EA200000029A1 - EXPRESSION OF FRUCTOSE-1,6-BISPHOSPHATALATOLDOLASE In TRANSGENIC PLANTS - Google Patents
EXPRESSION OF FRUCTOSE-1,6-BISPHOSPHATALATOLDOLASE In TRANSGENIC PLANTSInfo
- Publication number
- EA200000029A1 EA200000029A1 EA200000029A EA200000029A EA200000029A1 EA 200000029 A1 EA200000029 A1 EA 200000029A1 EA 200000029 A EA200000029 A EA 200000029A EA 200000029 A EA200000029 A EA 200000029A EA 200000029 A1 EA200000029 A1 EA 200000029A1
- Authority
- EA
- Eurasian Patent Office
- Prior art keywords
- leaves
- fda
- plants
- fructose
- sucrose
- Prior art date
Links
- 230000009261 transgenic effect Effects 0.000 title abstract 4
- 241000196324 Embryophyta Species 0.000 abstract 5
- 229920002472 Starch Polymers 0.000 abstract 3
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 abstract 3
- 229930006000 Sucrose Natural products 0.000 abstract 3
- 230000015572 biosynthetic process Effects 0.000 abstract 3
- 239000008107 starch Substances 0.000 abstract 3
- 235000019698 starch Nutrition 0.000 abstract 3
- 239000005720 sucrose Substances 0.000 abstract 3
- 108090000790 Enzymes Proteins 0.000 abstract 2
- 102000004190 Enzymes Human genes 0.000 abstract 2
- 102000001390 Fructose-Bisphosphate Aldolase Human genes 0.000 abstract 2
- 108010068561 Fructose-Bisphosphate Aldolase Proteins 0.000 abstract 2
- 210000003763 chloroplast Anatomy 0.000 abstract 2
- XPYBSIWDXQFNMH-UHFFFAOYSA-N D-fructose 1,6-bisphosphate Natural products OP(=O)(O)OCC(O)C(O)C(O)C(=O)COP(O)(O)=O XPYBSIWDXQFNMH-UHFFFAOYSA-N 0.000 abstract 1
- 241000588724 Escherichia coli Species 0.000 abstract 1
- 235000002595 Solanum tuberosum Nutrition 0.000 abstract 1
- 244000061456 Solanum tuberosum Species 0.000 abstract 1
- 108700019146 Transgenes Proteins 0.000 abstract 1
- 238000009825 accumulation Methods 0.000 abstract 1
- RNBGYGVWRKECFJ-ZXXMMSQZSA-N alpha-D-fructofuranose 1,6-bisphosphate Chemical compound O[C@H]1[C@H](O)[C@](O)(COP(O)(O)=O)O[C@@H]1COP(O)(O)=O RNBGYGVWRKECFJ-ZXXMMSQZSA-N 0.000 abstract 1
- 238000006243 chemical reaction Methods 0.000 abstract 1
- 210000000172 cytosol Anatomy 0.000 abstract 1
- RNBGYGVWRKECFJ-UHFFFAOYSA-N fructose-1,6-phosphate Natural products OC1C(O)C(O)(COP(O)(O)=O)OC1COP(O)(O)=O RNBGYGVWRKECFJ-UHFFFAOYSA-N 0.000 abstract 1
- 235000012015 potatoes Nutrition 0.000 abstract 1
- 239000007787 solid Substances 0.000 abstract 1
- 238000003786 synthesis reaction Methods 0.000 abstract 1
- 238000009827 uniform distribution Methods 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/88—Lyases (4.)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
- C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Cell Biology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Nutrition Science (AREA)
- Medicinal Chemistry (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Preparation Of Fruits And Vegetables (AREA)
Abstract
Фруктозо-1,6-бисфосфатальдолаза (FDA) является ферментом, обратимо катализирующим реакцию превращения триозофосфата в фруктозо-1,6-бисфосфат. В листьях этот фермент расположен в хлоропластах (синтез крахмала) и в цитозоле (биосинтез сахарозы). Созданы трансгенные растения, которые экспрессируют fda E. coli в хлоропластах для повышения выхода растений за счет повышения способности листьев к биосинтезу крахмала в частности, и продуцированию сахарозы в целом. Листья растений, экспрессирующих трансген fda, демонстрируют значительно более высокое накопление крахмала по сравнению с контрольными растениями, экспрессирующими нулевой вектор, особенно в начале фотопериода, но содержат в листьях меньше сахарозы. Трансгенные растения обладают также значительно большей корневой массой. Кроме того, трансгенный картофель, экспрессирующий fda, демонстрирует повышенную равномерность распределения твердых веществ.Международная заявка была опубликована вместе с отчетом о международном поиске.Fructose-1,6-bisphosphate-aldolase (FDA) is an enzyme that reversibly catalyzes the conversion of triosophosphate to fructose-1,6-bisphosphate. In leaves, this enzyme is located in chloroplasts (starch synthesis) and in the cytosol (sucrose biosynthesis). Transgenic plants that express E. coli fda in chloroplasts are created to increase the yield of plants by increasing the ability of the leaves to starch biosynthesis in particular, and to produce sucrose as a whole. Leaves of plants expressing the fda transgene show a significantly higher starch accumulation compared to control plants expressing the zero vector, especially at the beginning of the photoperiod, but contain less sucrose in the leaves. Transgenic plants also have a significantly greater root mass. In addition, transgenic potatoes expressing fda show an increased uniform distribution of solids. The international application was published along with an international search report.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US4995597P | 1997-06-17 | 1997-06-17 | |
| PCT/US1998/012447 WO1998058069A1 (en) | 1997-06-17 | 1998-06-16 | Expression of fructose 1,6 bisphosphate aldolase in transgenic plants |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| EA200000029A1 true EA200000029A1 (en) | 2000-08-28 |
Family
ID=21962657
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| EA200000029A EA200000029A1 (en) | 1997-06-17 | 1998-06-16 | EXPRESSION OF FRUCTOSE-1,6-BISPHOSPHATALATOLDOLASE In TRANSGENIC PLANTS |
Country Status (16)
| Country | Link |
|---|---|
| EP (1) | EP1002114A1 (en) |
| JP (1) | JP2001520522A (en) |
| CN (1) | CN1267333A (en) |
| AR (1) | AR020307A1 (en) |
| AU (1) | AU735383B2 (en) |
| BR (1) | BR9810171A (en) |
| CA (1) | CA2294525A1 (en) |
| EA (1) | EA200000029A1 (en) |
| HU (1) | HUP0004228A3 (en) |
| IL (1) | IL133532A0 (en) |
| NO (1) | NO996218L (en) |
| NZ (1) | NZ501781A (en) |
| PL (1) | PL337581A1 (en) |
| TR (1) | TR199903157T2 (en) |
| WO (1) | WO1998058069A1 (en) |
| ZA (1) | ZA985261B (en) |
Families Citing this family (28)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2333148A1 (en) | 1998-07-10 | 2000-01-20 | Calgene Llc | Expression of eukaryotic peptides in plant plastids |
| US6512162B2 (en) | 1998-07-10 | 2003-01-28 | Calgene Llc | Expression of eukaryotic peptides in plant plastids |
| US7858848B2 (en) | 1999-11-17 | 2010-12-28 | Mendel Biotechnology Inc. | Transcription factors for increasing yield |
| US7868229B2 (en) | 1999-03-23 | 2011-01-11 | Mendel Biotechnology, Inc. | Early flowering in genetically modified plants |
| US7345217B2 (en) | 1998-09-22 | 2008-03-18 | Mendel Biotechnology, Inc. | Polynucleotides and polypeptides in plants |
| JP3357909B2 (en) | 1999-03-10 | 2002-12-16 | 奈良先端科学技術大学院大学長 | How to increase productivity of higher plants |
| ATE398182T1 (en) * | 1999-11-17 | 2008-07-15 | Mendel Biotechnology Inc | TOLERANCE GENES FOR ENVIRONMENTAL STRESS |
| CN101311267B (en) | 1999-12-16 | 2016-08-03 | 孟山都技术有限公司 | Novel plant expression constructs |
| AU2002230899B2 (en) * | 2000-10-30 | 2006-11-09 | Monsanto Technology Llc | Canola event PV-BNGT04(RT73) and compositions and methods for detection thereof |
| US7939715B2 (en) | 2000-11-16 | 2011-05-10 | Mendel Biotechnology, Inc. | Plants with improved yield and stress tolerance |
| US8426678B2 (en) | 2002-09-18 | 2013-04-23 | Mendel Biotechnology, Inc. | Polynucleotides and polypeptides in plants |
| JP2005130770A (en) | 2003-10-30 | 2005-05-26 | Ajinomoto Co Inc | Potato increased in starch level obtained per plant body and method for creating the same |
| CN104178511A (en) * | 2004-07-31 | 2014-12-03 | 梅坦诺米克斯有限公司 | Preparation of organisms with faster growth and/or higher yield |
| US20090081300A1 (en) * | 2005-11-16 | 2009-03-26 | Pro Natura Gesellschaft Fuer Gesunde Ernaehrung Mb | Agent for use in the case of fructose intolerance |
| BRPI0718438A2 (en) * | 2006-02-09 | 2013-01-01 | Japan Science & Tech Agency | plant having improved growth capacity and disease resistance and method for its production |
| CN102268430B (en) * | 2010-06-02 | 2013-05-15 | 中国科学院大连化学物理研究所 | Fructose-1,6-bisphosphate aldolase promoter and its application and construct, vector |
| US11913166B2 (en) | 2015-09-21 | 2024-02-27 | Modern Meadow, Inc. | Fiber reinforced tissue composites |
| KR20170096096A (en) | 2016-02-15 | 2017-08-23 | 브렌던 패트릭 퍼셀 | Method for making a biofabricated material containing collagen fibrils |
| CN105973851B (en) * | 2016-06-12 | 2018-10-23 | 中国科学院地球化学研究所 | A kind of bathmic method of quantitative determination plant photosynthesis |
| CA3008850A1 (en) | 2017-06-29 | 2018-12-29 | Modern Meadow, Inc. | Yeast strains and methods for producing collagen |
| AU2018253595A1 (en) | 2017-11-13 | 2019-05-30 | Modern Meadow, Inc. | Biofabricated leather articles having zonal properties |
| WO2020150443A1 (en) | 2019-01-17 | 2020-07-23 | Modern Meadow, Inc. | Layered collagen materials and methods of making the same |
| CN112094851B (en) * | 2020-09-25 | 2022-01-25 | 扬州大学 | Paeonia ostii PoCAB151 gene, expression vector, and preparation method and application thereof |
| CN112094859B (en) * | 2020-09-25 | 2022-01-25 | 扬州大学 | Paeonia ostii PoFBA gene, expression vector, preparation method and application thereof |
| CN113528567B (en) * | 2021-07-22 | 2022-05-03 | 中国林业科学研究院林业研究所 | Use of FBA8 protein or protein derived therefrom for regulating vascular bundle division and/or rachis cross-sectional area in plants |
| CN113528497B (en) * | 2021-07-22 | 2022-06-24 | 中国林业科学研究院林业研究所 | Use of FBA8 gene or FBA8 protein for preparing reagent with transphosphorylation activity and/or proteolytic activity |
| CN114920938B (en) * | 2022-05-29 | 2023-11-10 | 深圳绿天琪生物医药有限公司 | Preparation and application of arginine coupled plant source glycan and polypeptide synthetic copolymer |
| CN118086362B (en) * | 2024-01-31 | 2024-08-30 | 禾生创源(苏州)生物技术有限公司 | Application of rice gene OsALDO in regulation of rice grain type and mutant thereof |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ATE212670T1 (en) * | 1990-06-18 | 2002-02-15 | Monsanto Technology Llc | INCREASED STARCH CONTENT IN PLANTS |
| DE19502053A1 (en) * | 1995-01-13 | 1996-07-18 | Inst Genbiologische Forschung | Processes and DNA molecules to increase the rate of photosynthesis in plants, as well as plant cells and plants with an increased rate of photosynthesis |
| US5716837A (en) * | 1995-02-10 | 1998-02-10 | Monsanto Company | Expression of sucrose phosphorylase in plants |
-
1998
- 1998-06-16 EA EA200000029A patent/EA200000029A1/en unknown
- 1998-06-16 WO PCT/US1998/012447 patent/WO1998058069A1/en not_active Ceased
- 1998-06-16 AU AU81445/98A patent/AU735383B2/en not_active Ceased
- 1998-06-16 PL PL98337581A patent/PL337581A1/en unknown
- 1998-06-16 CN CN98808178A patent/CN1267333A/en active Pending
- 1998-06-16 HU HU0004228A patent/HUP0004228A3/en unknown
- 1998-06-16 EP EP98931283A patent/EP1002114A1/en not_active Withdrawn
- 1998-06-16 TR TR1999/03157T patent/TR199903157T2/en unknown
- 1998-06-16 BR BR9810171-4A patent/BR9810171A/en not_active Application Discontinuation
- 1998-06-16 IL IL13353298A patent/IL133532A0/en unknown
- 1998-06-16 NZ NZ501781A patent/NZ501781A/en unknown
- 1998-06-16 JP JP50465099A patent/JP2001520522A/en active Pending
- 1998-06-16 CA CA002294525A patent/CA2294525A1/en not_active Abandoned
- 1998-06-17 AR ARP980102895A patent/AR020307A1/en not_active Application Discontinuation
- 1998-06-17 ZA ZA985261A patent/ZA985261B/en unknown
-
1999
- 1999-12-15 NO NO996218A patent/NO996218L/en not_active Application Discontinuation
Also Published As
| Publication number | Publication date |
|---|---|
| ZA985261B (en) | 1999-01-07 |
| EP1002114A1 (en) | 2000-05-24 |
| IL133532A0 (en) | 2001-04-30 |
| NO996218D0 (en) | 1999-12-15 |
| AU735383B2 (en) | 2001-07-05 |
| NZ501781A (en) | 2001-07-27 |
| HUP0004228A3 (en) | 2002-11-28 |
| BR9810171A (en) | 2001-11-06 |
| AU8144598A (en) | 1999-01-04 |
| AR020307A1 (en) | 2002-05-08 |
| JP2001520522A (en) | 2001-10-30 |
| CA2294525A1 (en) | 1998-12-23 |
| CN1267333A (en) | 2000-09-20 |
| TR199903157T2 (en) | 2000-06-21 |
| HUP0004228A2 (en) | 2001-03-28 |
| NO996218L (en) | 2000-02-14 |
| PL337581A1 (en) | 2000-08-28 |
| WO1998058069A1 (en) | 1998-12-23 |
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