DK2922961T3 - Nukleotidsekvens, der koder for wuschel-relateret homeobox4-(wox4) protein fra corchorus olitorius og corchorus capsularis og fremgangsmåder til anvendelse heraf - Google Patents
Nukleotidsekvens, der koder for wuschel-relateret homeobox4-(wox4) protein fra corchorus olitorius og corchorus capsularis og fremgangsmåder til anvendelse heraf Download PDFInfo
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- DK2922961T3 DK2922961T3 DK14859333.8T DK14859333T DK2922961T3 DK 2922961 T3 DK2922961 T3 DK 2922961T3 DK 14859333 T DK14859333 T DK 14859333T DK 2922961 T3 DK2922961 T3 DK 2922961T3
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- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
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- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
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- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
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Claims (14)
1. Isoleret polynukleotid, der koder for et WUSCHEL-relateret homeobox4-polypeptid afledt af planten C. olitorius eller C. capsularis, der omfatter et nukleinsyremolekyle udvalgt fra gruppen bestående af: a) et nukleinsyremolekyle, der omfatter en nukleotidsekvens ifølge SEQ ID NO: 2 eller SEQ ID NO: 5, eller et komplement deraf; og b) et nukleinsyremolekyle, der omfatter en nukleotidsekvens med mindst 95 % sekvensidentitet med nukleotidet ifølge SEQ ID NO: 2 eller SEQ ID NO: 5, eller et komplement deraf.
2. Kodende isoleret polynukleotid ifølge krav 1, hvor planten af C. olitorius er varietet O- 4.
3. Isoleret polynukleotid ifølge krav 1, hvor planten af C. capsularis er varietet CVL- 1.
4. Isoleret WUSCHEL-relateret homeobox4-polypeptid, der omfatter en aminosyresekvens udvalgt fra gruppen bestående af: (a) en aminosyresekvens ifølge SEQ ID: NO: 3 eller SEQ ID NO: 6 eller et biologisk aktivt fragment deraf; (b) en aminosyresekvens, der omfatter en aminosyresekvens med mindst 95 % sekvensidentitet med aminosyresekvensen ifølge SEQ ID NO: 3 eller 6; polypeptidet omfatter en funktion udvalgt fra gruppen bestående af katalysering af initieringen, dannelsen, forbedringen og variationen for derved at ændre sammensætningen af floemfiber i planten af C. olitorius eller C. capularis.
5. Isoleret polynukleotid ifølge krav 4, hvor planten af C. olitorius er varietet 0-4.
6. Isoleret polynukleotid ifølge krav 4, hvor planten af C. capsularis er varietet CVL-1.
7. Rekombinant genkonstrukt, der omfatter det isolerede polynukleotid ifølge et hvilket som helst af de foregående krav 1 til 3, hvor polynukleotidet kan udtrykkes i en værtscelle for at frembringe en homolog af et WUSCHEL-relateret homeobox4-polypeptid fra planterne af C. olitorius og C. capsularis.
8. Rekombinant genkonstrukt ifølge krav 7, der endvidere omfatter et promoterområde, der er operativt bundet til et nukleinsyremolekyle ifølge a) eller b), hvor promoteren forbedrer transskription eller ekspression af nukleinsyremolekylet.
9. Transformant, der omfatter et rekombinant genkonstrukt ifølge krav 7, der er i stand til at udtrykke et polynukleotid, hvor transformanten frembringer en homolog af WUSCHEL-relateret homeobox4-polypeptid.
10. Fremgangsmåde til fremstilling af en plante eller transgen plante med øget eller forbedret fiberudbytte i forhold til kontrolplanter, hvilken fremgangsmåde omfatter: a) indføring i en planteceller af et rekombinant genkonstrukt, der omfatter et polynukleotid ifølge krav 7; og b) dyrkning af plantecellen under forhold, der fremmer plantevækst og -udvikling og c) ekspression af et polypeptid udvalgt fra gruppen bestående af: i) et polypeptid, der omfatter en aminosyresekvens ifølge SEQ ID: NO: 3 eller SEQ ID NO: 6, eller et biologisk aktivt fragment deraf; og ii) et polypeptid med mindst 95 % sekvensidentitet med aminosyresekvensen ifølge SEQ ID NO: 3 eller SEQ ID NO: 6.
11. Isoleret polynukleotid ifølge krav 1, hvor polynukleotidet har: mindst 98 % sekvensidentitet med nukleotidsekvensen ifølge SEQ ID NO 2 eller SEQ ID NO: 5.
12. Isoleret polynukleotid ifølge krav 4, hvor polypeptidet har: mindst 98 % sekvensidentitet med aminosyresekvensen ifølge SEQ ID: NO: 3 eller SEQ ID NO: 6.
13. Frø fra en transformant ifølge krav 9, hvor frøet omfatter det isolerede polynukleotid ifølge et hvilket som helst af kravene 1-3 og 11.
14. Fremgangsmåde til induktion, initiering, forbedring eller styrkelse af plantevækst, plantehøjde, udbytte af plantefiber- og frø for en juteplante, der omfatter inkorporering i en juteplante af det rekombinante genkonstrukt ifølge krav 8.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201361907617P | 2013-11-22 | 2013-11-22 | |
| PCT/US2014/066599 WO2015077447A2 (en) | 2013-11-22 | 2014-11-20 | Nucleotide sequence encoding wuschel-related homeobox4 (wox4) protein from corchorus olitorius and corchorus capsularis and methods of use for same |
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| Publication Number | Publication Date |
|---|---|
| DK2922961T3 true DK2922961T3 (da) | 2018-06-14 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK14859333.8T DK2922961T3 (da) | 2013-11-22 | 2014-11-20 | Nukleotidsekvens, der koder for wuschel-relateret homeobox4-(wox4) protein fra corchorus olitorius og corchorus capsularis og fremgangsmåder til anvendelse heraf |
Country Status (17)
| Country | Link |
|---|---|
| US (1) | US10125371B2 (da) |
| EP (1) | EP2922961B1 (da) |
| JP (1) | JP6836904B2 (da) |
| KR (1) | KR102069045B1 (da) |
| CN (1) | CN105164262A (da) |
| AU (1) | AU2014352999B2 (da) |
| BR (1) | BR112015011990B1 (da) |
| DK (1) | DK2922961T3 (da) |
| ES (1) | ES2672349T3 (da) |
| HK (1) | HK1218431A1 (da) |
| HU (1) | HUE039294T2 (da) |
| MY (1) | MY186538A (da) |
| PL (1) | PL2922961T3 (da) |
| PT (1) | PT2922961T (da) |
| TR (1) | TR201807621T4 (da) |
| WO (1) | WO2015077447A2 (da) |
| ZA (1) | ZA201503657B (da) |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| EP2922961B1 (en) * | 2013-11-22 | 2018-05-16 | Bangladesh Jute Research Institute | Nucleotide sequence encoding wuschel-related homeobox4 (wox4) protein from corchorus olitorius and corchorus capsularis and methods of use for same |
| CN108491692B (zh) * | 2018-03-09 | 2023-07-21 | 中国科学院生态环境研究中心 | 一种构建抗生素抗性基因数据库的方法 |
| CN109485708B (zh) * | 2018-11-09 | 2023-12-05 | 中国中医科学院中药研究所 | 用于检测人参维管束干细胞的特异性基因PgWOX4及其检测方法与应用 |
| CN112522296B (zh) * | 2019-08-30 | 2022-09-30 | 中国科学技术大学 | Wuschel基因及其编码蛋白在植物抗病毒中的应用 |
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| DE60034929T2 (de) * | 1999-10-01 | 2008-01-24 | E.I. Du Pont De Nemours And Co., Wilmington | Wuschel (WUS)-Proteine |
| US20030082813A1 (en) * | 2001-10-29 | 2003-05-01 | The Rockefeller University | Promotion of somatic embryogenesis in plants by wuschel gene expression |
| KR20060030577A (ko) * | 2004-10-06 | 2006-04-11 | 삼성전자주식회사 | 박막 트랜지스터 표시판 |
| AU2007252854A1 (en) * | 2006-05-23 | 2007-11-29 | Ramot At Tel-Aviv University Ltd. | Compositions for silencing the expression of gibberellin 2-oxidase and uses thereof |
| BRPI1007465A2 (pt) * | 2009-01-28 | 2015-11-24 | Basf Plant Science Co Gmbh | método para realçar traços relacionados com o rendimento em plantas em relação às plantas de controle, para produzir uma planta transgênica tendo rendimento aumentado, molécula de ácido nucleico isolada, polipeptídio isolado, construçao, uso de uma construção e de um ácido nucleico, célula de planta, e, produtos |
| ES2645742T3 (es) * | 2011-04-29 | 2017-12-07 | Bangladesh Jute Research Institute | Enzimas que codifican polinucleótidos de la senda biosintética de lignina del yute |
| EP2922961B1 (en) | 2013-11-22 | 2018-05-16 | Bangladesh Jute Research Institute | Nucleotide sequence encoding wuschel-related homeobox4 (wox4) protein from corchorus olitorius and corchorus capsularis and methods of use for same |
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- 2014-11-20 EP EP14859333.8A patent/EP2922961B1/en active Active
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- 2014-11-20 US US14/781,169 patent/US10125371B2/en active Active
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Also Published As
| Publication number | Publication date |
|---|---|
| ZA201503657B (en) | 2017-01-25 |
| TR201807621T4 (tr) | 2018-06-21 |
| US10125371B2 (en) | 2018-11-13 |
| WO2015077447A3 (en) | 2015-08-13 |
| BR112015011990A2 (pt) | 2019-10-15 |
| KR20160085208A (ko) | 2016-07-15 |
| ES2672349T3 (es) | 2018-06-14 |
| CN105164262A (zh) | 2015-12-16 |
| EP2922961A4 (en) | 2015-12-30 |
| US20160369288A1 (en) | 2016-12-22 |
| WO2015077447A2 (en) | 2015-05-28 |
| KR102069045B1 (ko) | 2020-01-22 |
| EP2922961A2 (en) | 2015-09-30 |
| JP6836904B2 (ja) | 2021-03-03 |
| HUE039294T2 (hu) | 2018-12-28 |
| EP2922961B1 (en) | 2018-05-16 |
| MY186538A (en) | 2021-07-26 |
| JP2017500888A (ja) | 2017-01-12 |
| PT2922961T (pt) | 2018-06-06 |
| HK1218431A1 (zh) | 2017-02-17 |
| AU2014352999B2 (en) | 2020-12-24 |
| BR112015011990B1 (pt) | 2022-10-11 |
| PL2922961T3 (pl) | 2018-09-28 |
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