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DK2379722T3 - Ekspressionsvektor - Google Patents

Ekspressionsvektor

Info

Publication number
DK2379722T3
DK2379722T3 DK09798882.8T DK09798882T DK2379722T3 DK 2379722 T3 DK2379722 T3 DK 2379722T3 DK 09798882 T DK09798882 T DK 09798882T DK 2379722 T3 DK2379722 T3 DK 2379722T3
Authority
DK
Denmark
Prior art keywords
expression
Prior art date
Application number
DK09798882.8T
Other languages
English (en)
Inventor
Thomas Greiner-Stöffele
Meike Ballschmitter
Marc Struhalla
Rico Czaja
Original Assignee
C-Lecta Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by C-Lecta Gmbh filed Critical C-Lecta Gmbh
Application granted granted Critical
Publication of DK2379722T3 publication Critical patent/DK2379722T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1082—Preparation or screening gene libraries by chromosomal integration of polynucleotide sequences, HR-, site-specific-recombination, transposons, viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/635—Externally inducible repressor mediated regulation of gene expression, e.g. tetR inducible by tetracyline
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/40—Systems of functionally co-operating vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/001—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination
    • C12N2830/002—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination inducible enhancer/promoter combination, e.g. hypoxia, iron, transcription factor

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Virology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
DK09798882.8T 2008-12-16 2009-12-15 Ekspressionsvektor DK2379722T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP08021794 2008-12-16
PCT/EP2009/008977 WO2010075956A1 (de) 2008-12-16 2009-12-15 Expressionsvektor

Publications (1)

Publication Number Publication Date
DK2379722T3 true DK2379722T3 (da) 2017-01-02

Family

ID=40636851

Family Applications (1)

Application Number Title Priority Date Filing Date
DK09798882.8T DK2379722T3 (da) 2008-12-16 2009-12-15 Ekspressionsvektor

Country Status (4)

Country Link
US (2) US20120021950A1 (da)
EP (1) EP2379722B1 (da)
DK (1) DK2379722T3 (da)
WO (1) WO2010075956A1 (da)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20150353940A1 (en) * 2013-08-05 2015-12-10 Absci, Llc Vectors for use in an inducible coexpression system
US20220364104A1 (en) * 2012-08-05 2022-11-17 Absci Llc Vectors for use in an inducible coexpression system
EP2860256B1 (en) * 2013-10-11 2017-03-08 CJ Cheiljedang Corporation Method of producing l-amino acids
US9951392B2 (en) * 2014-03-14 2018-04-24 Northwestern University Substrate replenishment and byproduct removal improve yeast cell-free protein synthesis
CN121203988A (zh) 2014-04-22 2025-12-26 C-乐克塔股份有限公司 酮还原酶
WO2016064742A1 (en) * 2014-10-21 2016-04-28 The Board Of Regents Of The Nevada System Of Higher Education On Behalf Of The University Of Nevada, Las Vegas Methods and compositions for screening molecular function comprising chimeric minimotifs
HK1243730A1 (zh) * 2015-05-13 2018-07-20 Csl贝林基因治疗股份有限公司 慢病毒载体的生物生产
ES2868191T3 (es) 2015-06-12 2021-10-21 C Lecta Gmbh Transaminasas
WO2024137857A1 (en) * 2022-12-22 2024-06-27 Tevard Biosciences, Inc. Conditional expression of a gene of interest by convergent promoters and uses thereof

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6030807A (en) 1996-09-10 2000-02-29 The Rockefeller University Highly regulable promoter for heterologous gene expression
US7005423B1 (en) 1999-07-02 2006-02-28 Devgen Nv Characterization of gene function using double stranded RNA inhibition
GB9827152D0 (en) 1998-07-03 1999-02-03 Devgen Nv Characterisation of gene function using double stranded rna inhibition
US6689573B1 (en) 1999-05-24 2004-02-10 New England Biolabs, Inc. Method for screening restriction endonucleases
US6780405B1 (en) 2000-04-28 2004-08-24 Avant Immunotherapeutics, Inc. Regulated antigen delivery system (RADS)
US6977165B2 (en) 2001-01-18 2005-12-20 Clontech Laboratories, Inc. Sequence specific recombinase-based methods for producing intron containing vectors and compositions for use in practicing the same
CA2369944A1 (en) 2001-01-31 2002-07-31 Nucleonics Inc. Use of post-transcriptional gene silencing for identifying nucleic acid sequences that modulate the function of a cell
US7465456B2 (en) * 2002-04-26 2008-12-16 Medimmune, Llc Multi plasmid system for the production of influenza virus
US20040002690A1 (en) 2002-07-01 2004-01-01 The Procter & Gamble Company Disposable absorbent article having elasticized outer leg cuff
US8501464B2 (en) 2003-04-24 2013-08-06 Ospedale San Raffaele S.R.L. Lentiviral vectors carrying synthetic bi-directional promoters and uses thereof
DE10350474C5 (de) 2003-10-23 2009-09-17 C-Lecta Gmbh Verfahren zur Selektion von Biomolekülen aus Varianten-Bibliotheken von Biomolekülen
SG163558A1 (en) * 2006-12-29 2010-08-30 Osprey Pharmaceuticals Usa Inc Methods of selecting and producing modified toxins, conjugates containing modified toxins and uses thereof

Also Published As

Publication number Publication date
EP2379722B1 (de) 2016-09-07
WO2010075956A1 (de) 2010-07-08
US20160194647A1 (en) 2016-07-07
US20120021950A1 (en) 2012-01-26
US10196645B2 (en) 2019-02-05
EP2379722A1 (de) 2011-10-26

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