DK3072962T3 - Fremgangsmåde til fremstilling af fytasevariant med forbedret termostabilitet og en fytasevariant og anvendelse deraf - Google Patents
Fremgangsmåde til fremstilling af fytasevariant med forbedret termostabilitet og en fytasevariant og anvendelse deraf Download PDFInfo
- Publication number
- DK3072962T3 DK3072962T3 DK13897309.4T DK13897309T DK3072962T3 DK 3072962 T3 DK3072962 T3 DK 3072962T3 DK 13897309 T DK13897309 T DK 13897309T DK 3072962 T3 DK3072962 T3 DK 3072962T3
- Authority
- DK
- Denmark
- Prior art keywords
- phytase
- variants
- activity
- seq
- phytase variants
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/189—Enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y301/00—Hydrolases acting on ester bonds (3.1)
- C12Y301/03—Phosphoric monoester hydrolases (3.1.3)
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Polymers & Plastics (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Food Science & Technology (AREA)
- Animal Husbandry (AREA)
- Botany (AREA)
- Mycology (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Claims (10)
1. Fremgangsmåde til fremstilling af fytasevarianter med en forbedret termostabilitet, inklusive trinnene med indføring af seks proliner på site S80, S151, T161, N176, S187 og A380 af fytasen med aminosyresekvensen, der er anført i SEQ ID NO:2, hvor fytasevarian-terne er mindst 70 % homologe med aminosyresekvensen, der er anført i SEQ ID NO:2.
2. Fytasevarianter med en forbedret termostabilitet med følgende kendetegn: med mindst 70 % homologi med aminosyresekvensen, der er anført i SEQ ID NO:2.; og seks proliner er indført på site S80, S151, T161, N176, S187 og A380 af aminosyresekvensen, der er anført i SEQ ID NO:2.
3. Polynukleotid, der koder for fytasevarianterne med en forbedret termostabilitet ifølge krav 2.
4. Nukleotidkonstruktion omfattende polynukleotidet ifølge krav 3 og en regulerende sekvens, der eventuelt er forbundet til et eller flere af polynukleotiderne for at styre ekspressionen i ekspressionsværten.
5. Rekombinant ekspressionsvektor omfattende nukleotidkonstruktionen ifølge krav 4.
6. Rekombinant værtscelle omfattende den rekombinante ekspressionsvektor ifølge krav 5.
7. Rekombinant værtscelle ifølge krav 6, som er kendetegnet ved, at værtscellen er en bakteriecelle eller svampecelle.
8. Fremgangsmåde til fremstilling af fytasevarianter ifølge krav 2 med den forbedrede termostabilitet omfattende trinnene med (a) dyrkning af den rekombinante værtscelle ifølge krav 6 til 7 for at fremstille supernatan-ten indeholdende fytasevarianterne og (b) genfinding af fytasen.
9. Anvendelse af fytasevarianterne med en forbedret termostabilitet ifølge krav 2 som foderadditiv.
10. Anvendelse af den rekombinante værtscelle ifølge krav 6 i fremstilling af foderadditiver.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/CN2013/086943 WO2015070372A1 (zh) | 2013-11-12 | 2013-11-12 | 一种产生热稳定性改良的植酸酶变体的方法、以及植酸酶变体及其应用 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK3072962T3 true DK3072962T3 (da) | 2019-03-18 |
Family
ID=53056598
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK13897309.4T DK3072962T3 (da) | 2013-11-12 | 2013-11-12 | Fremgangsmåde til fremstilling af fytasevariant med forbedret termostabilitet og en fytasevariant og anvendelse deraf |
Country Status (4)
| Country | Link |
|---|---|
| US (1) | US9765313B2 (da) |
| EP (1) | EP3072962B1 (da) |
| DK (1) | DK3072962T3 (da) |
| WO (1) | WO2015070372A1 (da) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107236717B (zh) * | 2016-03-28 | 2021-03-30 | 青岛蔚蓝生物集团有限公司 | 植酸酶突变体 |
| CN108603181B (zh) * | 2016-06-30 | 2022-03-08 | 福尼亚生物处理股份有限公司 | 植酸酶及其用途 |
| WO2018130212A2 (zh) * | 2017-01-15 | 2018-07-19 | 中国农业科学院饲料研究所 | 胃蛋白抗性改良和催化效率提高的植酸酶YkAPPA突变体 |
| GB2567010A (en) | 2017-10-02 | 2019-04-03 | Univ Strathclyde | Apparatus for the rehabilitation, assistance and/or augmentation of arm strength in a user |
| US20210207112A1 (en) | 2018-05-30 | 2021-07-08 | Nanjing Bestzyme Bio-Engineering Co., Ltd. | Phytase mutant |
| CN114807093B (zh) * | 2022-06-22 | 2022-09-27 | 中国农业科学院北京畜牧兽医研究所 | 一种通过c端添加融合肽段提高木聚糖酶和植酸酶热稳定性的方法 |
Family Cites Families (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7078035B2 (en) * | 1997-08-13 | 2006-07-18 | Diversa Corporation | Phytases, nucleic acids encoding them and methods for making and using them |
| CA2391739C (en) * | 1999-11-18 | 2013-04-09 | Cornell Research Foundation, Inc. | Site-directed mutagenesis of escherichia coli phytase |
| ATE490309T1 (de) * | 2002-08-12 | 2010-12-15 | Danisco Us Inc | Mutante e. coli-appa-phytaseenzyme und natürliche varianten davon, solche phytaseenzyme codierende nukleinsäuren, diese enthaltende vektoren und wirtszellen und verfahren zur herstellung und verwendung davon |
| CN1706941A (zh) * | 2004-06-10 | 2005-12-14 | 安徽固源生物工程有限责任公司 | 高比活植酸酶的热稳定性改良及其高效表达 |
| WO2008017066A2 (en) * | 2006-08-03 | 2008-02-07 | Cornell Research Foundation, Inc. | Phytases with improved thermal stability |
| ES2548776T3 (es) | 2006-09-21 | 2015-10-20 | Basf Enzymes Llc | Fitasas, ácidos nucleicos que las codifican y métodos para su producción y uso |
| DE102006053059A1 (de) * | 2006-11-10 | 2008-05-15 | Ab Enzymes Gmbh | Polypeptid mit Phytaseaktivität und erhöhter Temperaturstabilität der Enzymaktivität sowie dieses codierende Nukleotidsequenz |
| WO2009110933A2 (en) | 2007-12-03 | 2009-09-11 | Syngenta Participations Ag | Engineering enzymatically susceptible phytases |
| BRPI0823236A2 (pt) * | 2008-11-14 | 2015-06-16 | Fujian Fuda Biotech Co Ltd | Fitase termotolerante de escherichia coli não-k12 e sua produção. |
| FR2947563B1 (fr) * | 2009-07-01 | 2015-08-21 | Biomethodes | Variants ameliores d'une phytase |
| CN102002487B (zh) * | 2010-11-03 | 2014-04-30 | 广东溢多利生物科技股份有限公司 | 一种优化改良的耐高温植酸酶phyth及其基因和应用 |
| CN102392002B (zh) * | 2011-11-03 | 2013-06-19 | 青岛蔚蓝生物集团有限公司 | 一种改进的大肠杆菌植酸酶htp6m及其基因和应用 |
-
2013
- 2013-11-12 DK DK13897309.4T patent/DK3072962T3/da active
- 2013-11-12 WO PCT/CN2013/086943 patent/WO2015070372A1/zh active Application Filing
- 2013-11-12 US US15/112,198 patent/US9765313B2/en active Active
- 2013-11-12 EP EP13897309.4A patent/EP3072962B1/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| EP3072962A4 (en) | 2017-06-14 |
| EP3072962A1 (en) | 2016-09-28 |
| US9765313B2 (en) | 2017-09-19 |
| EP3072962B1 (en) | 2018-12-26 |
| WO2015070372A1 (zh) | 2015-05-21 |
| US20170022486A1 (en) | 2017-01-26 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| DK3072962T3 (da) | Fremgangsmåde til fremstilling af fytasevariant med forbedret termostabilitet og en fytasevariant og anvendelse deraf | |
| CN101426907B (zh) | 一种植酸酶的克隆和表达 | |
| JP2007061105A (ja) | 酵素混合物 | |
| Mi et al. | Molecular cloning and characterization of a novel α-galactosidase gene from Penicillium sp. F63 CGMCC 1669 and expression in Pichia pastoris | |
| CN101631855B (zh) | 具有肌醇六磷酸酶活性且该酶活性的温度稳定性增强的多肽及编码所述多肽的核苷酸序列 | |
| KR20110092511A (ko) | 셀룰로시마이크로비움 펀케이 ｈｙ－１３ 균주로부터 생산되는 신규한 자일라나아제 | |
| CN101080491B (zh) | 具有植酸酶活性的多肽和编码多肽的核苷酸序列 | |
| KR20150001160A (ko) | 흑염소 반추위 미생물 유래의 섬유소분해효소 cel45-KG80 유전자 및 이의 용도 | |
| CN101063113B (zh) | 一种新的植酸酶的克隆和表达 | |
| CN102978182A (zh) | 脂肪酶突变体 | |
| CN102559632A (zh) | 一种在酸性范围内催化活性提高的优化改良的大肠杆菌植酸酶appa-m及其基因和应用 | |
| CN113025593B (zh) | 一种亲本植酸酶变体 | |
| Zou et al. | Design and expression of a synthetic phyC gene encoding the neutral phytase in Pichia pastoris | |
| CN101260390B (zh) | 高比活植酸酶基因及其应用 | |
| CN109694858B (zh) | 一种植酸酶突变体 | |
| CN101368175A (zh) | 一种新的植酸酶及其编码基因以及包含该酶的细胞和饲料添加剂 | |
| KR101437491B1 (ko) | 흑염소 반추위 미생물 유래의 자일라나아제 활성 효소 cel10-CBM6-KG61 유전자 및 이의 용도 | |
| KR101479687B1 (ko) | 흑염소 반추위 미생물 유래의 셀룰라아제 유전자 ｃｅｌ－ＫＧ５２ 및 이의 용도 | |
| KR101479708B1 (ko) | 흑염소 반추위 미생물 유래의 엔도―1，4―베타―자일라나아제 ｃｅｌ１０－ＫＧ０４ 유전자 및 이의 용도 | |
| KR101475838B1 (ko) | 신규 자일라나제, 이를 코딩하는 유전자 및 이의 용도 | |
| KR101479698B1 (ko) | 흑염소 반추위 미생물 유래의 셀룰라아제 유전자 ｃｅｌ－ＫＧ２０ 및 이의 용도 | |
| KR101479696B1 (ko) | 흑염소 반추위 미생물 유래의 엔도글루카나아제 ｃｅｌ－ＫＧ３５ 유전자 및 이의 용도 | |
| KR101444273B1 (ko) | 흑염소 반추위 미생물 유래의 섬유소분해효소 cel5-KG71 유전자 및 이의 용도 | |
| KR101483582B1 (ko) | 흑염소 반추위 미생물 유래의 엔도자일라나아제 ｃｅｌ１０－ＫＧ４２ 유전자 및 이의 용도 | |
| KR20130068006A (ko) | 흑염소 반추위 미생물 유래의 베타 글루코시다아제 ｃｅｌ３－ＫＧ３９ 유전자 및 이의 용도 |