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DK362686A - ALKALIC PROTEASE, METHOD OF PRODUCING HYBRID VECTORS AND GENETICALLY TRANSFORMED MICROORGANISMS - Google Patents

ALKALIC PROTEASE, METHOD OF PRODUCING HYBRID VECTORS AND GENETICALLY TRANSFORMED MICROORGANISMS Download PDF

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Publication number
DK362686A
DK362686A DK362686A DK362686A DK362686A DK 362686 A DK362686 A DK 362686A DK 362686 A DK362686 A DK 362686A DK 362686 A DK362686 A DK 362686A DK 362686 A DK362686 A DK 362686A
Authority
DK
Denmark
Prior art keywords
protease
alkalic
genetically transformed
producing hybrid
hybrid vectors
Prior art date
Application number
DK362686A
Other languages
Danish (da)
Other versions
DK362686D0 (en
Inventor
Harald Berger
Werner Prof Goebel
Juergen Kreft
Friedhelm Bartnik
Original Assignee
Henkel Kgaa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=6277629&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DK362686(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Henkel Kgaa filed Critical Henkel Kgaa
Publication of DK362686D0 publication Critical patent/DK362686D0/en
Publication of DK362686A publication Critical patent/DK362686A/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

A structural gene which codes for the protease subtilisin Carlsberg or its fragments with proteolytic activity or variants with proteolytic activity, including leader sequences thereof, is described. Also described are hybrid plasmids and microorganism strains which contain this structural gene, and a process for separating these microorganism strains from inactive strains. <IMAGE>
DK362686A 1985-08-03 1986-07-30 ALKALIC PROTEASE, METHOD OF PRODUCING HYBRID VECTORS AND GENETICALLY TRANSFORMED MICROORGANISMS DK362686A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE19853527913 DE3527913A1 (en) 1985-08-03 1985-08-03 ALKALINE PROTEASE, METHOD FOR PRODUCING HYBRID VECTORS AND GENETICALLY TRANSFORMED MICROORGANISMS

Publications (2)

Publication Number Publication Date
DK362686D0 DK362686D0 (en) 1986-07-30
DK362686A true DK362686A (en) 1987-02-04

Family

ID=6277629

Family Applications (1)

Application Number Title Priority Date Filing Date
DK362686A DK362686A (en) 1985-08-03 1986-07-30 ALKALIC PROTEASE, METHOD OF PRODUCING HYBRID VECTORS AND GENETICALLY TRANSFORMED MICROORGANISMS

Country Status (7)

Country Link
EP (1) EP0214435B1 (en)
JP (1) JPS6232888A (en)
AT (1) ATE78868T1 (en)
AU (1) AU604640B2 (en)
DE (2) DE3527913A1 (en)
DK (1) DK362686A (en)
ZA (1) ZA865787B (en)

Families Citing this family (40)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK564086A (en) * 1986-11-25 1988-06-17 Novo Industri As ENZYMATIC DETERGENT ADDITIVE
WO1988006624A2 (en) * 1987-02-27 1988-09-07 Gist-Brocades N.V. Molecular cloning and expression of genes encoding proteolytic enzymes
JPH0787790B2 (en) * 1987-03-04 1995-09-27 三菱瓦斯化学株式会社 METHOD FOR PRODUCING NOVEL GENE DNA AND ALKALINE PROTEASE
JPH0783716B2 (en) * 1987-11-27 1995-09-13 昭和電工株式会社 Gene system encoding alkaline protease
DE3821491A1 (en) * 1988-06-25 1989-12-28 Henkel Kgaa HYBRID PLASMIDE FOR THE PRODUCTION OF SUBTILISIN CARLSBERG IN BACILLUS
DE3824827A1 (en) * 1988-07-21 1990-02-01 Henkel Kgaa PLASMIDES FOR GENERATING ALPHA AMYLASE IN BACILLUS
US5665587A (en) * 1989-06-26 1997-09-09 Novo Nordisk A/S Modified subtilisins and detergent compositions containing same
US5658871A (en) * 1989-07-07 1997-08-19 Lever Brothers Company, Division Of Conopco, Inc. Microbial lipase muteins and detergent compositions comprising same
ATE187490T1 (en) * 1989-08-25 1999-12-15 Henkel Research Corp ALKALINE PROTEOLYTIC ENZYME AND METHOD FOR PRODUCING
WO1991002803A1 (en) * 1989-08-25 1991-03-07 Henkel Research Corporation A method of suppressing chromosomal gene expression
DE3928046A1 (en) * 1989-08-25 1991-02-28 Henkel Kgaa HYBRID PLASMIDE FOR THE PRODUCTION OF SUBTILISIN CARLSBERG IN BACILLUS
US5482849A (en) * 1990-12-21 1996-01-09 Novo Nordisk A/S Subtilisin mutants
US5275945A (en) * 1991-10-08 1994-01-04 Vista Chemical Company Alkaline proteases stable in heavy-duty detergent liquids
DE4231726A1 (en) * 1992-09-23 1994-03-24 Cognis Bio Umwelt Mutated subtilisin-like serine proteases
US6300116B1 (en) 1996-11-04 2001-10-09 Novozymes A/S Modified protease having improved autoproteolytic stability
EP2278001B1 (en) 1996-11-04 2013-10-23 Novozymes A/S Protease variants and compositions
CN1272137A (en) 1997-08-29 2000-11-01 诺沃挪第克公司 Protease variants and compositions
AU8798198A (en) 1997-08-29 1999-03-22 Novo Nordisk A/S Protease variants and compositions
WO1999027082A1 (en) 1997-11-21 1999-06-03 Novo Nordisk A/S Protease variants and compositions
US6773907B2 (en) 1997-11-21 2004-08-10 Peter Kamp Hansen Subtilase enzymes
ATE504651T1 (en) 1998-12-18 2011-04-15 Novozymes As SUBTILASE ENZYMES OF THE I-S1 AND I-S2 SUBGROUPS WITH AN ADDITIONAL AMINO ACID RESIDUE IN AN ACTIVE LOOP REGION
DE60040285D1 (en) 1999-05-20 2008-10-30 Novozymes As SUBTILASE ENZYMES OF I-S1 AND I-S2 SUB-GROUPS WITH AT LEAST ONE ADDITIONAL AMINO ACID BETWEEN ITEM 127 AND 128
DE60039693D1 (en) 1999-05-20 2008-09-11 Novozymes As SUBTILASE ENZYMES OF I-S1 AND I-S2 SUB-GROUPS WITH AT LEAST ONE ADDITIONAL AMINO ACID RESISTANCE BETWEEN POSITIONS 125 AND 126
WO2000071687A1 (en) 1999-05-20 2000-11-30 Novozymes A/S Subtilase enzymes of the i-s1 and i-s2 sub-groups having at least one additional amino acid residue between positions 129 and 130
DE60040149D1 (en) 1999-05-20 2008-10-16 Novozymes As SUBTILASE ENZYMES OF I-S1 AND I-S2 SUB-GROUPS WITH AT LEAST ONE ADDITIONAL AMINO ACID RESISTANCE BETWEEN POSITIONS 126 AND 127
DE60040287D1 (en) 1999-05-20 2008-10-30 Novozymes As SUBTILASE ENZYMS OF I-S1 AND I-S2 SUB-GROUPS WITH AT LEAST ONE ADDITIONAL AMINO ACID BETWEEN POSITIONS 128 AND 129
DE60040282D1 (en) 1999-05-20 2008-10-30 Novozymes As SUBTILASE ENZYMES OF I-S1 AND I-S2 SUB-GROUPS WITH AT LEAST ONE ADDITIONAL AMINO ACID BETWEEN POSITIONS 132 AND 133
US6727085B2 (en) 1999-12-15 2004-04-27 Fanoe Tina Sejersgaard Subtilase variants having an improved wash performance on egg stains
US6777218B1 (en) 2000-03-14 2004-08-17 Novozymes A/S Subtilase enzymes having an improved wash performance on egg stains
ATE407202T1 (en) 2003-05-07 2008-09-15 Novozymes As ENZYME VARIANTS OF SUBTILISIN (SUBTILASES)
JP4880469B2 (en) 2003-10-23 2012-02-22 ノボザイムス アクティーゼルスカブ Protease with improved stability in detergents
BRPI0709978A2 (en) 2006-04-14 2011-08-02 Genencor Int one-step treatment of textile products
CN101460618A (en) 2006-04-20 2009-06-17 诺维信公司 Savinase variants having an improved wash performance on egg stains
MX2009011566A (en) 2007-04-30 2009-11-10 Danisco Us Inc Genencor Div Use of protein hydrolysates to stabilize metalloprotease detergent formulations.
SG148934A1 (en) 2007-06-11 2009-01-29 Novozymes As A process for combined biopolishing and bleach clean-up
US20130224757A1 (en) 2010-08-19 2013-08-29 Novozymes A/S Induced sporulation screening method
GB201212934D0 (en) * 2012-07-20 2012-09-05 Dupont Nutrition Biosci Aps Method
GB201212932D0 (en) * 2012-07-20 2012-09-05 Dupont Nutrition Biosci Aps Method
WO2020099490A1 (en) 2018-11-14 2020-05-22 Novozymes A/S Oral care composition comprising enzymes
JP2023539234A (en) 2020-08-24 2023-09-13 ノボザイムス アクティーゼルスカブ Oral care composition containing fructanase

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3557002A (en) * 1967-11-15 1971-01-19 Procter & Gamble Stabilized aqueous enzyme preparation
GB1240058A (en) * 1968-04-12 1971-07-21 Procter & Gamble Enzyme-containing detergent compositions
DK151269C (en) * 1978-07-04 1988-05-02 Novo Industri As PROTEINASE PREPARATION WITH REDUCED ALLERGENCY, PROCEDURE FOR PRODUCING IT AND MICRO-ORGANIC STORES FOR USE IN PRODUCING IT
DE2925427C2 (en) * 1979-06-23 1983-12-22 Henkel KGaA, 4000 Düsseldorf Process for the production of protease
DE3380878D1 (en) * 1982-11-01 1989-12-28 Miles Inc Method of heterologous cloning of gene in bacillus microorganism
IE81141B1 (en) * 1983-06-24 2000-04-05 Genencor Int Procaryotic carbonyl hydrolases
JPS6030685A (en) * 1983-07-29 1985-02-16 Lion Corp Recombinant dna, its preparation, bacillus subtilis containing the same, and preparation of protease using said bacillus subtilis
JPS6112287A (en) * 1984-06-26 1986-01-20 Lion Corp Recombinant dna, its preparation, bacterial strain containing same, preparation of exocytic secretion enzyme using same, and dna for promoting secretion of exocytic enzyme
US4617265A (en) * 1984-09-19 1986-10-14 Board Of Regents, University Of Texas System Colony blot assay for enterotoxigenic bacteria

Also Published As

Publication number Publication date
EP0214435B1 (en) 1992-07-29
AU6078986A (en) 1987-02-05
DE3686217D1 (en) 1992-09-03
ATE78868T1 (en) 1992-08-15
ZA865787B (en) 1987-03-25
EP0214435A3 (en) 1990-01-17
EP0214435A2 (en) 1987-03-18
DK362686D0 (en) 1986-07-30
AU604640B2 (en) 1991-01-03
JPS6232888A (en) 1987-02-12
DE3527913A1 (en) 1987-02-12

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