[go: up one dir, main page]

EA201890640A1 - RECOMBINANT VECTORS CONTAINING PEPTIDE 2A - Google Patents

RECOMBINANT VECTORS CONTAINING PEPTIDE 2A

Info

Publication number
EA201890640A1
EA201890640A1 EA201890640A EA201890640A EA201890640A1 EA 201890640 A1 EA201890640 A1 EA 201890640A1 EA 201890640 A EA201890640 A EA 201890640A EA 201890640 A EA201890640 A EA 201890640A EA 201890640 A1 EA201890640 A1 EA 201890640A1
Authority
EA
Eurasian Patent Office
Prior art keywords
vectors containing
recombinant vectors
containing peptide
peptide
vector
Prior art date
Application number
EA201890640A
Other languages
Russian (ru)
Inventor
Дуглас Дж. Джолли
Эми Х. Лин
Эндрю Хоуфекр
Дэниел Дж. Хоуган
Дерек Г. Остертег
Original Assignee
Токаджен Инк.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Токаджен Инк. filed Critical Токаджен Инк.
Publication of EA201890640A1 publication Critical patent/EA201890640A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • A61K48/005—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy characterised by an aspect of the 'active' part of the composition delivered, i.e. the nucleic acid delivered
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/53—Colony-stimulating factor [CSF]
    • C07K14/535—Granulocyte CSF; Granulocyte-macrophage CSF
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/555—Interferons [IFN]
    • C07K14/57—IFN-gamma
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70503—Immunoglobulin superfamily
    • C07K14/70507—CD2
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2803—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily
    • C07K16/2827—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily against B7 molecules, e.g. CD80, CD86
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/70—Immunoglobulins specific features characterized by effect upon binding to a cell or to an antigen
    • C07K2317/76—Antagonist effect on antigen, e.g. neutralization or inhibition of binding
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/30—Non-immunoglobulin-derived peptide or protein having an immunoglobulin constant or Fc region, or a fragment thereof, attached thereto
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/60—Fusion polypeptide containing spectroscopic/fluorescent detection, e.g. green fluorescent protein [GFP]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1138—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against receptors or cell surface proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering nucleic acids [NA]
    • C12N2310/141—MicroRNAs, miRNAs
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/13011—Gammaretrovirus, e.g. murine leukeamia virus
    • C12N2740/13021—Viruses as such, e.g. new isolates, mutants or their genomic sequences
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/13011—Gammaretrovirus, e.g. murine leukeamia virus
    • C12N2740/13022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/13011—Gammaretrovirus, e.g. murine leukeamia virus
    • C12N2740/13041—Use of virus, viral particle or viral elements as a vector
    • C12N2740/13043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
    • C12N2840/203—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Zoology (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Virology (AREA)
  • Epidemiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Настоящее изобретение относится к модифицированным рекомбинантным ретровирусам, содержащим последовательность, кодирующую пептид 2А или подобный ему пептид и функционально присоединенную к гетерологичному полинуклеотиду. Настоящее изобретение также относится к клеткам и к вектору, где эти клетки экспрессируют или содержат такой вектор, а также к способам применения указанных модифицированных векторов для лечения заболеваний и расстройств.The present invention relates to modified recombinant retroviruses containing a sequence encoding a peptide 2A or similar peptide and functionally attached to a heterologous polynucleotide. The present invention also relates to cells and a vector, where these cells express or contain such a vector, as well as methods of using said modified vectors for treating diseases and disorders.

EA201890640A 2015-09-04 2016-09-01 RECOMBINANT VECTORS CONTAINING PEPTIDE 2A EA201890640A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201562214884P 2015-09-04 2015-09-04
PCT/US2016/049947 WO2017040815A1 (en) 2015-09-04 2016-09-01 Recombinant vectors comprising 2a peptide

Publications (1)

Publication Number Publication Date
EA201890640A1 true EA201890640A1 (en) 2018-09-28

Family

ID=58188345

Family Applications (1)

Application Number Title Priority Date Filing Date
EA201890640A EA201890640A1 (en) 2015-09-04 2016-09-01 RECOMBINANT VECTORS CONTAINING PEPTIDE 2A

Country Status (13)

Country Link
US (1) US11279949B2 (en)
EP (1) EP3325633A4 (en)
JP (1) JP2018526007A (en)
KR (1) KR20180048743A (en)
CN (1) CN108138201A (en)
AU (1) AU2016317936A1 (en)
CA (1) CA2996797A1 (en)
EA (1) EA201890640A1 (en)
HK (1) HK1251612A1 (en)
IL (1) IL257819A (en)
MX (1) MX2018002744A (en)
PH (1) PH12018500477A1 (en)
WO (1) WO2017040815A1 (en)

Families Citing this family (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017040815A1 (en) * 2015-09-04 2017-03-09 Tocagen Inc. Recombinant vectors comprising 2a peptide
GB201710973D0 (en) 2017-07-07 2017-08-23 Avacta Life Sciences Ltd Scaffold proteins
IL275205B2 (en) 2017-12-15 2025-03-01 Flagship Pioneering Innovations Vi Llc Compositions comprising circular polyribonucleotides and uses thereof
AU2019276261A1 (en) * 2018-05-30 2020-12-03 Institute For Research In Biomedicine Engineering B lymphocytes by utilizing endogenous activation-induced cytidine deaminase
WO2019241549A1 (en) 2018-06-15 2019-12-19 A2 Biotherapeutics, Inc. Foxp3-expressing car-t regulatory cells
CN109022374B (en) * 2018-08-16 2021-05-14 华中农业大学 A kind of Seneca Valley virus recombinant plasmid, recombinant virus and construction method
KR102879284B1 (en) 2018-08-29 2025-10-31 난징 레전드 바이오테크 씨오., 엘티디. Anti-mesothelin chimeric antigen receptor (CAR) constructs and uses thereof
EP3880829A4 (en) * 2018-11-13 2022-08-24 Denovo Biopharma LLC Recombinant vectors comprising genes for binding domains and secretable peptides
CN119409834A (en) 2019-08-12 2025-02-11 北京恩瑞尼生物科技股份有限公司 Methods and compositions for promoting and enhancing T cell-mediated immune responses through ADCC targeting of CD39 expressing cells
CA3149557A1 (en) 2019-09-30 2021-04-08 Scott J. Balsitis Hbv vaccines and methods treating hbv
TW202128775A (en) 2019-10-16 2021-08-01 英商阿法克塔生命科學有限公司 Pd-l1 inhibitor - tgfβ inhibitor bispecific drug moieties
EP4149538A1 (en) * 2020-05-11 2023-03-22 Janssen Pharmaceuticals, Inc. Sars-cov-2 vaccines
US20230304031A1 (en) * 2020-05-11 2023-09-28 Abintus Bio, Inc. Vectors and methods for in vivo transduction
GB202101299D0 (en) 2020-06-09 2021-03-17 Avacta Life Sciences Ltd Diagnostic polypetides and methods
EP4196493A1 (en) 2020-08-13 2023-06-21 A2 Biotherapeutics, Inc. Gene fusions for control of genetically modified cells
KR102424707B1 (en) * 2020-10-12 2022-07-25 전북대학교산학협력단 Recombinant vector expressing multiple antigens in Eukaryote cytosol and an attenuated Salmonella Typhimurium as the vector delivery system to host cells
WO2022234003A1 (en) 2021-05-07 2022-11-10 Avacta Life Sciences Limited Cd33 binding polypeptides with stefin a protein
EP4412711A1 (en) 2021-10-07 2024-08-14 Avacta Life Sciences Limited Serum half-life extended pd-l1 binding polypeptides
US20250243277A1 (en) 2021-10-07 2025-07-31 Avacta Life Sciences Limited Pd-l1 binding affimers
US12234475B2 (en) * 2021-10-25 2025-02-25 Genvivo, Inc. Compositions and methods for therapeutic delivery
CN113999873B (en) * 2021-12-31 2022-05-20 北京市疾病预防控制中心 Construction method and application of genetically modified non-human animal
US20250163454A1 (en) * 2022-02-09 2025-05-22 The Regents Of The University Of California In vitro and in vivo protein translation via in situ circularized rnas
CN114478713B (en) * 2022-02-21 2023-03-28 江苏蒙彼利生物科技有限公司 CMV envelope protein packaging lentiviral vector and application thereof
TW202400647A (en) 2022-04-29 2024-01-01 美商普瑞諾生物科技公司 Methods and compositions for treating eosinophil driven diseases and disorders
US20260028602A1 (en) * 2022-08-09 2026-01-29 Asimov Inc. Split recombinases having inducible recombinase activity
CN116286993A (en) * 2023-03-01 2023-06-23 武汉科技大学 A kind of establishment method and application of HIV-tat-induced nerve injury model
WO2025029840A1 (en) 2023-07-31 2025-02-06 Tune Therapeutics, Inc. Compositions and methods for multiplexed activation and repression of t cell gene expression
WO2025038668A1 (en) 2023-08-14 2025-02-20 Voro Therapeutics, Inc. Therapeutic binding agents that conditionally promote myeloid cell activity against target cells and uses thereof
CN117264911A (en) * 2023-09-13 2023-12-22 中国科学院深圳先进技术研究院 Recombinant pseudorabies virus with high sensitivity for expressing red fluorescent protein, and preparation method and application thereof
WO2025239464A1 (en) * 2024-05-14 2025-11-20 대한뉴팜(주) Novel peptide and antiviral use thereof

Family Cites Families (62)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU680508B2 (en) 1993-04-06 1997-07-31 Government Of The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services, The Gibbon ape leukemia virus-based retroviral vectors
US20030157070A1 (en) 1994-12-30 2003-08-21 Jolly Douglas J. High efficiency ex vivo transduction of cells by high titer recombinant retroviral preparations
US6117681A (en) 1995-03-29 2000-09-12 Bavarian Nordic Research Inst. A/S Pseudotyped retroviral particles
US20030026789A1 (en) 1995-05-03 2003-02-06 Richard J. Gregory Gene therapy using replication competent targeted adenoviral vectors
US5912167A (en) * 1995-06-06 1999-06-15 Wisconsin Alumni Research Foundation Autocatalytic cleavage site and use thereof in a protein expression vector
WO1999020742A2 (en) 1997-10-20 1999-04-29 Universita' Degli Studi Di Padova A packaging cell line producing siv-pseudotyped mlv
FR2773561A1 (en) 1998-01-15 1999-07-16 Centre Nat Rech Scient USE OF A PROLINE RICH SEQUENCE TO INCREASE THE FUSOGENICITY OF RETROVIRUS ENVELOPES
US6451304B1 (en) 1998-03-09 2002-09-17 The Regents Of The University Of California Method for retrovirus vector production by separated gag and pol expression
WO1999055894A1 (en) 1998-04-29 1999-11-04 Oklahoma Medical Research Foundation Construction of retroviral producer cells from adenoviral and retroviral vectors
US7001733B1 (en) 1998-05-12 2006-02-21 Rigel Pharmaceuticals, Inc. Methods and compositions for screening for modulations of IgE synthesis, secretion and switch rearrangement
US6448390B1 (en) 1998-05-20 2002-09-10 The University Of Tennessee Research Corporation Stable envelope proteins for retroviral, viral and liposome vectors and use in gene drug therapy
US6264940B1 (en) 1998-08-05 2001-07-24 The Research Foundation Of State University Of New York Recombinant poliovirus for the treatment of cancer
EP2386646B1 (en) 1998-10-01 2016-12-21 University Of Southern California Gene delivery system and methods of use
US6899871B2 (en) 1998-10-01 2005-05-31 University Of Southern California Gene delivery system and methods of use
US6576463B1 (en) 1999-01-15 2003-06-10 The Regents Of The University Of California Hybrid vectors for gene therapy
JP2002542834A (en) 1999-04-29 2002-12-17 アールフス・ユニバーシティー Expression of heterologous genes from IRES transcription cassette in retroviral vector
GB2368846B (en) 1999-07-08 2004-08-25 Univ California Isolated Jaagsiekte retroviruses (JSRV), gene delivery vectors, and methods of use
AU7521500A (en) 1999-11-30 2001-06-12 Novartis Ag Increased transgene expression in retroviral vectors having scaffold attachment region
US6692736B2 (en) 2000-03-24 2004-02-17 Cell Genesys, Inc. Cell-specific adenovirus vectors comprising an internal ribosome entry site
AU2001292711A1 (en) 2000-09-18 2002-03-26 Genetic Therapy, Inc. Stress resistant retroviruses
US20030003565A1 (en) 2000-11-27 2003-01-02 Dubensky Thomas W. Functional lentiviral vector from an MLV-based backbone
US7226779B2 (en) 2001-01-30 2007-06-05 The United States Of America As Represented By The Department Of Health And Human Services Hybrid adenoviral vector
DE10111433A1 (en) 2001-03-09 2002-09-19 Bundesrepublik Deutschland Let Replication-competent molecular clones of porcine endogenous retrovirus class A and class B, derived from porcine and human cells
JP2002335965A (en) 2001-05-14 2002-11-26 Japan Science & Technology Corp Cell-specific expression replication vector
DE10143237A1 (en) 2001-09-04 2003-03-20 Icon Genetics Ag Manufacture of artificial internal ribosomal entry point elements (Ires elements)
US7732193B2 (en) 2001-09-13 2010-06-08 California Institute Of Technology Method for expression of small RNA molecules within a cell
FR2832424B1 (en) 2001-11-20 2004-09-24 Genethon Iii CHIMERIC PLASMID COMPRISING A REPLICATIVE RETROVIRAL GENOME AND USES
CA2474777A1 (en) 2002-02-01 2003-08-07 Transgene S.A. Adenoviral vectors for modulating the cellular activities associated with pods
EP1490023A4 (en) * 2002-02-28 2006-07-12 Iconix Pharm Inc SIGNATURES OF MEDICINAL PRODUCTS
JP2005532814A (en) 2002-07-17 2005-11-04 ブリストル−マイヤーズ　スクイブ　カンパニー Transgenic non-human mammal expressing reporter nucleic acid under the control of androgen response element
WO2005086922A2 (en) 2004-03-10 2005-09-22 Board Of Regents, University Of Texas System Oncolytic adenovirus armed with therapeutic genes
US20080227736A1 (en) 2004-06-03 2008-09-18 Regents Of The University Of California, Targeting Pseudotyped Retroviral Vectors
US20070003522A1 (en) 2004-07-08 2007-01-04 Albritton Lorraine M Methods and compositions for improved retroviral gene and drug delivery
CA2586844A1 (en) 2004-11-04 2006-05-11 Pfizer Products Inc. Ctla-4 antibody and aromatase inhibitor or combination treatment for breast cancer
JP2008521405A (en) 2004-11-24 2008-06-26 ナノヴェクター・リミテッド Virus vector
US7968698B2 (en) 2005-05-25 2011-06-28 The Regents Of The University Of California Optimized core promoters and uses thereof
EP2386564B1 (en) 2005-10-01 2014-07-16 Charles Stout Regulatable fusion promoters
WO2007044483A2 (en) 2005-10-07 2007-04-19 President And Fellows Of Harvard College Attenuated vaccines for non-segmented negative sense rna viruses
EP1795596A1 (en) 2005-12-08 2007-06-13 Ganymed Pharmaceuticals AG Composition and methods for therapy and diagnosis of cancer
WO2007095201A2 (en) 2006-02-15 2007-08-23 The Regents Of The University Of California Pseudotyped retroviral vectors and methods of use thereof
WO2007107156A2 (en) 2006-03-17 2007-09-27 Aarhus Universitet Chimeric viral envelopes
US20100178671A1 (en) * 2006-07-24 2010-07-15 Ab Enzymes Gmbh Vector constructs and methods for expressing and secreting polypeptides in filamentous fungi using a self-processing 2a cleavage site
WO2008151633A2 (en) 2007-06-15 2008-12-18 Skau Aps Vectors for vaccines against lentivirus infections
US20110136730A1 (en) * 2008-04-18 2011-06-09 John Fuller Atkins "Method for screening compounds comprising the use of picornavirus protease 2A"
EP2300611B1 (en) * 2008-06-13 2017-08-09 Whitehead Institute for Biomedical Research Programming and reprogramming of cells
MX337213B (en) 2008-06-30 2016-02-17 Tocagen Inc Formulations of 5-fluorocytosine and uses thereof.
JP5684130B2 (en) * 2008-09-26 2015-03-11 トカジェン インコーポレーテッド Recombinant vector
US8829173B2 (en) 2008-09-26 2014-09-09 Tocagen Inc. Recombinant vectors
CN101367749B (en) * 2008-09-28 2013-04-24 中国医学科学院医药生物技术研究所 A set of mesidino mesitoyl derivant, preparation and application thereof
CA2743669C (en) * 2008-11-24 2018-10-16 Helmholtz Zentrum Muenchen Deutsches Forschungszentrum Fuer Gesundheit Und Umwelt (Gmbh) High affinity t cell receptor and use thereof
CN101532030A (en) * 2009-04-21 2009-09-16 中国科学院广州生物医药与健康研究院 Recombinant influenza virus vector carrying foreign genes in NA segment and preparation method and application thereof
JP5923037B2 (en) * 2009-06-17 2016-05-24 トカジェン インコーポレーテッド Production cell of replicable retroviral vector
CN101709084B (en) * 2009-11-26 2012-07-04 中国科学院广州生物医药与健康研究院 Optimized serial cytokines and application thereof in inducing pluripotent stem cells
CA2805941A1 (en) 2010-08-13 2012-02-16 Pioneer Hi-Bred International, Inc. Methods and compositions for targeting sequences of interest to the chloroplast
EP3623478A1 (en) * 2012-10-25 2020-03-18 Tocagen Inc. Retroviral vector with mini-promoter cassette
US20150086509A1 (en) * 2013-09-25 2015-03-26 The Trustees Of Columbia University In The City Of New York Compositions and methods for treating cancer using interferon and mapk pathway inhibitor
GB201405845D0 (en) * 2014-04-01 2014-05-14 Ucl Business Plc Signalling system
GB201507104D0 (en) * 2015-04-27 2015-06-10 Ucl Business Plc Nucleic acid construct
CN104946686A (en) * 2015-05-29 2015-09-30 广东大华农动物保健品股份有限公司 Recombinant plasmid for expressing M protein, N protein and S1 protein in porcine epidemic diarrhea proteins, and construction method and application thereof
WO2017040815A1 (en) * 2015-09-04 2017-03-09 Tocagen Inc. Recombinant vectors comprising 2a peptide
GB201812474D0 (en) * 2018-07-31 2018-09-12 Autolus Ltd Nucleic acid construct
CN110564772B (en) * 2019-04-03 2022-07-05 安泰吉（北京）生物技术有限公司 Methods of engineering host cell genomes and uses thereof

Also Published As

Publication number Publication date
IL257819A (en) 2018-04-30
KR20180048743A (en) 2018-05-10
MX2018002744A (en) 2018-04-13
WO2017040815A1 (en) 2017-03-09
HK1251612A1 (en) 2019-02-01
EP3325633A4 (en) 2019-01-16
PH12018500477A1 (en) 2018-09-10
CA2996797A1 (en) 2017-03-09
US11279949B2 (en) 2022-03-22
JP2018526007A (en) 2018-09-13
AU2016317936A1 (en) 2018-03-08
CN108138201A (en) 2018-06-08
US20180251786A1 (en) 2018-09-06
EP3325633A1 (en) 2018-05-30

Similar Documents

Publication Publication Date Title
EA201890640A1 (en) RECOMBINANT VECTORS CONTAINING PEPTIDE 2A
EA201791426A1 (en) POLYPEPTIDE AND IMMUNOMODULATION
EA202091710A1 (en) ANTIBODIES AGAINST CD73 AND METHODS OF THEIR USE
PH12019500709A1 (en) Anti-lag-3 antibodies and methods of use thereof
EA201991383A1 (en) ANTIBODIES AGAINST CTLA-4 AND WAYS OF THEIR APPLICATION
EA201991720A1 (en) ANTIBODIES TO ALPHA-SINUCLEIN AND THEIR APPLICATIONS
EA201891137A1 (en) VIRAL VECTORS ENCODING FVIII RECOMBINANT OPTIONS WITH INCREASED EXPRESSION FOR HEMOPHILIA A GENE THERAPY
EA202091995A1 (en) COMPOSITIONS AND METHODS FOR TREATMENT OF AGE-NON-AGE-RELATED HEARING IN A HUMAN SUBJECT
MX2018009375A (en) OPTIMIZED FACTOR GENES VIII.
EA201792573A1 (en) TRISPECIFIC RELATED PROTEINS AND METHODS OF THEIR APPLICATION
EA201990912A1 (en) ANTI-LAG-3 ANTIBODIES AND THEIR COMPOSITIONS
EA201791775A1 (en) CYTESTINE PROTEASIS
EA201892225A1 (en) ANTIBODIES TO THE COMPLEX Bb FACTOR AND THEIR APPLICATION
EA201790413A1 (en) ANTIBODIES AGAINST TIGIT
EA201892561A1 (en) HYBRID PROTEINS GDF15 AND THEIR APPLICATION
EA201891732A1 (en) BISPECIFIC BINDING PROTEINS FOR PD-L1 AND KDR
EA201792651A1 (en) Adenoviral polynucleotides and polypeptides
CY1123031T1 (en) REGULATORS OF SUPPLEMENT EFFECTIVENESS
EA201691541A1 (en) NEW ANTI-BAFF ANTIBODIES
EA201890861A1 (en) COMPOSITIONS, CONTAINING LENTIVIRUS VECTORS, EXPRESSING IL-12, AND METHODS OF THEIR APPLICATION
MX382218B (en) LIPASE VARIANTS AND THE POLYNUCLEOTIDES THAT ENCODE THEM.
EA201890351A1 (en) RECOMBINANT ORF-VIRAL VECTOR
EA201992206A1 (en) MONOCLONAL ANTIBODY TO PD-L1
EA201892624A1 (en) PLANTS GIVING SEEDLESS FRUITS
EA201891138A1 (en) VIRAL VECTORS ENCODING FVIII RECOMBINANT OPTIONS WITH INCREASED EXPRESSION FOR HEMOPHILIA A GENE THERAPY