EP1187934A1

EP1187934A1 - Cell-based assay for screening cox-2 inhibitors

Info

Publication number: EP1187934A1
Application number: EP00941446A
Authority: EP
Inventors: E. Premkumar Reddy; M.V. Ramana Reddy; N. Dhanasekaran
Original assignee: Temple Univ School of Medicine
Current assignee: Temple Univ School of Medicine
Priority date: 1999-06-16
Filing date: 2000-06-15
Publication date: 2002-03-20
Also published as: AU5615200A; CA2374676A1; WO2000077245A1; EP1187934A4; WO2000077245A9

Abstract

A cell-based assay is provided for screening substances for the capacity to inhibit COX-2 activity. The assay utilizes as a screening reagent a cell line which has been engineered to achieve constitutive or inducible expression of COX-2. In one embodiment, the cells express a GTPase-deficient, constitutively-activated mutant form of the α-subunit of protein G12 which induces the production of arachidonic acid and COX-2. Decreased cell proliferation in the presence of test substance indicates that the substance has COX-2 inhibitory activity.

Description

CELL-BASED ASSAY FOR SCREENING COX-2 INHIBITORS

Cross-Reference to Related Application

The benefit of the filing date of U.S. provisional patent application Ser. No. 60/139,569 filed June 16, 1999 is hereby claimed pursuant to 35 U.S.C. 119(e). The entire disclosure of the aforesaid provisional application is incorporated herein by reference.

Reference to Government Grant

The invention described herein was supported in part by National Institutes of Health grant GM49897. The U.S. government has certain rights in the invention.

Field of the Invention

The invention relates to a method forthe identification of compounds having biological activity. In particular, the invention relates to the identification of compounds which inhibit the activity of cycloxygenase-2.

Background of the Invention

Heterotrimeric G Protein

Heterotrimeric guanine nucleotide-binding regulatory proteins

("heterotrimeric G proteins") that transduce signals from cell surface receptors to intracellular effectors are composed of , β and γ subunits.

Gα subunits are grouped based on amino acid homology into four subfamilies: G_s, G„ G_q and G₁₂ (Strathman and Simon, Proc. Natl. Acad. Sci. (USA) 1991 ; 88:5582-5582). The α subunits possess intrinsic GTPase activity and belong to a much larger group of GTPases which share structural elements. In all of these GTPases, a cycle of guanine nucleotide exchange and hydrolysis enables the protein to exist in two distinct states. The cycle allows G proteins to transiently relay signals from cell-surface receptors to intracellular effectors. Upon interaction with the appropriate agonist, the receptor serves to accelerate the exchange of GDP for GTP on the G protein α subunit. The exchange is believed to be accompanied by dissociation of the α and β-γ subunits, allowing α (and in some cases β- γ) to interact with effectors. The intrinsic GTPase activity terminates the signal, returning the α subunit to its basal GDP-bound state. Studies have suggested that the G₁₂ members α₁₂ and α₁₃ regulate signaling pathways involved in controlling cell growth and differentiation. See Vara Prasad et al., J. Biol. Chem. 1995, 270:18655-18659. The full-length cDNAs encoding mouse Gα12 and Gα13, and the encoded translation products, are disclosed in Strathman and Simon, supra, the entire disclosure of which is incorporated herein by reference.

The sequences have been deposited in the GenBank data base by the authors, accession nos. M63659 (Gα12) and M63660 (Gα13). Those GenBank records are incorporated herein by reference. The human Gα₁₂ cDNA has also been cloned (Chan etal., Mol. Cell. Biol. 1993; 13:762-768, incorporated herein by reference).

When constitutively activated, GTP-binding proteins can induce neoplastic transformation. See Xu et al., Proc. Natl. Acad. Sci. (USA) 1993: 90:6741-6745, the entire disclosure of which is incorporated herein by reference. Overexpression of wild-type Gα₁₂ in NIH 3T3 cells is weakly transforming; a GTPase-deficient mutant of Gα₁₂ (Gα₁₂Q229L) behaves as a potent oncogene and is highly transforming in NIH 3T3 cells (Xu et al., supra). It has been found that Gα₁₂-transfected cells exhibit a remarkably increased level of arachidonic acid in response to serum, and this effect is observed in cells transfected with either wild-type or activated mutant Gα₁₂ (Xu et al. , supra).

The Gα₁₂Q229L mutant results from insertion of a leucine residue at position 229 in lieu of glutamine in the wild-type Gα₁₂ protein. The mutation occurs in a highly conserved region in G proteins which is involved in binding and GTPase activity. The Q229L mutation results in a GTPase- deficient form of Gα₁₂. The mutation blocks GTPase activity so that the α subunit binds GTP and is constitutively active (Xu et al., supra; Dhanasekaran et ai, J. Biol. Chem. 1993, 269:11802-11806; Jian et al., FEBS Lett. 1993; 330:319-322; Vara Prasad et al,, Oncogene 1994, 9:2425-2429; Voyno-Yasenetskaya et al., Oncogene 1994; 9:2559-2565).

COX-2

Cyclooxygenase-1 (COX-1 ) and cyclooxygenase-2 (COX-2) are the enzymes which convert arachidonic acid into prostaglandins. COX-1 is ubiquitously expressed and involved in cellular "housekeeping" functions of various tissues and organs. In contrast, COX-2 expression is rapidly induced in diverse cell types by different growth factors, mitogens, tumor promoters, and physiological stress stimuli. Transcriptional induction of COX-2 has been shown to be involved in different pathological conditions such as inflammation, pain, and fever. It has also been shown that the anti-inflammatory effects of aspirin and ibuprofen is through their inhibitory effect, albeit non-specific inhibitory effect, on COX-2. Furthermore, persistent activation of COX-2 has been shown to be associated with oncogenesis as well as the invasive potential of tumor cells.

Inhibitors of COX-2 are useful as therapeutics. See, for example, the discussion of the advantages of selective COX-2 inhibition set forth in U.S. Pat. 5,604,253.

What is needed is a simple, sensitive and rapid screening method for determining the COX-2 inhibitory activity of therapeutic candidates. Summary of the Invention

It is an object of the invention to provide a screening method to determine the COX-2 inhibitory activity of chemical compounds.

It is an object of the invention to provide a screening method for COX-2 inhibition which relies on cells which respond to the presence of COX-2 inhibitor by reducing proliferation in comparison to cells of the same type which are not contacted with COX-2 inhibitor.

It is an object of the invention to provide a screening method for

COX-2 inhibition which relies on cells which respond to the presence of COX-2 inhibitor by increasing production of one or more prostaglandins in comparison to cells of the same type which are not contacted with COX-2 inhibitor.

It is an object of the invention to provide a screening method for COX-2 inhibition which relies on cells which respond to the presence of COX-2 inhibitor by accumulating arachidonic acid in comparison to cells of the same type which are not contacted with COX-2 inhibitor.

A method for screening a test substance for COX-2 inhibitory activity is provided. The test substance is contacted with indicator cells which constitutively or inducibly express endogenous COX-2. The level of proliferation of the indicator cells is determined in the presence and absence of the test substance. A decreased level of proliferation of the indicator cells in the presence of the test substance indicates that the test substance has COX-2 inhibitory activity.

According to another method for screening a test substance for COX-2 inhibitory activity, the test substance is contacted with indicator cells which constitutively or inducibly express COX-2, and the level of one or more prostaglandins produced by the indicator cells is determined in the presence or absence of the test substance. A decreased level of one or more prostaglandins produced by the indicator cells in the presence of a test substance indicates that the test substance has COX-2 inhibitory activity.

According to another method for screening a test substance for COX-2 inhibitory activity, the test substance is contacted with indicator cells which constitutively or inducibly express COX-2, and the level of arachidonic acid provided by the indicator cells is determined in the presence or absence of the test substance. An increased level of arachidonic acid provided by the indicator cells in the presence of a test substance indicates that the test substance has COX-2 inhibitory activity. According to preferred embodiments of the invention, indicator cells constitutively or inducibly express endogenous COX-2. According to other preferred embodiments of the invention, the indicator cells express a GTPase-deficient mutant form of the α-subunit of protein G12. The mutant α-subunit has the capacity to induce the production of arachidonic acid and COX-2 in the indicator cells. According to one embodiment of the invention, the G12 protein c - subunit mutant comprises the Q229L mutation.

The level of indicator cell proliferation is conveniently determined by an assay for DNA synthesis by the indicator cells. According to one embodiment, the DNA synthesis assay comprises assaying tritium-labeled thymidine uptake by the indicator cells.

The level of indicator cell production of one or more prostaglandins is conveniently determined with resort to any assay which is capable of quantifying, at least in relative terms as compared to control cells, the level of production of such prostaglandins. According to a preferred embodiment, the prostaglandin assay method comprises an immunoassay. According to a more preferred embodiment, the immunoassay comprises a competitive immunoassay for a prostaglandin.

The level of arachidonic acid in the indicator cells, the components thereof, or released into the medium surrounding the cells, is conveniently determined with resort to any assay which is capable of quantifying, at least in relative terms as compared to control cells, the level of arachidonic acid. One such method is a radiolabeling assay. According to one embodiment, the assay comprises assaying release of tritiated arachidonic acid by the indicator cells.

Description of the Figures Fig. 1 is a schematic representation of the mechanism by which

COX-2 plays a role in proliferation of cells expressing the GTPase-deficient Gα₁₂QL mutant.

Fig. 2A is a Northern blot of analysis of RNA from IPTG-inducible

Gα₁₂QL-NIH3T3 cells stimulated with 1 mM IPTG for 0, 0.5, 1 , 3 and 6 hours. Cells were probed with DNA encoding Gα₁₂QL, COX-2 or GAPDH.

Fig. 2B is similar to Fig. 2A, except that the analysis was carried out on control cells. The control cells were NIH3T3 cells transfected with vector lacking the Gα₁₂QL insert.

Fig. 3 is Western blot analysis of cell lysates probed for COX-2 expression. The lysates were prepared from Gα₁₂QL-NIH3T3 cells (lanes 3 and 6), control NIH3T3 cells (lanes 1 and 4) and Gα₁₂WT-NIH3T3 cells expressing wild type Gα₁₂ (lanes 2 and 5).

Fig. 4 is a graph of [³H]-thymidine incorporation as an index of DNA synthesis in NIH3T3 cells transfected with Gα₁₂QL DNA (Gα₁₂QL-NIH3T3) versus control NIH3T3 cells transfected with vector alone (pcDNA3-

NIH3T3). Values represent the mean ±S.E. from a triplicate determination.

Fig. 5 is a graph of prostaglandin release from NIH3T3 cells as a function of stable transfection with Gα₁₂QL expression vectors and pcDNA3. Values represent the mean ±S.E. from a triplicate determination. Fig. 6A is a graph of [³H]-arachidonic acid release from Gα₁₂QL expression vector or pcDNA3 transfected NIH3T3 cells. Quiescent, stably transfected cells were stimulated with serum and [³H]-arachidonic acid release was followed as a function of time. Values represent the mean

±S.E. from a triplicate determination. Fig.6B is a graph of [³H]-arachidonic acid release from pcDNA3, ras, Gα₁₂QL, and Gα₁₃QL expression vector stably transfected NIH3T3 cells. Quiescent, transfected cells were stimulated with serum and [³H]- arachidonic acid release was measured after 10 minutes. Values represent the mean ±S.E. from a quadruplicate determination.

Detailed Description of the Invention

A cell based bioassay system is provided for screening compounds for COX-2 inhibitory activity by assaying the growth-inhibitory property of the compounds when exposed to COX-2 growth-driven target cells. The target cells are cells which are capable of growing in culture but which have been engineered to constitutively or inducibly express COX-2, preferably endogenous COX-2.

By "expression" with respect to COX-2 is meant the production of active COX-2 enzyme by the cell.

By "endogenous" with respect to COX-2 is meant DNA sequences corresponding to the native, COX-2 gene locus, its variants, or derivatives present in the cells of the organism from which a given indicator cell line was derived.

By "constitutively express" with respect to COX-2 is meant that biologically active COX-2 protein is present continually in a cell and does not appear to be subject to quantitative regulation.

By "inducibly express" with respect to COX-2 is meant that the COX- 2 gene is activated in response to a specific stimulus, such as the presence of a specific small molecule inducer, to produce biologically active COX-2 protein. The gene is not activated, or activated only at a much lower level, in the absence of the specific stimulus. Inducible expression of the COX-2 gene may be controlled directly to achieve inducible expression, such as by an inducible promoter operably linked to a COX-2 gene in an expression construct. Alternatively, inducible expression of the COX-2 gene may be controlled indirectly, such as by an inducible promoter operably linked to a second gene other than the COX-2 gene. The inducible expression of the second gene in turn governs the expression of COX-2.

COX-2 is activated by several different pathways in the cell (Vane et al., Annu. Rev. Pharmacol. Toxicol. 1998; 38:97-120, incorporated herein by reference). Each pathway is a target for manipulation of COX-2 gene expression to achieve either constitutive or inducible expression of that gene. Cell lines which express endogenous COX-2 and produce prostaglandins may be appropriate indicator cells for screening for COX-2 inhibitors. An example of such a cell line is the MDA-MB-231 human breast cancer cell line (ATCC number HTB-26; Liu and Rose, Cancer Res. 1996; 56:5125-5127, incorporated herein by reference).

According to one embodiment, the indicator cells express a GTPase- deficient, constitutively-activated mutant form of the α-subunit of the heterotrimeric protein G12 ("Gα₁₂ mutant"). By "GTPase-deficient" is meant that the molecule lacks intrinsic GTPase activity. Hence, the Gα₁₂ mutant is constitutively active. By "constitutively activate" is meant that the activity of the protein is present essentially continually in the cell and is not subject to quantitative regulation. The Gα₁₂ mutant has the property of inducing the production of arachidonic acid. Arachidonic acid is a substrate of COX-2. COX-2 converts arachidonic acid into prostaglandins. The latter stimulate cell proliferation. The Gα₁₂ mutant also has the property of inducing the transcription of COX-2, which leads to the constitutive expression of COX-2 in cells engineered to express the mutant.

Contact of indicator cells with an inhibitor of COX-2 has the effect of inhibiting production of prostaglandins, and thus cell proliferation. At the same time, the prostaglandin precursor arachidonic acid will accumulate in the cell or culture medium due to the blockage of COX-2-mediated conversion of arachidonic acid to prostaglandin.

One such Gα₁₂ mutant is the protein Gα₁₂Q229L ("Gα₁₂QL") described by Xu et al., supra. The starting material for the Gα₁₂QL (also referred to herein as "Q229L") mutant is the full-length murine wild-type Gα₁₂ cDNA disclosed by Strathman and Simon, Proc. Natl. Acad. Sci. (USA) 1991 ; 88:5582-5582 or GenBank accession record M63659. The mutant is generated by the method described by Xu et al., supra, utilizing site-directed mutagenesis of double-stranded DNA by overlap extension using PCR methodology. The mutagenic oligonucleotides have the following sequences, wherein the modified nucleotides are underlined: 5'-

TGGGCGGCCIGAGGTCAC-3 ' (SEQ I D NO: 1 ) and 5'-

GTGACCTCAGGCCGCCCA-3' (SEQ ID NO:2).

Cells engineered to express Gα₁₂QL are driven to proliferation through an autocrine loop shown in Fig. 1. COX-2 inhibitors can arrest cell proliferation by disrupting this autocrine loop. Such an inhibitory effect can be easily quantified by monitoring the Gα₁₂QL-stimulated synthesis of DNA.

A putative COX-2 inhibitor can thus be easily and rapidly screened for

COX-2 inhibitory activity.

Prior to the present invention, it was not known that the Q229L mutation induces COX-2 transcription in cells. It has been unexpectedly found that Gα₁₂QL-transformed cells constitutively express COX-2, thereby permitting their use as reagents for screening putative COX-2 inhibitors. According to the invention, a host cell line capable of growth in culture, preferably a fibroblast line (e.g. NIH 3T3), is transfected with a construct which will drive Gα₁₂QL DNA expression in the host cell line. The construct contains Gα₁₂QL DNA and appropriate control elements. The construct may contain a non-inducible promoter, or an inducible promoter, e.g., an isopropyl-β-D-1-thiogalactopyranoside (IPTG)-inducible promoter.

Methods for establishing cell lines that expresses Gα₁₂QL under control of an IPTG promoter are described by Vara Prasad et al., J. Biol. Chem.

1995, 270:18655-18659, the entire disclosure of which is incorporated herein by reference.

Cells of the transfected cell line which produce arachidonic acid and

COX-2 are then used as indicator cells for testing the COX-2 inhibitory activity of test substances. The test substance may comprise a pure chemical compound or mixture of chemical compounds. Typically, the test substance will be an essentially pure chemical compound which may be contained in a suitable carrier for administration to the indicator cell culture. An appropriate concentration of test substance is introduced into the cell culture. An appropriate concentration can be determined by trial and error, without undue experimentation. The concentration of test substance may range, for example, from about 0.1 to about 100 μM, more typically from about 1 to about 20 μM, most typically from about 1 to about 10 μM.

According to one embodiment of the invention, the level of proliferation of the indicator cells with and without the test substance present in the cell culture is then determined. The test substance is contacted with the indicator cells under conditions which favor the proliferation of those cells. Methods for culturing cells under conditions of proliferation are well-known to those skilled in the art. Typically, conditions favoring cell proliferation in culture comprise culturing the cells in the presence of a growth medium, e.g., a growth medium comprising a serum such as fetal bovine serum (FBS). Assays for determining the level of proliferation of cells in culture are well-known to those skilled in the art. In addition to determining actual cell number, several indirect methods for assessing cell proliferation are known to those skilled in the art. According to one preferred embodiment of a proliferation assay, proliferation is assessed by monitoring DNA synthesis. Proliferating cells actively synthesize DNA to support mitosis. DNA synthesis may be conveniently monitored through the cell's uptake of detectably labeled DNA precursors. Such labeled precursors include, for example, chemically- labeled and radiolabeled precursors. One chemically labeled precursor is bromodeoxyuridine. Bromodeoxyuridine incorporation can be detected by bromodeoxyuridine-antibodies in an enzyme-linked immunosorbent assay using fixed microcultures (Muir et al., Analytical Biochemistry 1990; 185:377-382). The preferred radiolabeled precursor is tritium-labeled thymidine (³H-thymidine). Other chemically labeled and radiolabeled DNA precursors suitable for use in cell proliferation assays are known to those skilled in the art. The capacity of the test substance to inhibit cell proliferation is a measure of the potency of the substance as an inhibitor of COX-2 activity.

According to another embodiment of the invention, the level of one or more prostaglandins produced by the indicator cells, with and without the test substance present in the cell culture, be can determined. Such prostaglandins may include for example PGE₂, PGF_2α, PGD₂, their stabilized chemical derivatives and the like. In samples were the chemical environment promotes the rapid turnover of prostaglandins such prostaglandins may be converted to stable derivatives prior to detection. Such stabilized derivatives include for example bicyclo prostaglandin E2, 8-isoprostane, and prostaglandin D2 methoxime which correspond to PGE₂, PGF_2α! and PGD₂. A decrease in production of prostaglandins by the indicator cells in the presence of the test substances indicates that the test substance has COX-2 inhibitory activity. Assays for determining the level of prostaglandins produced by cells in culture are well-known to those skilled in the art. Such methods include, for example, chromatography- based techniques, receptor based assays or immunoassays for the prostaglandin(s) of interest. Receptor based prostaglandins assays may be based on the methods of Hanasaki et al. or Balapure et al. for example.

Hanasaki et ai, J. Biol. Chem. 1990;265:4871-4875 and Balapure et al.,

Biochem. Pharmacol. 1989;38:2375-2381. Prostaglandin production may be measured by the various methods in the media surrounding cells, in the cells themselves, or in a component of the cells. Components of the cells include for example organelles, membranes, proteins, cell lysates, cell pellets, cell supematants and the like.

For example, prostaglandin production in radiolabeled cells may be assayed by a thin layer chromatography (TLC) as follows. Radiolabeled prostaglandins are extracted from cells, their components, or the surrounding media with an appropriate solvent such as ethyl acetate. The solvent-extracted radiolabeled prostaglandins are then spotted on a TLC plate and developed under suitable conditions in an appropriate mobile phase. Radiolabeled prostaglandins may be detected by any suitable means such as phosporimaging, fluorography, scintillation counting, auto radiography and the like. Alternatives to TLC chromatography include any chromatography technique capable of discerning the relative amounts of prostaglandins produced by unlabeled or radiolabeled cells.

According to one preferred embodiment of the invention, prostaglandin production by the indicator cells is measured by a competitive immunoassay. In such an assay, a primary antibody specific to one or more prostaglandins is incubated with a sample to be analyzed followed by incubation of the sample with an immobilized secondary antibody specific to the primary antibody. A labeled prostaglandin is then incubated with the sample, and the labeled prostaglandin is allowed to compete with non-conjugated prostaglandins in the sample for prostaglandin binding sites on the primary antibody. The competing labeled prostaglandin may be labeled by any suitable means including radiolabeling, conjugation with a peroxidase or other easily detected polypeptide, chromophore labeling, orfluorophore labeling. Techniques for labeling prostaglandins are well known to those skilled in the art. The level of competing, labeled prostaglandin bound after washing is inversely related to the amount of prostaglandin present in the sample analyzed. (Shaw and Ramwell, Methods of Biochem. Analysis 1969; 17:325-371 Green et al., Advances in Prostaglandin and Thromboxane Res. 1978 5:15-38; Powell, Prostaglandins 1980; 20:947-957; Kelly et al. Prostaglandins, Leukotrienes and Essential Fatty Acids 1989; 37:187-191 Granstrom and Kindhal, Advances in Prostaglandin and Thromboxane Res 1980; 5:119-210; Morris et al., Prostaglandins 1981 ; 21 :771-778 Granstrom and Samuelsson, Advances in Prostaglandin and Thromboxane Res. 1978; 5:1-13). A competitive prostaglandin assay permits rapid, high throughput screening for COX-2 inhibitors using the indicator cells of the invention. As an alternative to measuring prostaglandin production by the indicator cells, the level of arachidonic acid provided by the indicator cells is determined, with and without the test substance present in the cell culture. By a "level" of arachidonic acid which is "provided" by the indicator cells, is meant the relative amount or concentration of arachidonic acid in the cells or component thereof, or in the culture medium. Arachidonic acid is readily converted to prostaglandins in the presence of functional COX-2. An increase in the level of arachidonic acid by the indicator cells in the presence of the test substance indicates that the test substance has COX- 2 inhibitory activity. Assays for determining the level of arachidonic acid produced by cells in culture are well-known to those skilled in the art. Arachidonic acid production may be measured in the media surrounding cells, in the cells themselves or in components of the cells. According to one preferred embodiment of the invention, arachidonic acid release is measured by labeling indicator cells with tritiated arachidonic acid ([³H]- arachidonic acid), washing cells to remove free radiolabeled arachidonic acid, treating the cells with the test substances and after a time measuring by scintillation counting the radiolabeled arachidonic acid present in the media surrounding the cells. (Xu et al., supra). In the absence of functioning COX-2 the radiolabelled arachadonic acid taken up by the cells is not converted to prostaglandin, but rather is released to the surrounding media.

The inhibitory compounds identified using the invention may be administered to individuals (animals, most particularly mammals including humans) afflicted with any disorder characterized by undesirable prostaglandin production resulting from cyclooxygenase activity, particularly COX-2 activity ("cyclooxygenase-mediated disorder"). In particular, COX-2 inhibitory compounds of the type identified using the invention are believed useful in treating infiamation and inflamation-related disorders, by administering to a subject having or susceptible to such infiamation or inflamation-related disorder and effective amount of an inhibitory compound. Infiamation is associated with a variety of disease conditions. For a list of such disease conditions treatable by cyclooxygenase inhibitors, and COX-2 inhibitors in particular, see U.S. Patents 5,604,253 and 5,908,852, the entire disclosures of which are incorporated herein by reference. Such conditions include, for example, arthritis, including but not limited to rheumatoid arthritis, spondyloarthropathies, gouty arthritis, osteoarthritis, systemic lupus erythematosus and juvenile arthritis. Such conditions further include rheumatic fever, symptoms associated with influenza or other viral infections, common cold, low back and neck pain, dysmenorrhea, headache, toothache, sprains and strains, myositis, neuralgia, synovitis, gout and ankylosing spondylitis, bursitis, and following surgical and dental procedures. COX-2 inhibitory compounds are also believed useful as analgesics for treating or alleviating all forms of pain. The compounds are believed useful in the treatment of other disorders including asthma, bronchitis, tendinitis, bursitis; skin related conditions such as psoriasis, eczema, burns and dermatitis; gastrointestinal conditions such as inflammatory bowel disease, Crohn's disease, gastritis, irritable bowel syndrome and ulcerative colitis and for the prevention of colorectal cancer; the treatment of infiamation in such diseases as vascular diseases, migraine headaches, periarteritis nodosa, thyroiditis, aplastic anemia, Hodgkin's disease, sclerodoma, type I diabetes, myasthenia gravis, sarcoidosis, nephrotic syndrome, Behcet's syndrome, polymyositis, gingivitis, hypersensitivity, conjunctivitis, swelling occurring after injury, myocardial ischemia, and the like. The COX-2 inhibitory compounds identified using the invention are also believed useful as antipyretics forthe treatment of fever.

In addition, COX-2 inhibitors of the type identified using the invention may inhibit cellular neoplastic transformations and metastatic tumor growth and hence can be used in the treatment of cancer. The term "neoplasia" includes neoplasias that produce prostaglandins or express a cyclooxygenase, including both benign and cancerous tumors, growths and polyps. Neoplasias believed treatable with cyclooxygenase inhibitors are discussed in U. S. Pat. 5,972,986, the entire disclosure of which is incorporated herein by reference. The COX-2 inhibitors identified using the invention may be used to inhibit the growth of an established neoplasm, i.e., to induce regression, or to prevent or delay the onset of the neoplasm. According to U.S. Pat. 5,972,986, neoplasias that produce prostaglandins, and which are therefore believed treatable with compounds of the type identified using the invention, include brain cancer, bone cancer, epithelial cell-derived neoplasia (epithelial carcinoma) such as basal cell carcinoma, adenocarcinoma, gastrointestinal cancer such as lip cancer, mouth cancer, esophageal cancer, small bowel cancer and stomach cancer, colon cancer, liver cancer, bladder cancer, pancreas cancer, ovary cancer, cervical cancer, lung cancer, breast cancer and skin cancer, such as squamous cell and basal cell cancers, prostate cancer, renal cell carcinoma, and other known cancers that effect epithelial cells throughout the body.

The COX-2 inhibitory compounds identified by using the invention may also be useful in the treatment of angiogenesis-mediated disorders. Angiogenesis-mediated disorders which may be treatable with cyclooxygenase inhibitors are discussed in U. S. Pat. 6,025,353, the entire disclosure of which is incorporated herein by reference. According to U. S. Pat. 6,025,353, such disorders include, for example, metastasis, corneal graft rejection, ocular neovascularization, retinal neovascularization, diabetic retinopathy, retrolental fibroplasia, neovascular glaucoma, gastric ulcer, infantile hemaginomas, angiofibroma of the nasopharynx, avascular necrosis of bone, and endometriosis.

The practice of the invention is illustrated by the following non- limiting examples. Example 1 Establishing Gα_1?QL Cell Line

A. Transfection of NIH3T3 Cells with Gα^QL Vector (i) Preparation of Gα_1?QL-NIH3T3 The vector Gα₁₂QL-pcDNA3 was prepared by ligating the EcoRI-

Xbal (1.8 kb) fragment from pcDNA1-α₁₂Q229L into the EcoRI-Xbal site of the pcDNA3 vector (Invitrogen, Carlsbad, CA). Actively proliferating NIH 3T3 cells (1.5 x 10⁶ cells/ 100 mm dish) grown in Dulbecco's modified Eagle's medium (DMEM) containing 10% FBS were transfected with 100 ng of the vector by the calcium precipitation method. The procedures for transfection and transformation of NIH 3T3 cells have been previously described (Vara Prasad et ai, Oncogene 1994, 9:2425-2429, and Shore & Reddy, Oncogene 1989, 4:1411-1423, the entire disclosures of which are incorporated herein by reference.) Individual transformed foci were isolated and expanded for further analysis by Northern and Western analyses for Gα₁₂QL expression as well as anchorage-independent growth in soft-agar according to previously published procedures (Vara Prasad et al., 1994, supra).

(ii) Preparation of IPTG-inducible Gα^QL-NIHSTS An NIH3T3 cell line that expresses Gα₁₂QL under an IPTG-inducible promoter (Gα₁₂QL-pOPRSVI-NIH3T3) was established as previously described (Vera Prasad et al., 1995, supra), as follows. Blunt-ended 1.8 kb Hindlll-Xbal fragment of α₁₂Q229L excised from the Gα₁₂QL-pcDNA3 vector was ligated into the blunted Notl site of the pOPRSVI plasmid following published procedures (Sambrook etal. (1989) Molecular Cloning: A Laboratory Manual 2d Ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY). NIH3T3 cell lines expressing inducible α₁₂Q229L were established using the LacSwitch expression vectors (Stratagene). Briefly, NIH3T3 cell were cotransfected with p3'SS plasmid vector expressing the Lac repressor and pOPRSVI-α₁₂Q229L vector by electroporation, as described by Vera Prasad et al., 1995. B. Preparation of COX-2 DNA

Murine COX-2 DNA was cloned using RT-PCR methods from the RNA prepared from NIH 3T3 cells pretreated (6 hrs) with 100 nM PMA using the following primers: 5'CTCTGCGATGCTCTTCCGAG-3' (SEQ ID NO:3) and 5'-GACTTTTACAGCTCAGTTGAACG-3' (SEQ ID NO:4). The amplified 1827 bp PCR-product was sequenced and the comparison with the published sequences (Kujubu etal., J. Biol. Chem. 1991 , 266, 12866- 12872) confirmed the identity of COX-2 cDNA. The COX-2 PCR-product was purified and cloned into pT7Blue TA vector (Novagen, Madison, WI). The clone was used as a source of probes for Northern blot analysis.

C. Confirmation of COX-2 Expression in IPTG-inducible Gα₁₂QL- NIH3T3 Cells

1. Northern Blotting

Expression of Gα₁₂QL was induced in the IPTG-inducibleGα₁₂-QL- NIH3T3 cells and control cells by the addition of 1 mM IPTG. At O, 0.5, 1 , 3, and 6 hours after the addition of IPTG, total RNA was prepared from the cells following the published procedures of Dhanasekaran et al., J. Biol. Chem. 1993, 269:11802-11806, the entire disclosure of which is incorporated herein by reference. Twenty μg of the RNA (for each time point) was resolved in a denaturing 1 % agarose and 2.2 M formaldehyde gel. The RNA was blotted onto a zeta probe-GT membrane (Bio-Rad, CA) and cross-linked to the membrane by UV light. The Hind lll-Xba I fragment (1.2 kbp) of Gα₁₂QL DNA excised from Gα₁₂QL and the Xba l-Bam HI fragment (1.8 kbp) of COX-2 from the COX-2pT7Blue vector were used as probes in Northern blot analyses. The RNA was also probed for GAPDH expression.

The results of the Northern blot analysis are shown in Figs. 2A (Gα₁₂QL-3NIH3T3) and 2B (control cells). The control cells were NIH3T3 cells transfected with "empty" pOPRSVI vector lacking the Gα₁₂QL insert. COX-2 transcription was induced only in Gα₁₂QL-NIH3T3 cells.

2. Western Blotting

The constitutive expression of COX-2 protein was demonstrated by Western blot analysis. Cell lysates for Western blot analysis were prepared from control NIH3T3, NIH3T3 expressing wild type Gα₁₂ (Gα₁₂WT-NIH3T3) and Gα₁₂QL-NIH3T3 cells. The cells grown in 100 mm dishes, washed twice in phosphate-buffered saline (PBS) and lysed with 0.5 ml of RIPA buffer (10 mM NaPO₄ pH 7,0, 150 mM NaCl, 2 mM EDTA, 1% sodium deoxycholate, 1 % NP40, 0.1% SDS, 50 mM NaF, 200 mM Na₃VO₄, 0.1 % β-mercaptoethanol, 1 mM phenylmethanesulfonyl- fluoride (PMSF), 4 μg/ml aprotinin, and 2 μg/ml leupeptin). The soluble proteins were cleared by centrifugation at 15, 000 g for 10 minutes at 4°C. The lysate (50 μg) was resolved by SDS-PAGE and electroblotted onto polyvinylidene fluoride (PVDF) membranes (Millipore, Bedford, MA) in 10 mM 3-cyclohexylamino-1 -propane sulfonic acid (CAPS) buffer containing 10% methanol using a Mini-Protean apparatus (Bio-Rad, CA). The resolved lysates were probed with specific antibodies raised against the C- terminus of COX-2 (Oxford Biomedical Research, Ml).

The results are shown in Fig. 3. COX-2 is constitutively expressed in Gα₁₂QL-NIH3T3 cells (lanes 3 and 6) but not in control NIH3T3 cells (lanes 1 and 4) or Gα₁₂WT-NIH3T3 cells (lanes 2 and 5).

Example 2 Inhibition of Gα,,QL-NIH3T3 Cell Proliferation by COX-2 Inhibitor

Inhibition of Gα₁₂QL-NIH3T3 cell proliferation by a COX-2 inhibitor was determined by monitoring DNA synthesis. [³H]-Thymidine incorporation was used as an index of DNA-synthesis. Control cells (expressing pcDNA3 vector) and Gα₁₂QL-transformed NIH 3T3 cells were plated at 5 x 10⁴ cells per well in a 24-well plate and grown for 24 hours at 37°C in DMEM containing 5 % FBS. The cells were washed and made quiescent by placing them in DMEM (+0.2 % BSA) without serum for an additional 24 hours. Cell growth was reinitiated by the addition of 10 % FBS with or without an experimental COX-2 inhibitor (10 μM). Twelve hours later 1 μCi ofthymidine (20 Ci/nmol) was added to each well and incubated for an additional 4 hours. The cells were washed in PBS and solubilized in 0.1 % SDS. DNA was precipitated with 10 % chilled TCA and the precipitate was collected on GFC filters. The counts in the precipitate were determined by scintillation counting. The results are shown in Fig. 4. The values represent the mean ± the standard error for triplicate determinations.

The addition of 10 μM COX-2 inhibitor did not have any effect on control cells. In contrast, the COX-2 inhibitor specifically inhibited DNA synthesis in Gα₁₂QL-NIH3T3 cells as shown in Fig. 4. These results indicated that the COX-2-inhibitor did not affect normal cells but arrested cell growth in transformed cells dependent on the COX-2 pathway for cell growth and survival. Since Gα₁₂QL-NIH3T3 cells exhibit such a phenotype, the bioassay system based on Gα₁₂QL-NIH3T3 cells may be effectively used to screen putative COX-2 inhibitors.

Example 3 Determination of Prostaglandin Levels as an Assay for COX-2

Activity

Activation of prostaglandin release by NIH3T3 cells in response to Gα₁₂QL expression was determined by monitoring prostaglandin production. Prostaglandin PGE₂ was used as an index of COX-2 mediated prostaglandin production. Control NIH3T3 cells stably transfected with pcDNA3 vector and Gα₁₂QL-transformed NIH3T3 cells were plated at 3 x 10⁶ cells per 100 mm dish and grown overnight at 37°C in DMEM containing 5 % FBS. The cells were washed and made quiescent by placing them in DMEM (+0.2 % BSA) without serum for an additional 24 hours. Cell growth was reinitiated by the addition of 10% FBS. Six hours later the cells were pelleted by centrifugation. The cell pellet was lysed and total cellular PGE₂ production was measured using a competitive enzyme immunoassay (Prostaglandin E₂ EIA (enzymeimmunoassay) Kit, Cayman Chemical Co., Ann Arbor, Ml, USA) as per the manufacturer's protocol. The results are shown in Fig. 5. The values represent the mean + the standard error for triplicate determinations.

Stable expression of Gα₁₂QL dramatically activated prostaglandin PGE₂ production in the indicator cells in contrast to control NIH3T3 cells stably transfected with pcDNA3 as shown in Fig. 5. Activation or inhibition of COX-2 activity in Gα₁₂QL transfected indicator cells can be assessed by determining the levels of prostaglandins produced by such indicator cells. The assay may be used to screen candidate compounds for inhibition of COX-2 activity.

Example 4

Determination of Arachidonic Acid Levels as an Assay for COX-2

Activity

Arachidonic acid release levels can be used as an index of COX-2 activity. Control NIH3T3 cells stably transfected with pcDNA3 vector and Gα₁₂QL-transformed NIH3T3 cells were plated at 2 x 10⁴ cells per well in a 24-well plate and grown for 24 hours at 37 °C in DMEM containing 5 % FBS. Cells were labeled and made quiescent by incubation for 24 hrs with 0.5 ml serum free DMEM containing 10 mM HEPES (ph 7.4), 0.2 % BSA and 0.5 μC\ [³H]-arachidonic acid per well. Cells were then washed three times with PBS to remove free [³H]-arachidonic acid. Cell growth was reinitiated by the addition of FBS to a final concentration of 5 %. [³H]- Arachidonic acid release into the surrounding media was measured at 0, 2, 5, 10, and 20 min after serum stimulation by scintillation counting as per the method of Xu et al., supra. Prior to scintillation counting cell debris in the media was removed by centrifugation at 2000 g. [³H]-arachidonic acid release by non-serum stimulated cells into the surrounding media was measured at 0, 2, 5, 10, and 20 min by scintillation counting. The results are shown in Fig. 6A. The values represent the mean ± the standard error for triplicate determinations. Serum stimulation of Gα₁₂QL transfected cells dramatically increased arachidonic acid release in contrast to non-serum stimulated indicator cells as shown in Fig. 6A. Arachidonic acid release by control NIH3T3 cells stably transfected with pcDNA3 was unaffected by serum stimulation as shown in Fig. 6A. Activation or inhibition of COX-2 activity in Gα₁₂QL transfected indicator cells can be assessed by determining the levels of arachidonic acid produced by such indicator cells. Inhibition of

COX-2 will presumably increase arachidonic acid levels as such inhibition will prevent conversion of arachidonic acid into the various prostaglandins.

Arachidonic acid release levels were also used as an index of COX- 2 activity in NIH3T3 cells stably transfected with pcDNA3, ras, Gα₁₂QL, and Gα₁₃QL expression vectors as shown in Fig. 6B. Cells were plated, labeled with [³H]-arachidonic acid, and [³H]-arachidonic acid release was measured using the methodologies described above. [³H]-arachidonic acid release was measured by scintillation counting 10 min after reinitiation of cell growth by addition of 5% FBS. The results are shown in Fig. 6B. The values represent the mean + the standard error for quadruplicate determinations.

As Fig.6B shows, serum stimulation of Gα₁₂QL transfected indicator cells dramatically increased arachidonic acid release relative to cells stably transfected with pcDNA3, ras, or Gα₁₃QL expression vectors as shown in Fig. 6A. These results demonstrate the apparent specificity with which Gα₁₂QL expression stimulates the COX-2 pathway. Activation or inhibition of COX-2 activity in Gα₁₂QL transfected indicator cells can be assessed by determining the levels of arachidonic acid produced by such indicator cells. The assay may be used to screen candidate compounds for inhibition of COX-2 activity. All references cited with respect to synthetic, preparative and analytical procedures are incorporated herein by reference. All sequence records identified by GenBank accession numbers are incorporated herein by reference. The present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof and, accordingly, reference should be made to the appended claims, ratherthan to the foregoing specification, as indication the scope of the invention.

Claims

What is claimed is:

1. A method for screening a test substance for COX-2 inhibitory activity comprising:

(a) contacting the test substance with indicator cells which constitutively express endogenous COX-2 or inducibly express endogenous COX-2; and

(b) determining the level of:

(i) proliferation of the indicator cells in the presence and absence of the test substance, a decreased level of proliferation of the indicator cells in the presence of the test substance indicating that the test substance has COX-2 inhibitory activity; or

(ii) one or more prostaglandins produced by the indicator cells in the presence and absence of the test substance, a decreased prostaglandin level in the presence of the test substance indicating that the test substance has COX-2 inhibitory activity.

(iii) arachidonic acid produced by the indicator cells in the presence and absence of the test substance, an increased arachidonic acid level in the presence of the test substance indicating that the test substance has COX-2 inhibitory activity.

2. A method for screening a test substance for COX-2 inhibitory activity comprising:

(a) contacting the test substance with indicator cells which express a GTPase-deficient mutant form of the α-subunit of protein G12, which mutant α-subunit has the capacity to induce the production of arachidonic acid and COX-2 in the indicator cells; and

(b) determining the level of proliferation of the indicator cells in the presence and absence of the test substance, a decreased level of proliferation of the indicator cells in the presence of the test substance indicating that the test substance has COX-2 inhibitory activity.

3. A method according to claim 2 wherein the G12 protein α- subunit mutant comprises the Q229L mutation.

4. A method according to claim 3 wherein the level of indicator cell proliferation is determined by an assay for DNA synthesis by the indicator cells.

5. A method according to claim 4 wherein the DNA synthesis assay comprises assaying tritium-labeled thymidine uptake by the indicator cells.

6. A method for screening a test substance for COX-2 inhibitory activity comprising:

(b) determining the level of one or more prostaglandins produced by the indicator cells in the presence and absence of the test substance, a decrease in production of said prostaglandin by the indicator cells in the presence of the test substance indicating that the test substance has COX- 2 inhibitory activity.

7. A method according to claim 6 wherein the G12 protein α- subunit mutant comprises the Q229L mutation.

8. A method according to claim 7 wherein the prostaglandin level is assayed in the media surrounding the indicator cells, in the indicator cells or in a component of the indicator cells.

9. A method according to claim 8 wherein the prostaglandin level is assayed by a prostaglandin immunoassay.

10. A method according to claim 9 wherein the prostaglandin immunoassay comprises a competitive immunoassay.

12. A method for screening a test substance for COX-2 inhibitory activity comprising:

(b) determining the level of arachidonic acid provided by the indicator cells in the presence and absence of the test substance, an increase in the level of arachidonic acid provided by the indicator cells in the presence of the test substance indicating that the test substance has COX-2 inhibitory activity.

13. A method according to claim 12 wherein the G12 protein α- subunit mutant comprises the Q229L mutation.

14. A method according to claim 13 wherein the arachidonic acid level is assayed in the media surrounding the indicator cells, in the indicator cells or in a component of the indicator cells.

15. A method according to claim 14 wherein the arachidonic acid assay comprises assaying tritium labeled arachidonic acid release from said indicator cells.

CELL-BASED ASSAY FOR SCREENING COX-2 INHIBITORS