EA047911B1 - INJECTION SYSTEM FOR PLANTS - Google Patents

Description

Cross reference to related application

The invention claims priority to U.S. Provisional Patent Application No. 63/143,643, filed January 29, 2021, which is incorporated herein by reference in its entirety.

Field of technology

The present invention generally relates to systems and methods for administering formulations to plants, including injection instruments, systems incorporating such injection instruments, and methods of using such injection instruments and systems to administer liquid formulations to plants.

Background of the invention

Plant injection is used to introduce active ingredients into plants. Traditional approaches to plant injection may involve drilling a hole into a tree trunk and plugging the hole with a bung. A needle is inserted through the bung to release liquid into the hole.

What is desired in the art is an injection system that allows the user to easily control the depth of penetration or injection using an injection tool located or integrated into the injection system. What is also desired in the art is an injection system that can be conveniently used to inject liquid formulations into relatively small plants.

The essence of the invention

In some aspects, various chassis suitable for integrating plant injection system components, such as injection tools, are provided herein. Such chassis are intended for use with various types of plants, including plants with thinner stems and/or less mature stems. In some embodiments, plant injection systems comprising a housing or chassis described herein may be suitable for use in small-scale plant injections, such as in nurseries. In some embodiments, plant injection systems comprising a housing or chassis described herein prevent leakage and damage to plants, especially for plants with thinner and/or less mature stems.

In other aspects, the present document also provides plant injection systems comprising such a chassis for introducing fluids, such as liquid formulations comprising one or more active ingredients, into a plant or a part of a plant. In some embodiments, the plant injection systems further comprise an injection tool. In some embodiments, the chassis houses the injection tool. In some embodiments, the chassis is connected to a fluid delivery system that comprises a source of a liquid formulation. In some embodiments, the plant injection systems are configured to deliver the liquid formulation from the fluid delivery system through the injection tool into a plant (e.g., a stem or thin trunk of a plant).

In certain aspects, various clamp-style chassis and pushpin-style chassis are provided that are configured to integrate an injection tool and components for fluidly connecting the injection tool to a fluid delivery system; and to install the injection tool and maintain it in place after installation on a plant.

In one aspect described in this document, a clamp-style chassis comprises a large lever, a small lever and a pressing element. In some embodiments, the large lever comprises a first gripping end, a first driving end and a first connecting portion between the first gripping end and the first driving end. In some embodiments, the small lever comprises a second gripping end, a second driving end and a second connecting portion between the second gripping end and the second driving end. In some embodiments, the pressing element connects the first driving end and the second driving end and is configured to push the first gripping end and the second gripping end toward each other. In some embodiments, the first connecting portion is larger than the second connecting portion. In some embodiments, the first connecting portion is connected to the second connecting portion to form a hinge. In some embodiments, the first gripping end, the second gripping end and the first connecting portion form a grip profile.

In another aspect, the document describes methods of using an injection system for plants. In some embodiments, the method includes squeezing the first driving end and the second driving end toward each other. In some embodiments, the method includes placing a plant part between the first gripping lever and the second gripping lever. In some embodiments, the method includes introducing at least a portion of the injection tool into the plant part by pushing the chassis toward the plant part in the same direction as the longitudinal axis of the chassis. In some embodiments, the method includes releasing the first driving end and the second driving end. In some embodiments, the method includes injecting a liquid composition into the plant part.

In another aspect, the document describes a clamp-style chassis comprising a first arm, a second arm, a connecting portion, and a pressing member. In some embodiments,

- 1 047911 the first lever comprises a first gripping end and a first moving end. In some embodiments, the second lever comprises a second gripping end and a second moving end. In some embodiments, a connecting portion connects the first lever and the second lever between the gripping and moving ends.

In some embodiments, the pressing element connects the first moving end and the second moving end and is configured to push the first gripping end and the second gripping end toward each other. In some embodiments, the first gripping end, the second gripping end and the connecting section form a gripping profile.

In another aspect, the document describes methods of using an injection system for plants. In some embodiments, the method includes squeezing the first driving end and the second driving end toward each other. In some embodiments, the method includes placing a plant part between the first gripping lever and the second gripping lever. In some embodiments, the method includes introducing at least a portion of the injection tool into the plant part by pushing the chassis toward the plant part in the same direction as the longitudinal axis of the chassis. In some embodiments, the method includes releasing the first driving end and the second driving end. In some embodiments, the method includes injecting a liquid composition into the plant part.

In another aspect, another clamp-style chassis is described in this document, comprising a first lever, a second lever, a clamp and a pressing element. In some embodiments, the first lever comprises a first pivot end and a first moving end. In some embodiments, the second lever comprises a second pivot end and a second moving end. In some embodiments, the clamp comprises a first side, a second side and a base that form a U-shape having an inner surface and an outer surface. In some embodiments, the first and second pivot ends are mounted on the outer surface of the clamp on the base. In some embodiments, the pressing element is connected to the outer surface of the clamp on the first side and the second side. In some embodiments, the pressing element is configured to push the first side and the second side toward each other.

In another aspect, the document describes methods for using an injection system for plants. In some embodiments, the method includes placing a plant part between a first side and a second side. In some embodiments, the method includes introducing at least a portion of an injection tool into the plant part by pushing a chassis toward the plant part in the same direction as the longitudinal axis of the chassis. In some embodiments, the method includes introducing a fastener into one opening on the first side and a corresponding opening on the second side to clamp the plant part. In some embodiments, the method includes injecting a liquid composition into the plant part.

In one aspect, the document describes a pushpin-style chassis comprising a base, a pushpin, and one or more fastening elements. In some embodiments, the base has an inner surface and an outer surface. In some embodiments, the pushpin is mounted on the outer surface of the base. In some embodiments, the pushpin is configured to push an injection tool into a plant part. In some embodiments, one or more fastening elements are configured to mount the base on the plant part. In some embodiments, the inner surface is curved to approximately match the curvature of the plant part.

In another aspect, the document describes methods of using an injection system for plants. In some embodiments, the method includes bringing an inner surface of a base into contact with a part of a plant. In some embodiments, the method includes mounting the base on the part of a plant using one or more fasteners. In some embodiments, the method includes inserting at least a part of an injection tool into the part of a plant by pressing a button. In some embodiments, the method includes injecting a liquid composition into the part of a plant.

In some aspects, the present document provides plant injection systems comprising any of the chassis and an injection tool described herein. In some embodiments, the system further comprises a fluid delivery system. In some embodiments, the fluid delivery system is operatively connected to the injection tool. In some embodiments, the injection tool is an injection tool with a plurality of openings, and the system further comprises a fluid receiving system. In some embodiments, the fluid receiving system is in fluid communication with the injection tool with a plurality of openings.

Description of graphic materials

The present application may be understood by reference to the following description taken in conjunction with the accompanying figures.

Figs. 1, 2, 3A, 3B, 4A, and 4B illustrate several illustrative clamp-style chassis that accommodate an injection instrument.

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Fig. 5A-5C illustrate an illustrative pushpin style chassis housing that houses an injection tool.

Fig. 6A-6C illustrate an illustrative injection tool that may be housed in the chassis described herein.

Fig. 7A-7C illustrate an illustrative injection tool with multiple apertures that may be mounted on the chassis described herein.

Fig. 8A and 8B show illustrative plant injection systems.

Detailed description

The following description sets forth illustrative methods, parameters, systems, devices, and the like. However, it should be recognized that such description is not intended to limit the scope of the present invention, but is instead presented as a description of illustrative embodiments.

Wherever in this document the phrases for example, such as, including, etc. are used, the phrase and without limitation shall be understood to follow them unless expressly stated otherwise. Likewise, the terms example, illustrative, etc. are considered to be non-limiting.

The terms comprising, including, having, including (and similarly contains, includes, has, includes and other forms of these terms), etc. are used interchangeably and have the same meaning. In particular, each of these terms is defined in accordance with the generally accepted definition of the term comprising in the US patent law and is therefore interpreted as an open term meaning at least the following, and is also interpreted as not excluding other additional features, limitations, aspects, etc. Thus, for example, a process including steps a, b and c means that the process includes at least steps a, b and c.

Wherever terms are used in the singular, one or more are meant unless such interpretation would be meaningless in the context.

In some aspects, the present document provides various chassis suitable for integrating plant injection system components, such as injection tools. Such chassis are intended for use with various types of plants, including plants with thinner stems and/or less mature stems. In some embodiments, plant injection systems comprising a housing or chassis described herein may be suitable for use in small-scale plant injections, such as in nurseries. In some embodiments, plant injection systems comprising a housing or chassis described herein prevent leakage and damage to plants, especially for plants with thinner and/or less mature stems.

In other aspects, the present document also provides plant injection systems comprising such a chassis for introducing fluids, such as liquid formulations comprising one or more active ingredients, into a plant or a part of a plant. In some embodiments, the plant injection systems further comprise an injection tool. In some embodiments, the chassis houses the injection tool. In some embodiments, the chassis is connected to a fluid delivery system that comprises a source of a liquid formulation. In some embodiments, the plant injection systems are configured to deliver the liquid formulation from the fluid delivery system through the injection tool into a plant (e.g., a stem or thin trunk of a plant).

Chassis.

In certain aspects, various clamp-style chassis and pushpin-style chassis are provided that are configured to integrate an injection tool and components for fluidly connecting the injection tool to a fluid delivery system; and to install the injection tool and maintain it in place after installation on a plant.

Clamp style chassis.

In one aspect described in this document, a clamp-style chassis comprises a large lever, a small lever and a pressing element. In some embodiments, the large lever comprises a first gripping end, a first driving end and a first connecting portion between the first gripping end and the first driving end. In some embodiments, the small lever comprises a second gripping end, a second driving end and a second connecting portion between the second gripping end and the second driving end. In some embodiments, the pressing element connects the first driving end and the second driving end and is configured to push the first gripping end and the second gripping end toward each other. In some embodiments, the first connecting portion is larger than the second connecting portion. In some embodiments, the first connecting portion is connected to the second connecting portion to form a hinge. In some embodiments, the first gripping end, the second gripping end and the first connecting portion form a grip profile.

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In some embodiments, the injection tool is mounted on the first connecting section and inside the grip profile. In some embodiments, the injection tool is designed with the possibility of being introduced into a part of the plant in the same direction as the longitudinal axis of the chassis. In some embodiments, the injection tool is designed with the possibility of distributing the liquid composition into a part of the plant.

In some embodiments, when the chassis is in the relaxed mode, the first driving end and the second driving end are maximally distant from each other. In some embodiments, when the chassis is in the tense mode, the first driving end and the second driving end are closer to each other than in the relaxed mode, and the first gripping end and the second gripping end are further from each other than in the relaxed mode.

In some embodiments, the plant injection system is configured to operate in a pre-insertion configuration and a mounted configuration. In some embodiments, in the pre-insertion configuration, the plant part is positioned between the first gripping lever and the second gripping lever, but the injection tool is not inserted into the plant part. In some embodiments, in the mounted configuration, the plant part is positioned between the first gripping lever and the second gripping lever, and the injection tool is at least partially inserted into the plant part.

In some embodiments, the large lever and the small lever comprise a rigid material. In some embodiments, the rigid material comprises metal. In some embodiments, the biasing member is a spring. In some embodiments, the biasing member comprises metal, rubber, or silicone.

In another aspect, the document describes methods of using an injection system for plants. In some embodiments, the method includes squeezing the first driving end and the second driving end toward each other. In some embodiments, the method includes placing a plant part between the first gripping lever and the second gripping lever. In some embodiments, the method includes introducing at least a portion of the injection tool into the plant part by pushing the chassis toward the plant part in the same direction as the longitudinal axis of the chassis. In some embodiments, the method includes releasing the first driving end and the second driving end. In some embodiments, the method includes injecting a liquid composition into the plant part.

Fig. 1 shows an example of a clamp-style chassis (chassis 100), which includes a large lever 110 and a small lever 120. The large lever 110 has a gripping end 112, a connecting portion 114 and a driving end 116, and the small lever 120 has a gripping end 122, a connecting portion 124 and a driving end 126. A hinge 130 connects the connecting portions 114 and 124, and a pressing member 140 connects the driving ends 116 and 126. The gripping ends 112 and 122 and the connecting portion 114 form a gripping profile 150. An injection tool 160 is mounted on the connecting portion 114 inside the gripping profile 150. An arrow 170 indicates the longitudinal axis of the chassis 100.

In another aspect, the document describes a clamp-style chassis comprising a first lever, a second lever, a connecting portion, and a pressing element. In some embodiments, the first lever comprises a first gripping end and a first driving end. In some embodiments, the second lever comprises a second gripping end and a second driving end. In some embodiments, the connecting portion connects the first lever and the second lever between the gripping and driving ends. In some embodiments, the pressing element connects the first driving end and the second driving end and is configured to push the first gripping end and the second gripping end toward each other. In some embodiments, the first gripping end, the second gripping end, and the connecting portion form a gripping profile.

In some embodiments, the injection tool is mounted on the connecting section and inside the grip profile. In some embodiments, the injection tool is designed with the possibility of being introduced into a part of the plant in the same direction as the longitudinal axis of the chassis. In some embodiments, the injection tool is designed with the possibility of distributing the liquid composition into a part of the plant.

In some embodiments, when the chassis is in the relaxed mode, the first driving end and the second driving end are maximally distant from each other. In some embodiments, when the chassis is in the tense mode, the first driving end and the second driving end are closer to each other than in the relaxed mode, and the first gripping end and the second gripping end are further from each other than in the relaxed mode.

In some embodiments, the plant injection system is configured to operate in a pre-insertion configuration and a mounted configuration. In some embodiments, in the pre-insertion configuration, the plant part is positioned between the first gripping lever and the second gripping lever, but the injection tool is not inserted into the plant part. In some embodiments, in the mounted configuration, the plant part is positioned between the first gripping lever and the second gripping lever, and the injection tool is at least partially inserted into the plant part.

In some embodiments, the first lever, the second lever, and the connecting portion are made of a rigid material. In some embodiments, the rigid material comprises metal. In some embodiments, the pressing element is a spring. In some embodiments, the pressing element comprises metal, rubber, or silicone.

In another aspect, the document describes methods of using an injection system for plants. In some embodiments, the method includes squeezing the first driving end and the second driving end toward each other. In some embodiments, the method includes placing a plant part between the first gripping lever and the second gripping lever. In some embodiments, the method includes introducing at least a portion of the injection tool into the plant part by pushing the chassis toward the plant part in the same direction as the longitudinal axis of the chassis. In some embodiments, the method includes releasing the first driving end and the second driving end. In some embodiments, the method includes injecting a liquid composition into the plant part.

Fig. 2 shows an example of a clamp-style chassis (chassis 200), which includes a lever 210 and a lever 220. The lever 210 has a gripping end 212 and a driving end 214, and the lever 220 has a gripping end 222 and a driving end 224. A connecting portion 230 connects the levers 210 and 220, and a pressing member 240 connects the driving ends 214 and 224. The gripping ends 212 and 222 and the connecting portion 230 form a gripping profile 250. An injection tool 260 is mounted on the connecting portion 230 inside the gripping profile 150. An arrow 270 indicates the longitudinal axis of the chassis 200.

In another aspect, another clamp-style chassis is described in this document, comprising a first lever, a second lever, a clamp and a pressing element. In some embodiments, the first lever comprises a first pivot end and a first moving end. In some embodiments, the second lever comprises a second pivot end and a second moving end. In some embodiments, the clamp comprises a first side, a second side and a base that form a U-shape having an inner surface and an outer surface. In some embodiments, the first and second pivot ends are mounted on the outer surface of the clamp on the base. In some embodiments, the pressing element is connected to the outer surface of the clamp on the first side and the second side. In some embodiments, the pressing element is configured to push the first side and the second side toward each other.

In some embodiments, the injection tool is mounted on the inner surface of the clamp on the base. In some embodiments, the injection tool is designed to be inserted into a part of the plant in the same direction as the longitudinal axis of the chassis. In some embodiments, the injection tool is designed to distribute the liquid composition into a part of the plant.

In some embodiments, when the chassis is in a relaxed mode, the first driving end and the second driving end are maximally distant from each other. In some embodiments, when the chassis is in a tense mode, the first driving end and the second driving end are closer to each other than in a relaxed mode, and the first rotating end and the second rotating end cause the clamp to expand.

In some embodiments, the plant injection system is configured to operate in a pre-injection configuration and a set configuration. In some embodiments, in the pre-injection configuration, the plant part is positioned between the first side and the second side, but the injection tool is not inserted into the plant part. In some embodiments, in the set configuration, the plant part is positioned between the first side and the second side, and the injection tool is at least partially inserted into the plant part.

In some embodiments, the pressing member comprises metal or rubber. In some embodiments, the first side, the second side, and the base comprise plastic or silicone.

In another aspect, the document describes methods of using an injection system for plants. In some embodiments, the method includes squeezing a first driving end and a second driving end toward each other. In some embodiments, the method includes placing a part of a plant between a first side and a second side. In some embodiments, the method includes introducing at least a part of an injection tool into the part of a plant by pushing a chassis toward the part of the plant in the same direction as the longitudinal axis of the chassis. In some embodiments, the method includes releasing the first driving end and the second driving end. In some embodiments, the method includes injecting a liquid composition into the part of a plant.

In Fig. 3A and 3B, an example of a clamp-style chassis (chassis 300) is shown, which includes a lever 310 and a lever 320. The lever 310 has a pivot end 312 and a driving end 314, and the lever 320 has a pivot end 322 and a driving end 324. The side 332, the side 334, and the base 336 of the clamp 330 form a U-shape, which has an inner surface and an outer surface. The pressing member 340 connects the outer surface of the side 332 and the side 334. The pivot end 312 and the pivot end 322 are mounted on the outer surface of the base 336. The injection tool 350 is mounted on the inner surface of the base 336. The arrow 360 indicates the longitudinal axis of the chassis 300.

In another aspect, another clamp-style chassis is described herein, comprising a clamp and a fastening element. In some embodiments, the clamp comprises a first side, a second

- 5 047911 a side and a base that form a U-shape having an inner surface and an outer surface. In some embodiments, the first side has one or more openings and the second side has one or more corresponding openings. In some embodiments, the fastening element is designed to be inserted into one opening on the first side and a corresponding opening on the second side.

In some embodiments, the injection tool is mounted on the inner surface of the clamp on the base. In some embodiments, the injection tool is designed with the possibility of being introduced into a part of the plant in the same direction as the longitudinal axis of the chassis. In some embodiments, the injection tool is designed with the possibility of distributing the liquid composition into a part of the plant.

In another aspect, the document describes methods for using an injection system for plants. In some embodiments, the method includes placing a plant part between a first side and a second side. In some embodiments, the method includes introducing at least a portion of an injection tool into the plant part by pushing a chassis toward the plant part in the same direction as the longitudinal axis of the chassis. In some embodiments, the method includes introducing a fastener into one opening on the first side and a corresponding opening on the second side to clamp the plant part. In some embodiments, the method includes injecting a liquid composition into the plant part.

In Fig. 4A and 4B, an example of a clamp-style chassis (chassis 400) is shown, which includes a clamp 410 and a fastener 420. The clamp 410 has a side 430, a side 440 and a base 450. The side 430 has openings 432, and the side 440 has corresponding openings (not shown in the figure). The side 430, the side 440 and the base 450 of the clamp 410 form a U-shape, which has an inner surface and an outer surface. An injection tool 460 is mounted on the inner surface of the base 450. An arrow 470 indicates the longitudinal axis of the chassis 400.

Pushpin-style chassis

In one aspect, the document describes a pushpin-style chassis comprising a base, a pushpin, and one or more fastening elements. In some embodiments, the base has an inner surface and an outer surface. In some embodiments, the pushpin is mounted on the outer surface of the base. In some embodiments, the pushpin is configured to push an injection tool into a plant part. In some embodiments, one or more fastening elements are configured to mount the base on the plant part. In some embodiments, the inner surface is curved to approximately match the curvature of the plant part.

In some embodiments, the injection tool is mounted on the inner surface of the base and is configured to penetrate into a part of the plant and distribute the liquid composition into the part of the plant.

In another aspect, the document describes methods of using an injection system for plants. In some embodiments, the method includes bringing an inner surface of a base into contact with a part of a plant. In some embodiments, the method includes mounting the base on the part of a plant using one or more fasteners. In some embodiments, the method includes inserting at least a part of an injection tool into the part of a plant by pressing a button. In some embodiments, the method includes injecting a liquid composition into the part of a plant.

Fig. 5A-5C illustrate an example of a pushpin style chassis (chassis 500) that includes a base 510, a pushpin 520, and a fastener 530. The base 510 has an inner surface 512 and an outer surface 514. An injection tool 540 is mounted on the inner surface 512 of the base 510.

Injection instruments.

Any injection instruments compatible with the clip-on and pushpin-style chassis described herein may be used. Suitable injection instruments are described, for example, in WO 2020/021041 (see pp. 32-38 and Figs. 14-22) and WO 2021/152093 (see pp. 52-64 and Figs. 34-41 and 68-76), which are incorporated herein by reference.

In some embodiments, the injection tools comprise a tool body, at least a portion of which is designed to be inserted into a plant, such as a stem or trunk of a plant. The tool body has a channel system (having one or more channels) through which a fluid can flow, ending in an inlet through which the fluid enters the injection tool, and one or more distribution holes through which the fluid is delivered into the plant. In some embodiments, the insertion portion of the tool has a size and shape such that when inserted into a plant, damage to the target plant is minimized, while maintaining the effective functionality of the injection tool in terms of delivering the desired dose of the liquid composition during the desired period of time directly into the active vascular network of the plant.

In some embodiments, the selected injection tools enable precise delivery (also referred to as precise injection) of the composition into the plant. Precise delivery means delivering the composition only or substantially only to a target site on the plant. For example, in some embodiments, the target site is the active vasculature of a tree. In some embodiments, the active vasculature of a tree is xylem and/or phloem. In other embodiments, precise delivery of the liquid composition includes inserting the injection tool so that the distribution reservoir is located in the active vasculature of the plant and no further.

In some embodiments, the injection tool is small enough to fit on the chassis described herein. In some embodiments, the length of the injection tool is less than any of the following: 3, 4, 5, 6, 7, 8, 9, 10, 15, 20, 25, 30, 35, 40, 45, 50, 60, 70, 80, 90 or 100 mm. In some embodiments, the length of the injection tool is less than 20 mm.

In some embodiments, the length of the injection tool is equal to the length along the longitudinal axis of the injection tool. In some embodiments, the longitudinal length of the injection tool is equal to the length along the penetration direction of the injection tool.

In some embodiments, the length of the exposed or visible portion of the injection instrument when the injection instrument is positioned on the chassis (e.g., the visible portion of injection instruments 160 in Fig. 1, 260 in Fig. 2, 350 in Fig. 3B, 460 in Fig. 4B, and 540 in Fig. 5C) is less than any of the following: 1.5, 2, 2.5, 3, 3.5, 4, 4.5, 5, 7.5, 10, 12.5, 15, 17.5, 20, 25, 30, 35, 40, 45, or 50 mm. In some embodiments, the length of the exposed or visible portion of the injection instrument when the injection instrument is positioned on the chassis is less than 2.5 mm.

By having a penetrating structure, or a penetrating element, the injection tool can be introduced, for example, screwed, pushed or hammered, into the plant without first forming a receiving recess. More precisely, the injection tool according to the present invention more or less automatically forms the necessary opening when moving into the plant. Therefore, it can be introduced into the plant in a single work step. Thus, the injection tool makes it possible to reduce the amount of work to be performed, which can make the overall process significantly more efficient.

In another embodiment, the penetrating element comprises a wedge-shaped profile of the housing (for example, tapering from the near part to the far part, for example, a spike, a nail, a wedge, or the like). The wedge-shaped profile of the housing allows the injection tool to be effectively advanced into the plant, for example, by hammering or driving. In this example, the injection tool comprises a penetrating distribution housing, which comprises one or more of a wedge or rod profile and has a conical shape. For example, the penetrating distribution housing is conical and comprises external longitudinal grooves or recesses (for example, examples of distribution tanks). The injection tool comprises a striking head (optionally as a base element). Optionally, the conical part of the penetrating distribution housing is closer to the base than the part in the form of a nail tip (or a cutting element).

In another embodiment, the injection tool comprises a wedge-shaped portion (a wedge-shaped profile of the housing) in the form of a spearhead, provided with a penetrating element comprising a leading end or a cutting edge at its leading edge or distal portion. In such a condition, the term leading edge may refer to the distal portion of the penetrating distribution housing directed toward the plant and interacting with the plant during penetration. This exemplary wedge-shaped profile of the housing is an alternative wedge-shaped profile that allows the injection tool to be effectively advanced into the plant. In particular, the wedge-shaped profile of the housing opens the plant by pushing apart the cut plant tissue. In some examples, the wedge-shaped profiles of the housing gain access to the interior of the plant with minimal damage (e.g., with little or no damage) to the internal structure of the plant. For example, pushing apart by the wedge-shaped profile of the housing leaves the structure of the transport of liquids of the plant, such as capillaries or the like, minimally damaged (including undamaged or minimally damaged).

In one example, the wedge-shaped profile of the body comprises a flat spearhead or a leading edge comprising two or more lateral wing-shaped portions. The wedge-shaped profile of the body is optionally selected based on the plant to be processed. For example, the strength of the wedge-shaped profile of the body is adapted to the intended application or plant. Depending on the plant, the cutting element is selected with a wedge in the form of a nail head (for example, a round or conical wedge) or a wedge-shaped profile of the body comprising two or more wings extending from the leading edge of the penetrating distribution body.

Optionally, in some embodiments, the penetrating distribution body comprising one or more profiles described herein comprises one or more openings, such as distribution reservoirs. The distribution reservoirs provide one or more spaces, cavities, recesses, pockets, or the like within the plant when injected.

- 7 047911 of a tool. Distribution reservoirs facilitate the distribution of liquid formulations, for example, by retaining the formulations in the cavities of the reservoirs and while providing interaction of the formulations with plant tissue. In other examples, the distribution reservoirs comprise distribution channels that facilitate the delivery of liquid formulations within the channels and along the profile of the housing, which is a penetrating distribution housing. The injection tools described herein comprise one or more inlet passages and distribution openings associated therewith. Optionally, the injection tools comprise a plurality of inlet passages that deliver liquid formulations to a plurality of distribution openings. For example, the injection tools comprise at least one inlet passage or passages, each of which terminates in at least one distribution opening. Optionally, the injection tools comprise one or more of the previously described distribution reservoirs (sometimes referred to as apertures), and the distribution openings are open into the distribution reservoirs.

The injection tools described herein comprise a channel (e.g., an inlet passage) and at least one dispensing orifice connected to the channel. The inlet passages (or channels) provide a distributed network of outlets, or dispensing orifices, for the injection tool to efficiently distribute a liquid formulation of the active ingredient to one or more locations within a plant.

At least one dispensing orifice extends from the main channel. At least one dispensing orifice (also called an outlet channel) facilitates delivery of the liquid formulation of the active ingredient to one or more locations relative to the injection instrument.

The distribution openings described in this document (alternatively, the outlet channels) are oriented transversely relative to the direction of penetration of the injection instruments (e.g., corresponding to the longitudinal axis of the body of the instruments). For example, the transverse distribution openings are oriented at an angle relative to the direction of penetration. In one example, the distribution openings are oriented so that they open at an angle of 90 degrees relative to the direction of movement during penetration. In another example, the distribution openings are oriented at angles from about 100 to 180 degrees relative to the direction of movement during penetration (e.g., the longitudinal axis of the body of the injection instruments). In some embodiments, the distribution openings are oriented at angles from about 90 to 180 degrees relative to the direction of movement during penetration.

In connection with the orientation of the dispensing openings, one or more of the following three directions are considered: the direction of insertion, the direction of movement during penetration, and the direction of release (or distribution). In one example, the direction of insertion is the general direction in which the injection tool is inserted or advanced into the plant (e.g., from the outside of the plant to the inside). The direction of insertion generally coincides with the axis of the injection tool, such as the longitudinal axis of the housing described herein. Accordingly, when discussing the orientation of the dispensing openings, the longitudinal axis of the housing is used as a reference location corresponding to the direction of insertion.

The direction of movement during penetration is the direction in which the injection tool should be moved (e.g. rotated) to penetrate the plant for the purpose of injection. In embodiments of injection tools comprising cutting elements in the form of a drill or threaded part, the direction of movement during penetration runs along the thread of the drill or screw part (and in some examples is indicated by a circle and a dot in the figures, indicating the direction of movement during penetration behind the plane and from the plane of the leaf). Distribution holes, for example, provided between the threads, extend in a transverse orientation relative to the direction of movement during penetration in addition to the direction of introduction. In embodiments of the injection tool herein with wedge-shaped profiles of the body, which are tapped or driven to penetrate the plant, such as injection tools with a nail-head part or a wedge-shaped part (e.g. with exemplary wedge-shaped profiles), the direction of movement during penetration corresponds to the direction of introduction. In each of the embodiments described herein (e.g., rod-shaped or wedge-shaped profiles), one or more dispensing openings are transverse (have a direction of release or opening at a different angle) to the longitudinal axis of the housing and the corresponding direction of insertion. In addition, in exemplary embodiments comprising a rod-shaped profile, for example, comprising threads or cutting elements such as a drill, one or more dispensing openings (e.g., a direction of release or a direction of opening of the openings) are transverse (directed at a different angle) to the direction of movement during penetration. Accordingly, each of the direction of insertion and the direction of movement during penetration are collectively referred to as penetration directions, and the directions of release or opening of the dispensing openings of the injection instruments are transverse

- 8 047911 to the corresponding penetration directions according to the embodiments.

In some examples, the opening direction or the discharge direction of one or more dispensing openings extends backward relative to the direction of penetration, including the direction of movement during penetration described herein. The term extending backward or the like in this context refers to an extension of at least one dispensing opening in a reverse or opposite orientation relative to the direction of movement during penetration. The term extending backward is not limited to an extension opposite to the direction of movement during penetration, i.e., a backward direction in the narrow sense, but rather an extension at an angle to the direction of movement during penetration, other than a right angle or greater. For example, in some examples, the dispensing openings are oriented at an angle of 105 degrees relative to the direction of movement during penetration.

In another example, by orienting one or more dispensing openings at an angle greater than 90 degrees relative to the direction of movement during penetration, clogging of the dispensing openings by plant tissue is minimized (e.g., eliminated or minimized). Accordingly, clogging is minimized and liquid formulations of active ingredients are delivered through one or more dispensing openings effectively and efficiently.

The injection tool 6001 shown in Figs. 6A-6C is an exemplary injection tool compatible with the clip-on and pushpin style chassis described herein. The injection tool 6001 is configured for use with less robust plants (e.g., softer trees, vines, stems, etc.), typically having a softer shell. For example, the previously described injection tools can have a length of 50 mm or more. In one example, these injection tools include penetrating distribution bodies (e.g., wedge or rod body profiles) with a length of 35 mm or more and a width of 30 mm or more. In contrast, the exemplary injection tools 6001 shown in Figs. 6A-6C, in some examples, have an overall length of about 5 mm or about 2.5 mm to 7.5 mm.

The injection tool 6001 has a wedge-shaped part 6020, such as a penetrating distributor body, having a wedge-type body profile. The injection tool 6001 comprises a striking head 6010 (an example of a base) and a wedge-shaped part 6020 (an example of a penetrating distributor body). The wedge-shaped part 6020 comprises a cutting element. For example, the wedge-shaped part 6020 comprises a cutting edge along a front surface 6021 directed away from the striking head 6010. The wedge-shaped part 6020 comprises penetrating and distributing elements, at least partially extending in one direction. For example, the penetrating element extends from the cutting edge along the front surface 6021 and near the distant part 6041 to the near part 6043 of the wedge-shaped part 6020. Similarly, the distributing element 6012 (containing the discharge channels 6027 and the distributing openings 6028) is located inside the penetrating element of the wedge-shaped part 6020. As shown in the side view of Fig. 6C, the penetrating element of the wedge-shaped part 6020 increases in thickness from the distant part 6041 to the near part 6043 and the striking head 6010.

The striking head 6010 optionally comprises a ribbed outer structure 6012, such as attachment projections, to facilitate the grip of the striking head 6010 and to securely connect the injection tool 6001 to the delivery device. A step is provided at the transition between the striking head 6010 and the wedge-shaped part 6020. The step forms an abutment surface 6013. The abutment surface 6013 extends relative to (for example, away from) the wedge-shaped part 6020. During the insertion of the injection tool 6001, the abutment surface 6013 comes into contact with the plant and stops further advancement of the injection tool 6001 into the plant. Compared to the abutment surfaces in other injection tools described in the present document, the abutment surface 6013 has a relatively larger size compared to the associated wedge portion 6020 (e.g., they have a similar size). The abutment surface 6013 of a larger size facilitates use with plants that are smaller in size and less robust, having a comparatively soft shell or boundary layer. The abutment surface of a relatively large size distributes the forces during injection over a correspondingly large surface 6013 and thereby minimizes trauma caused to the plant. The abutment surface 60313 additionally provides a protective surface for the injection tool 6001 for the purpose of establishing a strong bond with the plant.

As shown in Fig. 6B and 6C, the striking head 6010 comprises a connection opening 6011 (for example, an example of an inlet opening). The connection opening 6011 is in communication with the outlet channels 6027 and the distribution openings 6028, for example with the main channel 6025 (for example, an inlet passage). As shown in Fig. 6A and 6B, the outlet channels 6027 (for example, distribution openings) are in communication with the main channel 6025 and open in the transverse direction into the corresponding distribution openings 6028 (for example, distribution tanks).

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The fluid composition of the active ingredient is delivered from the outlet channels 6027 to the distribution openings 6028 transversely, for example relative to the longitudinal axis 6040 of the housing and the corresponding direction 6030 of introduction. The distribution openings 6028 keep the composition in the vicinity of the adjacent tissues of the plant. In the example shown in Fig. 6B, the outlet channels 6027 extend in the near direction to the striking head 6010 and transversely relative to the direction 6030 of introduction of the injection tool 6001. The main channel 6025 and the outlet channels 6027 form a channel system of the injection tool 6001.

The injection operation 6001 is at least in some respects similar to other injection instruments described herein. Due to the relatively small profile of the injection instrument 6001 (or smaller forms of other instruments), the injection instrument 6001 is easily inserted and positioned in relatively small plants or less robust plants containing softer plant material (e.g., tissue or the like). For example, the injection instrument 6001 is adapted to apply soft blows or manually press the instrument 6001 into a plant, such as a stem.

As shown in Fig. 6B, the injection tool 6001 is inserted along the insertion direction 6030 corresponding to the longitudinal axis 6040 of the body of the injection tool 6001. As the injection tool 6001 is advanced into the plant, the tool moves along the direction of movement 6031 during penetration, and in the example shown, the insertion direction 6030 corresponds to the direction of movement 6031 during penetration. Like other injection tools comprising a wedge-type body profile and described in this document, the wedge-shaped portion 6020 of the injection tool 6001 pushes the plant material apart as the tool 6001 is inserted into the plant. Pushing the plant material apart minimizes trauma to the plant material and, in some examples, promotes improved absorption of the formulations.

As further shown in Fig. 6B, the outlet channels 6027 (e.g., distribution openings) extend in a discharge direction 6032 to the distribution openings 6028 (e.g., distribution tanks). The discharge direction 6032 is transverse to the direction of movement 6031 during penetration (and the longitudinal axis 6040 of the housing). For example, the discharge direction 6032 is not coaxial with the direction of movement 6031 during penetration, the direction of introduction 6030 (together - the directions of penetration) and the longitudinal axis 6040 of the housing, passing at an angle of 125 degrees or the like. The transverse orientation of the outlet channels 6027 isolates the outlet channels 6027 from plant material that would otherwise enter the outlet channels during introduction. In addition, the distribution openings 6028 (e.g. distribution tanks) facilitate the placement of the outlet channels 6027 in a certain position within the housing profile, for example by deepening the channels 6027 relative to the outer part of the housing profile.

In other embodiments, injection tools with a plurality of openings can be used with the chassis or injection systems for plants described herein. In some embodiments, injection tools with a plurality of openings comprise a tool body, at least a portion of which is designed to be inserted into a plant trunk. The tool body has a channel system (having one or more openings) through which a fluid can flow, ending in one or more distribution openings and two or more access openings; the channel system provides fluid communication between the distribution openings and the access openings. While the description primarily describes a specific embodiment of an injection tool with a plurality of openings, one skilled in the art, based on this according to the present invention, can imagine a plurality of injection tools that can be modified as injection tools with a plurality of openings according to the present invention. For example, other injection tools described herein can be modified to include two access openings that are in fluid communication with the channel system providing fluid communication between the access openings and the distribution openings. Accordingly, the specific examples of multi-hole injection instruments provided herein are not limiting.

The present invention also provides plant injection systems for administering fluids, such as liquid formulations comprising one or more active ingredients (AI fluid), into a plant, comprising an injection tool with a plurality of openings. The plant injection systems comprise a fluid delivery system, a fluid receiving system and a multi-opening injection tool, wherein the fluid delivery system is operatively connected to a first opening of the multi-opening injection tool, and the fluid receiving system is in fluid communication with a second opening of the multi-opening injection tool. The fluid delivery system facilitates a flow of fluid (as previously described in connection with other embodiments above) from a fluid delivery source through a system of channels in the multi-opening injection tip from a first access opening to a second access opening and to a dispensing opening(s) and hence into the plant. The fluid receiving system may have an open position in which fluid can flow through or be pumped out of the fluid receiving system, and a closed position in which fluid is retained in the fluid receiving system.

In some embodiments, the injection tool may have one, two, three or five dispensing openings. In some embodiments, each dispensing opening is directly connected to an access opening. In some embodiments, at least one dispensing opening is directly connected to an access opening. In some embodiments, at least one dispensing opening is directly connected to more than one access opening. In some embodiments, the connection between the access opening and the dispensing opening is curved. In some embodiments, the connection between the access opening(s) and the dispensing opening(s) is direct. In some embodiments, the connection between the access opening(s) and the dispensing opening(s) occurs through a system of internal channels (comprising a manifold of openings, a portion of the openings joining, and a delivery channel, as described below). In some embodiments, the injection tool with a plurality of openings comprises one or more access openings that are directly connected to one dispensing opening (e.g., without a manifold of openings, a portion of the openings joining, and a delivery channel).

In other embodiments, the injection instrument with multiple openings may have one, two, three, four, five or six distribution reservoirs. In some embodiments, the distribution reservoirs receive the active ingredient composition from a distribution opening connected to another distribution reservoir. In some embodiments, the distribution reservoirs receive the active ingredient composition from a distribution opening directly connected to the access opening. In some embodiments, the distribution reservoirs receive the active ingredient composition from a distribution opening connected to the access opening by a system of internal channels.

Fig. 7A-7C shows an illustrative example of an injection tool 74001 with a plurality of openings. In this example, the access openings 74022 are aligned with the penetration direction X. The distribution openings 74032 are located in the common wall of the distribution reservoirs 74034, near the cutting element 74012.

Fluid delivery systems.

A clothespin and pushpin style chassis that houses the injection tool is operatively connected to a fluid delivery system containing a liquid formulation. In some embodiments, the fluid delivery system and the source of the liquid formulation are integrated into a formulation cartridge, such as a pressurized container. In some embodiments, the formulation cartridge is a pressurized cylinder. In operation, the liquid formulation flows from the fluid delivery system through the injection tool into the plant. See, for example, WO 2020/021041 (see pages 17, 19, and 21-25, as well as Figs. 1 and 5-8) and WO 2021/152093 (see pages 3-6, 20, and 71-86, as well as Figs. 34, 38-41, and 57), which are incorporated herein by reference.

In some embodiments, the injection systems or components thereof that are used in the methods described herein are as shown in the figures. In some embodiments, these systems are adapted to administer a liquid formulation containing one or more active ingredients (including, for example, nutrients) to a plant or a part thereof. In some embodiments, such systems are mounted on a columnar part of a plant, such as a tree trunk.

In some embodiments, the methods presented herein include installing an injection tool into a stem, trunk, root, or branch of a plant, operatively connecting the injection tool to a fluid delivery system, and activating the fluid delivery system to initiate fluid flow from the fluid delivery system through the injection tool and into the plant. In some embodiments, two or more injection tools are installed into one or more of the stem, trunk, roots, branches, or the like of the plant to minimize injury to the plant (e.g., by minimizing the size of a single opening in the plant or the distance between tools along the plant). In some such embodiments, two or more injection tools are operatively connected to the same fluid delivery system. In some such embodiments, two or more injection tools are operatively connected to an independent fluid delivery system.

In some embodiments, the fluid delivery system comprises a spring-loaded fluid delivery system. In some embodiments of the above, the spring-loaded fluid delivery system is configured to operate at a pressure of 1.5 to 3 bar. In some embodiments, the fluid delivery system comprises a fluid delivery system comprising a pressurized container (e.g., a pressurized cylinder).

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In some exemplary embodiments, the spring-loaded fluid delivery system may have a base that holds one or more springs within one or more respective syringes. The design of the spring-loaded fluid delivery system may vary depending on the pressure, volume, time or other relevant parameters for delivering the liquid composition. For example, in some embodiments, the fluid delivery system may use multiple springs (e.g., two springs) to enable injection of a larger volume of the liquid composition. In some embodiments, a single spring may be used with a larger syringe, but this may affect the range of pressure used to inject the liquid composition.

In some embodiments, the delivery unit is designed as a metering pump with a pneumatic or hydraulic drive, configured to introduce a fluid composition (for example, a fluid medium containing one or more of a liquid, gas, gel, vapor, aerosol, or the like). Alternatively, the delivery unit is designed as a pneumatic or hydraulic delivery pump configured to provide one or more pressures. In some examples, in order to provide a gradual delivery of the composition to the plant under low pressure, the pressures created are close to atmospheric pressure, but exceeding it. In another example, the delivery unit provides a liquid composition in a passive manner, such as by means of hydrostatic pressure or capillary action. In one example, the delivery device is designed as a two-chamber unit, in which two chambers are located in a container, wherein one of these chambers contains a working medium under pressure, and the other contains a composition of the active ingredient, which can be displaced from the two-chamber unit through a valve by means of a working medium under pressure.

Fig. 8A shows an exemplary plant injection system 800 comprising a chassis 200 in which an injection tool 262 with a single opening is placed. The injection system 800 also comprises a fluid delivery system 802 operatively connected to the injection tool 262 via a delivery interface 804.

Fig. 8B shows an exemplary plant injection system 850 comprising a chassis 200 in which an injection tool 264 with a plurality of openings is accommodated. The injection system 850 also comprises a fluid delivery system 852 operatively connected to the injection tool 264 via a delivery interface 854. The injection system 850 further comprises a fluid receiving system 856 operatively connected to the injection tool 264 via a fluid receiving interface 858.

Applications of injection systems.

In some embodiments, this invention provides methods for improving or maintaining plant health using the injection systems described herein. In some embodiments, the present invention provides methods for treating diseased plants and/or methods for controlling bacteria, fungi, viruses and/or other pathogens that cause plant diseases. In further such embodiments, the present invention provides methods for treating plants whose xylem has been infected with disease-causing bacteria, fungi, viruses and/or other pathogens, controlling bacteria, fungi, viruses and/or other pathogens that cause disease, and preventing disease by preventing sufficient colonization of a plant by disease-causing pathogens such as bacteria, fungi and viruses.

In some embodiments, the method includes delivering a composition comprising one or more nutrients to a plant. In some embodiments, the method includes precisely delivering the composition to the plant. In some embodiments, precisely delivering the liquid composition includes inserting the injection instrument such that the distribution reservoir is located in and not beyond the active vasculature of the plant.

In some embodiments, the liquid composition is delivered to and not beyond the active vasculature of the plant when the injection tool is inserted into the columnar portion of the plant. In some embodiments, the liquid composition is delivered to and not beyond the active vasculature of the plant when the injection tool is inserted into the stem or trunk of the plant. In some embodiments, the liquid composition is delivered to and not beyond the xylem or phloem of the plant, or both, when the injection tool is inserted into the columnar portion of the plant. In one embodiment, the liquid composition is delivered to and not beyond the xylem or phloem of the plant, or both, when the injection tool is inserted into the stem or trunk of the plant.

In some embodiments, the methods deliver at least 50%, at least 60%, at least 70%, at least 80%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% of the liquid composition to the active vasculature of the plant. In some embodiments, the methods deliver at least 50%, at least 60%, at least 70%, at least 80%, at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% of the liquid composition to the xylem and/or phloem of the plant.

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In some embodiments, the method includes injecting a liquid composition into the vasculature through one or more sites on the columnar portion of the plant. In some embodiments, the method includes injecting a liquid composition into the vasculature through one or more sites on the trunk of the tree. In embodiments where the composition is administered through multiple injection sites, multiple injection systems described herein can be used. In some embodiments, when the composition is administered through multiple injection sites, the system comprises multiple injection instruments operatively connected to a single fluid delivery system.

The injection tools, injection systems and methods described herein generally provide one or more commercial advantages over tools, systems and methods currently known in the art. The advantages include one or more of the following: faster return to pre-infection yields, faster response (e.g., recovery), lower volumes of formulation required, lower loss of formulation to the environment, less damage to the tree, response in older trees, including trees over 100 years old, response in trees with significant disease symptoms (e.g., with 50% or less of the remaining canopy), and faster injection into trees.

The injection system according to the present invention is suitable for use with a wide variety of plants. Thus, the shape and dimensions of the injection tools used are advantageously adapted to the intended use. In some embodiments, the injection tool can be designed for use with relatively small or smaller plants. For example, injection tools intended for relatively small plants do not necessarily have a total length of about 3 mm to 20 mm, about 4 mm to 15 mm, or less than 10 mm.

In yet another aspect, the present document describes a method for modulating the phenotype of a plant or a plurality of plants, said method comprising the steps of (i) installing a plant injection system according to the present invention, provided herein, in a plant or a plurality of plants and (ii) using a liquid formulation of an active ingredient to modulate the phenotype of the plant.

In some embodiments, the active ingredient is selected from the group consisting of (i) pesticides and (ii) growth regulators. In some embodiments, the active ingredient is a biological compound or composition approved for use in food and feed.

Liquid compositions.

Any suitable liquid formulations may be used. In some embodiments, the liquid formulation is water-soluble. In some embodiments, the liquid formulation comprises nutrients. In some embodiments, the liquid formulation comprises micronutrients. In some embodiments, the liquid formulation is a semi-liquid formulation. In some embodiments, the liquid formulation is a gel-like formulation. In some embodiments, the liquid formulation is delivered as a semi-liquid or gel-like formulation.

The formulations are obtained, for example, by mixing the active ingredients with one or more suitable additives, such as suitable fillers, solvents, spreading agents, carriers, emulsifiers, dispersants, antifreeze agents, biocides, thickeners, adjuvants or the like. An adjuvant in this context is a component that enhances the biological effect of the formulation, wherein the component itself does not have a biological effect. Examples of adjuvants are agents that promote retention, spreading or penetration into the target plant. One embodiment of the present invention includes a sustained delivery of the active ingredient to the plant during the growing season, wherein the adjuvant is stabilizers, such as low-temperature stabilizers, preservatives, antioxidants, light stabilizers or other agents that improve chemical and/or physical stability.

Examples of typical formulations include water-soluble liquids (SL), emulsifiable concentrates (EC), emulsions in water (EW), suspension concentrates (SC, SE, FS, OD), water-dispersible granules (WG) and fluids (which include one or more of a liquid, gas, gel, vapour, aerosol, etc.). These and other possible formulation types are described, for example, by Crop Life International and in Pesticide Specifications, Guide to the Development and Use of FAO and WHO Pesticide Specifications, FAO Plant Production and Protection Series of the Joint FAO/WHO Meeting on Pesticide Specifications, 2004, ISBN: 9251048576; Catalogue of pesticide formulation types and international coding system, Technical Monograph No. 2, 6th Ed. May 2008, CropLife International.

Examples of suitable auxiliary substances are solvents, liquid carriers, surfactants, dispersants, emulsifiers, wetting agents, adjuvants, solubilizers, penetration enhancers, protective colloids, humectants, repellents, attractants, feeding stimulants, compatibilizers, bactericides, freezing point depressants, antifoams, colorants, stabilizers or nutrients, UV protection agents, tackifiers and/or binders.

Active ingredients.

In some embodiments, when using active ingredients, the application can be continuous over a longer period or at intervals. In some embodiments, the application can also be linked to a disease monitoring system and can be initiated on demand. In some embodiments, the formulations can contain from 0.5% to 90% by weight of the active compound, based on the weight of the formulation.

Numerous active ingredients can be used in injection systems compatible with the injection systems described in this document. The active ingredients referred to in this document under their common name are known and described, for example, in The Pesticide Manual (18th edition, ed. Dr. J A Turner (2018)), which includes, among others, herbicides, fungicides, insecticides, acaricides, nematicides, plant growth regulators, repellents, synergists, or can be found on the Internet (e.g. alanwood.net/pesticides).

In some embodiments, the active ingredient comprises a biologically active ingredient. In some embodiments, the active ingredient comprises phage, peptides, polypeptides, proteins, nucleic acids, or any combination thereof. In some embodiments, the active ingredient comprises RNA, DNA, or a combination thereof. In some embodiments, the active ingredient comprises small nuclear RNA (snRNA), microRNA (miRNA), small interfering RNA (siRNA), messenger RNA (mRNA), or a combination thereof. In some embodiments, the active ingredient comprises double-stranded RNA, such as siRNA. In some embodiments, the active ingredient comprises non-coding RNA, such as snRNA, miRNA, or siRNA, or any combination thereof.

In addition, the active ingredient may be selected from the following groups of compounds and compositions.

1. Fungicides.

1.1 Respiratory chain inhibitors.

1.1.1 Inhibitors of complex III at the Qo site, such as azoxystrobin, cumethoxystrobin, cumoxystrobin, dimoxystrobin, enestroburine, phenaminestrobin, phenoxystrobin/flufenoxystrobin, fluoxastrobin, kresoxim-methyl, metominostrobin, orysastrobin, picoxystrobin, pyraclostrobin, pyrametostrobin, pyraoxystrobin, trifloxystrobin, pyribencarb, triclopyricarb/chlordincarb, famoxadone and/or fenamidone.

1.1.2. Complex III inhibitors at the Qi site: cyazofamide and/or amisulbrom.

1.1.3. Complex II inhibitors: flutolanil, benodanil, bixafen, boscalid, carboxin, fenfuram, fluopyram, flutolanil, fluxapyroxad, furametpyr, isopyrazam, mepronil, oxycarboxin, penflufen, penthiopyrad, sedaxan, tecloftalam and/or tifluzamide.

1.1.4. Other respiratory chain inhibitors (e.g. complex I, uncouplers): diflumetrim.

1.1.5. Nitrophenyl derivatives: binapacryl, dinobuton, dinocap, fluazinam; ferimzone; organometallic compounds: fentin acetate, fentin chloride and/or fentin hydroxide; amethoctradine; and/or silthiofam.

1.2. Sterol biosynthesis inhibitors (SBI fungicides).

1.2.1. C14 demethylase inhibitors (DMI fungicides): triazoles: azaconazole, bitertanol, bromuconazole, cyproconazole, difenoconazole, diniconazole, diniconazole-M, epoxiconazole, fenbuconazole, fluquinconazole, flusilazole, flutriafol, hexaconazole, imibenconazole, ipconazole, metconazole, lobutanil, oxpoconazole, paclobutrazol , penconazole, propiconazole, prothioconazole, simeconazole, tebuconazole, tetraconazole, triadimefon, triadimenol, triticonazole and/or uniconazole;

1.2.2. Imidazoles: imazalil, pefurazoate, prochloraz, triflumizole; pyrimidines, pyridines and piperazines: fenarimol, nuarimol, pyrifenox, triforine; delta-14-reductase inhibitors: aldimorph, dodemorph, dodemorph-acetate, fenpropimorph, tridemorph, fenpropidin, piperalin, spiroxamine; 3-keto reductase inhibitors: fenhexamid.

1.3. Inhibitors of nucleic acid synthesis.

1.3.1. Phenylamides or acylamino acid fungicides: benalaxyl, benalaxyl-M, kiralaxyl, metalaxyl, ofuras, oxadixyl; other substances: hymexazole, octhilinone, oxolinic acid, bupirimate and/or 5-fluorocytosine.

1.4. Inhibitors of cell division and cytoskeleton.

1.4.1. Tubulin inhibitors: benzimidazoles, thiophanates: benomyl, carbendazim, fuberidazole, thiabendazole, thiophanate-methyl; triazolopyrimidines.

1.4.2. Cell division inhibitors: diethofencarb, ethaboxam, pencycuron, fluopicolide, zoxamide,

- 14 047911 metrafenone and/or pyriophenone.

1.5. Inhibitors of amino acid and protein synthesis.

1.5.1. Methionine synthesis inhibitors (anilinopyrimidines): cyprodinil, mepanipyrim, pyrimethanil; protein synthesis inhibitors: blasticidin-S, kasugamycin, kasugamycin hydrochloride hydrate, mildiomycin, streptomycin, oxytetracycline, polyoxin, validamycin A.

1.6. Signal transduction inhibitors.

1.6.1. MAP/histidine kinase inhibitors: fluoroimide, iprodione, procymidone, vinclozolin, fenpiclonil, fludioxonil; G-protein inhibitors: quinoxyfene.

1.7. Inhibitors of lipid and membrane synthesis.

1.7.1. Phospholipid biosynthesis inhibitors: edifenphos, iprobenfos, pyrazophos, isoprothiolane; lipid peroxidation: dicloran, quintozen, teknazen, tolclofos-methyl, biphenyl, chloroneb, etridiazole; phospholipid biosynthesis and cell wall formation: dimethomorph, flumorph, mandipropamid, pyrimorph, benthiavalicarb, iprovalicarb, valiphenalate.

1.7.2. Compounds that affect cell membrane permeability and fatty acids: propamocarb, propamocarb hydrochloride, fatty acid amide.

1.8. Multisite inhibitors.

1.8.1. Inorganic active substances Bordeaux mixture, copper acetate, copper hydroxide, copper oxychloride, basic copper sulfate, sulfur; thio- and dithiocarbamates: ferbam, mancozeb, maneb, metam, metiram, propineb, thiram, zineb, ziram; organochlorine compounds (e.g. phthalimides, sulfamides, chloronitriles): anilazine, chlorothalonil, captafol, captan, folpet, dichlofluanid, dichlorophene, hexachlorobenzene, pentachlorophenol and its salts, phthalide, tolylfluanid and other substances: guanidine, dodine, free base of dodine, guazatine, guazatine acetate, iminoctadine, iminoctadine triacetate, iminoctadine-tris(albesilate), dithianone.

1.9. Inhibitors of cell wall synthesis.

1.9.1. Glucan synthesis inhibitors: validamycin, polyoxin B; melanin synthesis inhibitors: pyroquiolone, tricyclazole, carpropamide, dicyclomet and/or fenoxanil.

1.10. Plant protection inductors.

1.10.1. Acibenzolar-N-methyl, probenazole, isothianil, tiadinil, prohexadione-calcium; phosphonates: fosetyl, fosetyl-aluminum, phosphoric acid and its salts.

1.11 Unclassified mechanism of action/classification unknown.

1.11.1. Bronopol, quinomethionate, cyflufenamide, cymoxanil, dazomet, debacarb, diclomesin, difenzoquat, difenzoquat-methylsulfate, diphenylamine, fenpyrazamine, flumetover, fluoxastrobin, flusulfamide, flutianil, fluxapyroxad, mefenoxam, metasulfocarb, nitrapyrine, nitrotalisopropyl, syn-copper, picarbutrazox, prothioconazole, proquinazide, pidiflumetofen (Adepidyn), pyraclostrobin, tebufloquin, teklofthalam, triazoxide and/or trifloxystrobin.

1.12. Antifungal agents for biological control: Ampelomyces quisqualis (e.g. AQ 10® from Intrachem Bio GmbH & Co. KG, Germany), Aspergillus flavus (e.g. AFLAGUARD® from Syngenta, Switzerland), Aureobasidium pullulans (e.g. BOTECTOR® from bio-ferm GmbH, Germany), Bacillus pumilus (e.g. NRRL accession no. B-30087 in SONATA® in BALLAD® Plus from AgraQuest Inc., USA), Bacillus subtilis (e.g. NRRL isolate no. B-21661 in RHAPSODY®, SERENADE® MAX and SERENADE® ASO from AgraQuest Inc., USA), Bacillus subtilis var. amyloliquefaciens FZB24 (e.g. TAEGRO® from Novozyme Biologicals, Inc., USA), Candida oleophila 1-82 (e.g. ASPIRE® from Ecogen Inc., USA), Candida saitoana (e.g. BIOCURE® (in a mixture with lysozyme) and BIOCOAT® from Micro Flo Company, USA (BASF SE) and Arysta), Chitosan (e.g. ARMOUR-ZEN from BotriZen Ltd., New Zealand), Clonostachys rosea f. catenulata, also called Gliocladium catenulatum (e.g. isolate J1446: PRESTOP® from Verdera, Finland), Coniothyrium minitans (e.g. CONTANS® e.g. Prophyta, Germany), Cryphonectria parasitica (e.g. Endothia parasitica from CNICM, France), Cryptococcus albidus (e.g. YIELD PLUS® from Anchor Bio-Technologies, South Africa), Fusarium oxysporum (e.g. BIOFOX® from S.I.A.P.A., Italy, FUSACLEAN® from Natural Plant Protection, France), Metschnikowia fructicola (e.g. SHEMER® from Agrogreen, Israel), Microdochium dimerum (e.g. ANTIBOT® from Agrauxine, France), Phlebiopsis gigantea (e.g. ROTSOP® from Verdera, Finland), Pseudozyma flocculosa (e.g. SPORODEX® from Plant Products Co. Ltd., Canada), Pythium oligandrum DV74 (e.g. POLYVERSUM® from Remeslo SSRO, Biopreparaty, Czech Republic), Reynoutria sachlinensis (e.g. REGALIA® from Marrone Bio-Innovations, USA), Talaromyces flavus V117b (e.g. PROTUS® from Prophyta, Germany), Trichoderma asperellum SKT-1 (e.g. ECO-HOPE® from Kumiai Chemical Industry Co., Ltd., Japan), T. atroviride LC52 (e.g. SENTINEL® from Agrimm Technologies Ltd, New Zealand), T. harzianum T-22 (e.g. PLANTSHTELD® from BioWorks Inc., USA), T. harzianum TH 35 (e.g. ROOT PRO® from Mycontrol Ltd., Israel), T. harzianum T-39 (e.g. TRICHODEX® and TRICHODERMA 2000® from Mycontrol Ltd., Israel and Makhteshim Ltd., Israel), T. harzianum and T. viride (e.g. TRICHOPEL from Agrimm Technologies Ltd, New Zealand), T.

- 15 047911 harzianum ICC012 and T. viride ICC080 (e.g. REMEDIER® WP from Isagro Ricerca, Italy), T. polysporum and/or T. harzianum (e.g. BINAB® from BINAB Bio-Innovation AB, Sweden), T. stromaticum (e.g. TRICOVAB® from C.E.P.L.A.C., Brazil), T. virens GL-21 (e.g. SOILGARD® from Certis LLC, USA), T. viride (e.g. TRIECO® from Ecosense Labs (India) Pvt. Ltd., India, BIO-CURE® F from T. Stanes & Co. Ltd., India), T. viride TV1 (e.g. T. viride TV1 from Agribiotec srl, Italy), Ulocladium oudemansii HRU3 (e.g. BOTRY-ZEN® from Botry-Zen Ltd, New Zealand), Beauveria bassiana PPRI 5339 (available from Becker Underwood as a BroadBand product), Metarhizium anisopliae FI1045 (available from Becker Underwood as a BioCane product), Metarhizium anisopliae var. acridum FI-985 (available from Becker Underwood as a GreenGuard product), and/or Metarhizium anisopliae var. acridum IMI 330189 (available from Becker Underwood as a Green Muscle product).

In some embodiments, the active ingredients may also comprise peptides, proteins or secondary metabolites. The term peptides, proteins or secondary metabolites refers to any compound, substance or by-product of a microorganism fermentation that has pesticidal activity. The definition includes any compound, substance or by-product of a microorganism fermentation that has pesticidal, including fungicidal, insecticidal or nematicidal activity. Examples of such proteins or secondary metabolites are Harpin (isolated from Erwinia amylovora, product known, for example, as Harp-N-Tek™, Messenger®, Employ™, ProAct™); and/or terpene constituents and a mixture of terpenes, i.e., α-terpinene, cymene and limonene (product known, for example, as Requiem® from Bayer CropScience LP, USA).

In some embodiments, useful proteins may also include antibodies to fungal target proteins or other proteins with antifungal activity, such as defensins and/or a proteinase inhibitor. Defensins may include, for example, NaD1, PhD1A, PhD2, Tomdef2, RsAFP2, RsAFP1, RsAFP3, and RsAFP4 from radish, DmAMP1 from dahlia, MsDefl, MtDef2, CtAMP1, PsD1, HsAFP1, VaD1, VrD2, ZmESR6, AhAMP1, and AhAMP4 from Aesculus hippocastanum, AflAFP from alfalfa, NaD2, AX1, AX2, BSD1, EGAD1, HvAMP1, JI-2, PgD1, SD2, SoD2, WT1, p139, and p1230 from pea. Proteinase inhibitors may include proteinase inhibitors from the following classes: serine, cysteine, aspartic and metalloproteinase and carboxypeptidase inhibitors such as StPin1A (US 7462695) or bovine trypsin inhibitor I-P.

2. Insecticidal compound.

2.1. Carbamate acetylcholinesterase inhibitors: aldicarb, alanycarb, bendiocarb, benfuracarb, butocarboxim, butoxycarboxim, carbaryl, carbofuran, carbosulfan, ethiofencarb, fenobucarb, formetanate, furathiocarb, isoprocarb, methiocarb, methomyl, metolcarb, oxamyl, pirimicarb, propoxur, thiodicarb, thiophanox, trimethacarb, XMC, xylylcarb and/or triazamate.

2.2. Organophosphorus acetylcholinesterase inhibitors: acephate, azamethiphos, azinphos-ethyl, azinphos-methyl, cadusafos, chlorethoxyphos, chlorfenvinphos, chlormephos, chlorpyrifos, chlorpyrifos-methyl, coumaphos, cyanophos, demeton-^-methyl, diazinon, dichlorvos/DDVP, dicrotophos, dimethoate, dimethylvinphos, disulfoton, EPN, ethion, ethoprofos, famphur, fenamiphos, fenitrothion, fenthion, fosthiazate, heptenophos, imiciafos, isofenphos, isopropyl-O-(methoxyaminothiophosphoryl)salicylate, isoxathion, malathion, mecarbam, methamidophos, methamidophos, mevinphos, monocrotophos, nalad, omethoate, oxydemeton-methyl, parathion, parathion-methyl, phentoate, phorate, fosalone, phosmet, phosphamidon, phoxim, pirimiphos-methyl, profenofos, propetamphos, prothiophos, pyraclofos, pyridafention, quinalphos, sulfotep, tebupirimphos, temephos, terbufos, tetrachlorvinphos, thiometone, triazophos, trichlorfon and/or vamidothion.

2.3. Antagonists of GABA-dependent chloride channels.

2.4 Cyclodiene organochlorines: endosulfan; or M-2.B fiproles (phenylpyrazoles): ethiprole, fipronil, flufiprole, pyrafluprole or pyriprole.

2.5. Sodium channel modulators from the pyrethroid class: acrinathrin, allethrin, d-cis-transallethrin, d-trans-allethrin, bifenthrin, bioallethrin, bioallethrin-^-cyclopentenyl, bioresmethrin, cycloprothrin, cyfluthrin, beta-cyfluthrin, cyhalothrin, lambda-cyhalothrin, gamma-cyhalothrin, cypermethrin, alpha-cypermethrin, beta-cypermethrin, theta-cypermethrin, zeta-cypermethrin, cyphenothrin, deltamethrin, momfluorothrin, empenthrin, esfenvalerate, etofenprox, fenpropathrin, fenvalerate, flucythrinate, flumethrin, tau-fluvalinate, halfenprox, imiprothrin, meperfluthrin, metofluthrin, permethrin, phenothrin, prallethrin, profluthrin, pyrethrin (pyrethrum), resmethrin, silafluofen, tefluthrin, tetramethylfluthrin, tetramethrin, tralomethrin, transfluthrin, DDT and/or methoxychlor.

2.6. Nicotinic acetylcholine receptor agonists from the neonicotinoid class: acetamiprid, clothianidin, cycloxapride, dinotefuran, flupyradifurone, imidacloprid, nitenpyram, sulfoxaflor, thiacloprid and/or thiamethoxam.

2.7. Activators of allosteric nicotinic acetylcholine receptors from the spinosyn class: spinosad, spinetoram.

2.8. Chloride channel activators from the mectin class: abamectin, emamectin benzoate, ivermectin

- 16 047911 tin, lepimectin and/or milbemectin.

2.9. Juvenile hormone mimetics: hydroprene, kinoprene, methoprene, fenoxycarb and/or pyriproxyfen.

2.10. Non-specific multisite inhibitors: methyl bromide and other alkyl halides, chloropicrin, sulfuryl fluoride, borax and/or potassium antimonyl tartrate.

2.11. Selective inhibitors of homoptera feeding: pymetrozine, flonicamid and/or pyrifluquinazone.

2.12. Mite growth inhibitors: clofentezine, hexythiazox, diflavin and/or etoxazole.

2.13. Mitochondrial ATP synthase inhibitors: diafenthiuron, azocyclotin, cyhexatin, fenbutatin oxide, propargite and/or tetradifon.

2.14. Oxidative phosphorylation uncouplers: chlorfenapyr, DNOC and/or sulfluramide; M-13 nicotinic acetylcholine receptor channel blockers: bensultap, cartap hydrochloride, thiocyclam and/or thiosultap sodium.

2.15. Chitin biosynthesis inhibitors type 0 (benzoylurea class): bistrifluron, chlorfluazuron, diflubenzuron, flucycloxuron, flufenoxuron, hexaflumuron, lufenuron, novaluron, noviflumuron, teflubenzuron and/or triflumuron.

2.16. Chitin biosynthesis inhibitors type 1: buprofezin.

2.17. Compounds that disrupt moulting: cyromazine.

2.18. Ecdysone receptor agonists: methoxyfenozide, tebufenozide, halofenozide, fufenozide and/or cromagnezoide.

2.19. Octopamine receptor agonists: amitraz.

2.20. Mitochondrial complex III electron transport inhibitors: hydramethylnon, acequinocil, flomethoquine, fluacrypyrim and/or pyriminostrobin.

2.21. Mitochondrial complex I electron transport inhibitors: fenazaquine, fenpyroximate, pyrimidifen, pyridaben, tebufenpyrad, tolfenpyrad, flufenerim and/or rotenone.

2.22. Voltage-gated sodium channel blockers: indoxacarb and/or metaflumizone.

2.23. Lipid synthesis inhibitors, acetyl-CoA carboxylase inhibitors: spirodiclofen, spiromesifen and/or spirotetramat.

2.24. Mitochondrial complex II electron transport inhibitors: cyenopyrafen, cyflumetofen and/or piflumide.

2.25 Ryanodine receptor modulators from the diamide class: flubendiamide, chlorantraniliprole (Rynaxypyr) and/or cyantraniliprole (Cyazypyr).

2.26 Others: aphidopyropene, imidacloprid, thiodicarb, clothianidin and/or abamectin;

2.27. Insecticides for biological control: Bacillus firmus (e.g. Bacillus firmus CNCM 1-1582, e.g. WO09126473A1 and WO09124707 A2, available commercially under the name Votivo) and/or 5-endotoxins from Bacillus thuringiensis (Bt).

3. Plant growth regulator.

3. 1. Antiauxins: clofibric acid and/or 2,3,5-triiodobenzoic acid.

3. 2. Auxins: 4-CPA, 2,4-D, 2,4-DB, 2,4-DEP, dichlorprop, fenoprop, IAA (indole-3-acetic acid), IBA, naphthaleneacetamide, α-naphthaleneacetic acid, 1-naphthol, naphthoxyacetic acid, potassium naphthenate, sodium naphthenate and/or 2,4,5-T.

3. 3. Cytokinins: 2iP, 6-benzylaminopurine (6-BA), 2,6-dimethylpyridine and/or kinetin, zeatin.

3. 4. Defoliants: calcium cyanamide, dimethipin, endothal, merfos, metoxuron, pentachlorophenol, thidiazuron, tribufos and/or tributyl phosphorothritioate.

3.5 Ethylene modulators: aviglicin, 1-methylcyclopropene (1-MCP), prohexadione (prohexadione calcium) and/or trinexapac (trinexapac-ethyl).

3. 6. Ethylene release stimulants: ACC, etaselasil, ethephon, glyoxime; gibberellins: gibberellin, gibberellic acid.

3. 7. Growth inhibitors: abscisic acid, ancymidol, butralin, carbaryl, chlorphonium, chlorpropham, dikegulac, flumetralin, fluoridamide, fosamine, glyphosine, isopyrimol, jasmonic acid, maleic hydrazide, mepiquat (mepiquat chloride, mepiquat pentaborate), piproctanil, prohydrojasmone, profam and/or 2,3,5-triiodobenzoic acid.

3. 8. Morphactins: chlorflurene, chlorflurenol, dichloroflurenol and/or flurenol.

3. 9. Growth inhibitors: chlormequat (chlormequat chloride), daminozide, flurprimidol, mefluidide, paclobutrazol, tetsiclasis, uniconazole and/or metconazole.

3.1 0. Growth stimulants: brassinolide, forchlorfenuron and/or hymexazole.

3.1 1. Unclassified plant growth regulators/class unknown: amidochlor, benzofluor, buminafos, carvone, choline chloride, cyiobutide, clofencet, cloxiphonac, cyanamide, cyclanilide, cycloheximide, cyprosulfamide, epocoleon, ethylosate, ethylene, fenridazone, fluprimidol, fluthiacet, heptopargyl, golosulf, inabenfide, kinetin, caretazan, lead arsenate, metasulfocarb, polyamines, pydanone, salicylic acids, sintofen and/or triapentenol.

In one embodiment, the fungicidal compound is selected from the group of oxathiapiproline,

-

Claims (34)

dimoxystrobin, pyraclostrobin, azoxystrobin, trifloxystrobin, picoxystrobin, cyazofamid, boscalid, fluoxapyroxad, fluopyram, bixafen, isopyrazam, benzovindiflupyr, penthiopyrad, amethoctradine, difenoconazole, metconazole, prothioconazole, tebuconazole, propiconazole, cyproconazole, penconazole, myclobutanil, tetraconazole, hexaconazole, metrafenone, zoxamide, pyrimethanil, cyprodinil, metalaxyl, fludioxonil, dimethomorph, mandipropamide, tricyclazole, copper, metiram, chlorothalonil, dithianone, fluazinam, folpet, fosetyl-Al, captan, cymoxanil, mancozeb, kresoxim-methyl, orysastrobin, epoxiconazole, fluquinconazole, triticonazole, fenpropimorph and iprodione. In one embodiment, the plant growth regulator is selected from the group of 6-benzylaminopurine (=N6-benzyladenine), chlormequat (chlormequat chloride), choline chloride, cyclanilide, dikegulac, diflufenzopyr, dimethipine, ethephon, flumetralin, fluthiacet, forchlorfenuron, gibberellic acid, inabenfide, maleic hydrazide, mepiquat (mepiquat chloride), 1-methylcyclopropene (1-MCP), paclobutrazol, prohexadione (prohexadione calcium), prohydrojasmone, thidiazuron, triapentenol, tributylphosphorothritioate, trinexapac-ethyl or uniconazole. In another embodiment, the active ingredient is a biological control agent, such as a biopesticide. In some embodiments, compared to traditional synthetic chemical pesticides, biopesticides are non-toxic, safe to use, and can be highly specific. In some embodiments, they can be used as a preventive (or curative) tool to control diseases, nematodes and insects, as well as other pests. In some embodiments, biopesticides can reduce the use of traditional chemical-based pesticides without negatively affecting crop yields. The use of biological pesticides is compatible with use in food and feed production, and many of the biological agents are approved for use. This allows them to be used throughout the year in food production systems such as wine, banana, cocoa, coffee, as well as in fruit plantations, etc., where pest control is a major and growing problem. In one embodiment, the tools, systems and methods of the present invention are used in organic farming. In one embodiment, the active ingredients are ingredients that provide a systemic effect. Non-systemic active ingredients. In some embodiments, the active ingredients are non-systemic. Non-systemic active ingredients may be resistant to uptake by the plant or seedling vasculature, movement within the plant or seedling vasculature, or uptake by seeds when applied topically. Numerous non-systemic active ingredients may be used in injection systems compatible with the injection systems described herein. Penetrants. In some embodiments, penetrants that facilitate and/or enhance the uptake and distribution of the active ingredient in the target plant may be used in injection systems or injection instruments compatible with the injection systems described herein. Suitable penetrants in the context of the present invention include all such substances that are typically used to enhance the penetration of active agrochemical compounds into plants. Examples include alcohol alkoxylates such as coconut fat ethoxylate, isotridecyl ethoxylate, fatty acid esters such as rapeseed or soybean oil methyl esters, fatty amine alkoxylates such as tallowamine ethoxylate, or ammonium and/or phosphonium salts such as ammonium sulfate or diammonium hydrogen phosphate. CLAUSE OF INVENTION 1. A chassis (100) for introducing fluid media into a plant, configured to accommodate an injection tool, and wherein the chassis comprises: a large lever (110) comprising a first gripping end (112), a first driving end (116) and a first connecting portion (114) between the first gripping end and the first driving end; a small lever (120) comprising a second gripping end (122), a second driving end (126) and a second connecting portion (124) between the second gripping end and the second driving end; and a pressing element (140) connecting the first driving end (116) and the second driving end (126) and configured to push the first gripping end (112) and the second gripping end (122) towards each other, wherein the first connecting portion (114) is larger than the second connecting portion (124), wherein the first connecting portion (114) is connected to the second connecting portion (124) to form a hinge (130), wherein the first gripping end (112), the second gripping end (122) and the first connecting portion (114) form a grip profile (150), - 18 047911 wherein the first connecting section (114) is configured to receive an injection tool, wherein the chassis (100) is configured to introduce the injection tool into a part of the plant in the same direction as the longitudinal axis of the chassis, when pushing the chassis (100) in the direction of the part of the plant, wherein, when the chassis (100) is in the relaxed mode, the first driving end (116) and the second driving end (126) are maximally distant from each other, wherein, when the chassis (100) is in the tense mode, the first driving end (116) and the second driving end (126) are closer to each other than in the relaxed mode, and the first gripping end (112) and the second gripping end (122) are further from each other than in the relaxed mode. 2. The chassis (100) according to claim 1, characterized in that the large lever (110) and the small lever (120) contain a rigid material. 3. The chassis (100) according to item 2, characterized in that the rigid material contains metal. 4. A chassis (100) according to any one of claims 1-3, characterized in that the pressing element (140) is a spring. 5. A chassis (100) according to any one of claims 1-4, characterized in that the pressing element (140) contains metal, rubber or silicone. 6. An injection system for plants for introducing fluid media into a plant, comprising: an injection tool (160); and a chassis (100) according to any one of claims 1 to 5, wherein the injection tool (160) is configured to be mounted on the chassis (100). 7. The system according to claim 6, characterized in that the injection system for plants is designed with the possibility of operation in a configuration prior to introduction and a set configuration, wherein in the configuration prior to introduction, the part of the plant is located between the first gripping lever and the second gripping lever, but the injection tool is not inserted into the part of the plant, wherein in the set configuration, the part of the plant is located between the first gripping lever and the second gripping lever, and the injection tool is at least partially inserted into the part of the plant. 8. A chassis (200) for introducing fluid media into a plant, designed with the possibility of accommodating an injection tool, and wherein the chassis comprises: a first lever (210) comprising a first gripping end (212) and a first driving end (214); a second lever (220) comprising a second gripping end (222) and a second driving end (224); a connecting section (230) connecting the first lever (210) and the second lever (220) between the gripping and driving ends; and a pressing element (240) connecting the first driving end (214) and the second driving end (224) and configured to push the first gripping end (212) and the second gripping end (222) towards each other, wherein the first gripping end (212), the second gripping end (222) and the connecting portion (230) form a gripping profile (250), wherein the connecting portion (230) is configured to receive an injection tool, wherein the chassis (200) is configured to insert the injection tool into the plant part in the same direction as the longitudinal axis of the chassis, when pushing the chassis (200) in the direction of the plant part, wherein, when the chassis (200) is in a relaxed mode, the first driving end (214) and the second driving end (224) are maximally distant from each other, wherein, when the chassis (200) is in a tense mode, the first driving end (214) and the second driving end (224) are closer to each other than in the relaxed mode, and the first gripping end (212) and the second gripping end (222) are further apart than in the relaxed mode. 9. The chassis (200) according to claim 8, characterized in that the first lever (210), the second lever (220) and the connecting section (230) comprise a rigid material. 10. The chassis (200) according to claim 9, characterized in that the rigid material contains metal. 11. A chassis (200) according to any one of paragraphs 8-10, characterized in that the pressing element (240) is a spring. 12. The chassis according to paragraphs 8-11, characterized in that the pressing element (240) contains metal, rubber or silicone. 13. An injection system for plants for introducing fluid media into a plant, comprising: an injection tool (260); and a chassis (200) according to any one of claims 8 to 12, wherein the injection tool (260) is configured to be mounted on the chassis (200). 14. The system according to item 13, characterized in that the injection system for plants is made with - 19 047911 with the ability to operate in a configuration prior to insertion and an installed configuration, wherein in the configuration prior to insertion the plant part is located between the first gripping lever and the second gripping lever, but the injection tool is not inserted into the plant part, wherein in the installed configuration the plant part is located between the first gripping lever and the second gripping lever, and the injection tool is at least partially inserted into the plant part. 15. A chassis (300) for introducing fluid media into a plant, configured to accommodate an injection tool, and wherein the chassis comprises: a first lever (310) comprising a first rotary end (312) and a first moving end (314); a second lever (320) comprising a second rotary end (322) and a second driving end (324); a clamp (330) comprising a first side (332), a second side (334) and a base (336) which form a U-shape having an inner surface and an outer surface, wherein the first (312) and second (322) rotary ends are mounted on the outer surface of the clamp (330) on the base (336); and a pressing element (340) connected to the outer surface of the clamp (330) on the first side (332) and the second side (334) and configured to push the first side (332) and the second side (334) towards each other, wherein the inner surface of the clamp (330) on the base (336) is configured to receive an injection tool, wherein the chassis (300) is configured to introduce the injection tool into the part of the plant in the same direction as the longitudinal axis of the chassis, when pushing the chassis (300) in the direction of the part of the plant, wherein, when the chassis (300) is in the relaxed mode, the first driving end (314) and the second driving end (324) are maximally distant from each other, wherein, when the chassis (300) is in the tense mode, the first driving end (314) and the second driving end (324) are closer to each other than in the relaxed mode, and the first rotating end (312) and the second rotating end (322) causes the clamp (330) to expand. 16. The chassis (300) according to claim 15, characterized in that the pressing element (340) contains metal or rubber. 17. The chassis (300) according to claim 15 or 16, characterized in that the first side (332), the second side (334) and the base (336) contain plastic or silicone. 18. A plant injection system for introducing fluid media into a plant, comprising: an injection tool (350); and a chassis (300) according to any one of claims 15-17, wherein the injection tool (350) is designed to be mounted on the chassis (300). 19. The system according to claim 18, characterized in that the injection system for plants is designed to operate in a configuration prior to insertion and a set configuration, wherein, in the configuration prior to insertion, the part of the plant is located between the first side and the second side, but the injection tool is not inserted into the part of the plant, wherein, in the set configuration, the part of the plant is located between the first side and the second side, and the injection tool is at least partially inserted into the part of the plant. 20. A chassis (400) for introducing fluid media into a plant, configured to accommodate an injection tool, wherein the chassis (400) comprises: a clamp (410) comprising a first side (430), a second side (440) and a base (450) that form a U-shape having an inner surface and an outer surface, wherein the first side has one or more openings (432) and the second side has one or more corresponding openings; and a fastening element (420) configured to be inserted into one opening (432) on the first side (430) and a corresponding opening on the second side (440); wherein the inner surface of the clamp (410) on the base is designed with the possibility of receiving an injection tool, and the chassis (400) is designed with the possibility of introducing the injection tool into a part of the plant in the same direction as the longitudinal axis of the chassis, when pushing the chassis (400) in the direction of the part of the plant. 21. A chassis (500) for introducing fluid media into a plant, configured to accommodate an injection tool, and wherein the chassis (500) comprises: a base (510) having an inner surface (512) and an outer surface (514); a button (520) mounted on the outer surface of the base; and one or more fastening elements (530) configured to mount the base on a part of the plant, wherein the inner surface of the base is configured to receive an injection tool and the button (520) is configured to push the injection tool into the part of the plant to penetrate the part of the plant and distribute the liquid composition into the part of the plant. - 20 047911 22. The chassis (500) according to claim 21, characterized in that the inner surface (512) is curved to approximately match the curvature of the plant part. 23. A plant injection system for introducing fluid media into a plant, comprising: an injection tool (540); and a chassis according to any one of claims 20-22, wherein the injection tool (540) is designed to be mounted on the chassis (500). 24. The system of claim 6, 7, 13, 14, 18, 19 or 23, further comprising: a fluid delivery system operatively connected to the injection instrument. 25. The system according to claim 24, characterized in that the injection instrument is an injection instrument with multiple holes, and the system additionally comprises: a fluid receiving system, wherein the fluid receiving system is in fluid communication with an injection tool having a plurality of openings. 26. The system according to any one of claims 6, 7, 13, 14, 18, 19, 23, 24 and 25, characterized in that the longitudinal length of the open part of the injection instrument, when the injection instrument is placed on the chassis, is less than 2.5 mm. 27. A system according to any of paragraphs 6, 13, 18, 23, characterized in that the injection instrument has a wedge-shaped body profile. 28. The system according to any one of paragraphs 6, 13, 18, 23 or 27, characterized in that the injection instrument comprises: impact head (6010); a wedge-shaped portion (6020), wherein the wedge-shaped portion comprises a cutting edge along the front surface (6021) of the wedge-shaped portion directed away from the striking head (6010); a penetrating element extending from the cutting edge along the front surface (6021) of the wedge-shaped part; and a distributing element (6012) comprising outlet channels (6027) and distributing openings (6028) inside the penetrating element of the wedge-shaped part (6020). 29. The system according to claim 28, characterized in that the penetrating element of the wedge-shaped part (6020) increases in thickness from the distant part (6041) to the near part (6043) of the wedge-shaped part and the striking head (6010). 30. The system according to claim 28 or 29, further comprising a step at the transition between the striking head (6010) and the wedge-shaped part (6020), wherein the step forms a contact surface (6013) that extends away from the wedge-shaped part (6020), and wherein when the injection tool (6001) is inserted into the plant, the contact surface (6013) comes into contact with the plant and stops further advancement of the injection tool (6001) into the plant. 31. A method for introducing fluids into a plant using a chassis according to any one of claims 1-5 or an injection system for plants according to any one of claims 6, 7 and 24-30, wherein the method comprises: squeezing the first moving end and the second moving end towards each other; placing a part of a plant between the first gripping lever and the second gripping lever; introducing at least a portion of the injection tool into the plant portion by pushing the chassis toward the plant portion in the same direction as the longitudinal axis of the chassis; and releasing the first driving end and the second driving end. 32. A method for introducing fluids into a plant using a chassis according to any one of claims 8-12 or an injection system for plants according to any one of claims 13, 14 and 24-30, wherein the method comprises: squeezing the first moving end and the second moving end towards each other; placing a part of a plant between the first gripping lever and the second gripping lever; introducing at least a portion of the injection tool into the plant portion by pushing the chassis toward the plant portion in the same direction as the longitudinal axis of the chassis; and releasing the first driving end and the second driving end. 33. A method for introducing fluids into a plant using a chassis according to any one of claims 15-17 or a plant injection system according to any one of claims 18, 19 and 24-30, wherein the method comprises: squeezing the first moving end and the second moving end towards each other; placing a part of the plant between the first side and the second side; introducing at least a portion of the injection tool into the plant portion by pushing the chassis toward the plant portion in the same direction as the longitudinal axis of the chassis; and releasing the first driving end and the second driving end. 34. A method for introducing fluids into a plant using the chassis of claim 20 or the plant injection system of any one of claims 23-30, wherein the method comprises: placing a part of the plant between the first side and the second side; introducing at least a portion of the injection tool into the plant portion by pushing the chassis toward the plant portion in the same direction as the longitudinal axis of the chassis; and introducing the fastening element into one opening on the first side and a corresponding opening on the -