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DK1567652T3 - New neomycin phosphotransferase genes and method for selecting high-producing recombinant cells - Google Patents

New neomycin phosphotransferase genes and method for selecting high-producing recombinant cells

Info

Publication number
DK1567652T3
DK1567652T3 DK03812157T DK03812157T DK1567652T3 DK 1567652 T3 DK1567652 T3 DK 1567652T3 DK 03812157 T DK03812157 T DK 03812157T DK 03812157 T DK03812157 T DK 03812157T DK 1567652 T3 DK1567652 T3 DK 1567652T3
Authority
DK
Denmark
Prior art keywords
neomycin phosphotransferase
recombinant cells
producing recombinant
selecting high
phosphotransferase genes
Prior art date
Application number
DK03812157T
Other languages
Danish (da)
Inventor
Barbara Enenkel
Kerstin Dr Sautter
Ralf Otto
Juergen Fieder
Klaus Bergemann
Original Assignee
Boehringer Ingelheim Pharma
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DE10330686A external-priority patent/DE10330686A1/en
Application filed by Boehringer Ingelheim Pharma filed Critical Boehringer Ingelheim Pharma
Application granted granted Critical
Publication of DK1567652T3 publication Critical patent/DK1567652T3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/65—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/01—Phosphotransferases with an alcohol group as acceptor (2.7.1)
    • C12Y207/01095—Kanamycin kinase (2.7.1.95), i.e. neomycin-kanamycin phosphotransferase

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The invention relates to novel modified neomycin phosphotransferase genes and the use thereof in a selection method for high-producing recombinant cells. The invention also relates to expression vectors containing a modified neomycin phosphotransferase gene and a gene of interest in a functional bond with a heterologous promoter, and a method for producing heterologous gene products by using said expression vectors.
DK03812157T 2002-11-29 2003-11-25 New neomycin phosphotransferase genes and method for selecting high-producing recombinant cells DK1567652T3 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE10256081 2002-11-29
DE10330686A DE10330686A1 (en) 2002-11-29 2003-07-08 New neomycin phosphotransferase genes and methods for the selection of high-producing recombinant cells
PCT/EP2003/013203 WO2004050884A2 (en) 2002-11-29 2003-11-25 Novel neomycin phosphotransferase genes and method for the selection of high-producing recombinant cells

Publications (1)

Publication Number Publication Date
DK1567652T3 true DK1567652T3 (en) 2007-11-26

Family

ID=32471486

Family Applications (1)

Application Number Title Priority Date Filing Date
DK03812157T DK1567652T3 (en) 2002-11-29 2003-11-25 New neomycin phosphotransferase genes and method for selecting high-producing recombinant cells

Country Status (12)

Country Link
EP (1) EP1567652B1 (en)
JP (1) JP4344325B2 (en)
KR (1) KR101149777B1 (en)
AT (1) ATE371035T1 (en)
AU (1) AU2003302689B2 (en)
BR (1) BR0316525A (en)
CA (1) CA2507664C (en)
DE (1) DE50308030D1 (en)
DK (1) DK1567652T3 (en)
ES (1) ES2293096T3 (en)
MX (1) MXPA05005481A (en)
WO (1) WO2004050884A2 (en)

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US20080124760A1 (en) 2006-07-26 2008-05-29 Barbara Enenkel Regulatory Nucleic Acid Elements
EP1975228A1 (en) * 2007-03-28 2008-10-01 Fachhochschule Mannheim Polynucleotides for enhancing expression of a polynucleotide of interest
ES2552325T3 (en) * 2008-12-22 2015-11-27 National University Corporation Hokkaido University Expression vector for large-scale production of protein derived from foreign genes using animal cells, and use thereof
JP4547643B1 (en) * 2009-06-05 2010-09-22 東洋紡績株式会社 Expression vector optimized for cloning
JP4525863B1 (en) 2009-06-05 2010-08-18 東洋紡績株式会社 Expression vector for establishment of high-productivity cells and high-productivity cells
JP4491808B1 (en) * 2009-06-05 2010-06-30 東洋紡績株式会社 Drug selection marker gene expression cassette useful for efficient selection of high-expressing cells
US20120258496A1 (en) 2010-09-27 2012-10-11 Boehringer Ingelheim International Gmbh Production of low fucose antibodies in h4-ii-e rat cells
JP5775974B2 (en) 2011-10-28 2015-09-09 プロセナ バイオサイエンシーズ リミテッド Humanized antibodies that recognize alpha synuclein
CN104619724B (en) 2012-01-27 2018-05-04 普罗典娜生物科学有限公司 Identify the humanized antibody of alpha-synapse nucleoprotein
EP3536789A1 (en) 2012-06-06 2019-09-11 Boehringer Ingelheim International GmbH Cell engineering using rnas
UA118441C2 (en) 2012-10-08 2019-01-25 Протена Біосаєнсиз Лімітед Antibodies recognizing alpha-synuclein
EP2938726B1 (en) 2012-12-31 2017-09-27 Boehringer Ingelheim International GmbH Heterologous intron within a signal peptide
WO2014102101A1 (en) 2012-12-31 2014-07-03 Boehringer Ingelheim International Gmbh Novel intron sequences
EP3689904A1 (en) 2013-03-13 2020-08-05 Prothena Biosciences Limited Tau immunotherapy
US10513555B2 (en) 2013-07-04 2019-12-24 Prothena Biosciences Limited Antibody formulations and methods
WO2015004633A1 (en) 2013-07-12 2015-01-15 Neotope Biosciences Limited Antibodies that recognize islet-amyloid polypeptide (iapp)
US9850302B2 (en) 2013-07-12 2017-12-26 Prothena Biosciences Limited Antibodies that recognize IAPP
US11191832B2 (en) 2013-11-19 2021-12-07 Prothena Biosciences Limited Monitoring immunotherapy of Lewy body disease from constipation symptoms
EP3116907A1 (en) 2014-03-12 2017-01-18 Prothena Biosciences Limited Anti-laminin4 antibodies specific for lg4-5
EP3116906A1 (en) 2014-03-12 2017-01-18 Prothena Biosciences Limited Anti-laminin4 antibodies specific for lg1-3
ES2744526T3 (en) 2014-03-12 2020-02-25 Prothena Biosciences Ltd Anti-MCAM antibodies and associated methods of use
TW201623331A (en) 2014-03-12 2016-07-01 普羅帝納生物科學公司 Anti-MCAM antibodies and associated methods of use
US10562973B2 (en) 2014-04-08 2020-02-18 Prothena Bioscience Limited Blood-brain barrier shuttles containing antibodies recognizing alpha-synuclein
TWI718121B (en) 2015-01-28 2021-02-11 愛爾蘭商普羅佘納生物科技有限公司 Anti-transthyretin antibodies
TWI786505B (en) 2015-01-28 2022-12-11 愛爾蘭商普羅佘納生物科技有限公司 Anti-transthyretin antibodies
TWI711631B (en) 2015-01-28 2020-12-01 愛爾蘭商普羅佘納生物科技有限公司 Anti-transthyretin antibodies
WO2017046776A2 (en) 2015-09-16 2017-03-23 Prothena Biosciences Limited Use of anti-mcam antibodies for treatment or prophylaxis of giant cell arteritis, polymyalgia rheumatica or takayasu's arteritis
WO2017046774A2 (en) 2015-09-16 2017-03-23 Prothena Biosciences Limited Use of anti-mcam antibodies for treatment or prophylaxis of giant cell arteritis, polymyalgia rheumatica or takayasu's arteritis
WO2017149513A1 (en) 2016-03-03 2017-09-08 Prothena Biosciences Limited Anti-mcam antibodies and associated methods of use
WO2017153955A1 (en) 2016-03-09 2017-09-14 Prothena Biosciences Limited Use of anti-mcam antibodies for treatment or prophylaxis of granulomatous lung diseases
WO2017153953A1 (en) 2016-03-09 2017-09-14 Prothena Biosciences Limited Use of anti-mcam antibodies for treatment or prophylaxis of granulomatous lung diseases
EA201892412A1 (en) 2016-05-02 2019-05-31 Протена Биосайенсис Лимитед ANTIBODIES RECOGNIZING TAU
PL3452507T3 (en) 2016-05-02 2023-01-09 Prothena Biosciences Limited Tau immunotherapy
JP7481726B2 (en) 2016-05-02 2024-05-13 プロセナ バイオサイエンシーズ リミテッド Tau recognition antibody
WO2017208210A1 (en) 2016-06-03 2017-12-07 Prothena Biosciences Limited Anti-mcam antibodies and associated methods of use
EP3478714A2 (en) 2016-07-02 2019-05-08 Prothena Biosciences Limited Anti-transthyretin antibodies
WO2018007923A2 (en) 2016-07-02 2018-01-11 Prothena Biosciences Limited Anti-transthyretin antibodies
JP7017013B2 (en) 2016-07-02 2022-02-08 プロセナ バイオサイエンシーズ リミテッド Anti-transthyretin antibody
CN110023500B (en) * 2016-11-16 2024-10-11 新加坡科技研究局 Attenuated glutamine synthetases as selectable markers
AU2018263935B2 (en) 2017-05-02 2024-09-26 Prothena Biosciences Limited Antibodies recognizing tau
AU2017434556B2 (en) 2017-09-28 2024-11-14 F. Hoffmann-La Roche Ag Dosing regimes for treatment of synucleinopathies
PE20210042A1 (en) 2017-10-06 2021-01-08 Prothena Biosciences Ltd METHODS TO DETECT TRANSTIRETIN
CA3117816A1 (en) 2018-11-26 2020-06-04 Forty Seven, Inc. Humanized antibodies against c-kit
AU2020231366A1 (en) 2019-03-03 2021-08-12 Prothena Biosciences Limited Antibodies recognizing tau
EP4133086A4 (en) * 2020-04-07 2024-06-05 IO Biosciences, Inc. Nucleic acid constructs comprising gene editing multi-sites
WO2021263279A1 (en) 2020-06-24 2021-12-30 Prothena Biosciences Limited Antibodies recognizing sortilin

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JPH04179485A (en) * 1990-11-09 1992-06-26 Junichi Miyazaki New vector and method for selecting highly expressing transformed cell using the same vector
JP2004179485A (en) 2002-11-28 2004-06-24 Matsushita Electric Ind Co Ltd Printed wiring board and method of manufacturing the same

Also Published As

Publication number Publication date
MXPA05005481A (en) 2005-07-25
WO2004050884A2 (en) 2004-06-17
EP1567652A2 (en) 2005-08-31
BR0316525A (en) 2005-10-04
JP2006507842A (en) 2006-03-09
KR20050085202A (en) 2005-08-29
EP1567652B1 (en) 2007-08-22
AU2003302689A1 (en) 2004-06-23
KR101149777B1 (en) 2012-06-11
CA2507664A1 (en) 2004-06-17
CA2507664C (en) 2010-09-07
JP4344325B2 (en) 2009-10-14
ATE371035T1 (en) 2007-09-15
ES2293096T3 (en) 2008-03-16
DE50308030D1 (en) 2007-10-04
WO2004050884A3 (en) 2004-07-15
AU2003302689B2 (en) 2009-02-05

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