DK3494229T3 - Cellebaseret højkapacitetsscreening til aptamerer - Google Patents
Cellebaseret højkapacitetsscreening til aptamerer Download PDFInfo
- Publication number
- DK3494229T3 DK3494229T3 DK17790843.1T DK17790843T DK3494229T3 DK 3494229 T3 DK3494229 T3 DK 3494229T3 DK 17790843 T DK17790843 T DK 17790843T DK 3494229 T3 DK3494229 T3 DK 3494229T3
- Authority
- DK
- Denmark
- Prior art keywords
- aptamers
- cell
- based high
- throughout screening
- screening
- Prior art date
Links
- 108091023037 Aptamer Proteins 0.000 title 1
- 238000012216 screening Methods 0.000 title 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1048—SELEX
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6811—Selection methods for production or design of target specific oligonucleotides or binding molecules
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/16—Aptamers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/30—Chemical structure
- C12N2310/35—Nature of the modification
- C12N2310/351—Conjugate
- C12N2310/3517—Marker; Tag
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/30—Chemical structure
- C12N2310/35—Nature of the modification
- C12N2310/351—Conjugate
- C12N2310/3519—Fusion with another nucleic acid
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2320/00—Applications; Uses
- C12N2320/10—Applications; Uses in screening processes
- C12N2320/11—Applications; Uses in screening processes for the determination of target sites, i.e. of active nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2330/00—Production
- C12N2330/30—Production chemically synthesised
- C12N2330/31—Libraries, arrays
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/30—Oligonucleotides characterised by their secondary structure
- C12Q2525/301—Hairpin oligonucleotides
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Plant Pathology (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- Bioinformatics & Computational Biology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201662370599P | 2016-08-03 | 2016-08-03 | |
| PCT/IB2017/001113 WO2018025085A2 (en) | 2016-08-03 | 2017-08-03 | High throughput cell-based screening for aptamers |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK3494229T3 true DK3494229T3 (da) | 2024-01-29 |
Family
ID=60182820
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK17790843.1T DK3494229T3 (da) | 2016-08-03 | 2017-08-03 | Cellebaseret højkapacitetsscreening til aptamerer |
Country Status (25)
| Country | Link |
|---|---|
| US (2) | US11371077B2 (da) |
| EP (1) | EP3494229B1 (da) |
| JP (2) | JP7089502B2 (da) |
| KR (1) | KR102491566B1 (da) |
| CN (1) | CN110199030B (da) |
| AU (2) | AU2017306657B2 (da) |
| BR (1) | BR112019002085A2 (da) |
| CA (1) | CA3066654C (da) |
| DK (1) | DK3494229T3 (da) |
| EA (1) | EA201990423A1 (da) |
| ES (1) | ES2971123T3 (da) |
| FI (1) | FI3494229T3 (da) |
| HR (1) | HRP20240183T1 (da) |
| HU (1) | HUE065801T2 (da) |
| IL (1) | IL264490B2 (da) |
| LT (1) | LT3494229T (da) |
| MX (1) | MX2019001239A (da) |
| MY (1) | MY196527A (da) |
| PH (1) | PH12019500239A1 (da) |
| PL (1) | PL3494229T3 (da) |
| PT (1) | PT3494229T (da) |
| RS (1) | RS65228B1 (da) |
| SG (1) | SG11201900851QA (da) |
| SM (1) | SMT202400091T1 (da) |
| WO (1) | WO2018025085A2 (da) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3411081A4 (en) | 2016-02-02 | 2019-12-11 | Meiragtx UK II Limited | REGULATION OF GENE EXPRESSION BY APTAM-MODULATED POLYADENYLATION |
| RS65228B1 (sr) * | 2016-08-03 | 2024-03-29 | Meiragtx Uk Ii Ltd | Celularni skrining visoke propusnosti za aptamere |
| IL268819B2 (en) | 2017-02-21 | 2024-07-01 | Meiragtx Uk Ii Ltd | Regulation of gene expression by aptamer-mediated accessibility of polyadenylation signals |
| KR20200140805A (ko) | 2018-02-21 | 2020-12-16 | 브리스톨-마이어스 스큅 컴퍼니 | Camk2d 안티센스 올리고뉴클레오티드 및 그의 용도 |
Family Cites Families (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1493825A3 (en) | 1990-06-11 | 2005-02-09 | Gilead Sciences, Inc. | Method for producing nucleic acid ligands |
| US5580737A (en) | 1990-06-11 | 1996-12-03 | Nexstar Pharmaceuticals, Inc. | High-affinity nucleic acid ligands that discriminate between theophylline and caffeine |
| US5270163A (en) | 1990-06-11 | 1993-12-14 | University Research Corporation | Methods for identifying nucleic acid ligands |
| US5567588A (en) | 1990-06-11 | 1996-10-22 | University Research Corporation | Systematic evolution of ligands by exponential enrichment: Solution SELEX |
| US6423493B1 (en) * | 1998-10-26 | 2002-07-23 | Board Of Regents The University Of Texas System | Combinatorial selection of oligonucleotide aptamers |
| US8008071B2 (en) * | 1999-11-08 | 2011-08-30 | University Of South Florida | Compositions and methods for detecting intracellular glucose and analogs thereof |
| US20040126882A1 (en) | 2000-06-15 | 2004-07-01 | Ellington Andrew D | Regulatable, catalytically active nucleic acids |
| EP1546733A2 (en) | 2002-09-27 | 2005-06-29 | University Of North Carolina At Chapel Hill | Methods and compositions for modification of splicing of pre-mrna |
| US20050136439A1 (en) * | 2003-09-12 | 2005-06-23 | North Carolina State University | Novel methods of inorganic compound discovery and synthesis |
| WO2006055351A2 (en) | 2004-11-08 | 2006-05-26 | Yale University | Structure-based compound design involving riboswitches |
| EP1851318A2 (en) * | 2005-02-09 | 2007-11-07 | BASF Plant Science GmbH | Expression cassettes for regulation of expression in monocotyledonous plants |
| US8367627B2 (en) * | 2005-09-15 | 2013-02-05 | Duke University | Focused libraries, functional profiling, laser SELEX, and DESELEX |
| CN101801185A (zh) * | 2007-03-22 | 2010-08-11 | 耶鲁大学 | 与控制可变剪接的核糖开关有关的方法和组合物 |
| JP2010528616A (ja) | 2007-05-29 | 2010-08-26 | イェール ユニバーシティー | オルタナティブスプライシングとｒｎａのスプライシングを調節するリボスイッチに関連する方法と組成物 |
| US8604176B2 (en) * | 2009-11-10 | 2013-12-10 | California Institute Of Technology | Protein-responsive RNA control devices and uses thereof |
| WO2013178674A1 (en) * | 2012-05-31 | 2013-12-05 | Novozymes A/S | Improved selection in fungi |
| LT2943579T (lt) * | 2013-01-10 | 2018-11-12 | Dharmacon, Inc. | Molekulių bibliotekos ir molekulių generavimo būdai |
| KR102699584B1 (ko) * | 2015-02-02 | 2024-08-28 | 메이라지티엑스 유케이 Ii 리미티드 | 대안적 스플라이싱의 압타머 매개 조절에 의한 유전자 발현 제어 |
| GB201506440D0 (en) | 2015-04-16 | 2015-06-03 | Univ Wageningen | Riboswitch-controlled screening and selection of desired biocatalysts |
| RS65228B1 (sr) * | 2016-08-03 | 2024-03-29 | Meiragtx Uk Ii Ltd | Celularni skrining visoke propusnosti za aptamere |
| US10011870B2 (en) * | 2016-12-07 | 2018-07-03 | Natera, Inc. | Compositions and methods for identifying nucleic acid molecules |
-
2017
- 2017-08-03 RS RS20240097A patent/RS65228B1/sr unknown
- 2017-08-03 JP JP2019505401A patent/JP7089502B2/ja active Active
- 2017-08-03 MY MYPI2019000618A patent/MY196527A/en unknown
- 2017-08-03 CN CN201780061565.2A patent/CN110199030B/zh active Active
- 2017-08-03 SG SG11201900851QA patent/SG11201900851QA/en unknown
- 2017-08-03 BR BR112019002085A patent/BR112019002085A2/pt active Search and Examination
- 2017-08-03 EA EA201990423A patent/EA201990423A1/ru unknown
- 2017-08-03 HR HRP20240183TT patent/HRP20240183T1/hr unknown
- 2017-08-03 MX MX2019001239A patent/MX2019001239A/es unknown
- 2017-08-03 PT PT177908431T patent/PT3494229T/pt unknown
- 2017-08-03 IL IL264490A patent/IL264490B2/en unknown
- 2017-08-03 US US16/322,865 patent/US11371077B2/en active Active
- 2017-08-03 EP EP17790843.1A patent/EP3494229B1/en active Active
- 2017-08-03 CA CA3066654A patent/CA3066654C/en active Active
- 2017-08-03 FI FIEP17790843.1T patent/FI3494229T3/fi active
- 2017-08-03 HU HUE17790843A patent/HUE065801T2/hu unknown
- 2017-08-03 AU AU2017306657A patent/AU2017306657B2/en active Active
- 2017-08-03 ES ES17790843T patent/ES2971123T3/es active Active
- 2017-08-03 KR KR1020197005510A patent/KR102491566B1/ko active Active
- 2017-08-03 LT LTEPPCT/IB2017/001113T patent/LT3494229T/lt unknown
- 2017-08-03 PL PL17790843.1T patent/PL3494229T3/pl unknown
- 2017-08-03 DK DK17790843.1T patent/DK3494229T3/da active
- 2017-08-03 SM SM20240091T patent/SMT202400091T1/it unknown
- 2017-08-03 WO PCT/IB2017/001113 patent/WO2018025085A2/en active Application Filing
-
2019
- 2019-02-01 PH PH12019500239A patent/PH12019500239A1/en unknown
-
2022
- 2022-06-09 JP JP2022093742A patent/JP2022113773A/ja active Pending
- 2022-06-27 US US17/809,043 patent/US20230065720A1/en active Pending
-
2023
- 2023-03-08 AU AU2023201431A patent/AU2023201431A1/en active Pending
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