EP0017127A2 - Verfahren zum Fraktionieren von Blut und Trennvorrichtung hierfür - Google Patents
Verfahren zum Fraktionieren von Blut und Trennvorrichtung hierfür Download PDFInfo
- Publication number
- EP0017127A2 EP0017127A2 EP80101547A EP80101547A EP0017127A2 EP 0017127 A2 EP0017127 A2 EP 0017127A2 EP 80101547 A EP80101547 A EP 80101547A EP 80101547 A EP80101547 A EP 80101547A EP 0017127 A2 EP0017127 A2 EP 0017127A2
- Authority
- EP
- European Patent Office
- Prior art keywords
- blood
- porous member
- elastic porous
- barrier
- collecting tube
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 230000004888 barrier function Effects 0.000 title claims abstract description 106
- 238000000034 method Methods 0.000 title claims abstract description 21
- 210000004369 blood Anatomy 0.000 title claims description 42
- 239000008280 blood Substances 0.000 title claims description 42
- 210000002966 serum Anatomy 0.000 claims abstract description 59
- 239000011148 porous material Substances 0.000 claims abstract description 22
- 230000005484 gravity Effects 0.000 claims description 25
- 239000007787 solid Substances 0.000 claims description 22
- 238000005119 centrifugation Methods 0.000 claims description 21
- 210000000601 blood cell Anatomy 0.000 claims description 14
- 229920003023 plastic Polymers 0.000 claims description 9
- 239000004033 plastic Substances 0.000 claims description 9
- 230000002093 peripheral effect Effects 0.000 claims description 8
- 239000002985 plastic film Substances 0.000 claims description 4
- 239000002984 plastic foam Substances 0.000 claims 1
- 102000009123 Fibrin Human genes 0.000 description 12
- 108010073385 Fibrin Proteins 0.000 description 12
- BWGVNKXGVNDBDI-UHFFFAOYSA-N Fibrin monomer Chemical compound CNC(=O)CNC(=O)CN BWGVNKXGVNDBDI-UHFFFAOYSA-N 0.000 description 12
- 229950003499 fibrin Drugs 0.000 description 12
- 239000000463 material Substances 0.000 description 10
- 229920005830 Polyurethane Foam Polymers 0.000 description 6
- 238000010908 decantation Methods 0.000 description 6
- 239000011496 polyurethane foam Substances 0.000 description 6
- 229920000915 polyvinyl chloride Polymers 0.000 description 6
- 239000004800 polyvinyl chloride Substances 0.000 description 6
- 238000004519 manufacturing process Methods 0.000 description 5
- 238000012986 modification Methods 0.000 description 5
- 230000004048 modification Effects 0.000 description 5
- 229920001971 elastomer Polymers 0.000 description 4
- 238000000926 separation method Methods 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 208000007536 Thrombosis Diseases 0.000 description 3
- 230000001413 cellular effect Effects 0.000 description 3
- 238000010276 construction Methods 0.000 description 3
- 239000006260 foam Substances 0.000 description 3
- 238000002156 mixing Methods 0.000 description 3
- 239000012071 phase Substances 0.000 description 3
- 229920000728 polyester Polymers 0.000 description 3
- 239000004677 Nylon Substances 0.000 description 2
- 238000004026 adhesive bonding Methods 0.000 description 2
- 239000000470 constituent Substances 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 229920001778 nylon Polymers 0.000 description 2
- 239000004417 polycarbonate Substances 0.000 description 2
- 229920000098 polyolefin Polymers 0.000 description 2
- 229920002635 polyurethane Polymers 0.000 description 2
- 239000004814 polyurethane Substances 0.000 description 2
- 238000003825 pressing Methods 0.000 description 2
- 229920005989 resin Polymers 0.000 description 2
- 239000011347 resin Substances 0.000 description 2
- 238000010998 test method Methods 0.000 description 2
- 206010018910 Haemolysis Diseases 0.000 description 1
- 239000004698 Polyethylene Substances 0.000 description 1
- 239000000853 adhesive Substances 0.000 description 1
- 230000001070 adhesive effect Effects 0.000 description 1
- 210000002457 barrier cell Anatomy 0.000 description 1
- 238000009534 blood test Methods 0.000 description 1
- 239000005038 ethylene vinyl acetate Substances 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 229920001821 foam rubber Polymers 0.000 description 1
- 238000005187 foaming Methods 0.000 description 1
- 230000004927 fusion Effects 0.000 description 1
- 230000008588 hemolysis Effects 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 229920000126 latex Polymers 0.000 description 1
- 239000004816 latex Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 229920001200 poly(ethylene-vinyl acetate) Polymers 0.000 description 1
- 229920000515 polycarbonate Polymers 0.000 description 1
- 229920001692 polycarbonate urethane Polymers 0.000 description 1
- -1 polyethylene Polymers 0.000 description 1
- 229920000573 polyethylene Polymers 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 229920001296 polysiloxane Polymers 0.000 description 1
- 229920005749 polyurethane resin Polymers 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicon dioxide Inorganic materials O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 239000000377 silicon dioxide Substances 0.000 description 1
- 229920002379 silicone rubber Polymers 0.000 description 1
- 239000004945 silicone rubber Substances 0.000 description 1
- 239000007790 solid phase Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Images
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5021—Test tubes specially adapted for centrifugation purposes
- B01L3/50215—Test tubes specially adapted for centrifugation purposes using a float to separate phases
Definitions
- This invention relates to a method for separating blood into a solid part including blood corpuscles and a liquid serum by centrifugation, and a barrier used for such method.
- blood is generally separated by centrifugation into serum and cellular solid matters such as blood corpuscles, and only the serum is collected for analysis and examination.
- serum and cellular solid matters such as blood corpuscles
- material such as gel material composed of silicone-silica which has an intermediate specific gravity between those of the serum and cellular solid matters is put in the test tube, the gel material is interposed between the serum and cellular matters by centrifugation, and the serum is separated by decantation. In this case, however, it is difficult to perfectly prevent fibrin and other solid matters from being mixed in the serum.
- a piston member in which a solid weight for specific gravity adjustment is coupled with a flexible filter member which is large enough to be in slidable contact with the inside wall of a blood-collecting tube, and having a specific gravity of from 1.03 to 1.09 as a whole is inserted in the blood-collecting tube (United States Patent No. 3,931,018).
- a specific gravity of from 1.03 to 1.09 as a whole is inserted in the blood-collecting tube
- the invention as claimed has been developed in consideration of the above circumstances, and is intended to provide a remedy by a method for separating blood and a device therefor capable of simplifying manufacture and reducing production cost without any possibility of causing blood cells, fibrin, and other solid matters to be mixed with serum.
- a method for separating blood collected in a blood-collecting tube into a serum part and a solid component part by centrifugation comprising the steps of introducing a barrier formed of an elastic porous member into the blood-collecting tube, the elastic porous member having porosity of 40 % or more, a continuous-pore size of 50 to 400 ⁇ , an overall true specific gravity greater than that of the serum part,and a larger cross-section in at least part thereof and perpendicular to the axial direction thereof than that of the blood-collecting tube; moving the elastic porous member to the interface between a , serum part layer and a solid component layer in the blood by centrifugal force produced in centrifuging the blood, and separating the serum in the blood.
- a barrier for centrifugation of blood which comprises an elastic porous member having porosity of 40 % or more, a continuous-pore size of 50 to 400 p, an overall true specific gravity greater than that of serum, and, at least at a part thereof, a cross-section a little larger than that of a blood-collecting tube.
- a unique point of this invention resides in that an elastic member with continuous pores of a specified size is used directly singly or substantially singly as a phase separator (or barrier).
- a phase separator or barrier
- Another peculiar point of the invention is that, although the true specific gravity of the barrier formed of such elastic member need be greater than that of serum, it need not always be smaller than that of the solid-phase part of the blood in separating the serum, unless hemolysis is caused. This may be attributed to the fact that the whole or principal part of the barrier of the invention, being a porous member, has extremely small mass (e.G. 100 to 300 mg).
- the idea of this invention is quite novel and may greatly widen the variety of available materials.
- the elastic porous member constituting at least the principal part of the barrier of the invention may be formed of elastic plastics foam, such as polyurethane foam, rubber foam (e.g. silicone rubber latex), polyvinyl chloride foam, polyformal resin, etc., having porosity of 40 % or more, preferably 97 to 98 %, and a continuous-pore size of 50 to 400 ⁇ , preferably 250 to 400 ⁇ . If porosity and pore size are smaller than those as specified, the isolation of the serum would be obtained in the ordinary centrifugal operation of 1000 1200 G for 10 minutes. A pore size of more than 400 ⁇ , is not desirable, since blood corpuscles would pass through a foam of such a large pore size, thereby contaminating the serum phase obtained. 1
- elastic plastics foam such as polyurethane foam, rubber foam (e.g. silicone rubber latex), polyvinyl chloride foam, polyformal resin, etc.
- the 25% compressive hardness (JIS K-6401 Test Method established in 1974) of the barrier should preferably be 5 to 150 kg/cm 2 .
- the barrier of the invention should be hydrophilic by nature or be made hydrophilic by some treatment for hydrophilicity. Such hydrophilic property is preferred because it will enable the serum to quickly penetrate the pores when the barrier is brought in contact with the blood, thereby facilitating the movement of the barrier.
- Elastic porous non-woven cloth may also be useful as far as the pores thereof substantially meet the above conditions.
- the overall specific gravity of the barrier should preferably be adjusted to 1.2 or more, more preferably to from 1.2 to 1.4.
- the barrier may be of any shape as long as at least a part of the barrier has a cross-section a little larger than that of a blood-collecting tube for centrifugation used with the barrier, so that the outer periphery of the large-diameter portion of the barrier may rub against the inside wall of the tube during centrifugation.
- a single elastic porous member can be directly used for the barrier.
- the outer peripheral portion of the barrier may be coated with silicone, or two or more elastic porous members may be combined with one another or with other r terials.
- a tube member with the outside diameter somewhat smaller than the inside diameter of the blood-collecting tube used e.g. a plastic.
- the tube may be fitted on the lower peripheral surface of a columnar or cylindrical barrier so as to reduce the area of contact and hence the frictional resistance between the barrier and the inside wall of the blood-collecting tube, thereby facilitating the sliding movement of the barrier during centrifugation.
- the specific gravity of the combination of the elastic porous member and the tube member need be greater than that of serum.
- the tube member may be formed of any thermally contractive material, such as polyolefin, polyvinyl chloride, nylon, polyester, polycarbonate, polyurethane or ethylenevinyl acetate copolymer.
- a columnar elastic porous member in the form of e.g. a truncated cone which has cross-sections substantially larger and smaller than that of the interior of the blood-collecting tube used, at its upper and lower portion, respectively, and is bottomed with a solid or porous hard layer.
- the hard layer may be formed by impregnating relatively hard plastic into the bottom portion of the porous member and solidifying the plastic, or by glueing a solid or porous,relatively hard plastic sheet to the bottom portion.
- the barrier of such construction exhibits extremely large deformation resistance during centrifugation, so that it may be prevented from turning sideways or being distorted while sliding down the tube thereby ensuring the descending movement of the barrier in a properly erected state during centrifugation.
- the shape of the final product may be obtained directly by stamping out a truncated-cone- shaped member after glueing a hard plastic sheet to one side of an elastic porous sheet or after impregnating a solution of hard plastic into the porous sheet to a predetermined thickness, so that the manufacture of the barrier may be simplified substantially, so as to permit for reduction in production cost.
- the porous member may be joined with the tube member, hard plastic sheet or the like by using adhesives, heat sealing or any other suitable means.
- the barrier is introduced into the blood-collecting tube before or after collecting the blood, the blood is centrifuged, and then the serum part is easily separated by decantation.
- FIGs 1(A) to 1(C) show processes of centrifuging blood serum by using the blood separator according to the invention.
- whole blood 2 is collected in a blood-collecting tube 1
- a barrier 3 formed of an elastic porous member is fitted in the opening of the tube 1
- the tube 1 is set in a centrifugal separator for centrifugation.
- the barrier 3 is caused gradually to slide down the inside wall of the blood-collecting tube 1 toward the bottom of the tube 1 by centrifugal force, as shown in Figure 1(B).
- the bottom end of the barrier 3 touches the surface of the blood 2, the serum is caused to penetrate into pores of the barrier 3 by capillarity.
- the pores of the barrier 3 are substantially filled with the serum, and the barrier 3 is further moved down until it is finally held substantially midway between a serum layer 4 and a solid component layer 5.
- solid constituents such as blood corpuscles and fibrin are trapped in the pores of the barrier 3 and will never be mixed with the serum. This is ensured because the solid constituents are retained in the continuous pores of the barrier 3 the framework of which has a complicated three-dimensional structure.
- the barrier 3 slides relatively slowly down the inside wall of the blood-collecting tube 1 by its elasticity, so that blood corpuscles, fibrin, etc. stuck to the inside wall can be cleared or swept away substantially thoroughly. As a result, there may be obtained serum which does not contain blood corpuscles, fibrin or any other solid matters.
- the barrier 3 stopped at the interfacial position sticks fast to the inside wall of the blood-collecting tube 1 by its own elasticity, pressing against the inside wall, so that only the serum part can be separated by decantation.
- the barrier of this invention may be inserted into the blood-collecting tube during centrifugation after blood collection, as in the case of the above embodiment, or otherwise be held in the tube beforehand.
- Figure 2 shows an example of the latter case.
- a barrier 23 having an annular hard layer 27 on its bottom is held by a rubber stopper 24 within a vacuum blood-collecting tube 21 the inside of which is kept at a vacuum. That is, the rubber stopper 24 has a cavity 25 in the lower end, while the barrier 23 has on its top a truncated cone-spaped projection 28 with the outside diameter larger than the diameter of the cavity 25.
- the projection 25 is fitted and held in the cavity 25 so that the barrier 23 will not be removed from the rubber stopper 24 if the stopper 24 is pierced with a needle for blood collection.
- a barrier may be fixed to one end of a blood-collecting tube sealed with a rubber stopper at each end, the one end being opposite to the blood intake side of the tube.
- Figures 3 to 16 illustrate the respective shapes of several modifications of the barrier.
- a columnar barrier 31 (Fig. 3) with or without one or more annular flanges along the peripheral surface thereof; a barrier 42 (Fig. 4) with a pair of parallel annular flanges 41; a barrier 52 (Fig. 5) similar to the columnar barrier of Fig. 3 but with a cavity 51 on one side thereof; a barrier 62 (Fig. 6) similar to the barrier of Figure 4 but with the same cavity 51 of Figure 5; a barrier 72 (Fig. 7) formed of a column with flanges 71 at the top and bottom thereof; a barrier (Fig. 8) of the same structure of Figure 7 but with the cavity 51; a barrier 82 (Fig.
- a barrier (Fig. 10) of the same structure of Figure 9 but with the cavity 51; a spherical barrier 92 (Fig. 11); a barrier (Fig. 12) of the same structure of Figure 11 but with the cavity 51; a barrier formed by fitting a small-diameter tube member 100 on the lower peripheral surface of the columnar porous member 31 as shown in Figure 13(A) to restrict the lower portion of the porous member 31 as shown in Figure 13(B) so as to reduce the area of contact with the blood-collecting tube; a barrier (Fig. 10) of the same structure of Figure 9 but with the cavity 51; a spherical barrier 92 (Fig. 11); a barrier (Fig. 12) of the same structure of Figure 11 but with the cavity 51; a barrier formed by fitting a small-diameter tube member 100 on the lower peripheral surface of the columnar porous member 31 as shown in Figure 13(A) to restrict the lower portion of the porous member 31 as shown in Figure 13(B) so as to reduce the area of contact with the
- a barrier 112 formed by bonding a hard layer 11 to one small-diameter end of an elastic porous member 110 substantially in the form of a truncated cone as shown in Figures 15 and 16.
- Available materials for the hard layer 111 include plastics such as polyolefin, polyvinyl chloride, nylon, polyester, polycarbonate, and polyurethane, fluorine- contained polymers and other organic and inorganic substances.
- hard layer may be porous such as mesh-like.
- the thickness of the hard layer preferably ranges from 0.1 mm to 5.0 mm, and more preferably from 0.1 mm to 1.0 mm.
- the barrier shape may lend itself to various modifications.
- the barrier should have porosity, pore size, and apparent or real specific gravity within prescribed ranges, and be of such suitable size that it may rub against the inside wall of the blood-collecting tube when it slides thereon during centrifugation.
- the barrier being a simple elastic porous member with or without a plastic tube member or a hard layer attached thereto, is so simple in construction that it can be manufactured very easily at reasonable cost. Since the elastic porous member transmits only the serum to be separated, there may be obtained pure serum containing no solid matters such as blood corpuscles and fibrin.
- a test for separating serum from blood was conducted by using the barrier 52 shown in Figure 5.
- Polyurethane foam with a porosity of 98 %, a pore size of 300 ⁇ , a true specific gravity of 1.2, a 25-% compressive hardness (based on JIS K-6401 Test Method) of 20 kg/cm 2 , and a number of barrier cells of approximately 75/25 mm was used for the barrier. Since the framework of the polyurethane foam has continuous pores of complicated three-dimensional structure and reduces the passage resistance of serum, it had previously been removed by thermally dissolving filmy material formed around the pores at foaming, as described in Japanese Patent Publication No. 752/66 (January 25, 1966), U.S. Application Nos. 203,603 (March 7, 1963), 271,031 (April 5, 1963), 294,861 (July 15, 1963) and 347,246 (February 25, 1964).
- the barrier measured 13.7 mm in diameter, 12 mm in height, 4 mm between the center of its top and the peak of the cavity 51, and 2 mm in the thickness of its peripheral wall defining the cavity 51 at the lower portion.
- the blood-collecting tube used had an inside diameter of 13.6 mm and accommodated 10 ml of blood.
- the barrier 52 of such construction was inserted into the upper portion of the blood-collecting tube which hsd been left at normal temperature for approximately 60 minutes after collecting blood, and then centrifugation was performed by using a centrifugal separator for 10 minutes with the centrifugal force at the central portion of the tube set at approximately 1,200 G (approx 1,000 G at the barrier top).
- the barrier 52 was located midway between a blood clot and serum, pressing its cavity 51 against the top of the blood clot. Observation of the blood-collecting tube by the naked eye revealed hardly any fibrin or blood corpuscles in the serum, which held true after the serum was transferred to another vessel by decantation. Moreover, it was found that the suspended blood corpuscles and fibrin near the surface of the blood clot remained trapped in the continuous pores of the barrier. The yield of the serum collected in this manner proved to be approximately 4.5 ml - substantially the whole quantity of serum separated.
- the barrier 31 shown in Figure 13 was manufactured by using the same polyurethane foam of Example 1. In this case, however, the barrier 31 hat no cavity, and the tube 100 of 3 mm height, 12.2 mm inside diameter and 13.0 mm outside diameter was fitted on the lower portion of the columnar porous member 31 (polyurethane foam) of 13.7 mm diameter and 12 mm height.
- the tube 100 was made of polyethylene, and was provided at the bottom end with an abutment portion (not shown) to engage the bottom end of the porous member 31.
- This barrier was inserted through the opening of the blood-collecting tube (the same one as Example 1) containing blood, which had been kept at normal temperature for 60 minutes, to a depth where the barrier touched the blood surface. After leaving the barrier to stand for a while, centrifugation was carried out under normal conditions so that the centrifugal force at the central portion of the blood-collecting tube might become approximately 11200 G.
- decantation caused neither shifting of the barrier nor mixing of blood corpuscles or fibrin.
- the outside diameter of the tube 100 was smaller than the inside diameter of the blood-collecting tube, and the upper side wall of the porous member 31 was so designed as to form a slope. Therefore, the barrier touched the inside wall of the blood-collecting tube only at the opening portion thereof when it was fitted in the tube. Consequently, the barrier was never prevented from descending by the viscosity of blood sticking to the upper portion of the inside wall of the blood-collecting tube after being left to stand for a while.
- the barrier shown in Figure 14 was manufactured to obtain the same effect as the barrier of Example 2 and to maximize the yield of serum.
- the porous member 31 used was just the same as the porous member used in Example 2 in material, dimensions and shape, except that it was provided with the cavity 51 defined therein at the lower portion.
- the tube 100 made of thermally contractive polyvinyl chloride was fitted on the lower portion of the porous member 31.
- the tube 100 measured about 13ji in thickness, 12.0 mm in outside diameter, and 6 mm in height when it was fitted on the porous member 31.
- the bottom end of the tube 100 and the bottom joint part of the porous member 31 were bonded together at several portions by thermal fusion.
- Serum separation was concucted in the same manner as Example 1 by using the barrier 112 consisting of the elastic porous member 110 which is formed of the same polyurethane foam of Example 1 and has the form of a truncated cone as shown in Figures 15 and 16, measuring 15.5 mm in diameter across the upper large-diameter section, 12.8 mm in diameter across the lower small-diameter section, and 9 mm in height, and the hard layer 111 which is formed of a hard polyvinyl chloride film of 200 ⁇ thickness bonded to the bottom face of the porous member 110.
- the barrier 112 consisting of the elastic porous member 110 which is formed of the same polyurethane foam of Example 1 and has the form of a truncated cone as shown in Figures 15 and 16, measuring 15.5 mm in diameter across the upper large-diameter section, 12.8 mm in diameter across the lower small-diameter section, and 9 mm in height, and the hard layer 111 which is formed of a hard polyvinyl
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- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Hematology (AREA)
- Clinical Laboratory Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
AT80101547T ATE11378T1 (de) | 1979-03-23 | 1980-03-24 | Verfahren zum fraktionieren von blut und trennvorrichtung hierfuer. |
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP33943/79 | 1979-03-23 | ||
JP54033943A JPS5917386B2 (ja) | 1979-03-23 | 1979-03-23 | 血液分離方法および装置 |
JP3521580A JPS56130656A (en) | 1980-03-19 | 1980-03-19 | Barrier for blood centrifugation |
JP35215/80 | 1980-03-19 |
Publications (3)
Publication Number | Publication Date |
---|---|
EP0017127A2 true EP0017127A2 (de) | 1980-10-15 |
EP0017127A3 EP0017127A3 (en) | 1980-12-10 |
EP0017127B1 EP0017127B1 (de) | 1985-01-23 |
Family
ID=26372715
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP19800101547 Expired EP0017127B1 (de) | 1979-03-23 | 1980-03-24 | Verfahren zum Fraktionieren von Blut und Trennvorrichtung hierfür |
Country Status (3)
Country | Link |
---|---|
EP (1) | EP0017127B1 (de) |
AU (1) | AU542204B2 (de) |
DE (1) | DE3069996D1 (de) |
Cited By (20)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0040022A2 (de) * | 1980-05-12 | 1981-11-18 | American Hospital Supply Corporation | Filterelement für eine Vorrichtung zum Abtrennen von Serum |
FR2536671A1 (fr) * | 1982-11-26 | 1984-06-01 | Sartorius Gmbh | Appareil de filtrage pour liquides, de type statique a membrane |
EP0638804A1 (de) * | 1993-08-13 | 1995-02-15 | Niigata Chemicals And Plastics Co. Ltd | Vorrichtung zum Abtrennen von Serum |
DE19542225A1 (de) * | 1995-11-01 | 1997-05-07 | Buero Fuer Biomedizinische Ing | Verfahren und Vorrichtung zur Bestimmung von rheologischen und mechanischen Stoffkenngrößen |
US6280400B1 (en) | 1998-12-05 | 2001-08-28 | Becton Dickinson And Company | Device and method for separating component of a liquid sample |
US6406671B1 (en) | 1998-12-05 | 2002-06-18 | Becton, Dickinson And Company | Device and method for separating components of a fluid sample |
US6409528B1 (en) | 1999-12-06 | 2002-06-25 | Becton, Dickinson And Company | Device and method for collecting, preparation and stabilizing a sample |
US6465256B1 (en) | 2000-08-26 | 2002-10-15 | Becton, Dickinson And Company | Device and method for separating components of a fluid sample |
US6471069B2 (en) | 1999-12-03 | 2002-10-29 | Becton Dickinson And Company | Device for separating components of a fluid sample |
US6497325B1 (en) | 1998-12-05 | 2002-12-24 | Becton Dickinson And Company | Device for separating components of a fluid sample |
US6516953B1 (en) | 1998-12-05 | 2003-02-11 | Becton, Dickinson And Company | Device for separating components of a fluid sample |
US6537503B1 (en) | 1999-12-03 | 2003-03-25 | Becton Dickinson And Company | Device and method for separating components of a fluid sample |
US6803022B2 (en) | 1999-12-06 | 2004-10-12 | Becton, Dickinson And Company | Device and method for separating components of a fluid sample |
EP2680976A2 (de) * | 2011-03-02 | 2014-01-08 | Rarecyte, Inc. | Rohr und schwimmersysteme für dichtebasierte fluidtrennung |
WO2015070273A1 (de) * | 2013-11-14 | 2015-05-21 | Greiner Bio-One Gmbh | Aufnahmevorrichtung, verfahren zum bereitstellen derselben sowie verfahren zum trennen eines gemisches |
US9339741B2 (en) | 2008-07-21 | 2016-05-17 | Becton, Dickinson And Company | Density phase separation device |
WO2018197562A1 (de) * | 2017-04-26 | 2018-11-01 | Sarstedt Aktiengesellschaft & Co.Kg | Trennkörper |
US10343157B2 (en) | 2009-05-15 | 2019-07-09 | Becton, Dickinson And Company | Density phase separation device |
US11241685B2 (en) | 2017-04-26 | 2022-02-08 | Sarstedt Aktiengesellschaft & Co. Kg | Separator |
US11273440B2 (en) | 2017-04-26 | 2022-03-15 | Sarstedt Aktiengesellschaft & Co. Kg | Separator |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7947236B2 (en) | 1999-12-03 | 2011-05-24 | Becton, Dickinson And Company | Device for separating components of a fluid sample |
US9694359B2 (en) | 2014-11-13 | 2017-07-04 | Becton, Dickinson And Company | Mechanical separator for a biological fluid |
CN113440927B (zh) * | 2020-03-27 | 2022-05-20 | 张文杰 | 乳糜化脂肪水溶性成分提取装置与方法 |
Citations (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3300051A (en) * | 1963-09-26 | 1967-01-24 | Internat Equipment Co | Filter tube for use in a centrifuge |
US3786985A (en) * | 1973-01-05 | 1974-01-22 | Hoffmann La Roche | Blood collection container |
US3870639A (en) * | 1974-01-02 | 1975-03-11 | Moore Perk Corp | Filtering device |
US3894950A (en) * | 1974-02-27 | 1975-07-15 | Becton Dickinson Co | Serum separator improvement with stretchable filter diaphragm |
US3901219A (en) * | 1974-07-25 | 1975-08-26 | Becton Dickinson Co | Blood collecting container and method |
US3931018A (en) * | 1974-08-09 | 1976-01-06 | Becton, Dickinson And Company | Assembly for collection, separation and filtration of blood |
US3960727A (en) * | 1974-08-09 | 1976-06-01 | Hochstrasser Harry T | Apparatus and method for isolating soluble blood components |
US3972812A (en) * | 1975-05-08 | 1976-08-03 | Becton, Dickinson And Company | Blood serum separation filter disc |
DE2743407A1 (de) * | 1976-09-30 | 1978-04-06 | Ken Heimreid | Verfahren und vorrichtung zur untersuchung von blut |
-
1980
- 1980-03-24 AU AU56786/80A patent/AU542204B2/en not_active Ceased
- 1980-03-24 DE DE8080101547T patent/DE3069996D1/de not_active Expired
- 1980-03-24 EP EP19800101547 patent/EP0017127B1/de not_active Expired
Patent Citations (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3300051A (en) * | 1963-09-26 | 1967-01-24 | Internat Equipment Co | Filter tube for use in a centrifuge |
US3786985A (en) * | 1973-01-05 | 1974-01-22 | Hoffmann La Roche | Blood collection container |
US3870639A (en) * | 1974-01-02 | 1975-03-11 | Moore Perk Corp | Filtering device |
US3894950A (en) * | 1974-02-27 | 1975-07-15 | Becton Dickinson Co | Serum separator improvement with stretchable filter diaphragm |
US3901219A (en) * | 1974-07-25 | 1975-08-26 | Becton Dickinson Co | Blood collecting container and method |
US3931018A (en) * | 1974-08-09 | 1976-01-06 | Becton, Dickinson And Company | Assembly for collection, separation and filtration of blood |
US3960727A (en) * | 1974-08-09 | 1976-06-01 | Hochstrasser Harry T | Apparatus and method for isolating soluble blood components |
US3972812A (en) * | 1975-05-08 | 1976-08-03 | Becton, Dickinson And Company | Blood serum separation filter disc |
DE2743407A1 (de) * | 1976-09-30 | 1978-04-06 | Ken Heimreid | Verfahren und vorrichtung zur untersuchung von blut |
Cited By (32)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0040022A3 (de) * | 1980-05-12 | 1982-09-08 | American Hospital Supply Corporation | Filterelement für eine Vorrichtung zum Abtrennen von Serum |
EP0040022A2 (de) * | 1980-05-12 | 1981-11-18 | American Hospital Supply Corporation | Filterelement für eine Vorrichtung zum Abtrennen von Serum |
FR2536671A1 (fr) * | 1982-11-26 | 1984-06-01 | Sartorius Gmbh | Appareil de filtrage pour liquides, de type statique a membrane |
US5632895A (en) * | 1993-08-13 | 1997-05-27 | Nigata Engineering Co., Ltd. | Serum separating device and apparatus for serum separation |
EP0638804A1 (de) * | 1993-08-13 | 1995-02-15 | Niigata Chemicals And Plastics Co. Ltd | Vorrichtung zum Abtrennen von Serum |
DE19542225B4 (de) * | 1995-11-01 | 2011-05-26 | L.U.M. Gmbh | Verfahren und Vorrichtung zur Bestimmung von rheologischen und mechanischen Stoffkenngrößen |
DE19542225A1 (de) * | 1995-11-01 | 1997-05-07 | Buero Fuer Biomedizinische Ing | Verfahren und Vorrichtung zur Bestimmung von rheologischen und mechanischen Stoffkenngrößen |
US6280400B1 (en) | 1998-12-05 | 2001-08-28 | Becton Dickinson And Company | Device and method for separating component of a liquid sample |
US6406671B1 (en) | 1998-12-05 | 2002-06-18 | Becton, Dickinson And Company | Device and method for separating components of a fluid sample |
US7153477B2 (en) | 1998-12-05 | 2006-12-26 | Becton Dickinson And Company | Device and method for separating components of a fluid sample |
US6497325B1 (en) | 1998-12-05 | 2002-12-24 | Becton Dickinson And Company | Device for separating components of a fluid sample |
US6516953B1 (en) | 1998-12-05 | 2003-02-11 | Becton, Dickinson And Company | Device for separating components of a fluid sample |
US6471069B2 (en) | 1999-12-03 | 2002-10-29 | Becton Dickinson And Company | Device for separating components of a fluid sample |
US6537503B1 (en) | 1999-12-03 | 2003-03-25 | Becton Dickinson And Company | Device and method for separating components of a fluid sample |
US6409528B1 (en) | 1999-12-06 | 2002-06-25 | Becton, Dickinson And Company | Device and method for collecting, preparation and stabilizing a sample |
US6803022B2 (en) | 1999-12-06 | 2004-10-12 | Becton, Dickinson And Company | Device and method for separating components of a fluid sample |
US6465256B1 (en) | 2000-08-26 | 2002-10-15 | Becton, Dickinson And Company | Device and method for separating components of a fluid sample |
US9339741B2 (en) | 2008-07-21 | 2016-05-17 | Becton, Dickinson And Company | Density phase separation device |
US10343157B2 (en) | 2009-05-15 | 2019-07-09 | Becton, Dickinson And Company | Density phase separation device |
US10456782B2 (en) | 2009-05-15 | 2019-10-29 | Becton, Dickinson And Company | Density phase separation device |
US10413898B2 (en) | 2009-05-15 | 2019-09-17 | Becton, Dickinson And Company | Density phase separation device |
US10376879B2 (en) | 2009-05-15 | 2019-08-13 | Becton, Dickinson And Company | Density phase separation device |
EP2680976A2 (de) * | 2011-03-02 | 2014-01-08 | Rarecyte, Inc. | Rohr und schwimmersysteme für dichtebasierte fluidtrennung |
EP2680976A4 (de) * | 2011-03-02 | 2014-08-20 | Rarecyte Inc | Rohr und schwimmersysteme für dichtebasierte fluidtrennung |
WO2015070273A1 (de) * | 2013-11-14 | 2015-05-21 | Greiner Bio-One Gmbh | Aufnahmevorrichtung, verfahren zum bereitstellen derselben sowie verfahren zum trennen eines gemisches |
US20160262678A1 (en) * | 2013-11-14 | 2016-09-15 | Greiner Bio-One Gmbh | Receptacle device, method for providing the same and method for separating a mixture |
CN105848580A (zh) * | 2013-11-14 | 2016-08-10 | 格莱恩比奥-奥内有限公司 | 容纳装置、用于提供所述容纳装置的方法以及用于分离混合物的方法 |
CN105848580B (zh) * | 2013-11-14 | 2019-12-20 | 格莱恩比奥-奥内有限公司 | 用于提供容纳装置的方法以及用于分离混合物的方法 |
WO2018197562A1 (de) * | 2017-04-26 | 2018-11-01 | Sarstedt Aktiengesellschaft & Co.Kg | Trennkörper |
US11241685B2 (en) | 2017-04-26 | 2022-02-08 | Sarstedt Aktiengesellschaft & Co. Kg | Separator |
US11241686B2 (en) | 2017-04-26 | 2022-02-08 | Sarstedt Aktiengesellschaft & Co.Kg | Separator |
US11273440B2 (en) | 2017-04-26 | 2022-03-15 | Sarstedt Aktiengesellschaft & Co. Kg | Separator |
Also Published As
Publication number | Publication date |
---|---|
AU542204B2 (en) | 1985-02-14 |
AU5678680A (en) | 1980-09-25 |
EP0017127B1 (de) | 1985-01-23 |
DE3069996D1 (en) | 1985-03-07 |
EP0017127A3 (en) | 1980-12-10 |
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