[go: up one dir, main page]

EP0217341A2 - Substance anticoagulante, procédé pour la préparer et anticoagulant la comportant en tant que principe actif - Google Patents

Substance anticoagulante, procédé pour la préparer et anticoagulant la comportant en tant que principe actif Download PDF

Info

Publication number
EP0217341A2
EP0217341A2 EP86113375A EP86113375A EP0217341A2 EP 0217341 A2 EP0217341 A2 EP 0217341A2 EP 86113375 A EP86113375 A EP 86113375A EP 86113375 A EP86113375 A EP 86113375A EP 0217341 A2 EP0217341 A2 EP 0217341A2
Authority
EP
European Patent Office
Prior art keywords
substance
prolonging
minutes
anticoagulating
activity
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP86113375A
Other languages
German (de)
English (en)
Other versions
EP0217341B2 (fr
EP0217341A3 (en
EP0217341B1 (fr
Inventor
Masahiro Maki
Hideo Tani
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Kowa Co Ltd
Original Assignee
Kowa Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=16705389&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=EP0217341(A2) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Kowa Co Ltd filed Critical Kowa Co Ltd
Publication of EP0217341A2 publication Critical patent/EP0217341A2/fr
Publication of EP0217341A3 publication Critical patent/EP0217341A3/en
Application granted granted Critical
Publication of EP0217341B1 publication Critical patent/EP0217341B1/fr
Publication of EP0217341B2 publication Critical patent/EP0217341B2/fr
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Images

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/48—Reproductive organs
    • A61K35/50—Placenta; Placental stem cells; Amniotic fluid; Amnion; Amniotic stem cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4721—Lipocortins
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/02—Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • G—PHYSICS
    • G03—PHOTOGRAPHY; CINEMATOGRAPHY; ANALOGOUS TECHNIQUES USING WAVES OTHER THAN OPTICAL WAVES; ELECTROGRAPHY; HOLOGRAPHY
    • G03C—PHOTOSENSITIVE MATERIALS FOR PHOTOGRAPHIC PURPOSES; PHOTOGRAPHIC PROCESSES, e.g. CINE, X-RAY, COLOUR, STEREO-PHOTOGRAPHIC PROCESSES; AUXILIARY PROCESSES IN PHOTOGRAPHY
    • G03C1/00—Photosensitive materials
    • G03C1/005—Silver halide emulsions; Preparation thereof; Physical treatment thereof; Incorporation of additives therein
    • G03C1/04—Silver halide emulsions; Preparation thereof; Physical treatment thereof; Incorporation of additives therein with macromolecular additives; with layer-forming substances
    • G03C1/047—Proteins, e.g. gelatine derivatives; Hydrolysis or extraction products of proteins
    • G03C2001/0471—Isoelectric point of gelatine
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/827—Proteins from mammals or birds
    • Y10S530/842—Skin; hair; nails; sebaceous glands; cerumen
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/827—Proteins from mammals or birds
    • Y10S530/85—Reproductive organs or embryos
    • Y10S530/851—Placenta; amniotic fluid

Definitions

  • This invention relates to a novel anticoagulating substance, a process for preparing the substance, an anticoagulant comprising the substance as an effective component, and a medical composition.
  • the coagulation of blood is considered to start from the development of thromboplastin activity, followed by activation of factor X and V in the blood, further activation of prothrombin into thrombin and final conversion of fibrinogen into fibrin by the action of the thrombin.
  • anticoagulating substances include heparin, heparin cofactor-II, antithrombin-III, 9 2 -macroglobulin, ⁇ 1 - trypsin inhibitor, C l -esterase inhibitor, protein C and the like.
  • the present inventors made intensive studies on anticoagulants which are free of side effects and harmless to the living body. As a result, it has been found that a novel anticoagulating substance is obtained from placentae, particularly human placentae, which contain a large amount of tissue thromboplastin along with the factor XIII and the fibrinolytic inhibition factor, which is considered to have a tendency toward thrombotic formation and which has a specific state from the standpoint of the coagulating mechanism.
  • the novel anticoagulation substance which is described above is isolated from a homogenate of human placentae, especially from residue and microsome fraction in the supernatant obtained by centrifugal separation of the homogenate of human placentae. The present invention is accomplished based on the above finding.
  • the present invention provides a human placenta-derived, novel anticoagulating substance having the following properties, a process for preparing the same and an anticoagulant having the substance as its effective component.
  • the anticoagulating substance of the invention (which may be hereinafter referred to as "present substance”) can be prepared, for example, as follows.
  • a placenta homogenate is first prepared from the human placenta and centrifugally separated.
  • the homogenating operation is effected as follows: the amnion is removed from the placenta and sufficiently washed with a physiological saline solution, followed by homogenization by the use of the Waring blender and Polytron. The resulting homogenate is subjected to centrifugal separation to obtain a supernatant and a sediment or residue.
  • the supernatant is further fractionated by ultracentrifugal separation at 50,000 to 100,000 x g to get a microsome fraction.
  • the residue separated from homogenate and the thus obtained microsome fraction are washed enough with a buffer solution, and are collected respectively by centrifugal or ultracentrifugal separation as washed residue and microsome. Both washed materials are combined together and immersed in a solution of chelating agent and/or surface active agent.
  • the chelating agents include such as EDTA, EGTA, citrate, oxalate, nitrilotriacetic acid chelate, phosphoric acid and the like
  • the surface active agents include such as Triton@ X - 100, Lubrol®, SDS, deoxycholic acid and the like.
  • the ammonium sulfate fractionation is effected by two step procedure. First, solid ammonium sulfate is added to the extract until 35% saturation concentration, and a supernatant is subjected to centrifugal separation. Then, solid ammonium sulfate is added to the supernatant until reaching 85% saturation concentration and obtained ammonium sulfate fraction as a precipitate by centrifugal separation.
  • the resulting ammonium sulfate fraction is purified by known isolation and purification procedures including, for example, dialysis, ion exchange chromatography, gel filtration, adsorption chromatography, hydrophobic chromatography, isoelectric point column electrophoresis, affinity chromatography using lectin or an antibody and the like, thereby obtaining the present substance. These procedures may be used singly or in combination. For example, a fraction obtained by subjecting the extract with a chelating agent and/or a surface active agent to ammonium sulfate fractionation is sufficiently dialyzed.
  • the resulting dialyzate is subjected to elution according to a linear concentration gradient method using DEAE-Toyopearl and the resultant active fraction is dialyzed, followed by passage through Blue Sepharose. Subsequently, the active fraction is concentrated and subjected to gel filtration using Sephadex G-100 to obtain the present substance.
  • the thus obtained present substance has such properties as indicated before and the measurements and results for these properties are more particularly described.
  • the molecular weight measured by SDS-polyacrylamide gel electrophoresis (12% polyacrylamide gel; reduced state) was found to be 34,000 + 2,000.
  • the present substance was thermally treated at different temperatures (0 - 90°C) for 30 minutes and the anticoagulating activity was measured according to the recalcification time method. As a result, it was found that the activity completely disappeared at temperatures not lower than 50°C.
  • Buffer solutions having different pH values of 2.5 to 10.0 were added, followed by treatment at 4°C for 18 hours and at 25°C for 3 hours. Thereafter, the remaining anticoagulating activity was measured by the recalcification method. As a result, the activity did not lower in the pH range of 4 to 10.
  • the present substance was added to plasma and incubated at 37°C for 30 minutes, followed by measurement of a remaining anticoagulating activity by the recalcification time method. No lowering of the activity was found.
  • PT reagents Simplastin: Warner Lambert Co., Ltd.
  • 100 microliters of a solution of the present substance were mixed. After 3 minutes, 100 microliters of standard plasma were added, followed by measurement of the coagulation time. The results are shown in Table 2, revealing a strong PT-prolonging action.
  • thromboplastin time APTT
  • APTT reagent activate thrombophax, Ortho Diagnostic Systems Inc.
  • 90 microliters of a solution of the present substance were mixed. After 2 minutes, 100 microliters of standard plasma was added to the mixture. After 6 minutes, 100 microliters of a 0.025 M calcium chloride solution was added in order to determine the coagulation time. As a result, a strong APTT prolonging action was recognized as shown in Table 3.
  • the present substance was hydrolyzed with 6N hydrochloric acid at 110°C for 24 hours and subjected to an amino acid analyzing system of the Waters high performance liquid chromatography (Waters HPLC). The results are shown in Table 4.
  • the preparation may be an injection.
  • the injection is preferably in the form where a freeze-dried powder is dissolved in distilled water or physiological saline solution for injection and administered.
  • the injection site may conveniently be intravenous.
  • the amount of administration is preferably in the range of from 10 g to 10 mg/kg.day. It should be noted that the present substance presents no abnormality on use within the above range of the amount of administration and is thus harmless.
  • albumin, gelatin or mannitol may be added as a stabilizer. The addition of these stabilizers can prevent decomposition and adsorption during the preparation process and can improve the storage stability of the preparation.
  • the present substance has a high anticoagulating activity and exhibits a higher activity in the state where the tissue thromboplastin activity is exacerbated or in the state of exacerbating the coagulation.
  • the anticoagulant containing the substance as an effective component has reduced side effects and is thus harmless.
  • the dialyzate was adsorbed on DEAE-Toyopearl ( ⁇ 5.5 x 19 cm) equilibrated with a 20 mM tris-hydrochloric acid buffer solution (pH 7.4) and sufficiently washed with the buffer solution. Thereafter, 4 liters of the same buffer solutions containing from 0 to 0.3 M sodium chloride were used to cause 20 ml of each fraction to be eluted according to the linear concentration gradient method. The active fraction was found to be eluted in the vicinity of a sodium chloride concentration of approximately 0.15 M and 380 ml of the active fraction was obtained.
  • Example 2 One gram of the washed sediment obtained in (i) of Example 1 was mixed with 1 ml of each of 50 mM tris-hydrochloric acid buffer solutions (pH 7.4) containing different concentrations of EDTA (0 - 200 mM) and homogenized, followed by allowing to stand at 4°C overnight and centrifugal separation at 7,000 r.p.m. for 15 minutes to obtain 1 ml of a supernatant. The supernatant was subsequently subjected to SDS-polyacrylamide gel electrophoresis and western plotting and then to immunological staining with rabbit antiserum, followed by quantitative determination of the present substance by means of a densitometer. The results are shown in Table 5 below.
  • the present substance can be efficiently extracted using an equivalent tris-hydrochloric buffer solution containing not less than 12.5 mM, preferably from 20 to 100 mM, of EDTA.
  • the thus obtained supernatant was subjected to SDS-polyacrylamide gel electrophoresis, followed by dyeing proteins to detect a protein band at the same mobility position as detected for the inventive substance.
  • the samples which revealed the protein band were further subjected to the steps (iii), (iv), (v) and (vi) of Example 1, i.e, fractionation by ammonium sulfate, ion exchange by DEAE-Toyopearl (o 1.4 x 7 cm), passage through on Blue Sepharose ( ⁇ 1.4 x 6.5 cm) and gel filtration by Sephadex G-100 ( ⁇ 2.0 x 90 cm) in this order to obtain a purified inventive substance.
  • the present substance can be efficiently extracted or purified by using a chelating agent having a strong chelating effect to calcium and a tris-hydrochloric acid buffer solution of at least equimolar to a washed sediment.
  • the present substance is purified from Lubrol extraction by the following way. 40 ml of a 50 mM tris-hydrochloric acid buffer solution (pH 7.4) containing 1% of Lubrol was added to 10 g of a washed sediment and homogenized. The resulting homogenate was allowed to stand at 4°C overnight and centrifugally separated at 7,000 r.p.m. for 15 minutes to collect a supernatant. 40 ml of the supernatant was subjected to a gel filtration using Sephadex G-100 ( ⁇ 4.8 x 75 cm), followed by ion exchange by DEAE-Toyopearl ( ⁇ 1.4 x 7 cm). Thereafter, the above sample was subjected to a gel filtration using Sephadex G-100 ( ⁇ 2.0 x 90 cm) again to obtain 0.95 mg (determined by Lowry Method) of the purified inventive substance.
  • the extract prepared in the same manner as in (i) and (ii) of Example 1 was mixed with ammonium sulfate solutions having different saturations of 40 to 80% and allowed to stand at 4°C for 30 minutes or over, followed by centrifugal separation at 7,000 r.p.m. for 15 minutes to separate a supernatant and a precipitate from each other.
  • the present substance can be isolated by treating the chelating agent extract with an ammonium sulfate solution having a saturation not higher than 40% to remove impurities therefrom and concentrating the treated extract by the use of an ammonium sulfate solution having a saturation of not less than 80%.
  • the sediment was suspended in 500 ml of cold ether and shaked, followed by centrifugal separation at 2,000 r.p.m. for 10 minutes to collect a sediment.
  • the sediment was suspended in about 50 ml of a 20 mM tris-hydrochloric acid buffer solution (pH 7.4) and, after supersonic treatment, subjected to ultracentrifugation at 105,000 g for 1 hour to obtain 53 ml of a supernatant (microsome-soluble fraction).
  • Example 1 The present substance obtained in Example 1 was subjected to measurement of an anticoagulating action by a thromboelastograph.
  • the bloods used were those of rabbit and men. The results are indicated by ratios to a control value which is obtained without addition of the present substance and shown in Table 9.
  • the anticoagulating activity of the present substance is better when added in such a state that the coagulating system starts to function than immediately after the collection of the blood.
  • TTP was added to the rabbit blood immediately after its collectin in amounts of 0 to 4.0 ⁇ g/ml (final concentration), followed by measurement of the coagulation time in the same manner as in experiment (1). The results are shown in Fig. 1.
  • Fig. 1 reveal that when TTP was added about 1 ⁇ g/ml, the coagulation time can be shortened to a half, thus causing the coagulation to be promoted.
  • the above ingredients were dissolved in 2 ml distilled water for injection and placed in a sterile vial, followed by preliminary freezing at -30°C to -40°C for 2 hours, primary drying at a temperature of -30°C to +20°C under a vacuum of 0.05 to 0.1 Torr. for 35 hours and then secondary drying at 30°C under a vacuum of 0.01 to 0.05 Torrs. for 5 hour, to obtain a vial for injection.

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Biophysics (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Animal Behavior & Ethology (AREA)
  • Genetics & Genomics (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Toxicology (AREA)
  • Cell Biology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biochemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Reproductive Health (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Hematology (AREA)
  • Diabetes (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Pregnancy & Childbirth (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Immunology (AREA)
  • Virology (AREA)
  • Epidemiology (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
EP86113375A 1985-09-30 1986-09-29 Procédé pour la préparation d'une substance anticoagulante Expired - Lifetime EP0217341B2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP217512/85 1985-09-30
JP21751285 1985-09-30

Publications (4)

Publication Number Publication Date
EP0217341A2 true EP0217341A2 (fr) 1987-04-08
EP0217341A3 EP0217341A3 (en) 1988-04-06
EP0217341B1 EP0217341B1 (fr) 1990-12-12
EP0217341B2 EP0217341B2 (fr) 1997-12-17

Family

ID=16705389

Family Applications (1)

Application Number Title Priority Date Filing Date
EP86113375A Expired - Lifetime EP0217341B2 (fr) 1985-09-30 1986-09-29 Procédé pour la préparation d'une substance anticoagulante

Country Status (7)

Country Link
US (1) US4732891A (fr)
EP (1) EP0217341B2 (fr)
JP (1) JPH0710880B2 (fr)
KR (1) KR940003751B1 (fr)
CA (1) CA1265446A (fr)
DE (1) DE3676148D1 (fr)
ES (1) ES2001707A6 (fr)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0264060A2 (fr) * 1986-10-14 1988-04-20 Kowa Company, Ltd. Anticoagulant et son procédé de préparation
EP0267601A2 (fr) * 1986-11-14 1988-05-18 Kowa Co. Ltd. Anticorps monoclonal anti-PCI
EP0271885A2 (fr) * 1986-12-18 1988-06-22 BEHRINGWERKE Aktiengesellschaft Médicament contenant la protéine tissulaire PP4, procédé de préparation de PP4 et sa pasteurisation et l'utilisation de PP4
EP0279459A2 (fr) * 1987-02-20 1988-08-24 Kowa Company, Ltd. DNA codant pour un polypeptide anticoagulant
EP0318703A1 (fr) * 1987-11-03 1989-06-07 BEHRINGWERKE Aktiengesellschaft Préparation par génie génétique de la protéine anticoagulante PP4

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5066788A (en) * 1984-09-21 1991-11-19 Boehringer Ingelheim International Gmbh Blood coagulation inhibiting proteins, processes for preparing them and their uses
US5264550A (en) * 1985-04-15 1993-11-23 Scios Nova Inc. Human anti-inflammatory phospholipase inhibitor protein
US5047503A (en) * 1986-07-15 1991-09-10 Kowa Company, Ltd. Thrombin-binding substance and process for its preparation
US4937324A (en) * 1987-02-06 1990-06-26 Zymogenetics, Inc. Chromatographic purification of human proteins having anticoagulant and anti-inflammatory activity
PT87083B (pt) * 1987-03-28 1992-07-31 Boehringer Ingelheim Int Processo para a preparacao de uma proteina anticoagulante vascular, de adn que codifica para esta proteina e de composicoes farmaceuticas que a contem
DE3724726A1 (de) * 1987-07-25 1989-02-02 Behringwerke Ag Verfahren zur reinigung des plazentaren gewebeproteins pp4
DE3737237A1 (de) * 1987-11-03 1989-05-18 Behringwerke Ag Anticoagulatorisches protein pp4-x, seine herstellung und verwendung
JP2660548B2 (ja) * 1988-06-15 1997-10-08 興和株式会社 膠原病疾患関連抗体検出用試薬及びこれを用いる膠原病疾患関連抗体の検出方法
US5202421A (en) * 1988-12-27 1993-04-13 Mochida Pharmaceutical Co., Ltd. Anticoagulant substance obtained from urine and process for the preparation thereof
ES2055234T3 (es) * 1989-07-15 1994-08-16 Boehringer Ingelheim Int Agente con contenido de anticoagulante.
US5360789A (en) * 1990-11-20 1994-11-01 Kowa Co., Ltd. Therapeutic agent for skin or corneal disease
US20030220233A1 (en) 1994-01-24 2003-11-27 Neorx Corporation Radiolabeled annexins
US5968477A (en) * 1994-01-24 1999-10-19 Neorx Corporation Radiolabeled annexin conjugates with hexose and a chelator
AU5062100A (en) 1999-06-14 2001-01-02 Novo Nordisk A/S Fviia/tf activity inhibiting compounds
CA2385371A1 (fr) * 2000-07-21 2002-01-31 Hiroshi Mizokami Procede de purification de la proteine de liaison a l'ion calcium
US20090291086A1 (en) * 2001-02-21 2009-11-26 Alavita Pharmaceuticals, Inc. Compositions and Methods for Treating Cerebral Thrombosis and Global Cerebral Ischemia
US7645739B2 (en) * 2001-02-21 2010-01-12 Alavita Pharmaceuticals, Inc. Modified annexin compositions and methods of using same
US7635676B2 (en) * 2001-02-21 2009-12-22 Alavita Pharmaccuticals, Inc. Modified annexin proteins and methods for their use in organ transplantation
WO2002067857A2 (fr) * 2001-02-21 2002-09-06 Surromed, Inc. Annexines modifiées et prévention de la thrombose
US7635680B2 (en) * 2001-02-21 2009-12-22 Alavita Pharmaceuticals, Inc. Attenuation of reperfusion injury
CA2439100A1 (fr) * 2001-02-26 2002-09-06 Kowa Company, Ltd. Gouttes ophtalmiques
HUP0401124A3 (en) 2001-03-22 2006-01-30 Novo Nordisk Healthcare Ag Coagulation factor vii derivatives
US6982154B2 (en) * 2002-02-21 2006-01-03 Surromed, Inc. Modified annexin proteins and methods for treating vaso-occlusive sickle-cell disease

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3501567A (en) * 1962-08-31 1970-03-17 Lab Choay Euglobulinic protein fraction for sensitization of fibrinolytic proenzymes in human blood and preparation thereof from human placental tissue
AT318136B (de) * 1971-08-24 1974-09-25 Behringwerke Ag Verfahren zur Isolierung thromboplastischen Materials
DE2720704A1 (de) * 1977-05-07 1978-11-16 Behringwerke Ag Neues glycoprotein und verfahren zu dessen herstellung
JPS60152421A (ja) * 1984-01-23 1985-08-10 Sanwa Kagaku Kenkyusho:Kk ヒト胎盤性プラスミノ−ゲン活性化因子、及びそれとヒトプラスミノ−ゲン活性化阻害因子との結合物、及びそれらの製法
EP0182929A1 (fr) * 1984-11-26 1986-06-04 American Hospital Supply Corporation Coactivateur pour l'activation de la protéine C

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3310471A (en) * 1965-05-13 1967-03-21 Upjohn Co Placental lactogenic factor process
US3409605A (en) * 1965-06-15 1968-11-05 American Cyanamid Co Concentration and purification of growth factor-placental origin (human)
DE2640387C3 (de) * 1976-09-08 1981-01-22 Behringwerke Ag, 3550 Marburg Gewebespezifisches Protein und Verfahren zu dessen Herstellung
IT1114943B (it) * 1977-12-27 1986-02-03 Sclavo Inst Sieroterapeut Processo per la preparazione di somatomammotropina corionica umana
DE2842467A1 (de) * 1978-09-29 1980-04-10 Behringwerke Ag Neues ubiquitaeres gewebsprotein pp tief 8
DE2952792A1 (de) * 1979-12-31 1981-07-02 Behringwerke Ag, 3550 Marburg Neues protein (pp(pfeil abwaerts)15(pfeil abwaerts)) mit immunsuppressiver wirkung
DE3230996A1 (de) * 1982-08-20 1984-02-23 Behringwerke Ag, 3550 Marburg Neues protein (pp(pfeil abwaerts)1(pfeil abwaerts)(pfeil abwaerts)3(pfeil abwaerts)), verfahren zu seiner anreicherung und gewinnung sowie seine verwendung
DE3315000A1 (de) * 1983-04-26 1984-10-31 Behringwerke Ag, 3550 Marburg Gewebeprotein pp(pfeil abwaerts)4(pfeil abwaerts), verfahren zu seiner gewinnung sowie seine verwendung
JPS6019719A (ja) * 1983-07-15 1985-01-31 Asahi Chem Ind Co Ltd 抗腫瘍活性を有する蛋白質
DE3338480A1 (de) * 1983-10-22 1985-05-02 Behringwerke Ag, 3550 Marburg Neues protein pp(pfeil abwaerts)2(pfeil abwaerts)(pfeil abwaerts)0(pfeil abwaerts), verfahren zu seiner gewinnung sowie seine verwendung
DE3404563A1 (de) * 1984-02-09 1985-08-14 Behringwerke Ag, 3550 Marburg Gewebeprotein pp(pfeil abwaerts)1(pfeil abwaerts)(pfeil abwaerts)9(pfeil abwaerts), verfahren zu seiner gewinnung sowie seine verwendung

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3501567A (en) * 1962-08-31 1970-03-17 Lab Choay Euglobulinic protein fraction for sensitization of fibrinolytic proenzymes in human blood and preparation thereof from human placental tissue
AT318136B (de) * 1971-08-24 1974-09-25 Behringwerke Ag Verfahren zur Isolierung thromboplastischen Materials
DE2720704A1 (de) * 1977-05-07 1978-11-16 Behringwerke Ag Neues glycoprotein und verfahren zu dessen herstellung
JPS60152421A (ja) * 1984-01-23 1985-08-10 Sanwa Kagaku Kenkyusho:Kk ヒト胎盤性プラスミノ−ゲン活性化因子、及びそれとヒトプラスミノ−ゲン活性化阻害因子との結合物、及びそれらの製法
EP0182929A1 (fr) * 1984-11-26 1986-06-04 American Hospital Supply Corporation Coactivateur pour l'activation de la protéine C

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
CHEMICAL ABSTRACTS, vol. 104, no. 2, 13th January 1986, page 317, abstract no. 10572t, Columbus, Ohio, US; & JP - A - 60 152 421 (JAPAN KOKAI TOKKYO KOHO)10-08-1985 (Cat. X) *
CHEMICAL ABSTRACTS, vol. 68, no. 21, 20th May 1968, page 9051, abstract no. 94005j, Columbus, Ohio, US; R. SLUNSKY "Activators and inhibitors of fibrinolysis in amniotic fluid and in placenta, decidua, and myometrium extracts of interrupted and completed pregnancies", & FOLIA HAEMATOL. 1967, 88(2-3), 236-243 *
CHEMICAL ABSTRACTS, vol. 70, no. 5, 3rd February 1969, page 17255, abstract no. 17266j, Columbus, Ohio, US; O. PONARI et al.: "New fibrinolytic agent extracted from human placenta ("thromboclase"). I. In vitro study of total activity and of different fibrinolytic factors", & THERAPIE 1968, 23(4), 737-750 *
CHEMICAL ABSTRACTS, vol. 86, no. 19, 9th May 1977, page 336, abstract no. 136895d, Columbus, Ohio, US; P. BIANCHINI et al.: "Biologically active substances in placental extracts", & FARMACO, ED. PRAT. 1977, 32(3), 139-151 *
CHEMICAL ABSTRACTS, vol. 92, no. 11, 17th March 1980, page 355, abstract no. 91573c, Columbus, Ohio, US; M. USZYNSKI et al.: "Anticoagulant activity of peptides from the human placenta", & THROMB. RES. 1979, 16(5-6),689-694 *

Cited By (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0264060A2 (fr) * 1986-10-14 1988-04-20 Kowa Company, Ltd. Anticoagulant et son procédé de préparation
EP0264060A3 (en) * 1986-10-14 1988-07-27 Kowa Company, Ltd. Anticoagulating substance and preparation process thereof
US4873222A (en) * 1986-10-14 1989-10-10 Kowa Co, Ltd. Placenta-derived anticoagulating substance
EP0267601A2 (fr) * 1986-11-14 1988-05-18 Kowa Co. Ltd. Anticorps monoclonal anti-PCI
EP0267601A3 (en) * 1986-11-14 1988-10-12 Kowa Co. Ltd. Anti-pci monoclonal antibody
EP0271885A2 (fr) * 1986-12-18 1988-06-22 BEHRINGWERKE Aktiengesellschaft Médicament contenant la protéine tissulaire PP4, procédé de préparation de PP4 et sa pasteurisation et l'utilisation de PP4
EP0271885A3 (en) * 1986-12-18 1988-09-21 Behringwerke Aktiengesellschaft Medicine containing the tissular protein pp4, process for the preparation of pp4 and its pasteurization, and the use of pp4
US5097019A (en) * 1986-12-18 1992-03-17 Behringwerke Aktiengesellschaft Pharmaceutical containing tissue protein pp4, a process for the preparation of pp4 and for the pasteurization thereof, and the use of pp4
EP0279459A2 (fr) * 1987-02-20 1988-08-24 Kowa Company, Ltd. DNA codant pour un polypeptide anticoagulant
EP0279459A3 (en) * 1987-02-20 1990-06-27 Kowa Company, Ltd. Anticoagulant polypeptide
EP0318703A1 (fr) * 1987-11-03 1989-06-07 BEHRINGWERKE Aktiengesellschaft Préparation par génie génétique de la protéine anticoagulante PP4

Also Published As

Publication number Publication date
EP0217341B2 (fr) 1997-12-17
EP0217341A3 (en) 1988-04-06
EP0217341B1 (fr) 1990-12-12
JPH0710880B2 (ja) 1995-02-08
ES2001707A6 (es) 1988-06-01
KR870002848A (ko) 1987-04-13
JPS62174023A (ja) 1987-07-30
DE3676148D1 (de) 1991-01-24
US4732891A (en) 1988-03-22
KR940003751B1 (ko) 1994-04-30
CA1265446A (fr) 1990-02-06

Similar Documents

Publication Publication Date Title
EP0217341B1 (fr) Procédé pour la préparation d'une substance anticoagulante
Mammen et al. Activation of purified prothrombin to autoprothrombin I or autoprothrombin II (platelet cofactor II or autoprothrombin II-A)
JP3441456B2 (ja) 組織因子に基づくプロトロンビン時間試薬
FI57421B (fi) Foerfarande foer framstaellning av ett preparat ur maenniskoblodplasma med blodets koagulation befraemjande verkan
Marchesi et al. Studies on the purification and characterization of human factor VIII
JPH0780912B2 (ja) 血液凝固抑止性タンパク質
IE46754B1 (en) A glycoprotein and process for the production thereof
Baseler et al. Purification of haptoglobin and its effects on lymphocyte and alveolar macrophage responses
Molnar et al. The role of an α2-macroglobulin of rat serum in the phagocytosis of colloidal particles
CA1196281A (fr) Stabilisation des proteines plasmatiques par la chaleur
US3574818A (en) Diagnostic for rheumatism
Lawrence et al. The action of calf rennet and other proteolytic enzymes on κ-casein
Aoki et al. The procoagulant in human urine: Purification, assay and some biochemical and physiological properties
CA1198672A (fr) Agent fibrinolytique et methode de preparation
Bloom et al. Factor VIII (antihaemophilic factor) in tissues detected by antibody neutralization
US4873222A (en) Placenta-derived anticoagulating substance
Hanicki et al. Middle-sized ninhydrin-positive molecules in uraemic patients treated by repeated haemodialysis I. Preliminary characteristics
Arakawa et al. Purification of human angiotensin
Maki et al. Inhibitors of platelet aggregation and blood coagulation isolated from the human placenta
EP0041174B1 (fr) Produit activant la coagulation du sang et procédé pour sa préparation
Ganten Studies on the existence of an independent brain renin-angiotensin system: a model for extrarenal tissue renin.
Houdebine et al. Absence of mRNA for casein in free polysomes of lactating ewe mammary gland
Matsuda et al. Purification and characterization of procoagulant in human urine
Perricone et al. Activation of human complement by crude and purified antigens from Echinococcus granulosus
Baig et al. Purification and chemical characterization of salt-extractable glycoproteins from porcine mitral valve

Legal Events

Date Code Title Description
PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

AK Designated contracting states

Kind code of ref document: A2

Designated state(s): DE FR GB IT NL SE

PUAL Search report despatched

Free format text: ORIGINAL CODE: 0009013

AK Designated contracting states

Kind code of ref document: A3

Designated state(s): DE FR GB IT NL SE

17P Request for examination filed

Effective date: 19880829

17Q First examination report despatched

Effective date: 19890530

GRAA (expected) grant

Free format text: ORIGINAL CODE: 0009210

AK Designated contracting states

Kind code of ref document: B1

Designated state(s): DE FR GB IT NL SE

ET Fr: translation filed
REF Corresponds to:

Ref document number: 3676148

Country of ref document: DE

Date of ref document: 19910124

ITF It: translation for a ep patent filed
PLBI Opposition filed

Free format text: ORIGINAL CODE: 0009260

PLBI Opposition filed

Free format text: ORIGINAL CODE: 0009260

26 Opposition filed

Opponent name: BEHRINGWERKE AKTIENGESELLSCHAFT

Effective date: 19910814

26 Opposition filed

Opponent name: BOEHRINGER INGELHEIM GMBH, BINGEN

Effective date: 19910830

Opponent name: BEHRINGWERKE AKTIENGESELLSCHAFT

Effective date: 19910814

NLR1 Nl: opposition has been filed with the epo

Opponent name: BOEHRINGER INGELHEIM GMBH

Opponent name: BEHRINGWERKE AG.

ITTA It: last paid annual fee
EAL Se: european patent in force in sweden

Ref document number: 86113375.9

PLAW Interlocutory decision in opposition

Free format text: ORIGINAL CODE: EPIDOS IDOP

PLAW Interlocutory decision in opposition

Free format text: ORIGINAL CODE: EPIDOS IDOP

PLAW Interlocutory decision in opposition

Free format text: ORIGINAL CODE: EPIDOS IDOP

PUAH Patent maintained in amended form

Free format text: ORIGINAL CODE: 0009272

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: PATENT MAINTAINED AS AMENDED

27A Patent maintained in amended form

Effective date: 19971217

AK Designated contracting states

Kind code of ref document: B2

Designated state(s): DE FR GB IT NL SE

ET3 Fr: translation filed ** decision concerning opposition
ITF It: translation for a ep patent filed
NLR2 Nl: decision of opposition
NLR3 Nl: receipt of modified translations in the netherlands language after an opposition procedure
REG Reference to a national code

Ref country code: GB

Ref legal event code: IF02

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: DE

Payment date: 20050906

Year of fee payment: 20

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: IT

Payment date: 20050909

Year of fee payment: 20

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: SE

Payment date: 20050916

Year of fee payment: 20

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: GB

Payment date: 20050919

Year of fee payment: 20

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: FR

Payment date: 20050926

Year of fee payment: 20

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: NL

Payment date: 20050930

Year of fee payment: 20

REG Reference to a national code

Ref country code: GB

Ref legal event code: PE20

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: GB

Free format text: LAPSE BECAUSE OF EXPIRATION OF PROTECTION

Effective date: 20060928

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: NL

Free format text: LAPSE BECAUSE OF EXPIRATION OF PROTECTION

Effective date: 20060929

EUG Se: european patent has lapsed
NLV7 Nl: ceased due to reaching the maximum lifetime of a patent

Effective date: 20060929