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EA201070259A1 - Термофильные микроорганизмы для продуцирования этанола - Google Patents

Термофильные микроорганизмы для продуцирования этанола

Info

Publication number
EA201070259A1
EA201070259A1 EA201070259A EA201070259A EA201070259A1 EA 201070259 A1 EA201070259 A1 EA 201070259A1 EA 201070259 A EA201070259 A EA 201070259A EA 201070259 A EA201070259 A EA 201070259A EA 201070259 A1 EA201070259 A1 EA 201070259A1
Authority
EA
Eurasian Patent Office
Prior art keywords
ethanol
production
thermophilic microorganisms
modification
amylase
Prior art date
Application number
EA201070259A
Other languages
English (en)
Other versions
EA018814B1 (ru
Inventor
Энтони Аткинсон
Роджер Криппс
Кирстин Элей
Брайан Рудд
Мартин Тодд
Original Assignee
Тмо Реньюаблз Лимитед
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Тмо Реньюаблз Лимитед filed Critical Тмо Реньюаблз Лимитед
Publication of EA201070259A1 publication Critical patent/EA201070259A1/ru
Publication of EA018814B1 publication Critical patent/EA018814B1/ru

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/065—Ethanol, i.e. non-beverage with microorganisms other than yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Fodder In General (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Термофильный микроорганизм, содержащий модификацию, повышающую экспрессию амилазы и гидролиз крахмала по сравнению с диким типом, в котором модификация представляет собой инсерцию гетерологичного гена амилазы.
EA201070259A 2007-08-13 2008-08-12 Термофильный микроорганизм для повышенной продукции этанола из крахмалсодержащего сырья и способ обеспечения повышенной продукции этанола при его использовании EA018814B1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB0715751.4A GB0715751D0 (en) 2007-08-13 2007-08-13 Thermophilic micro-organisms for ethanol production
PCT/GB2008/002782 WO2009022158A1 (en) 2007-08-13 2008-08-12 Thermophilic micro-organisms for ethanol production

Publications (2)

Publication Number Publication Date
EA201070259A1 true EA201070259A1 (ru) 2010-10-29
EA018814B1 EA018814B1 (ru) 2013-10-30

Family

ID=38543482

Family Applications (1)

Application Number Title Priority Date Filing Date
EA201070259A EA018814B1 (ru) 2007-08-13 2008-08-12 Термофильный микроорганизм для повышенной продукции этанола из крахмалсодержащего сырья и способ обеспечения повышенной продукции этанола при его использовании

Country Status (14)

Country Link
US (3) US8021865B2 (ru)
EP (2) EP2568039A1 (ru)
JP (1) JP2010536334A (ru)
KR (1) KR20100061460A (ru)
CN (1) CN101802178A (ru)
AU (1) AU2008288285B2 (ru)
BR (1) BRPI0814261A2 (ru)
CA (1) CA2696277A1 (ru)
EA (1) EA018814B1 (ru)
GB (2) GB0715751D0 (ru)
MX (1) MX2010001655A (ru)
NZ (1) NZ583216A (ru)
WO (1) WO2009022158A1 (ru)
ZA (1) ZA201000969B (ru)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BRPI0610988A2 (pt) * 2005-05-04 2010-08-10 Tmo Renewables Ltd microorganismos termofìlicos com gene de lactato desidrogenase inativado (ldh) para produção de etanol
GB0511602D0 (en) * 2005-06-07 2005-07-13 Tmo Biotec Ltd Microorganisms
CA2660486A1 (en) * 2006-09-28 2008-04-03 Tmo Renewables Limited Thermophilic microorganisms for ethanol production
GB0715751D0 (en) * 2007-08-13 2007-09-19 Tmo Renewables Ltd Thermophilic micro-organisms for ethanol production
GB0820262D0 (en) 2008-11-05 2008-12-10 Tmo Renewables Ltd Microorganisms
WO2011092638A2 (en) 2010-01-26 2011-08-04 Scale Biofuel Aps Methods for producing and harvesting ethanol and apparatus for producing and harvesting the same
EP2710308A1 (en) 2011-05-18 2014-03-26 Scale Biofuel APS Solar-assisted volatile fermentation products production processes
US9631206B2 (en) * 2012-08-30 2017-04-25 Department Of Biotechnology Ministry Of Science & Technology Modified bacteria for the production of bioalcohol
CN111154705B (zh) * 2020-01-07 2021-06-29 中国科学院微生物研究所 热葡萄糖苷酶地芽孢杆菌工程菌及其构建方法及应用
WO2022047264A1 (en) * 2020-08-28 2022-03-03 Codexis, Inc. Engineered amylase variants

Family Cites Families (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1174191A (en) 1980-03-05 1984-09-11 Peter L. Rogers Ethanol production
JPS59196092A (ja) * 1983-04-25 1984-11-07 Sanraku Inc 組換えプラスミドによる耐熱性α−アミラ−ゼの新規製造法
US5238833A (en) * 1983-07-06 1993-08-24 Gist-Brocades, Nv Molecular cloning and expression in industrial Bacillus species
EP0134048B2 (en) * 1983-07-06 1996-08-14 Gist-Brocades N.V. Molecular cloning and expression in industrial microorganism species
JPS61166400A (ja) * 1985-01-18 1986-07-28 Agency Of Ind Science & Technol 蛋白生産の方法
GB2171703B (en) * 1985-01-18 1989-11-15 Agency Ind Science Techn Expression-secretion vector for gene expression and protein secretion, recombinant dna including the vector, and method of producing proteins by use of the re
DK122686D0 (da) * 1986-03-17 1986-03-17 Novo Industri As Fremstilling af proteiner
GB8712410D0 (en) 1987-05-27 1987-07-01 Elsworth Biotech Ltd Thermophilic ethanol production
DE3824827A1 (de) * 1988-07-21 1990-02-01 Henkel Kgaa Plasmide zur erzeugung von alpha-amylase in bacillus
US5482846A (en) 1988-08-31 1996-01-09 University Of Florida Ethanol production in Gram-positive microbes
JP2995770B2 (ja) * 1989-12-15 1999-12-27 吉富製薬株式会社 異種蛋白質の製造方法
EP0626012B1 (en) 1992-02-11 2003-07-09 Cell Genesys, Inc. Homogenotization of gene-targeting events
US5602004A (en) * 1994-07-20 1997-02-11 Novo Nordisk Biotech, Inc. Thermophilic fungal expression system
AU6875298A (en) 1997-04-07 1998-10-30 University Of Florida Research Foundation, Inc. Development of high-ethanol resistant (escherichia coli)
EP0937774A1 (en) 1998-02-20 1999-08-25 Chr. Hansen A/S Lactic acid bacteria having a reduced pyruvate dehydrogenase activity and use thereof
GB0000185D0 (en) 2000-01-06 2000-03-01 Agrol Limited Ethanol production
US7374939B1 (en) 2000-05-01 2008-05-20 Midwest Research Institute Method of inactivation of an end product of energy metabolism in Zymomonas mobilis
GB0011186D0 (en) 2000-05-09 2000-06-28 Agrol Limited Modification of bacteria
WO2002029030A2 (en) 2000-10-06 2002-04-11 Elsworth Biotechnology Limited Ethanol production
JP4192239B2 (ja) * 2003-11-27 2008-12-10 独立行政法人産業技術総合研究所 出芽酵母アピラーゼの結晶および該結晶の製造方法
JP2005261239A (ja) 2004-03-17 2005-09-29 Japan Steel Works Ltd:The 低級アルコールの製造法
BRPI0610988A2 (pt) * 2005-05-04 2010-08-10 Tmo Renewables Ltd microorganismos termofìlicos com gene de lactato desidrogenase inativado (ldh) para produção de etanol
GB0511602D0 (en) 2005-06-07 2005-07-13 Tmo Biotec Ltd Microorganisms
GB0520344D0 (en) 2005-10-06 2005-11-16 Tmo Biotec Ltd Microoganisms
MX2008014733A (es) * 2006-05-22 2009-02-12 Biogasol Ipr Aps Cepa bg1 de thermoanaerobacter mathranii.
CA2660486A1 (en) 2006-09-28 2008-04-03 Tmo Renewables Limited Thermophilic microorganisms for ethanol production
GB0715751D0 (en) * 2007-08-13 2007-09-19 Tmo Renewables Ltd Thermophilic micro-organisms for ethanol production
GB0820262D0 (en) 2008-11-05 2008-12-10 Tmo Renewables Ltd Microorganisms

Also Published As

Publication number Publication date
US20110201074A1 (en) 2011-08-18
GB2464248B (en) 2011-03-02
AU2008288285B2 (en) 2011-11-24
CN101802178A (zh) 2010-08-11
US8021865B2 (en) 2011-09-20
US8143038B2 (en) 2012-03-27
WO2009022158A8 (en) 2009-07-16
NZ583216A (en) 2011-12-22
BRPI0814261A2 (pt) 2014-10-21
US8852906B2 (en) 2014-10-07
WO2009022158A1 (en) 2009-02-19
US20130059355A1 (en) 2013-03-07
JP2010536334A (ja) 2010-12-02
ZA201000969B (en) 2010-10-27
EP2185697A1 (en) 2010-05-19
US20110318802A1 (en) 2011-12-29
MX2010001655A (es) 2010-07-28
AU2008288285A1 (en) 2009-02-19
CA2696277A1 (en) 2009-02-19
KR20100061460A (ko) 2010-06-07
GB201002452D0 (en) 2010-03-31
GB2464248A (en) 2010-04-14
EA018814B1 (ru) 2013-10-30
EP2568039A1 (en) 2013-03-13
GB0715751D0 (en) 2007-09-19

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Legal Events

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MM4A Lapse of a eurasian patent due to non-payment of renewal fees within the time limit in the following designated state(s)

Designated state(s): AM AZ BY KZ KG MD TJ TM RU