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EP1027121A4 - Improved liquid chromatographic media for polynucleotide separation - Google Patents

Improved liquid chromatographic media for polynucleotide separation

Info

Publication number
EP1027121A4
EP1027121A4 EP98918701A EP98918701A EP1027121A4 EP 1027121 A4 EP1027121 A4 EP 1027121A4 EP 98918701 A EP98918701 A EP 98918701A EP 98918701 A EP98918701 A EP 98918701A EP 1027121 A4 EP1027121 A4 EP 1027121A4
Authority
EP
European Patent Office
Prior art keywords
liquid chromatographic
improved liquid
chromatographic media
polynucleotide separation
polynucleotide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP98918701A
Other languages
German (de)
French (fr)
Other versions
EP1027121A1 (en
Inventor
Douglas T Gjerde
Paul D Taylor
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Precipio Inc
Original Assignee
Transgenomic Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Transgenomic Inc filed Critical Transgenomic Inc
Publication of EP1027121A1 publication Critical patent/EP1027121A1/en
Publication of EP1027121A4 publication Critical patent/EP1027121A4/en
Withdrawn legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3231—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the coating or impregnating layer
    • B01J20/3242—Layers with a functional group, e.g. an affinity material, a ligand, a reactant or a complexing group
    • B01J20/3268—Macromolecular compounds
    • B01J20/328—Polymers on the carrier being further modified
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
    • B01J20/286—Phases chemically bonded to a substrate, e.g. to silica or to polymers
    • B01J20/287—Non-polar phases; Reversed phases
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3214—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the method for obtaining this coating or impregnating
    • B01J20/3225—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the method for obtaining this coating or impregnating involving a post-treatment of the coated or impregnated product
    • B01J20/3227—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the method for obtaining this coating or impregnating involving a post-treatment of the coated or impregnated product by end-capping, i.e. with or after the introduction of functional or ligand groups
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3231—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the coating or impregnating layer
    • B01J20/3242—Layers with a functional group, e.g. an affinity material, a ligand, a reactant or a complexing group
    • B01J20/3244—Non-macromolecular compounds

Landscapes

  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
EP98918701A 1997-04-25 1998-04-24 Improved liquid chromatographic media for polynucleotide separation Withdrawn EP1027121A4 (en)

Applications Claiming Priority (19)

Application Number Priority Date Filing Date Title
US4485697P 1997-04-25 1997-04-25
US44856P 1997-04-25
US5545697P 1997-08-11 1997-08-11
US55456P 1997-08-11
US5952797P 1997-09-22 1997-09-22
US59527P 1997-09-22
US6212397P 1997-10-15 1997-10-15
US62123P 1997-10-15
US6230397P 1997-10-17 1997-10-17
US62303P 1997-10-17
US6361997P 1997-10-27 1997-10-27
US63619P 1997-10-27
US6931397P 1997-12-05 1997-12-05
US69313P 1997-12-05
US7799898P 1998-03-13 1998-03-13
US77998P 1998-03-13
US5833798A 1998-04-10 1998-04-10
US58337 1998-04-10
PCT/US1998/008293 WO1998048914A1 (en) 1997-04-25 1998-04-24 Improved liquid chromatographic media for polynucleotide separation

Publications (2)

Publication Number Publication Date
EP1027121A1 EP1027121A1 (en) 2000-08-16
EP1027121A4 true EP1027121A4 (en) 2001-07-11

Family

ID=27578183

Family Applications (1)

Application Number Title Priority Date Filing Date
EP98918701A Withdrawn EP1027121A4 (en) 1997-04-25 1998-04-24 Improved liquid chromatographic media for polynucleotide separation

Country Status (5)

Country Link
EP (1) EP1027121A4 (en)
JP (1) JP2002506425A (en)
AU (1) AU725928B2 (en)
CA (1) CA2285307A1 (en)
WO (1) WO1998048914A1 (en)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6475388B1 (en) 1996-11-13 2002-11-05 Transgenomic, Inc. Method and system for RNA analysis by matched ion polynucleotide chromatography
US6576133B2 (en) 1996-11-13 2003-06-10 Transgenomic, Inc Method and system for RNA analysis by matched ion polynucleotide chromatography
US6177559B1 (en) 1998-04-24 2001-01-23 Transgenomic, Inc. Process for separation of polynucleotide fragments
EP1056528A4 (en) * 1997-10-30 2002-02-20 Transgenomic Inc Method for high resolution liquid chromatographic separation of polynucleotides
US6258264B1 (en) 1998-04-10 2001-07-10 Transgenomic, Inc. Non-polar media for polynucleotide separations
US6265168B1 (en) 1998-10-06 2001-07-24 Transgenomic, Inc. Apparatus and method for separating and purifying polynucleotides
JP2003532088A (en) * 2000-05-01 2003-10-28 トランスジエノミツク・インコーポレーテツド Polynucleotide separation on polymer separation media
CA2396667A1 (en) * 2000-05-26 2001-12-06 Benjamin L. Legendre, Jr. Apparatus and method for separating and purifying polynucleotides
US6521411B2 (en) 2000-09-28 2003-02-18 Transgenomic, Inc. Method and system for the preparation of cDNA
JP4069999B2 (en) * 2001-03-13 2008-04-02 独立行政法人理化学研究所 Nano-coated molecular material
DE10120821A1 (en) * 2001-04-27 2002-10-31 Sachtleben Chemie Gmbh A polyalkene coated porous filler useful as a packing material in high performance chromatography is stable towards solvents and strong acids or bases, and avoids excessive preparation costs due to the high solvent volumes required
WO2003031580A2 (en) 2001-10-05 2003-04-17 Transgenomic, Inc. Methods and compositions for mutation analysis by liquid chromatography
WO2012033194A1 (en) * 2010-09-09 2012-03-15 ダイセル化学工業株式会社 Method for separating water-soluble biological material
EP2570183A1 (en) * 2011-09-15 2013-03-20 InstrAction GmbH Sorbent comprising on its surface an aliphatic unit for the purification of organic molecules
TW201726917A (en) * 2015-12-28 2017-08-01 昭和電工股份有限公司 Separation and analysis method
MX2018013486A (en) 2016-05-06 2019-03-28 Goldcorp Inc Adsorbent composition, method of making the same, and uses thereof.

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4042327A (en) * 1976-01-15 1977-08-16 Waters Associates, Inc. Ion-pairing chromatography
US5015373A (en) * 1988-02-03 1991-05-14 Regents Of The University Of Minnesota High stability porous zirconium oxide spherules
US5100547A (en) * 1991-03-04 1992-03-31 Monsanto Company Chromatography supports consisting of an inorganic susbstrate coated with polybutadiene with alkyl side chains
WO1992015385A1 (en) * 1991-03-04 1992-09-17 Simon Ethan S Butadiene acrylonitrile polymeric coating and chromatographic packing material
US5585236A (en) * 1992-11-18 1996-12-17 Sarasep, Inc. Nucleic acid separation on alkylated nonporous polymer beads
US5772889A (en) * 1995-11-13 1998-06-30 Transgenomic, Inc. System and method for performing nucleic acid separations using liquid chromatography

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0637510B2 (en) * 1985-10-04 1994-05-18 東ソー株式会社 Nucleic acid separation method

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4042327A (en) * 1976-01-15 1977-08-16 Waters Associates, Inc. Ion-pairing chromatography
US5015373A (en) * 1988-02-03 1991-05-14 Regents Of The University Of Minnesota High stability porous zirconium oxide spherules
US5100547A (en) * 1991-03-04 1992-03-31 Monsanto Company Chromatography supports consisting of an inorganic susbstrate coated with polybutadiene with alkyl side chains
WO1992015385A1 (en) * 1991-03-04 1992-09-17 Simon Ethan S Butadiene acrylonitrile polymeric coating and chromatographic packing material
US5585236A (en) * 1992-11-18 1996-12-17 Sarasep, Inc. Nucleic acid separation on alkylated nonporous polymer beads
US5772889A (en) * 1995-11-13 1998-06-30 Transgenomic, Inc. System and method for performing nucleic acid separations using liquid chromatography

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
ERIKSSON S ET AL: "SEPARATION OF DNA RESTRICTION FRAGMENTS BY ION-PAIR CHROMATOGRAPHY", JOURNAL OF CHROMATOGRAPHY,NL,ELSEVIER SCIENCE PUBLISHERS B.V. AMSTERDAM, vol. 359, January 1986 (1986-01-01), pages 265 - 274, XP002910334, ISSN: 0021-9673 *
Huber et al. High-Resolution Liquid Chromatography of Oligonucleotides on Nonporous Alkylated Styrene-Divinylbenzene Copolymers. (Cited in the international search report). *
MAA et al. Rapid High-Perfomance Liquid Chromatography of Nucleic Acids With Polystyrene-Based Micropellicular Anion Exchangers (Cited in the international search report) *
See also references of WO9848914A1 *

Also Published As

Publication number Publication date
CA2285307A1 (en) 1998-11-05
EP1027121A1 (en) 2000-08-16
AU7157798A (en) 1998-11-24
AU725928B2 (en) 2000-10-26
JP2002506425A (en) 2002-02-26
WO1998048914A1 (en) 1998-11-05

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