samples tested were obtained from the longissimus lumborum muscle of a beef
- test medium for all experiments was 0.2M sodium acetate buffer at a pH of 4.4 and at
- Polytron shearing head This was usually set at 25,000 ⁇ m. Alternatively a lower speed
- the homogeniser may be used, if so desired.
- the homogeniser may be a stainless steel paddle ⁇
- lactate concentration was also measured. At various times after
- amyloglucosidase was centrifuged in a micro-centrifuge (at 10, 000 ⁇ m for 30 seconds).
- the density of meat is about 1 g/ml.
- Example 1 The method of Example 1 was repeated over a range of samples and concentrations in which
- glycogen between 0 - 14 mg were added to 5 ml of acetate buffer and
- Meat samples ranged from between 0.97 - 1.07 g in six tests. The results are as
- gluteus maxims muscle sample contained 13.1 mg/g at 2.3 hours post slaughter
- This muscle is frequently used as an indicator of high pH condition. Lactate concentrations were also measured.
- NADH-linked method rather than the use of a sensor based on lactate oxidase.
- glycogen were added to three replicate rigor meat samples weighing 1.0 +/- 0.05 g.
- tissue samples may be equally treated in like manner to produce glycogen
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| NZ33727699 | 1999-08-17 | ||
| NZ33727699 | 1999-08-17 | ||
| PCT/NZ2000/000159 WO2001012844A1 (en) | 1999-08-17 | 2000-08-17 | Rapid method for measuring complex carbohydrates in mammalian tissue |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| EP1210451A1 true EP1210451A1 (de) | 2002-06-05 |
| EP1210451A4 EP1210451A4 (de) | 2004-08-25 |
Family
ID=19927450
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| EP00955193A Withdrawn EP1210451A4 (de) | 1999-08-17 | 2000-08-17 | Schnellverfahren zum messen komplexer kohlenhydrate in säugergeweben |
Country Status (6)
| Country | Link |
|---|---|
| EP (1) | EP1210451A4 (de) |
| AR (1) | AR025303A1 (de) |
| AU (1) | AU779415C (de) |
| BR (1) | BR0013395A (de) |
| CA (1) | CA2382155A1 (de) |
| WO (1) | WO2001012844A1 (de) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB0218868D0 (en) | 2002-08-14 | 2002-09-25 | Nasir Muhammed A | Improved airway management device |
| GB2521375C (en) | 2013-12-17 | 2021-09-29 | Aslam Nasir Muhammed | Intubating Airway Device |
| USD842456S1 (en) | 2015-12-15 | 2019-03-05 | Intersurgical Ag | Airway device |
| USD1051359S1 (en) | 2015-06-15 | 2024-11-12 | Intersurgical Ag | Airway device |
| GB201720733D0 (en) | 2017-12-13 | 2018-01-24 | Ashkal Development Ltd | Airway device |
| USD1025348S1 (en) | 2020-04-16 | 2024-04-30 | Intersurgical Ag | Airway device |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0086108A2 (de) * | 1982-02-08 | 1983-08-17 | Children's Hospital Medical Center | Verfahren zur Milchsäuremessung in einer Flüssigkeit |
| US5262305A (en) * | 1991-03-04 | 1993-11-16 | E. Heller & Company | Interferant eliminating biosensors |
-
2000
- 2000-08-17 AU AU67425/00A patent/AU779415C/en not_active Ceased
- 2000-08-17 BR BR0013395-7A patent/BR0013395A/pt not_active IP Right Cessation
- 2000-08-17 CA CA002382155A patent/CA2382155A1/en not_active Abandoned
- 2000-08-17 AR ARP000104247 patent/AR025303A1/es unknown
- 2000-08-17 EP EP00955193A patent/EP1210451A4/de not_active Withdrawn
- 2000-08-17 WO PCT/NZ2000/000159 patent/WO2001012844A1/en not_active Application Discontinuation
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0086108A2 (de) * | 1982-02-08 | 1983-08-17 | Children's Hospital Medical Center | Verfahren zur Milchsäuremessung in einer Flüssigkeit |
| US5262305A (en) * | 1991-03-04 | 1993-11-16 | E. Heller & Company | Interferant eliminating biosensors |
Non-Patent Citations (5)
| Title |
|---|
| BURTON SHELLEY A ET AL: "Evaluation of a glucose oxidase/peroxidase method for indirect measurement of glycogen content in marine mussels (Mytilus edulis)" JOURNAL OF SHELLFISH RESEARCH, vol. 16, no. 2, December 1997 (1997-12), pages 435-439, XP001182147 ISSN: 0730-8000 * |
| BURTON SHELLEY A ET AL: "Evaluation of a glucose oxidase/peroxidase method for indirect measurement of glycogen content in oysters (Crassostrea virginica)" JOURNAL OF SHELLFISH RESEARCH, vol. 19, no. 2, December 2000 (2000-12), pages 841-844, XP001181700 ISSN: 0730-8000 * |
| CARR R S ET AL: "Quantitative semi-automated enzymatic assay for tissue glycogen." COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY. B, COMPARATIVE BIOCHEMISTRY. ENGLAND 1984, vol. 77, no. 3, 1984, pages 447-449, XP002286075 ISSN: 0305-0491 * |
| See also references of WO0112844A1 * |
| VOET AND VOET: "Biochemie" 1992 , VCH , WEINHEIM XP002286178 * page 480, column 1, paragraph 1 * * |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2001012844A1 (en) | 2001-02-22 |
| AU6742500A (en) | 2001-03-13 |
| BR0013395A (pt) | 2002-07-09 |
| CA2382155A1 (en) | 2001-02-22 |
| EP1210451A4 (de) | 2004-08-25 |
| AR025303A1 (es) | 2002-11-20 |
| AU779415B2 (en) | 2005-01-20 |
| AU779415C (en) | 2006-02-09 |
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Legal Events
| Date | Code | Title | Description |
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| PUAI | Public reference made under article 153(3) epc to a published international application that has entered the european phase |
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| A4 | Supplementary search report drawn up and despatched |
Effective date: 20040712 |
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| STAA | Information on the status of an ep patent application or granted ep patent |
Free format text: STATUS: THE APPLICATION IS DEEMED TO BE WITHDRAWN |
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| 18D | Application deemed to be withdrawn |
Effective date: 20070301 |