DK200301156A - Polynucleotide(s) encoding immunoglobulin molecules - used for efficient prodn. of chimeric human or non-human- or class switched-antibodies - Google Patents
Polynucleotide(s) encoding immunoglobulin molecules - used for efficient prodn. of chimeric human or non-human- or class switched-antibodies Download PDFInfo
- Publication number
- DK200301156A DK200301156A DK200301156A DKPA200301156A DK200301156A DK 200301156 A DK200301156 A DK 200301156A DK 200301156 A DK200301156 A DK 200301156A DK PA200301156 A DKPA200301156 A DK PA200301156A DK 200301156 A DK200301156 A DK 200301156A
- Authority
- DK
- Denmark
- Prior art keywords
- chimeric
- human
- polynucleotide
- molecule
- immunoglobulin
- Prior art date
Links
- 108060003951 Immunoglobulin Proteins 0.000 title abstract description 22
- 102000018358 immunoglobulin Human genes 0.000 title abstract description 22
- 108091033319 polynucleotide Proteins 0.000 title abstract description 6
- 102000040430 polynucleotide Human genes 0.000 title abstract description 6
- 239000002157 polynucleotide Substances 0.000 title abstract description 6
- 239000012634 fragment Substances 0.000 abstract description 8
- 230000028327 secretion Effects 0.000 abstract description 8
- 229920001184 polypeptide Polymers 0.000 abstract description 4
- 108090000765 processed proteins & peptides Proteins 0.000 abstract description 4
- 102000004196 processed proteins & peptides Human genes 0.000 abstract description 4
- 230000035897 transcription Effects 0.000 abstract description 2
- 238000013518 transcription Methods 0.000 abstract description 2
- 108020004705 Codon Proteins 0.000 abstract 1
- 108700039691 Genetic Promoter Regions Proteins 0.000 abstract 1
- 238000000034 method Methods 0.000 description 9
- 108010076504 Protein Sorting Signals Proteins 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004317 Lyases Human genes 0.000 description 1
- 108090000856 Lyases Proteins 0.000 description 1
- 230000001747 exhibiting effect Effects 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 230000009452 underexpressoin Effects 0.000 description 1
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
PATENTKRAV 1. Fremgangsmåde til fremstilling af et immunoglobulinmolekyle med tunge og lette kæder eller fragmenter deraf, som er forbundet således, at det overordnede molekyle udviser ønskede bindings- og genkendelsesegenskaber, idet fremgangsmåden omfatter (a) dyrkning af en transformeret eller transfekteret prokaryot vært under passende betingelser, sådan at tungt kædeimmunoglobulin og let kædeimmunoglobulin eller fragmenter deraf udtrykkes som et resultat af transskription af polynucleotid-sekvenser i værten, hvor polynucleotidsekvenseme koder for mindst én funktionelt virkende region af en variabel region af et antistof, og et prokaryot sekretionssignalpeptid, hvor den variable region under ekspression operabelt bindes ved dens N-ende til et prokaryot sekretionssignalpeptid til muliggørelse af sekretion fra værten; og (b) opnåelse af immunoglobulinkæden eller fragmentet af en immunoglobulin-kæde, som forud for sekretion fra værten oprindeligt var således bundet til signal-peptidet i form af en immunoglobulinmolekyle med tunge og lette kæder eller fragmenter deraf, at det overordnede molekyle udviser de ønskede bindings- og genkendelsesegenskaber. 2. Fremgangsmåde ifølge krav 1, hvor sekretionssignalpeptidet er et pektatlyase-sekretionssignal. 3. Fremgangsmåde ifølge krav 1 eller krav 2, hvor immunoglobulinmolekylet isoleres fra det periplasmiske rum. 4. Fremgangsmåde ifølge krav 1 eller krav 2, hvor immunoglobulinkæden eller fragmentet af en immunoglobulinkæde isoleres fra dyrkningsvækstmediet. 5. Fremgangsmåde ifølge et hvilket som helst af de foregående krav, hvor immu-noglobulinmolekylet er et Fd-firagment. 6. Fremgangsmåde ifølge et hvilket som helst af de foregående krav, hvor immu-noglobulinmolekylet omfatter en kimær immunoglobulinkæde. 7. Fremgangsmåde ifølge et hvilket som helst af kravene 1-4, hvor den variable region af antistof operabelt er bandet til en sekvens, som koder for et polypeptid, som er et andet end en immunoglobulinkæde. 8. Fremgangsmåde ifølge krav 7, hvor polypeptidet er et enzym.A process for producing an immunoglobulin molecule with heavy and light chains or fragments thereof, linked so that the parent molecule exhibits desired binding and recognition properties, the method comprising (a) cultivating a transformed or transfected prokaryotic host under appropriate conditions. conditions such that heavy chain immunoglobulin and light chain immunoglobulin or fragments thereof are expressed as a result of transcription of polynucleotide sequences in the host, wherein the polynucleotide sequences encode at least one functionally active region of a variable region of an antibody, and a prokaryotic secretion signal, region under expression operably binds at its N end to a prokaryotic secretion signal peptide to enable secretion from the host; and (b) obtaining the immunoglobulin chain or fragment of an immunoglobulin chain which, prior to secretion from the host, was thus initially bound to the signal peptide in the form of a heavy and light chain immunoglobulin molecule or the parent molecule exhibiting the desired bonding and recognition properties. The method of claim 1, wherein the secretion signal peptide is a pectal lyase secretion signal. The method of claim 1 or claim 2, wherein the immunoglobulin molecule is isolated from the periplasmic compartment. The method of claim 1 or claim 2, wherein the immunoglobulin chain or fragment of an immunoglobulin chain is isolated from the culture growth medium. A method according to any one of the preceding claims, wherein the immunoglobulin molecule is an Fd fragment. The method of any of the preceding claims, wherein the immunoglobulin molecule comprises a chimeric immunoglobulin chain. The method of any of claims 1-4, wherein the variable region of antibody is operably linked to a sequence encoding a polypeptide other than an immunoglobulin chain. The method of claim 7, wherein the polypeptide is an enzyme.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DK200301156A DK175581B1 (en) | 1987-07-24 | 2003-08-12 | Polynucleotide(s) encoding immunoglobulin molecules - used for efficient prodn. of chimeric human or non-human- or class switched-antibodies |
Applications Claiming Priority (6)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US7752887A | 1987-07-24 | 1987-07-24 | |
| US7752887 | 1987-07-24 | ||
| DK199000192A DK174824B1 (en) | 1987-07-24 | 1990-01-24 | Polynucleotide molecule, vector comprising polynucleotide molecule and prokaryotic host transfected with this vector |
| DK19290 | 1990-01-24 | ||
| DK200301156A DK175581B1 (en) | 1987-07-24 | 2003-08-12 | Polynucleotide(s) encoding immunoglobulin molecules - used for efficient prodn. of chimeric human or non-human- or class switched-antibodies |
| DK200301156 | 2003-08-12 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DK200301156A true DK200301156A (en) | 2003-08-12 |
| DK175581B1 DK175581B1 (en) | 2004-12-13 |
Family
ID=28043155
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK200301156A DK175581B1 (en) | 1987-07-24 | 2003-08-12 | Polynucleotide(s) encoding immunoglobulin molecules - used for efficient prodn. of chimeric human or non-human- or class switched-antibodies |
| DK200301155A DK175654B1 (en) | 1987-07-24 | 2003-08-12 | Polynucleotide(s) encoding immunoglobulin molecules - used for efficient prodn. of chimeric human or non-human- or class switched-antibodies |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK200301155A DK175654B1 (en) | 1987-07-24 | 2003-08-12 | Polynucleotide(s) encoding immunoglobulin molecules - used for efficient prodn. of chimeric human or non-human- or class switched-antibodies |
Country Status (1)
| Country | Link |
|---|---|
| DK (2) | DK175581B1 (en) |
-
2003
- 2003-08-12 DK DK200301156A patent/DK175581B1/en not_active IP Right Cessation
- 2003-08-12 DK DK200301155A patent/DK175654B1/en not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| DK175581B1 (en) | 2004-12-13 |
| DK175654B1 (en) | 2005-01-03 |
| DK200301155A (en) | 2003-08-12 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PUP | Patent expired |