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DK3129487T3 - Forbedrede nukleinsyrekonstruktioner til eukaryot genekspression - Google Patents

Forbedrede nukleinsyrekonstruktioner til eukaryot genekspression Download PDF

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Publication number
DK3129487T3
DK3129487T3 DK15776901.9T DK15776901T DK3129487T3 DK 3129487 T3 DK3129487 T3 DK 3129487T3 DK 15776901 T DK15776901 T DK 15776901T DK 3129487 T3 DK3129487 T3 DK 3129487T3
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DK
Denmark
Prior art keywords
genepression
eukaryot
nucleic acid
improved nucleic
acid constructions
Prior art date
Application number
DK15776901.9T
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English (en)
Inventor
Jeremy Minshull
Mark Welch
Sridhar Govindrajan
Kate Caves
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Dna Twopointo Inc
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First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=54264598&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DK3129487(T3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Dna Twopointo Inc filed Critical Dna Twopointo Inc
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Publication of DK3129487T3 publication Critical patent/DK3129487T3/da

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/7088—Compounds having three or more nucleosides or nucleotides
    • A61K31/7105—Natural ribonucleic acids, i.e. containing only riboses attached to adenine, guanine, cytosine or uracil and having 3'-5' phosphodiester links
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43563—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from insects
    • C07K14/43586—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from insects from silkworms
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/463—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from amphibians
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/32—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against translation products of oncogenes
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    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2/00—Peptides of undefined number of amino acids; Derivatives thereof
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
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    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/24—Immunoglobulins specific features characterized by taxonomic origin containing regions, domains or residues from different species, e.g. chimeric, humanized or veneered
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/09—Fusion polypeptide containing a localisation/targetting motif containing a nuclear localisation signal
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/80—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor
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    • C12N2800/00—Nucleic acids vectors
    • C12N2800/90—Vectors containing a transposable element
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/40—Vector systems having a special element relevant for transcription being an insulator
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/60—Vector systems having a special element relevant for transcription from viruses

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  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
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  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
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  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
DK15776901.9T 2014-04-09 2015-04-09 Forbedrede nukleinsyrekonstruktioner til eukaryot genekspression DK3129487T3 (da)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US201461977474P 2014-04-09 2014-04-09
US201462003397P 2014-05-27 2014-05-27
US201462046875P 2014-09-05 2014-09-05
US201462046705P 2014-09-05 2014-09-05
US201462069656P 2014-10-28 2014-10-28
US201562120522P 2015-02-25 2015-02-25
PCT/US2015/025209 WO2015157579A2 (en) 2014-04-09 2015-04-09 Enhanced nucleic acid constructs for eukaryotic gene expression

Publications (1)

Publication Number Publication Date
DK3129487T3 true DK3129487T3 (da) 2020-11-30

Family

ID=54264598

Family Applications (1)

Application Number Title Priority Date Filing Date
DK15776901.9T DK3129487T3 (da) 2014-04-09 2015-04-09 Forbedrede nukleinsyrekonstruktioner til eukaryot genekspression

Country Status (8)

Country Link
US (5) US9428767B2 (da)
EP (2) EP3670660A1 (da)
CA (1) CA2944887A1 (da)
DK (1) DK3129487T3 (da)
ES (1) ES2835673T3 (da)
HU (1) HUE052552T2 (da)
LT (1) LT3129487T (da)
WO (1) WO2015157579A2 (da)

Families Citing this family (47)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9428767B2 (en) 2014-04-09 2016-08-30 Dna2.0, Inc. Enhanced nucleic acid constructs for eukaryotic gene expression
US10927384B2 (en) 2014-04-09 2021-02-23 Dna Twopointo Inc. DNA vectors, transposons and transposases for eukaryotic genome modification
DK3359671T3 (da) 2015-10-08 2021-09-20 Dna Twopointo Inc Dna-vektorer, transposoner og transposaser til eukaryot genommodifikation
WO2017075237A2 (en) * 2015-10-27 2017-05-04 Celltheon Corporation Chimeric post-transcriptional regulatory element
JP6956416B2 (ja) * 2015-12-14 2021-11-02 ゲノムフロンティア セラピューティクス，インコーポレイテッド トランスポゾン系、それを含むキット及びそれらの使用
US11261462B2 (en) 2016-01-27 2022-03-01 Just-Evotec Biologics, Inc. Inducible expression from transposon-based vectors and uses
US11098310B2 (en) 2016-01-27 2021-08-24 Just-Evotec Biologics, Inc. Expression from transposon-based vectors and uses
WO2017132376A1 (en) 2016-01-27 2017-08-03 Just Biotherapeutics, Inc. Hybrid promoter and uses thereof
US11111505B2 (en) 2016-03-19 2021-09-07 Exuma Biotech, Corp. Methods and compositions for transducing lymphocytes and regulating the activity thereof
US11325948B2 (en) 2016-03-19 2022-05-10 Exuma Biotech Corp. Methods and compositions for genetically modifying lymphocytes to express polypeptides comprising the intracellular domain of MPL
US20190300875A1 (en) * 2016-07-11 2019-10-03 The Regents Of The University Of California Bic inhibitor of cry-cry and cry-cib oligomerization/clustering
KR102757155B1 (ko) * 2016-09-30 2025-01-21 포세이다 테라퓨틱스, 인크. 변형된 줄기세포 기억 t 세포, 이의 제조 방법 및 사용 방법
US20190119636A1 (en) 2017-10-23 2019-04-25 Poseida Therapeutics, Inc. Modified stem cell memory t cells, methods of making and methods of using same
EP3555273B1 (en) 2016-12-16 2024-05-22 B-Mogen Biotechnologies, Inc. ENHANCED hAT FAMILY TRANSPOSON-MEDIATED GENE TRANSFER AND ASSOCIATED COMPOSITIONS, SYSTEMS, AND METHODS
US11278570B2 (en) 2016-12-16 2022-03-22 B-Mogen Biotechnologies, Inc. Enhanced hAT family transposon-mediated gene transfer and associated compositions, systems, and methods
MX2019011040A (es) * 2017-03-17 2020-01-20 Adverum Biotechnologies Inc Composiciones y metodos para potenciar la expresion genica.
US11713468B2 (en) 2017-08-02 2023-08-01 Dna Twopointo Inc. DNA vectors and elements for sustained gene expression in eukaryotic cells
US10329543B2 (en) 2017-10-23 2019-06-25 Poseida Therapeutics, Inc. Modified stem cell memory T cells, methods of making and methods of using same
CN109988759A (zh) * 2017-12-29 2019-07-09 上海细胞治疗研究院 一种在t细胞中具有高转录活性的嵌合启动子
EP3794128A1 (en) * 2018-05-18 2021-03-24 Sorbonne Universite Molecular tools and methods for transgene integration and their transposition dependent expression
WO2019246486A2 (en) 2018-06-21 2019-12-26 B-Mogen Biotechnologies, Inc. ENHANCED hAT FAMILY TRANSPOSON-MEDIATED GENE TRANSFER AND ASSOCIATED COMPOSITIONS, SYSTEMS, AND METHODS
WO2020047165A1 (en) * 2018-08-29 2020-03-05 Seattle Children's Hospital (dba Seattle Children's Research Institute) Self-inactivating transposase plasmids and uses thereof
KR20210101270A (ko) * 2018-12-10 2021-08-18 암젠 인크 돌연변이 피기백 트랜스포사제
WO2020132595A1 (en) * 2018-12-21 2020-06-25 The Regents Of The University Of California Il-10-containing vaccines and uses thereof
AU2019429609A1 (en) 2019-02-13 2021-07-22 Probiogen Ag Transposase with enhanced insertion site selection properties
CN113439118A (zh) 2019-02-15 2021-09-24 济世易为生物有限公司 自动化生物制造系统、设备和方法
JP2022523564A (ja) 2019-03-04 2022-04-25 アイオーカレンツ， インコーポレイテッド 機械学習を使用するデータ圧縮および通信
CN118667845A (zh) * 2019-04-08 2024-09-20 Dna2.0股份有限公司 使用来自青鳉属的转座酶将核酸构建体整合入真核细胞
BR112021020144A2 (pt) * 2019-04-08 2021-12-14 Dna Twopointo Inc Transposição de construções de ácidos nucleicos em genomas eucarióticos com uma transposase de amyelois
CN114127083B (zh) 2019-05-13 2024-11-15 Dna2.0股份有限公司 使用人工微rna修饰哺乳动物细胞以改变其特性及其产品的组成
JP2022546101A (ja) * 2019-09-01 2022-11-02 エクスマ バイオテック コーポレイション リンパ球の改変および送達のための方法および組成物
JP7483907B2 (ja) * 2020-02-19 2024-05-15 ウーシー バイオロジクス アイルランド リミテッド 強化された発現系及びその使用方法
CN115698268A (zh) 2020-05-04 2023-02-03 萨利欧基因治疗公司 基于转座的疗法
AU2020458769A1 (en) * 2020-07-17 2023-02-09 Probiogen Ag Hyperactive transposons and transposases
AU2021368557A1 (en) * 2020-10-27 2023-06-08 Adoc Ssf, Llc Compositions and methods for the treatment of cancer using next generation engineered t cell therapy
CN112481289B (zh) * 2020-12-04 2023-06-27 苏州科锐迈德生物医药科技有限公司 一种转录环状rna的重组核酸分子及其在蛋白表达中的应用
WO2022159635A1 (en) * 2021-01-20 2022-07-28 The Texas A&M University System Systems and methods for evaluation of structure and property of polynucleotides
CN117043194A (zh) 2021-01-29 2023-11-10 默沙东有限责任公司 程序性死亡受体1（pd-1）抗体的组合物以及获得所述组合物的方法
EP4308712A4 (en) * 2021-03-15 2025-03-12 Donald Danforth Plant Science Center TARGETED INSERTION BY TRANSPOSITION
CN113186221A (zh) * 2021-05-24 2021-07-30 广西大学 水牛源lif的制备及其在水牛精原干细胞体外培养的应用
WO2023287707A1 (en) 2021-07-15 2023-01-19 Just-Evotec Biologics, Inc. Bidirectional tangential flow filtration (tff) perfusion system
CN113945897B (zh) * 2021-09-03 2024-08-02 西安电子科技大学 一种高超声速平台杂波抑制和运动目标定位方法及装置
IL312573A (en) * 2021-11-04 2024-07-01 Saliogen Therapeutics Inc Mobile elements and chimeric constructs thereof
JP2025505576A (ja) * 2022-02-08 2025-02-28 アイオワ・ステイト・ユニバーシティ・リサーチ・ファウンデイション・インコーポレイテッド ｍＲＮＡの発現及び送達システム
WO2024228717A2 (en) 2022-09-07 2024-11-07 Quantitative Biosciences, Inc. Fentanyl-specific single variable-domain antibodies and use in a continuous agglutination assay
WO2024068995A1 (en) 2022-09-30 2024-04-04 Boehringer Ingelheim International Gmbh Novel transposase system
WO2024155890A2 (en) * 2023-01-19 2024-07-25 Dna Twopointo, Inc. Dna construct configurations for production of protein biologics

Family Cites Families (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5681702A (en) 1994-08-30 1997-10-28 Chiron Corporation Reduction of nonspecific hybridization by using novel base-pairing schemes
ZA973367B (en) 1996-04-19 1997-11-18 Innogenetics Nv Method for typing and detecting HBV.
US6136597A (en) 1997-09-18 2000-10-24 The Salk Institute For Biological Studies RNA export element
US6897066B1 (en) * 1997-09-26 2005-05-24 Athersys, Inc. Compositions and methods for non-targeted activation of endogenous genes
US20030150007A1 (en) 2000-03-21 2003-08-07 Charalambos Savakis Method of generating transgenic organisms using transposons
JP4762478B2 (ja) 2000-07-03 2011-08-31 キャタレント ファーマ ソリューションズ リミテッド ライアビリティ カンパニー 発現ベクター
US8005620B2 (en) 2003-08-01 2011-08-23 Dna Twopointo Inc. Systems and methods for biopolymer engineering
TW200613554A (en) * 2004-06-17 2006-05-01 Wyeth Corp Plasmid having three complete transcriptional units and immunogenic compositions for inducing an immune response to HIV
CA2578908C (en) * 2005-03-17 2020-05-26 Yves Durocher Expression vectors for enhanced transient gene expression and mammalian cells expressing them
US20100154070A1 (en) 2005-05-14 2010-06-17 Tian Xu PiggyBac as a Tool for Genetic Manipulation and Analysis in Vertebrates
TWI300441B (en) 2005-09-21 2008-09-01 Univ Chung Yuan Christian A polynucleotide with ires activity
WO2007100821A2 (en) 2006-02-28 2007-09-07 University Of Notre Dame Piggybac constructs in vertebrates
ES2547015T3 (es) * 2007-03-07 2015-09-30 Aarhus Universitet Modelo de aterosclerosis en cerdos
US20110130444A1 (en) * 2007-05-04 2011-06-02 Stefan Moisyadi Methods and compositions for targeted delivery of gene therapeutic vectors
US20090042297A1 (en) * 2007-06-01 2009-02-12 George Jr Alfred L Piggybac transposon-based vectors and methods of nucleic acid integration
GB0903207D0 (en) * 2009-02-25 2009-04-08 Ucb Pharma Sa Method for expressing multimeric proteins
EP3184632B1 (en) 2009-02-26 2024-04-03 Poseida Therapeutics, Inc. Hyperactive piggybac transposases
US9790489B2 (en) * 2009-10-26 2017-10-17 Chang Gung University Method for performing genetic modification under a drug-free environment and components thereof
ES2716577T3 (es) * 2012-07-31 2019-06-13 Agex Therapeutics Inc Células HLA-G modificadas y métodos
US9428767B2 (en) 2014-04-09 2016-08-30 Dna2.0, Inc. Enhanced nucleic acid constructs for eukaryotic gene expression

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US9428767B2 (en) 2016-08-30
LT3129487T (lt) 2020-12-28
CA2944887A1 (en) 2015-10-15
EP3129487B1 (en) 2020-10-07
US20150291975A1 (en) 2015-10-15
EP3129487A4 (en) 2017-09-13
US9534234B2 (en) 2017-01-03
EP3670660A1 (en) 2020-06-24
ES2835673T3 (es) 2021-06-22
US20160340691A1 (en) 2016-11-24
US20150291977A1 (en) 2015-10-15
US20160333328A1 (en) 2016-11-17
EP3129487A2 (en) 2017-02-15
US9580697B2 (en) 2017-02-28
WO2015157579A2 (en) 2015-10-15
WO2015157579A3 (en) 2015-12-17
HUE052552T2 (hu) 2021-05-28
US20150291976A1 (en) 2015-10-15
US9574209B2 (en) 2017-02-21

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