[go: up one dir, main page]

DK1694361T3 - Targeted delivery to non-phagocytic mammalian cells via bacterially derived intact minicells - Google Patents

Targeted delivery to non-phagocytic mammalian cells via bacterially derived intact minicells

Info

Publication number
DK1694361T3
DK1694361T3 DK04806560.1T DK04806560T DK1694361T3 DK 1694361 T3 DK1694361 T3 DK 1694361T3 DK 04806560 T DK04806560 T DK 04806560T DK 1694361 T3 DK1694361 T3 DK 1694361T3
Authority
DK
Denmark
Prior art keywords
mammalian cells
phagocytic
minicells
phagocytic mammalian
cells via
Prior art date
Application number
DK04806560.1T
Other languages
Danish (da)
Inventor
Himanshu Brahmbhatt
Jennifer Macdiarmid
Original Assignee
Engeneic Molecular Delivery Pty Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Engeneic Molecular Delivery Pty Ltd filed Critical Engeneic Molecular Delivery Pty Ltd
Application granted granted Critical
Publication of DK1694361T3 publication Critical patent/DK1694361T3/en

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • A61K48/0008—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy characterised by an aspect of the 'non-active' part of the composition delivered, e.g. wherein such 'non-active' part is not delivered simultaneously with the 'active' part of the composition
    • A61K48/0025—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy characterised by an aspect of the 'non-active' part of the composition delivered, e.g. wherein such 'non-active' part is not delivered simultaneously with the 'active' part of the composition wherein the non-active part clearly interacts with the delivered nucleic acid
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/395—Antibodies; Immunoglobulins; Immune serum, e.g. antilymphocytic serum
    • A61K39/39533—Antibodies; Immunoglobulins; Immune serum, e.g. antilymphocytic serum against materials from animals
    • A61K39/3955—Antibodies; Immunoglobulins; Immune serum, e.g. antilymphocytic serum against materials from animals against proteinaceous materials, e.g. enzymes, hormones, lymphokines
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
    • A61K47/69—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit
    • A61K47/6901—Conjugates being cells, cell fragments, viruses, ghosts, red blood cells or viral vectors
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • A61K48/0008—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy characterised by an aspect of the 'non-active' part of the composition delivered, e.g. wherein such 'non-active' part is not delivered simultaneously with the 'active' part of the composition
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • A61K48/0008—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy characterised by an aspect of the 'non-active' part of the composition delivered, e.g. wherein such 'non-active' part is not delivered simultaneously with the 'active' part of the composition
    • A61K48/0025—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy characterised by an aspect of the 'non-active' part of the composition delivered, e.g. wherein such 'non-active' part is not delivered simultaneously with the 'active' part of the composition wherein the non-active part clearly interacts with the delivered nucleic acid
    • A61K48/0041—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy characterised by an aspect of the 'non-active' part of the composition delivered, e.g. wherein such 'non-active' part is not delivered simultaneously with the 'active' part of the composition wherein the non-active part clearly interacts with the delivered nucleic acid the non-active part being polymeric
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P11/00—Drugs for disorders of the respiratory system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P15/00—Drugs for genital or sexual disorders; Contraceptives
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P19/00—Drugs for skeletal disorders
    • A61P19/04—Drugs for skeletal disorders for non-specific disorders of the connective tissue
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A61P31/14—Antivirals for RNA viruses
    • A61P31/18—Antivirals for RNA viruses for HIV
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/88—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation using microencapsulation, e.g. using amphiphile liposome vesicle
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
    • A61K2039/52—Bacterial cells; Fungal cells; Protozoal cells
    • A61K2039/523—Bacterial cells; Fungal cells; Protozoal cells expressing foreign proteins
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2318/00—Antibody mimetics or scaffolds
    • C07K2318/10—Immunoglobulin or domain(s) thereof as scaffolds for inserted non-Ig peptide sequences, e.g. for vaccination purposes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/035—Fusion polypeptide containing a localisation/targetting motif containing a signal for targeting to the external surface of a cell, e.g. to the outer membrane of Gram negative bacteria, GPI- anchored eukaryote proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2810/00—Vectors comprising a targeting moiety
    • C12N2810/50—Vectors comprising as targeting moiety peptide derived from defined protein
    • C12N2810/80—Vectors comprising as targeting moiety peptide derived from defined protein from vertebrates
    • C12N2810/85—Vectors comprising as targeting moiety peptide derived from defined protein from vertebrates mammalian
    • C12N2810/851—Vectors comprising as targeting moiety peptide derived from defined protein from vertebrates mammalian from growth factors; from growth regulators
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2810/00—Vectors comprising a targeting moiety
    • C12N2810/50—Vectors comprising as targeting moiety peptide derived from defined protein
    • C12N2810/80—Vectors comprising as targeting moiety peptide derived from defined protein from vertebrates
    • C12N2810/85—Vectors comprising as targeting moiety peptide derived from defined protein from vertebrates mammalian
    • C12N2810/859—Vectors comprising as targeting moiety peptide derived from defined protein from vertebrates mammalian from immunoglobulins

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Epidemiology (AREA)
  • Molecular Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Immunology (AREA)
  • Virology (AREA)
  • Microbiology (AREA)
  • Hematology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Endocrinology (AREA)
  • Mycology (AREA)
  • Communicable Diseases (AREA)
  • Diabetes (AREA)
  • Obesity (AREA)
  • Oncology (AREA)
  • Pulmonology (AREA)
  • Physical Education & Sports Medicine (AREA)

Abstract

A method of targeting bacterially-derived, intact minicells to specific, non-phagocytic mammalian cells employs bispecific ligands to deliver nucleic acids efficiently to the mammalian cells. Bispecific ligands, comprising (i) a first arm that carries specificity for a bacterially-derived minicel surface structure and (ii) a second arm that carries specificity for a non-phagocytic mammalian cell surface receptor are useful for targeting minicells to specific, non-phagocytic mammalian cells and causing endocytosis of minicells by non-phagocytic cells.
DK04806560.1T 2003-12-09 2004-12-08 Targeted delivery to non-phagocytic mammalian cells via bacterially derived intact minicells DK1694361T3 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US52776403P 2003-12-09 2003-12-09
PCT/IB2004/004406 WO2005056749A2 (en) 2003-12-09 2004-12-08 Targeted gene delivery to non-phagocytic mammalian cells via bacterially derived intact minicells

Publications (1)

Publication Number Publication Date
DK1694361T3 true DK1694361T3 (en) 2011-06-06

Family

ID=34676775

Family Applications (1)

Application Number Title Priority Date Filing Date
DK04806560.1T DK1694361T3 (en) 2003-12-09 2004-12-08 Targeted delivery to non-phagocytic mammalian cells via bacterially derived intact minicells

Country Status (15)

Country Link
US (3) US9169495B2 (en)
EP (2) EP2295084A1 (en)
JP (1) JP4332558B2 (en)
CN (2) CN1913923B (en)
AT (1) ATE501735T1 (en)
AU (1) AU2004297414C1 (en)
CA (1) CA2549840C (en)
DE (1) DE602004031870D1 (en)
DK (1) DK1694361T3 (en)
ES (1) ES2362881T3 (en)
HK (1) HK1095090A1 (en)
NZ (1) NZ547983A (en)
PL (1) PL1694361T3 (en)
PT (1) PT1694361E (en)
WO (1) WO2005056749A2 (en)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2775218C (en) * 2001-10-15 2016-04-26 Engeneic Molecular Delivery Pty Ltd. Intact minicells as vectors for dna transfer and gene therapy in vitro and in vivo
US7611885B2 (en) * 2003-06-24 2009-11-03 Engeneic Molecular Delivery Pty, Ltd. Pharmaceutically compatible method for purifying intact bacterial minicells
DK1694361T3 (en) 2003-12-09 2011-06-06 Engeneic Molecular Delivery Pty Ltd Targeted delivery to non-phagocytic mammalian cells via bacterially derived intact minicells
US8772013B2 (en) * 2004-02-02 2014-07-08 Engeneic Molecular Delivery Pty Ltd Methods for targeted in vitro and in vivo drug delivery to mammalian cells via bacterially derived intact minicells
HUE025292T2 (en) 2004-02-02 2016-02-29 Engeneic Molecular Delivery Pty Ltd Compositions and methods for targeted in vitro and in vivo drug delivery to mammalian cells via bacterially derived intact minicells
PL2386640T3 (en) * 2004-08-26 2015-08-31 Engeneic Molecular Delivery Pty Ltd Delivering functional nucleic acids to mammalian cells via bacterially-derived, intact minicells
SI2712618T1 (en) 2006-06-23 2017-02-28 Engeneic Molecular Delivery Pty Ltd. Targeted delivery of drugs, therapeutic nucleic acids and functional nucleic acids to mammalian cells via intact killed bacterial cells
JP5167338B2 (en) 2007-03-30 2013-03-21 エンジーンアイシー モレキュラー デリバリー ピーティーワイ リミテッド Intact bacterially derived minicells containing plasmid-free functional nucleic acids for in vivo delivery to mammalian cells
US20100112670A1 (en) 2008-06-25 2010-05-06 Vaxiion Therapeutics, Inc. Regulated genetic suicide mechanism compositions and methods
EP3527222B1 (en) 2011-02-15 2021-04-07 Vaxiion Therapeutics, LLC Therapeutic compositions and methods for antibody and fc-containing targeting molecule-based targeted delivery of bioactive molecules by bacterial minicells
SG10201601349XA (en) 2011-12-13 2016-03-30 Engeneic Molecular Delivery Pty Ltd Bacterially derived, intact minicells for delivery of therapeutic agents to brain tumors
ES2828092T3 (en) 2012-10-02 2021-05-25 Vaxiion Therapeutics Llc Immunomodulatory minicells and methods of use
US9017698B2 (en) * 2013-09-25 2015-04-28 Sequoia Sciences, Inc. Compositions of vaccines and adjuvants and methods for the treatment of urinary tract infections
CN105658233B (en) * 2013-10-04 2020-09-04 安吉尼科分子传输公司 Combined tumor therapy using drug-carrying, dual-specific ligand-targeted minicells and interferon-gamma
AU2015326407C1 (en) 2014-10-03 2021-06-24 Engeneic Molecular Delivery Pty Ltd Enhanced loading of intact, bacterially derived vesicles with small molecule compounds
WO2017024059A1 (en) 2015-08-04 2017-02-09 Vaxiion Therapeutics, Llc Ionizing irradiation sterilization of bacterial minicell-based biopharmaceuticals and methods of use
US20190328783A1 (en) * 2016-04-15 2019-10-31 Cellectis A method of engineering prodrug-specific hypersensitive t-cells for immunotherapy by gene expression
WO2017178585A1 (en) 2016-04-15 2017-10-19 Cellectis A method of engineering drug-specific hypersensitive t-cells for immunotherapy by gene inactivation
CA3039010A1 (en) 2016-10-06 2018-04-12 Engeneic Molecular Delivery Pty Ltd Bacterial minicells for delivering nucleic acid adjuvants and methods of using the same
WO2018098163A1 (en) 2016-11-23 2018-05-31 Vaxiion Therapeutics, Llc Immunomodulatory & oncolytic minicells and methods of use
CA3107095A1 (en) 2018-07-23 2020-01-30 Engeneic Molecular Delivery Pty Ltd Compositions comprising bacterially derived minicells and methods of using the same
WO2020222161A1 (en) * 2019-05-01 2020-11-05 Engeneic Molecular Delivery Pty Ltd Compositions comprising bacterially derived intact minicells for theranostic applications

Family Cites Families (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4496778A (en) 1983-10-03 1985-01-29 Exxon Research & Engineering Co. Process for the hydroxylation of olefins using molecular oxygen, an osmium containing catalyst, a copper Co-catalyst, and an aromatic amine based promoter
GB8611832D0 (en) 1986-05-15 1986-06-25 Holland I B Polypeptide
US5260203A (en) 1986-09-02 1993-11-09 Enzon, Inc. Single polypeptide chain binding molecules
US5037743A (en) 1988-08-05 1991-08-06 Zymogenetics, Inc. BAR1 secretion signal
US5530101A (en) 1988-12-28 1996-06-25 Protein Design Labs, Inc. Humanized immunoglobulins
US5221479A (en) * 1991-02-15 1993-06-22 Fuji Photo Film Co., Ltd. Filtration system
DE122004000008I1 (en) 1991-06-14 2005-06-09 Genentech Inc Humanized heregulin antibody.
WO1995021191A1 (en) 1994-02-04 1995-08-10 William Ward Bioluminescent indicator based upon the expression of a gene for a modified green-fluorescent protein
NZ288883A (en) 1994-07-11 1998-12-23 Univ Texas Conjugates comprising coagulation factors
WO1998041628A1 (en) 1997-03-19 1998-09-24 Zymogenetics, Inc. Secreted salivary zsig32 polypeptides
US20030004123A1 (en) * 1997-06-26 2003-01-02 Boucher Richard C. Targeted gene transfer using G protein coupled receptors
AU754425B2 (en) * 1997-09-15 2002-11-14 Genetic Immunity, Llc. Method of delivering genes to antigen presenting cells of the skin
US6225113B1 (en) 1998-12-04 2001-05-01 Genvec, Inc. Use of trans-activation and cis-activation to modulate the persistence of expression of a transgene in an at least E4-deficient adenovirus
EP2363478B1 (en) 1999-04-21 2019-07-24 Alnylam Pharmaceuticals, Inc. Methods and compositions for inhibiting the function of polynucleotide sequences
US6635448B2 (en) 2000-08-21 2003-10-21 Clonexdevelopment, Inc. Methods and compositions for increasing protein yield from a cell culture
NZ528250A (en) * 2001-03-07 2006-09-29 Childrens Medical Center Method to screen peptide display libraries using minicell display
US7011946B2 (en) 2001-05-22 2006-03-14 Trustees Of Tufts College In vivo assay for identification of antimicrobial agents
US20030194798A1 (en) * 2001-05-24 2003-10-16 Surber Mark W. Minicell compositions and methods
US20030194714A1 (en) 2001-06-05 2003-10-16 Sabbadini Roger A. Minicell-based transformation
EP1399484B1 (en) 2001-06-28 2010-08-11 Domantis Limited Dual-specific ligand and its use
CA2775218C (en) 2001-10-15 2016-04-26 Engeneic Molecular Delivery Pty Ltd. Intact minicells as vectors for dna transfer and gene therapy in vitro and in vivo
US20030203481A1 (en) * 2002-02-25 2003-10-30 Surber Mark W. Conjugated minicells
ES2541351T3 (en) * 2002-02-25 2015-07-17 Vaxiion Therapeutics, Llc Compositions of mini cells and procedures
US7611885B2 (en) 2003-06-24 2009-11-03 Engeneic Molecular Delivery Pty, Ltd. Pharmaceutically compatible method for purifying intact bacterial minicells
DK1694361T3 (en) 2003-12-09 2011-06-06 Engeneic Molecular Delivery Pty Ltd Targeted delivery to non-phagocytic mammalian cells via bacterially derived intact minicells
US8772013B2 (en) 2004-02-02 2014-07-08 Engeneic Molecular Delivery Pty Ltd Methods for targeted in vitro and in vivo drug delivery to mammalian cells via bacterially derived intact minicells
PL2386640T3 (en) 2004-08-26 2015-08-31 Engeneic Molecular Delivery Pty Ltd Delivering functional nucleic acids to mammalian cells via bacterially-derived, intact minicells
JP5601756B2 (en) 2004-12-17 2014-10-08 ベス イスラエル デアコネス メディカル センター， インコーポレイテッド Compositions for bacteria-mediated gene silencing and methods of use thereof
SI2712618T1 (en) 2006-06-23 2017-02-28 Engeneic Molecular Delivery Pty Ltd. Targeted delivery of drugs, therapeutic nucleic acids and functional nucleic acids to mammalian cells via intact killed bacterial cells

Also Published As

Publication number Publication date
JP2007534310A (en) 2007-11-29
US9987377B2 (en) 2018-06-05
CN1913923A (en) 2007-02-14
EP1694361A4 (en) 2006-12-13
US20070237744A1 (en) 2007-10-11
HK1095090A1 (en) 2007-04-27
AU2004297414B2 (en) 2008-10-30
WO2005056749A2 (en) 2005-06-23
DE602004031870D1 (en) 2011-04-28
ES2362881T3 (en) 2011-07-14
NZ547983A (en) 2009-05-31
PT1694361E (en) 2011-06-27
CA2549840C (en) 2012-03-20
JP4332558B2 (en) 2009-09-16
CA2549840A1 (en) 2005-06-23
CN101954095B (en) 2013-03-13
CN1913923B (en) 2010-12-08
AU2004297414C1 (en) 2009-06-25
AU2004297414A1 (en) 2005-06-23
EP2295084A1 (en) 2011-03-16
PL1694361T3 (en) 2011-08-31
WO2005056749A3 (en) 2005-10-13
US20150343092A1 (en) 2015-12-03
EP1694361A2 (en) 2006-08-30
ATE501735T1 (en) 2011-04-15
US9169495B2 (en) 2015-10-27
CN101954095A (en) 2011-01-26
EP1694361B1 (en) 2011-03-16
US20180369416A1 (en) 2018-12-27
US10583200B2 (en) 2020-03-10

Similar Documents

Publication Publication Date Title
HK1095090A1 (en) Targeted gene delivery to non-phagocytic mammalian cells via bacterially derived intact minicells
CY1118437T1 (en) Targeted Release of Therapeutic Nucleic Acids and Functional Nucleic Acids in Mammalian Unconventional Thyroid Cells
HK1163160A1 (en) Delivering functional nucleic acids to mammalian cells via bacterially- derived, intact minicells
ATE536418T1 (en) LIPID ENCAPSULATED INTERFERENCE RNA
DK1525320T3 (en) Method for Expression of Recombinant Protein in CHO Cells
ATE465250T1 (en) ACID ALPHA-GLUCOSIDASE AND FRAGMENTS THEREOF
DK2025756T3 (en) Improved targeted DNA insertion in plants
BR0115558A (en) isolated nucleic acid, recombinant expression construct, yeast cell transformed with recombinant expression construct, and method for producing lactic acid
WO2005117991A3 (en) Compositions and methods for enhancing delivery of nucleic acids into cells and for modifying expression of target genes in cells
SG135174A1 (en) Compositions and methods for targeted in vitro and in vivo drug delivery to mammalian cells via bacterially derived intact minicells
WO2007016507A3 (en) Multivalent rna nanoparticles for delivery of active agents to a cell
AR069253A1 (en) TRANSGENIC PLANTS EXPRESSING THE NUCLEIC ACID OF OSSIK1 AND MODULATORS TO INCREASE TOLERANCE TO STRESS
DE602004022778D1 (en) MESENCHYMAL STEM CELLS AS A VEHICLE FOR ION CHANNEL TRANSFER IN SYNZYTIC STRUCTURES
AU2014208251B2 (en) Carbohydrate conjugates as delivery agents for oligonucleotides
NZ590562A (en) Method for cloning avian-derived antibodies
GB9816138D0 (en) Organic compounds
DK1554374T3 (en) Method for large-scale T lymphocyte culture in a homogeneous system
MXPA03011013A (en) Nucleic acid constructs useful for glucose regulated production of human insulin in somatic cell lines.
WO2007117852A3 (en) Targeted deletions using retroelement-mediated placement of recombination sites
BRPI0308659B8 (en) nucleic acid sequences, chimeric gene, vector, bacillus thuringiensis strain, as well as plant protection process against insect damage.
ATE439143T1 (en) HM1.24 EXPLOITABLE CANCER VACCINE
EP1570051A4 (en) HYBRID GENES AND COMPOSITIONS COMPRISING RRE AND CTE AND USES THEREOF
ATE428783T1 (en) EXPRESSION SYSTEM FOR SIRNA