DK1713930T3 - Hurtig screeningsmetode for translationsfusionspartnere til fremstilling af rekombinante proteiner og translationsfusionspartnere screenet derfra - Google Patents
Hurtig screeningsmetode for translationsfusionspartnere til fremstilling af rekombinante proteiner og translationsfusionspartnere screenet derfraInfo
- Publication number
- DK1713930T3 DK1713930T3 DK04808645T DK04808645T DK1713930T3 DK 1713930 T3 DK1713930 T3 DK 1713930T3 DK 04808645 T DK04808645 T DK 04808645T DK 04808645 T DK04808645 T DK 04808645T DK 1713930 T3 DK1713930 T3 DK 1713930T3
- Authority
- DK
- Denmark
- Prior art keywords
- fusion partners
- translation fusion
- screening method
- recombinant proteins
- produce recombinant
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
- C07K14/53—Colony-stimulating factor [CSF]
- C07K14/535—Granulocyte CSF; Granulocyte-macrophage CSF
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
- C07K14/54—Interleukins [IL]
- C07K14/55—IL-2
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/18—Carboxylic ester hydrolases (3.1.1)
- C12N9/20—Triglyceride splitting, e.g. by means of lipase
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/35—Fusion polypeptide containing a fusion for enhanced stability/folding during expression, e.g. fusions with chaperones or thioredoxin
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/61—Fusion polypeptide containing an enzyme fusion for detection (lacZ, luciferase)
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Gastroenterology & Hepatology (AREA)
- Toxicology (AREA)
- Bioinformatics & Computational Biology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Immunology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1020040003610A KR100626753B1 (ko) | 2004-01-17 | 2004-01-17 | 재조합 단백질 생산용 맞춤형 단백질융합인자의 초고속선별 방법 |
| KR1020040003957A KR20050076139A (ko) | 2004-01-19 | 2004-01-19 | 단백질 분비 생산을 유도하는 단백질융합인자 |
| PCT/KR2004/003517 WO2005068658A1 (en) | 2004-01-17 | 2004-12-30 | Rapid screening method of translational fusion partners for producing recombinant proteins and translational fusion partners screened therefrom |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK1713930T3 true DK1713930T3 (da) | 2011-01-17 |
Family
ID=34797866
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK10009260T DK2275556T3 (da) | 2004-01-17 | 2004-12-30 | Hurtig fremgangsmåde til screening af translationsfusionspartnere til fremstilling af rekombinante proteiner og translationsfusionpartnere screenet derfra |
| DK04808645T DK1713930T3 (da) | 2004-01-17 | 2004-12-30 | Hurtig screeningsmetode for translationsfusionspartnere til fremstilling af rekombinante proteiner og translationsfusionspartnere screenet derfra |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK10009260T DK2275556T3 (da) | 2004-01-17 | 2004-12-30 | Hurtig fremgangsmåde til screening af translationsfusionspartnere til fremstilling af rekombinante proteiner og translationsfusionpartnere screenet derfra |
Country Status (7)
| Country | Link |
|---|---|
| US (2) | US8673822B2 (da) |
| EP (2) | EP1713930B1 (da) |
| JP (2) | JP5148879B2 (da) |
| AT (1) | ATE486943T1 (da) |
| DE (1) | DE602004029936D1 (da) |
| DK (2) | DK2275556T3 (da) |
| WO (1) | WO2005068658A1 (da) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DK2275556T3 (da) | 2004-01-17 | 2013-07-08 | Korea Res Inst Of Bioscience | Hurtig fremgangsmåde til screening af translationsfusionspartnere til fremstilling af rekombinante proteiner og translationsfusionpartnere screenet derfra |
| KR20070009269A (ko) * | 2005-07-15 | 2007-01-18 | 한국생명공학연구원 | 재조합단백질 생산용 단백질융합인자 라이브러리 및이로부터 획득된 단백질융합인자 |
| EP2370470B1 (en) | 2008-12-04 | 2016-10-05 | Korea Research Institute of Bioscience and Biotechnology | Screening of abundantly secreted proteins and their use as fusion partners for the production of recombinant proteins |
| US8986956B2 (en) | 2010-11-04 | 2015-03-24 | Korea Research Institute Of Bioscience And Biotechnology | Method for producing human epidermal growth factor in large volume from yeast |
| EP3021834A1 (en) * | 2013-07-18 | 2016-05-25 | MannKind Corporation | Heat-stable dry powder pharmaceutical compositions and methods |
| CN104694523A (zh) * | 2015-03-21 | 2015-06-10 | 江南大学 | 一种精氨酸脱亚胺酶的胞外表达方法及其应用 |
| IL313740A (en) * | 2021-12-23 | 2024-08-01 | Helsingin Yliopisto | A method for screening a signal peptide for efficient expression and secretion of a heterologous polypeptide in mammalian cells |
Family Cites Families (26)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS60501140A (ja) * | 1983-04-22 | 1985-07-25 | アムジエン | 酵母による外因性ポリペプチドの分泌 |
| ES2061482T3 (es) * | 1986-05-02 | 1994-12-16 | Gist Brocades Nv | Metodo para la identificacion de secuencias de señal secretora para sintesis de proteina extracelular en bacillus. |
| FR2648713B1 (fr) * | 1989-06-26 | 1994-06-10 | Commissariat Energie Atomique | Proteines hybrides entre une enzyme extracytoplasmique et une autre proteine, leur procede de preparation, ainsi que leur application comme agent de diagnostic |
| US5212058A (en) * | 1990-05-09 | 1993-05-18 | Massachusetts Institute Of Technology | Nucleic acid encoding ubiquitin-specific proteases |
| US5547871A (en) * | 1993-01-25 | 1996-08-20 | American Cyanamid Company | Heterologous signal sequences for secretion of insect controlling proteins |
| US5536637A (en) * | 1993-04-07 | 1996-07-16 | Genetics Institute, Inc. | Method of screening for cDNA encoding novel secreted mammalian proteins in yeast |
| US5563046A (en) * | 1993-08-02 | 1996-10-08 | Celtrix Pharmaceuticals, Inc. | Fusion polypeptides and proteins |
| DE69426289T2 (de) * | 1993-12-10 | 2001-04-12 | Korea Institute Of Science And Technology, Seoul/Soul | Signalsequenzen für die Secretion heterologer Proteine von Hefe |
| JP3730256B2 (ja) * | 1995-02-03 | 2005-12-21 | 旭硝子株式会社 | 分泌シグナル遺伝子およびそれを有する発現ベクター |
| CA2252425A1 (en) | 1996-04-24 | 1997-10-30 | Genetics Institute, Inc. | Yeast invertase gene as reporter system for isolating cytokines |
| EP1170366A1 (en) | 1996-04-24 | 2002-01-09 | Genetics Institute, Inc. | Secreted proteins obtained by using Yeast invertase gene as reporter system |
| US5952171A (en) * | 1996-11-19 | 1999-09-14 | Millennium Biotherapeutics, Inc. | Method for identifying genes encoding secreted or membrane-associated proteins |
| US6136569A (en) * | 1997-02-27 | 2000-10-24 | Genentech, Inc. | Method of creating a cDNA library enriched in signal sequences |
| US20060009633A9 (en) * | 1997-11-13 | 2006-01-12 | Genset, S.A. | Complementary DNAs encoding proteins with signal peptides |
| WO2001000806A2 (en) * | 1999-06-25 | 2001-01-04 | Genset | Dna's encoding proteins with signal peptides |
| US6548633B1 (en) * | 1998-12-22 | 2003-04-15 | Genset, S.A. | Complementary DNA's encoding proteins with signal peptides |
| US6150098A (en) * | 1998-02-20 | 2000-11-21 | Amgen Inc. | Methods for identifying novel secreted mammalian polypeptides |
| AU3193399A (en) | 1998-03-23 | 1999-10-18 | Genentech Inc. | Method of selection for genes encoding secreted and transmembrane proteins |
| US6753314B1 (en) * | 1999-04-01 | 2004-06-22 | Curagen Corporation | Protein-protein complexes and methods of using same |
| ATE398173T1 (de) * | 2000-04-07 | 2008-07-15 | Novozymes As | Einfangen von signalsequenzen |
| US7029842B2 (en) * | 2000-04-07 | 2006-04-18 | Novozymes A/S | Signal sequence trapping |
| US20020127557A1 (en) * | 2001-03-09 | 2002-09-12 | Ruoying Tan | Method for identification of cDNAs encoding signal peptides |
| US7314974B2 (en) * | 2002-02-21 | 2008-01-01 | Monsanto Technology, Llc | Expression of microbial proteins in plants for production of plants with improved properties |
| DK2275556T3 (da) | 2004-01-17 | 2013-07-08 | Korea Res Inst Of Bioscience | Hurtig fremgangsmåde til screening af translationsfusionspartnere til fremstilling af rekombinante proteiner og translationsfusionpartnere screenet derfra |
| KR20070009269A (ko) * | 2005-07-15 | 2007-01-18 | 한국생명공학연구원 | 재조합단백질 생산용 단백질융합인자 라이브러리 및이로부터 획득된 단백질융합인자 |
| EP2370470B1 (en) * | 2008-12-04 | 2016-10-05 | Korea Research Institute of Bioscience and Biotechnology | Screening of abundantly secreted proteins and their use as fusion partners for the production of recombinant proteins |
-
2004
- 2004-12-30 DK DK10009260T patent/DK2275556T3/da active
- 2004-12-30 AT AT04808645T patent/ATE486943T1/de not_active IP Right Cessation
- 2004-12-30 US US10/586,045 patent/US8673822B2/en active Active
- 2004-12-30 EP EP04808645A patent/EP1713930B1/en not_active Expired - Lifetime
- 2004-12-30 DK DK04808645T patent/DK1713930T3/da active
- 2004-12-30 EP EP20100009260 patent/EP2275556B1/en not_active Expired - Lifetime
- 2004-12-30 WO PCT/KR2004/003517 patent/WO2005068658A1/en active Application Filing
- 2004-12-30 DE DE200460029936 patent/DE602004029936D1/de not_active Expired - Lifetime
- 2004-12-30 JP JP2006549111A patent/JP5148879B2/ja not_active Expired - Fee Related
-
2012
- 2012-03-01 JP JP2012045852A patent/JP5662363B2/ja not_active Expired - Fee Related
-
2014
- 2014-01-30 US US14/168,405 patent/US9012367B2/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| DE602004029936D1 (de) | 2010-12-16 |
| WO2005068658A1 (en) | 2005-07-28 |
| US20140220631A1 (en) | 2014-08-07 |
| JP5148879B2 (ja) | 2013-02-20 |
| EP1713930B1 (en) | 2010-11-03 |
| EP1713930A4 (en) | 2007-02-28 |
| JP2007517519A (ja) | 2007-07-05 |
| DK2275556T3 (da) | 2013-07-08 |
| EP2275556B1 (en) | 2013-04-24 |
| ATE486943T1 (de) | 2010-11-15 |
| EP2275556A1 (en) | 2011-01-19 |
| JP5662363B2 (ja) | 2015-01-28 |
| US20070275385A1 (en) | 2007-11-29 |
| EP1713930A1 (en) | 2006-10-25 |
| JP2012105675A (ja) | 2012-06-07 |
| US8673822B2 (en) | 2014-03-18 |
| US9012367B2 (en) | 2015-04-21 |
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