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DK2646558T3 - Promotorer til ekspression af gener i en svampecelle - Google Patents

Promotorer til ekspression af gener i en svampecelle Download PDF

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Publication number
DK2646558T3
DK2646558T3 DK11796871.9T DK11796871T DK2646558T3 DK 2646558 T3 DK2646558 T3 DK 2646558T3 DK 11796871 T DK11796871 T DK 11796871T DK 2646558 T3 DK2646558 T3 DK 2646558T3
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DK
Denmark
Prior art keywords
seq
polypeptide
promoter
dna
sequence
Prior art date
Application number
DK11796871.9T
Other languages
English (en)
Inventor
Michael Catlett
Debbie Yaver
Original Assignee
Novozymes Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes Inc filed Critical Novozymes Inc
Application granted granted Critical
Publication of DK2646558T3 publication Critical patent/DK2646558T3/da

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Mycology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Claims (7)

1. Isoleret promotor valgt fra gruppen bestående af (i) en promotor bestående af en nukleotidsekvens med i det mindste 70% sekvensidentitet med SEQ ID NR: 1, SEQ ID NR: 2, SEQ ID NR: 3, eller SEQ ID NR: 4; (ii) en promotor bestående af en nukleotidsekvens, som hybridiserer under i det mindste middel stringensbetingelser med SEQ ID NR: 1, SEQ ID NR: 2, SEQ ID NR: 3, eller SEQ ID NR: 4; eller hybridiserer den fuld-længde komplementære sekvens deraf (Hi) en promoter bestående af SEQ ID NR: 1, SEQ ID NR: 2, SEQ ID NR: 3, eller SEQ ID NR: 4.
2. Promoter ifølge krav 1, som består af den polynucleotide sekvens af SEQ ID NR: 1, SEQ ID NR: 2, SEQ ID NR: 3, eller SEQ ID NR: 4.
3. Nukleinsyrekonstrukt omfattende et polynukleotid, der koder for et polypeptid, der er operabelt forbundet til en promoter ifølge ethvert af kravene 1 - 2.
4. Rekombinant værtscelle omfattende nukleinsyrekonstruktet ifølge krav 3.
5. Fremgansmåde til produktion af et polypeptid omfattende: (a) kultivering af en fungal værtscelle i et medium, der er fremmende for produktionen af polypeptidet, hvor den fungale værtscelle omfatter et polynukleotid, der koder for polypeptidet, operabelt forbundet til en promoter, der er valgt fra gruppen bestående af (i) en promotor bestående af en nukleotidsekvens med i det mindste 70% sekvensidentitet med SEQ ID NR: 1, SEQ ID NR: 2, SEQ ID NR: 3, eller SEQ ID NR: 4; (ii) en promotor bestående af en nukleotidsekvens, som hybridiserer under i det mindste middel stringensbetingelser med SEQ ID NR: 1, SEQ ID NR: 2, SEQ ID NR: 3, eller SEQ ID NR: 4; eller med den fuld-længde komplementære sekvens deraf; (iii) en promoter bestående af SEQ ID NR: 1, SEQ ID NR: 2, SEQ ID NR: 3, eller SEQ ID NR: 4. (b) isolering af polypeptidet fra kultiveringsmediet.
6. Fremgangsmåde ifølge krav 5, hvor promoteren består af polynukleotidsekvensen af SEQ ID NR: 1, SEQ ID NR: 2, SEQ ID NR: 3, eller SEQ ID NR: 4.
7. Fremgangsmåde ifølge ethvert af kravene 5-6, hvor polypeptidet er nativt eller fremmed overfor den fungale værtscelle.
DK11796871.9T 2010-11-30 2011-11-30 Promotorer til ekspression af gener i en svampecelle DK2646558T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US41830210P 2010-11-30 2010-11-30
PCT/US2011/062663 WO2012075151A2 (en) 2010-11-30 2011-11-30 Promoters for expressing genes in a fungal cell

Publications (1)

Publication Number Publication Date
DK2646558T3 true DK2646558T3 (da) 2016-10-03

Family

ID=45349295

Family Applications (1)

Application Number Title Priority Date Filing Date
DK11796871.9T DK2646558T3 (da) 2010-11-30 2011-11-30 Promotorer til ekspression af gener i en svampecelle

Country Status (5)

Country Link
US (2) US9359630B2 (da)
EP (1) EP2646558B1 (da)
CN (2) CN103339259B (da)
DK (1) DK2646558T3 (da)
WO (1) WO2012075151A2 (da)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2784318T3 (es) * 2016-09-20 2020-09-24 Clariant Int Ltd Método para una expresión selectiva independiente de la fuente de carbono de secuencias que codifican una proteína en una célula de hongo filamentoso
CN108642075A (zh) * 2018-04-10 2018-10-12 深圳大学 适用于太瑞斯梭孢壳霉的表达载体及其制备方法

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK122686D0 (da) 1986-03-17 1986-03-17 Novo Industri As Fremstilling af proteiner
US5989870A (en) 1986-04-30 1999-11-23 Rohm Enzyme Finland Oy Method for cloning active promoters
US5641670A (en) 1991-11-05 1997-06-24 Transkaryotic Therapies, Inc. Protein production and protein delivery
PT765394E (pt) 1994-06-03 2002-03-28 Novozymes Biotech Inc Lacases myceliophthora purificadas e acidos nucleicos que as codificam
AU2705895A (en) 1994-06-30 1996-01-25 Novo Nordisk Biotech, Inc. Non-toxic, non-toxigenic, non-pathogenic fusarium expression system and promoters and terminators for use therein
DK1616580T3 (da) * 1996-07-05 2008-01-14 Novozymes As Transkriptionsfaktor
JP5043254B2 (ja) 1998-10-26 2012-10-10 ノボザイムス アクティーゼルスカブ 糸状面細胞内の問題のｄｎａライブラリーの作製及びスクリーニング
CN100532561C (zh) 1999-03-22 2009-08-26 诺沃奇梅兹有限公司 新的葡糖淀粉酶及编码它的核酸序列
CN100480391C (zh) * 2002-11-18 2009-04-22 诺维信股份有限公司 用于在真菌细胞中表达基因的启动子变体
AU2005206251A1 (en) 2004-01-21 2005-08-04 Novozymes A/S Production of a monoclonal antibody in a heterokaryon fungus or in a fungal host cell
DK1819818T3 (da) * 2004-11-18 2013-05-06 Genencor Int Aspergillus niger-promoter til ekspression af gener i værtsceller
ES2552635T3 (es) 2004-12-22 2015-12-01 Novozymes A/S Polipéptidos con actividad de glucoamilasa y codificación de polinucleótidos
WO2007045248A1 (en) 2005-10-17 2007-04-26 Novozymes A/S Use of fungal mutants for expression of antibodies
US20110223671A1 (en) 2008-09-30 2011-09-15 Novozymes, Inc. Methods for using positively and negatively selectable genes in a filamentous fungal cell

Also Published As

Publication number Publication date
US9359630B2 (en) 2016-06-07
EP2646558A2 (en) 2013-10-09
WO2012075151A2 (en) 2012-06-07
CN103339259A (zh) 2013-10-02
CN103339259B (zh) 2016-01-13
US20160251666A1 (en) 2016-09-01
US20130316399A1 (en) 2013-11-28
WO2012075151A3 (en) 2012-09-07
CN105420267A (zh) 2016-03-23
EP2646558B1 (en) 2016-07-20

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