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EA202190969A1 - Новые белки, ингибирующие активность насекомых - Google Patents

Новые белки, ингибирующие активность насекомых

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Publication number
EA202190969A1
EA202190969A1 EA202190969A EA202190969A EA202190969A1 EA 202190969 A1 EA202190969 A1 EA 202190969A1 EA 202190969 A EA202190969 A EA 202190969A EA 202190969 A EA202190969 A EA 202190969A EA 202190969 A1 EA202190969 A1 EA 202190969A1
Authority
EA
Eurasian Patent Office
Prior art keywords
tic7941pl
proteins
disclosed
pesticidal
nucleic acid
Prior art date
Application number
EA202190969A
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English (en)
Inventor
Дэвид Дж. Боуэн
Кэтрин А. Чья
Даньци Чэнь
Тодд А. Цихе
Арлин Р. Хоу
Дженнифер Л. Лутке
Барбара И. Уиггинс
Юаньцзи Чжан
Original Assignee
Монсанто Текнолоджи ЛЛК
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Application filed by Монсанто Текнолоджи ЛЛК filed Critical Монсанто Текнолоджи ЛЛК
Publication of EA202190969A1 publication Critical patent/EA202190969A1/ru

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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
    • A01N63/25—Paenibacillus
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/12—Processes for modifying agronomic input traits, e.g. crop yield
    • A01H1/122—Processes for modifying agronomic input traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • A01H1/1245—Processes for modifying agronomic input traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, e.g. pathogen, pest or disease resistance
    • A01H1/127—Processes for modifying agronomic input traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, e.g. pathogen, pest or disease resistance for insect resistance
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A01H5/10—Seeds
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
    • A01N47/40—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides
    • A01N47/42—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides containing —N=CX2 groups, e.g. isothiourea
    • A01N47/44—Guanidine; Derivatives thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/60—Isolated nucleic acids
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P7/00—Arthropodicides
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    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
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    • C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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  • Life Sciences & Earth Sciences (AREA)
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Abstract

Раскрыты пестицидные белки, проявляющие токсическую активность против видов чешуекрылых вредителей, и они включают, но не ограничиваются ими, TIC7941, TIC7941PL_1, TIC7941PL_2 и TIC7941PL_3. Представлены конструкции ДНК, которые содержат последовательность рекомбинантной нуклеиновой кислоты, кодирующую один или несколько раскрытых пестицидных белков. Представлены трансгенные растения, растительные клетки, семена и части растений, устойчивые к заражению чешуекрылыми насекомыми, которые содержат последовательности рекомбинантных нуклеиновых кислот, кодирующие пестицидные белки по настоящему изобретению. Также представлены способы обнаружения присутствия последовательностей рекомбинантных нуклеиновых кислот или белков по настоящему изобретению в биологическом образце и способы борьбы с вредителями видов чешуекрылых с использованием любого из пестицидных белков TIC7941, TIC7941PL_1, TIC7941PL_2 и TIC7941PL_3. Также раскрыты способы и композиции для улучшения инсектицидной активности пестицидного белка против видов насекомых-вредителей. Кроме того, раскрыты способ и композиции для снижения экспрессии пестицидного белка в тканях органов размножения трансгенного растения.
EA202190969A 2019-01-22 2020-01-21 Новые белки, ингибирующие активность насекомых EA202190969A1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201962795066P 2019-01-22 2019-01-22
PCT/US2020/014437 WO2020154301A1 (en) 2019-01-22 2020-01-21 Novel insect inhibitory proteins

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US (2) US11266151B2 (ru)
EP (1) EP3914608A4 (ru)
KR (1) KR20210118055A (ru)
CN (2) CN119040350A (ru)
AR (1) AR117865A1 (ru)
AU (1) AU2020212955B2 (ru)
BR (1) BR112021006574A2 (ru)
CA (1) CA3115551A1 (ru)
CL (2) CL2021001055A1 (ru)
CO (1) CO2021006033A2 (ru)
CR (1) CR20210231A (ru)
EA (1) EA202190969A1 (ru)
MX (1) MX2021005363A (ru)
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UY38551A (es) 2020-08-31
CL2022003221A1 (es) 2023-04-21
CN112955555B (zh) 2024-09-03
BR112021006574A2 (pt) 2021-08-10
KR20210118055A (ko) 2021-09-29
AU2020212955B2 (en) 2025-01-02
CN112955555A (zh) 2021-06-11
CO2021006033A2 (es) 2021-05-20
AU2020212955A1 (en) 2021-05-06
EP3914608A1 (en) 2021-12-01
US12324437B2 (en) 2025-06-10
MX2021005363A (es) 2021-06-30
CL2021001055A1 (es) 2021-10-01
CN119040350A (zh) 2024-11-29
AR117865A1 (es) 2021-09-01
CA3115551A1 (en) 2020-07-30
CR20210231A (es) 2021-08-26
US20220248686A1 (en) 2022-08-11
EP3914608A4 (en) 2023-05-17
US11266151B2 (en) 2022-03-08
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