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DK3636753T5 - Fremgangsmåde til fremstilling af DNA-redigeret eukaryotisk celle - Google Patents

Fremgangsmåde til fremstilling af DNA-redigeret eukaryotisk celle Download PDF

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Publication number
DK3636753T5
DK3636753T5 DK18812837.5T DK18812837T DK3636753T5 DK 3636753 T5 DK3636753 T5 DK 3636753T5 DK 18812837 T DK18812837 T DK 18812837T DK 3636753 T5 DK3636753 T5 DK 3636753T5
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edited
eukaryotic cell
making dna
dna
making
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DK18812837.5T
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DK3636753T3 (da
Inventor
Tomoji Mashimo
Junji Takeda
Hiroyuki MORISAKA
Kazuto Yoshimi
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Univ Osaka
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
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    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/09—Fusion polypeptide containing a localisation/targetting motif containing a nuclear localisation signal
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
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    • C12N2800/00—Nucleic acids vectors
    • C12N2800/80—Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites

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  • Plant Pathology (AREA)
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  • Environmental Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Botany (AREA)
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  • Crystallography & Structural Chemistry (AREA)
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  • Pharmacology & Pharmacy (AREA)
  • Public Health (AREA)
  • Epidemiology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
DK18812837.5T 2017-06-08 2018-06-08 Fremgangsmåde til fremstilling af DNA-redigeret eukaryotisk celle DK3636753T5 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2017113747 2017-06-08
PCT/JP2018/022066 WO2018225858A1 (ja) 2017-06-08 2018-06-08 Ｄｎａが編集された真核細胞を製造する方法、および当該方法に用いられるキット

Publications (2)

Publication Number Publication Date
DK3636753T3 DK3636753T3 (da) 2024-05-06
DK3636753T5 true DK3636753T5 (da) 2024-08-26

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DK18812837.5T DK3636753T5 (da) 2017-06-08 2018-06-08 Fremgangsmåde til fremstilling af DNA-redigeret eukaryotisk celle

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US (3) US11807869B2 (da)
EP (2) EP3636753B1 (da)
JP (6) JP6480647B1 (da)
KR (1) KR102541398B1 (da)
CN (2) CN110770342B (da)
AU (1) AU2018279457B2 (da)
BR (1) BR112019025717A2 (da)
CA (1) CA3066599A1 (da)
DK (1) DK3636753T5 (da)
EA (1) EA201992795A1 (da)
ES (1) ES2980050T3 (da)
FI (1) FI3636753T3 (da)
MX (1) MX2019014497A (da)
PT (1) PT3636753T (da)
WO (1) WO2018225858A1 (da)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110770342B (zh) 2017-06-08 2023-11-17 国立大学法人大阪大学 Dna被编辑了的真核细胞的制造方法、和在该方法中使用的试剂盒
CN111247247A (zh) * 2017-08-21 2020-06-05 国立大学法人德岛大学 使用核苷酸靶识别的靶序列特异性改变技术
EP3660152A4 (en) * 2018-03-26 2020-07-15 National University Corporation Kobe University METHOD FOR MODIFYING A TARGET SITE IN DOUBLE-STRANDED DNA IN A CELL
US12227776B2 (en) 2018-06-13 2025-02-18 Caribou Biosciences, Inc. Engineered cascade components and cascade complexes
WO2019246555A1 (en) 2018-06-21 2019-12-26 Cornell University Type i crispr system as a tool for genome editing
WO2020122104A1 (ja) * 2018-12-11 2020-06-18 国立大学法人京都大学 ゲノムｄｎａに欠失を誘導する方法
KR20210149060A (ko) * 2019-03-07 2021-12-08 더 트러스티스 오브 컬럼비아 유니버시티 인 더 시티 오브 뉴욕 Tn7-유사 트랜스포존을 사용한 rna-유도된 dna 통합
WO2020184723A1 (ja) * 2019-03-14 2020-09-17 国立大学法人徳島大学 Ｃｒｉｓｐｒタイプｉ－ｄシステムを利用した標的配列改変技術
CN113646429A (zh) 2019-04-05 2021-11-12 国立大学法人大阪大学 敲入细胞的制作方法
CN114829607A (zh) * 2019-12-18 2022-07-29 因思科瑞普特公司 用于体内检测核酸引导的核酸酶编辑的细胞的Cascade/dCas3互补测定
US20230099483A1 (en) * 2020-01-24 2023-03-30 C4U Corporation Method for detecting specific dna in sample
US20230257768A1 (en) * 2020-06-12 2023-08-17 National Institute Of Advanced Industrial Science And Technology Poultry cell in which a gene encoding a protein of interest is knocked-in at egg white protein gene, and method for producing said poultry cell
JPWO2022097663A1 (da) 2020-11-06 2022-05-12
AU2022229417A1 (en) 2021-03-01 2023-10-05 C4U Corporation Cas3 protein
WO2022251465A1 (en) * 2021-05-26 2022-12-01 The Regents Of The University Of Michigan Crispr-cas3 systems for targeted genome engineering
CN113549650B (zh) * 2021-07-05 2023-05-09 天津协和生物科技开发有限公司 一种CRISPR-SaCas9基因编辑系统及其应用
CN115595330B (zh) * 2021-07-12 2024-08-02 中国科学院微生物研究所 一种CRISPR-Cas3系统及其在抗植物病毒方面的应用
WO2024204287A1 (ja) * 2023-03-27 2024-10-03 国立大学法人京都大学 修飾ヌクレオチドを有するｃｒＲＮＡ
WO2025023307A1 (ja) * 2023-07-26 2025-01-30 Ｃ４Ｕ株式会社 ガイドｒｎａおよびその利用

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201122458D0 (en) 2011-12-30 2012-02-08 Univ Wageningen Modified cascade ribonucleoproteins and uses thereof
US20140189896A1 (en) * 2012-12-12 2014-07-03 Feng Zhang Crispr-cas component systems, methods and compositions for sequence manipulation
JP2017512481A (ja) 2014-04-08 2017-05-25 ノースカロライナ ステート ユニバーシティーＮｏｒｔｈ Ｃａｒｏｌｉｎａ Ｓｔａｔｅ Ｕｎｉｖｅｒｓｉｔｙ Ｃｒｉｓｐｒ関連遺伝子を用いた、ｒｎａ依存性の転写抑制のための方法および組成物
KR102575342B1 (ko) * 2015-06-18 2023-09-05 더 브로드 인스티튜트, 인코퍼레이티드 표적외 효과를 감소시키는 crispr 효소 돌연변이
EP3348636B1 (en) * 2015-09-09 2021-12-01 National University Corporation Kobe University Method for modifying genome sequence that specifically converts nucleobase of targeted dna sequence, and molecular complex used in said method
EP3362571A4 (en) * 2015-10-13 2019-07-10 Duke University GENOM ENGINEERING WITH TYPE I CRISPR SYSTEMS IN EUKARYOTIC CELLS
US20190323038A1 (en) * 2016-06-17 2019-10-24 Montana State Univesity Bidirectional targeting for genome editing
CN110770342B (zh) 2017-06-08 2023-11-17 国立大学法人大阪大学 Dna被编辑了的真核细胞的制造方法、和在该方法中使用的试剂盒
CN111247247A (zh) * 2017-08-21 2020-06-05 国立大学法人德岛大学 使用核苷酸靶识别的靶序列特异性改变技术
CN107557378A (zh) * 2017-09-19 2018-01-09 安徽大学 一种基于I型CRISPR‑Cas系统中基因cas7‑3的真核基因编辑方法
CN107557373A (zh) * 2017-09-19 2018-01-09 安徽大学 一种基于I‑B型CRISPR‑Cas系统基因cas3的基因编辑方法
CN113528408B (zh) * 2021-06-08 2022-03-01 湖北大学 一种基于CRISPR-nCas3系统的高效基因组大片段删除方法及应用

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CA3066599A1 (en) 2018-12-13
WO2018225858A1 (ja) 2018-12-13
CN117778466A (zh) 2024-03-29
EP3636753B1 (en) 2024-04-03
JP6480647B1 (ja) 2019-03-13
US20240124898A1 (en) 2024-04-18
DK3636753T3 (da) 2024-05-06
US20240117381A1 (en) 2024-04-11
JP2019062923A (ja) 2019-04-25
EP4349973A2 (en) 2024-04-10
BR112019025717A2 (pt) 2020-09-01
ES2980050T3 (es) 2024-09-27
AU2018279457B2 (en) 2024-02-15
FI3636753T3 (fi) 2024-05-03
PT3636753T (pt) 2024-04-23
AU2018279457A1 (en) 2019-12-05
JP7301332B2 (ja) 2023-07-03
EP4349973A3 (en) 2024-06-26
KR102541398B1 (ko) 2023-06-07
JP2024028649A (ja) 2024-03-04
MX2019014497A (es) 2020-10-12
JP2019062921A (ja) 2019-04-25
JP7430358B2 (ja) 2024-02-13
EP3636753A1 (en) 2020-04-15
US11807869B2 (en) 2023-11-07
JP2023054185A (ja) 2023-04-13
JP2019062922A (ja) 2019-04-25
EP3636753A4 (en) 2021-03-31
JPWO2018225858A1 (ja) 2019-06-27
CN110770342B (zh) 2023-11-17
EA201992795A1 (ru) 2020-04-21
CN110770342A (zh) 2020-02-07
US20200102580A1 (en) 2020-04-02
KR20200015700A (ko) 2020-02-12

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