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EA201290684A1 - Способ получения биологически активного рекомбинантного г-ксф человека - Google Patents

Способ получения биологически активного рекомбинантного г-ксф человека

Info

Publication number
EA201290684A1
EA201290684A1 EA201290684A EA201290684A EA201290684A1 EA 201290684 A1 EA201290684 A1 EA 201290684A1 EA 201290684 A EA201290684 A EA 201290684A EA 201290684 A EA201290684 A EA 201290684A EA 201290684 A1 EA201290684 A1 EA 201290684A1
Authority
EA
Eurasian Patent Office
Prior art keywords
biologically active
active recombinant
csf
obtaining biologically
csf human
Prior art date
Application number
EA201290684A
Other languages
English (en)
Other versions
EA022821B1 (ru
Inventor
Вальтер Хиндерер
Кристиан Шекерманн
Original Assignee
Байодженерикс Гмбх
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Байодженерикс Гмбх filed Critical Байодженерикс Гмбх
Publication of EA201290684A1 publication Critical patent/EA201290684A1/ru
Publication of EA022821B1 publication Critical patent/EA022821B1/ru

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/53—Colony-stimulating factor [CSF]
    • C07K14/535—Granulocyte CSF; Granulocyte-macrophage CSF
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Toxicology (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Engineering & Computer Science (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicinal Preparation (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Предусмотрен способ получения биологически активного рекомбинантного Г-КСФ человека из телец включения, согласно которому процесс солюбилизации и рефолдинга можно осуществлять при температуре окружающей среды, а этап очистки включает хроматографию с обращенными фазами (ОФ), в частности ОФ-ВЭЖХ. Препарат Г-КСФ, полученный таким способом, отличается высокой чистотой и гомогенностью.
EA201290684A 2010-03-17 2011-03-17 Способ получения биологически активного рекомбинантного г-ксф человека EA022821B1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP10002811 2010-03-17
PCT/EP2011/001331 WO2011113601A1 (en) 2010-03-17 2011-03-17 Method for obtaining biologically active recombinant human g-csf

Publications (2)

Publication Number Publication Date
EA201290684A1 true EA201290684A1 (ru) 2013-03-29
EA022821B1 EA022821B1 (ru) 2016-03-31

Family

ID=42153771

Family Applications (1)

Application Number Title Priority Date Filing Date
EA201290684A EA022821B1 (ru) 2010-03-17 2011-03-17 Способ получения биологически активного рекомбинантного г-ксф человека

Country Status (16)

Country Link
US (1) US8703123B2 (ru)
EP (1) EP2547699B1 (ru)
JP (1) JP5964757B2 (ru)
KR (1) KR101853146B1 (ru)
CN (1) CN102892780B (ru)
AU (1) AU2011229491B2 (ru)
BR (1) BR112012022518A2 (ru)
CA (1) CA2789615A1 (ru)
EA (1) EA022821B1 (ru)
ES (1) ES2611453T3 (ru)
IL (1) IL221808B (ru)
MX (1) MX2012010661A (ru)
NZ (1) NZ601759A (ru)
PL (1) PL2547699T3 (ru)
WO (1) WO2011113601A1 (ru)
ZA (1) ZA201206208B (ru)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102695594A (zh) * 2010-03-05 2012-09-26 Dic株式会社 赋型片及其制造方法
HUP1200172A2 (en) * 2012-03-19 2013-10-28 Richter Gedeon Nyrt Methods for refolding g-csf from inclusion bodies
CN103908427B (zh) * 2013-01-05 2014-12-17 石药集团百克(山东)生物制药有限公司 一种聚乙二醇修饰的rhG-CSF注射液及其制备方法
EP2978770B1 (en) 2013-03-29 2019-11-06 Dr. Reddy's Laboratories Limited Refolding of proteins
WO2014155365A2 (en) * 2013-03-29 2014-10-02 Dr.Reddy's Laboratories Limited Purification method
CN103694339B (zh) * 2013-12-04 2017-10-13 珠海联邦制药股份有限公司 一种甘精胰岛素前体的复性方法
US10457716B2 (en) 2014-08-06 2019-10-29 University Of Notre Dame Du Lac Protein folding and methods of using same
BR112017013398B1 (pt) 2014-12-22 2023-12-05 Ucb Biopharma Sprl Método de fabricar anticorpo ou fragmento de ligação ao antígeno do mesmo
JP2016183118A (ja) * 2015-03-25 2016-10-20 東ソー株式会社 増殖因子前駆体のリフォールディング方法
GB201506870D0 (en) * 2015-04-22 2015-06-03 Ucb Biopharma Sprl Method
WO2018132787A1 (en) 2017-01-16 2018-07-19 Nansha Biologics (Hk) Limited Systems and methods for production of recombinant il-11 in yeast
CN118063587A (zh) * 2024-04-17 2024-05-24 山东泉港药业有限公司 一种降低重组人粒细胞刺激因子原液相关蛋白含量的工艺方法

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GR79124B (ru) 1982-12-22 1984-10-02 Genentech Inc
DE122080T1 (de) 1983-03-25 1985-05-23 Celltech Ltd., Slough, Berkshire Verfahren zur herstellung eines proteins.
US4530787A (en) 1984-03-28 1985-07-23 Cetus Corporation Controlled oxidation of microbially produced cysteine-containing proteins
DE3537708A1 (de) 1985-10-23 1987-04-23 Boehringer Mannheim Gmbh Verfahren zur aktivierung von t-pa nach expression in prokaryonten
DE3611817A1 (de) 1986-04-08 1987-10-15 Boehringer Mannheim Gmbh Verfahren zur renaturierung von proteinen
NZ229098A (en) 1988-05-13 1992-10-28 Amgen Inc (bg-csf) bovine granulocyte colony stimulating factor, recombinant methods and pharmaceuticals
DE3835350A1 (de) 1988-10-17 1990-04-19 Boehringer Mannheim Gmbh Aktivierung von gentechnologisch hergestellten, in prokaryonten exprimierten antikoerpern
JPH02234679A (ja) * 1989-03-07 1990-09-17 Sagami Chem Res Center ヒトラミニンｂ１鎖ポリペプチド断片及びこれを生産するための発現ベクター
FR2669646A1 (fr) 1990-11-23 1992-05-29 Lorraine Laminage Procede de traitement d'un effluent aqueux issu d'un procede d'electrozingage, ainsi que procede et installation en comportant application.
DE4105480A1 (de) 1991-02-21 1992-08-27 Boehringer Mannheim Gmbh Verbesserte aktivierung von rekombinanten proteinen
ES2290142T3 (es) 2000-05-16 2008-02-16 Bolder Biotechnology, Inc. Metodos para replegamiento de proteinas que contiene residuos de cisteina libre.
DE102004041639A1 (de) * 2004-08-27 2006-03-02 Bioceuticals Arzneimittel Ag Verfahren zur Gewinnung von biologisch aktivem humanen G-CSF aus Inclusion Bodies
DE102005033250A1 (de) * 2005-07-15 2007-01-18 Bioceuticals Arzneimittel Ag Verfahren zur Reinigung von G-CSF
GB0605684D0 (en) * 2006-03-21 2006-05-03 Sicor Biotech Uab Method For Purifying Granulocyte-Colony Stimulating Factor
WO2008096370A2 (en) * 2007-02-05 2008-08-14 Natco Pharma Limited An efficient and novel purification method of recombinant hg-csf
CA2682897C (en) 2007-04-03 2016-11-22 Biogenerix Ag Methods of treatment using glycopegylated g-csf
UA98001C2 (ru) 2007-08-27 2012-04-10 Биодженерикс Аг Жидкая лекарственная форма конъюгата полимер-g-csf
EP2330128B1 (en) * 2008-08-27 2015-12-23 LSI Medience Corporation Modified anti-heparin/pf4 complex antibody and hit antibody standard

Also Published As

Publication number Publication date
CN102892780A (zh) 2013-01-23
CA2789615A1 (en) 2011-09-22
IL221808B (en) 2018-06-28
KR101853146B1 (ko) 2018-04-27
AU2011229491A1 (en) 2012-09-06
ES2611453T3 (es) 2017-05-09
CN102892780B (zh) 2015-07-29
NZ601759A (en) 2013-07-26
EP2547699A1 (en) 2013-01-23
AU2011229491B2 (en) 2015-02-05
KR20130054948A (ko) 2013-05-27
WO2011113601A1 (en) 2011-09-22
MX2012010661A (es) 2013-03-25
US8703123B2 (en) 2014-04-22
US20120328560A1 (en) 2012-12-27
JP5964757B2 (ja) 2016-08-03
EP2547699B1 (en) 2016-10-19
JP2013522252A (ja) 2013-06-13
EA022821B1 (ru) 2016-03-31
PL2547699T3 (pl) 2017-04-28
BR112012022518A2 (pt) 2020-10-13
ZA201206208B (en) 2013-05-29

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Legal Events

Date Code Title Description
MM4A Lapse of a eurasian patent due to non-payment of renewal fees within the time limit in the following designated state(s)

Designated state(s): AM AZ BY KZ KG MD TJ TM RU