DK1201749T3 - Fremgangsmåde til fremstilling af humane bruskimplantater ved hjælp af in vitro dyrkede chondrocytter - Google Patents

Fremgangsmåde til fremstilling af humane bruskimplantater ved hjælp af in vitro dyrkede chondrocytter

Info

Publication number: DK1201749T3
Authority: DK; Denmark
Prior art keywords: preparation; chondrocytes; human cartilage; vitro cultured; cultured chondrocytes
Prior art date: 2000-08-29

Application number

DK01117785T

Other languages

English (en)

Inventor

Brigitte Dr Jeschke

Joerg Dr Meyer

Peter Dr Adamietz

Norbert Dr Meenen

Christiane Goepfert

Original Assignee

Biomet Deutschland Gmbh

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

2000-08-29

Filing date

2001-08-02

Publication date

2008-01-07

2001-08-02 Application filed by Biomet Deutschland Gmbh filed Critical Biomet Deutschland Gmbh

2008-01-07 Application granted granted Critical

2008-01-07 Publication of DK1201749T3 publication Critical patent/DK1201749T3/da

Classifications

- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/20—Polysaccharides
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
- A61L27/3804—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells
- A61L27/3817—Cartilage-forming cells, e.g. pre-chondrocytes
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
- A61L27/3839—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by the site of application in the body
- A61L27/3843—Connective tissue
- A61L27/3852—Cartilage, e.g. meniscus
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
- A61L27/3895—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells using specific culture conditions, e.g. stimulating differentiation of stem cells, pulsatile flow conditions
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/52—Hydrogels or hydrocolloids
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0655—Chondrocytes; Cartilage
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2430/00—Materials or treatment for tissue regeneration
- A61L2430/06—Materials or treatment for tissue regeneration for cartilage reconstruction, e.g. meniscus
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/30—Organic components
- C12N2500/38—Vitamins
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/105—Insulin-like growth factors [IGF]
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/11—Epidermal growth factor [EGF]
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/15—Transforming growth factor beta (TGF-β)

Landscapes

Health & Medical Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Engineering & Computer Science (AREA)
Biomedical Technology (AREA)
Chemical & Material Sciences (AREA)
General Health & Medical Sciences (AREA)
Oral & Maxillofacial Surgery (AREA)
Transplantation (AREA)
Epidemiology (AREA)
Dermatology (AREA)
Animal Behavior & Ethology (AREA)
Public Health (AREA)
Veterinary Medicine (AREA)
Cell Biology (AREA)
Zoology (AREA)
Medicinal Chemistry (AREA)
Botany (AREA)
Chemical Kinetics & Catalysis (AREA)
Rheumatology (AREA)
Genetics & Genomics (AREA)
Bioinformatics & Cheminformatics (AREA)
Organic Chemistry (AREA)
Wood Science & Technology (AREA)
Biotechnology (AREA)
Microbiology (AREA)
Dispersion Chemistry (AREA)
Vascular Medicine (AREA)
Developmental Biology & Embryology (AREA)
Biochemistry (AREA)
General Engineering & Computer Science (AREA)
Urology & Nephrology (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Materials For Medical Uses (AREA)
Prostheses (AREA)

DK01117785T 2000-08-29 2001-08-02 Fremgangsmåde til fremstilling af humane bruskimplantater ved hjælp af in vitro dyrkede chondrocytter DK1201749T3 (da)

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
DE10042484A DE10042484A1 (de)	2000-08-29	2000-08-29	Verfahren zur Herstellung von humanen Knorpelimplantaten mittels in vitro gezüchteter Chondrozyten

Publications (1)

Publication Number	Publication Date
DK1201749T3 true DK1201749T3 (da)	2008-01-07

Family

ID=7654235

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
DK01117785T DK1201749T3 (da)	2000-08-29	2001-08-02	Fremgangsmåde til fremstilling af humane bruskimplantater ved hjælp af in vitro dyrkede chondrocytter

Country Status (10)

Country	Link
US (1)	US6730314B2 (da)
EP (1)	EP1201749B1 (da)
JP (1)	JP2002078484A (da)
AT (1)	ATE370227T1 (da)
CA (1)	CA2356024C (da)
CY (1)	CY1107771T1 (da)
DE (2)	DE10042484A1 (da)
DK (1)	DK1201749T3 (da)
ES (1)	ES2291253T3 (da)
PT (1)	PT1201749E (da)

Families Citing this family (45)

* Cited by examiner, † Cited by third party
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WO2003050273A1 (fr) *	2001-12-13	2003-06-19	Japan Science And Technology Agency	Milieu de culture pour des cellules humaines et methode de culture
JP2005516972A (ja) *	2002-01-22	2005-06-09	ファイザー・インク	血栓疾患の治療のためのｔａｆｉ−ａ阻害剤として使用される３−（イミダゾリル）−２−アミノプロピオン酸
US7931687B2 (en) *	2002-05-13	2011-04-26	Articular Engineering, Llc	Tissue engineered osteochondral implant
US20060134158A1 (en) *	2002-06-28	2006-06-22	Chemical Biology Institute	Chitosan/acidic biopolymer hybrid fiber and culture base for animal cells
US7067123B2 (en)	2003-04-29	2006-06-27	Musculoskeletal Transplant Foundation	Glue for cartilage repair
US7901457B2 (en)	2003-05-16	2011-03-08	Musculoskeletal Transplant Foundation	Cartilage allograft plug
US7488348B2 (en) *	2003-05-16	2009-02-10	Musculoskeletal Transplant Foundation	Cartilage allograft plug
EP1649875B1 (en) *	2003-07-31	2011-10-12	Iwamoto, Yukihide	Method of constructing artificial joint
DE602004018534D1 (de) *	2003-10-10	2009-01-29	Kw2 Implantattechnologie Gmbh	Knorpelregeneration mit generation von chondronen unter hoher konzentration von magnesium
US8697139B2 (en)	2004-09-21	2014-04-15	Frank M. Phillips	Method of intervertebral disc treatment using articular chondrocyte cells
US7837740B2 (en)	2007-01-24	2010-11-23	Musculoskeletal Transplant Foundation	Two piece cancellous construct for cartilage repair
US7700355B2 (en) *	2005-01-07	2010-04-20	Industrial Technology Research Institute	Methods of producing a porous matrix for culturing and recovering cells
US9074175B2 (en)	2005-02-07	2015-07-07	Nb Health Laboratory Co., Ltd.	Method of regenerating elastic fiber with the use of dance or factor enhancing the expression thereof
ES2693609T3 (es) *	2005-02-28	2018-12-12	Cellseed Inc.	Lámina de células cultivadas, método para su producción y método para su aplicación
DE102005030614B4 (de)	2005-06-30	2014-05-08	Biotissue Ag	Zellfreies Transplantat, dessen Verwendung, Verfahren zu dessen Herstellung, dabei hergestellte Matrix mit Gel und Verfahren zur Herstellung dieser Matrix mit Gel
US7815926B2 (en)	2005-07-11	2010-10-19	Musculoskeletal Transplant Foundation	Implant for articular cartilage repair
JP4044579B2 (ja)	2005-08-02	2008-02-06	株式会社Ｐｇリサーチ	人工軟骨組織
US8921109B2 (en)	2005-09-19	2014-12-30	Histogenics Corporation	Cell-support matrix having narrowly defined uniformly vertically and non-randomly organized porosity and pore density and a method for preparation thereof
EP1764117A1 (en)	2005-09-20	2007-03-21	Zimmer GmbH	Implant for the repair of a cartilage defect and method for manufacturing the implant
US8293225B2 (en)	2005-10-24	2012-10-23	Ed. Geistlich Soehne Ag Fuer Chemische Industrie	Method and device for synovial cell-charged collagen membrane or gel
DE102006047346A1 (de)	2006-10-06	2008-04-10	Transtissue Technologies Gmbh	Matrix-Gel-Transplantat ohne Zellen
US20080241250A1 (en) *	2006-11-08	2008-10-02	Emans Pieter J	In vivo bioreactors and methods of making and using same
WO2008064132A2 (en) *	2006-11-17	2008-05-29	Trustees Of Dartmouth College	Synthetic triterpenoids and tricyclic-bis-enones for use in stimulating bone and cartilage growth
US8435551B2 (en)	2007-03-06	2013-05-07	Musculoskeletal Transplant Foundation	Cancellous construct with support ring for repair of osteochondral defects
JP4739286B2 (ja) *	2007-07-17	2011-08-03	株式会社Ｐｇリサーチ	人工軟骨組織の製造方法
CA2717725A1 (en)	2008-03-05	2009-09-11	Musculoskeletal Transplant Foundation	Cancellous constructs, cartilage particles and combinations of cancellous constructs and cartilage particles
CA2744445C (en) *	2008-11-25	2023-10-24	Tissuegene, Inc.	Primed cell therapy
US20120045419A1 (en) *	2009-02-06	2012-02-23	University Health Network	Methods and uses of hypoxic compartment cells
EP2625264B1 (en)	2010-10-08	2022-12-07	Terumo BCT, Inc.	Methods and systems of growing and harvesting cells in a hollow fiber bioreactor system with control conditions
EP2882465B1 (en) *	2012-08-08	2019-05-08	Nanyang Technological University	Methods of manufacturing hydrogel microparticles having living cells, and compositions for manufacturing a scaffold for tissue engineering
EP3068867B1 (en)	2013-11-16	2018-04-18	Terumo BCT, Inc.	Expanding cells in a bioreactor
JP6783143B2 (ja)	2014-03-25	2020-11-11	テルモビーシーティー、インコーポレーテッド	培地の受動的補充
WO2016049421A1 (en)	2014-09-26	2016-03-31	Terumo Bct, Inc.	Scheduled feed
US10077420B2 (en)	2014-12-02	2018-09-18	Histogenics Corporation	Cell and tissue culture container
WO2017004592A1 (en)	2015-07-02	2017-01-05	Terumo Bct, Inc.	Cell growth with mechanical stimuli
WO2017205667A1 (en)	2016-05-25	2017-11-30	Terumo Bct, Inc.	Cell expansion
US11104874B2 (en)	2016-06-07	2021-08-31	Terumo Bct, Inc.	Coating a bioreactor
US11685883B2 (en)	2016-06-07	2023-06-27	Terumo Bct, Inc.	Methods and systems for coating a cell growth surface
US11624046B2 (en)	2017-03-31	2023-04-11	Terumo Bct, Inc.	Cell expansion
US12234441B2 (en)	2017-03-31	2025-02-25	Terumo Bct, Inc.	Cell expansion
EP3656841A1 (en)	2017-03-31	2020-05-27	Terumo BCT, Inc.	Cell expansion
CN107854730A (zh) *	2017-10-31	2018-03-30	暨南大学	交联cga的海藻酸钠‑明胶共混膜关节补片的制备方法及其应用
PL242834B1 (pl) *	2018-04-10	2023-05-02	Julia Bar	Sposób określania zagęszczenia chondrocytów w biologicznym implancie do regeneracji tkanki chrzęstnej
JP2024511064A (ja)	2021-03-23	2024-03-12	テルモビーシーティー、インコーポレーテッド	細胞捕獲及び増殖
US12209689B2 (en)	2022-02-28	2025-01-28	Terumo Kabushiki Kaisha	Multiple-tube pinch valve assembly

2000
- 2000-08-29 DE DE10042484A patent/DE10042484A1/de not_active Withdrawn
2001
- 2001-08-02 ES ES01117785T patent/ES2291253T3/es not_active Expired - Lifetime
- 2001-08-02 EP EP01117785A patent/EP1201749B1/de not_active Expired - Lifetime
- 2001-08-02 PT PT01117785T patent/PT1201749E/pt unknown
- 2001-08-02 DE DE50112855T patent/DE50112855D1/de not_active Expired - Lifetime
- 2001-08-02 DK DK01117785T patent/DK1201749T3/da active
- 2001-08-02 AT AT01117785T patent/ATE370227T1/de active
- 2001-08-27 JP JP2001255410A patent/JP2002078484A/ja active Pending
- 2001-08-27 CA CA2356024A patent/CA2356024C/en not_active Expired - Fee Related
- 2001-08-29 US US09/940,854 patent/US6730314B2/en not_active Expired - Fee Related
2007
- 2007-10-22 CY CY20071101353T patent/CY1107771T1/el unknown

Also Published As

Publication number	Publication date
JP2002078484A (ja)	2002-03-19
CY1107771T1 (el)	2013-04-18
CA2356024A1 (en)	2002-02-28
DE10042484A1 (de)	2002-03-28
ES2291253T3 (es)	2008-03-01
ATE370227T1 (de)	2007-09-15
US6730314B2 (en)	2004-05-04
EP1201749B1 (de)	2007-08-15
US20020052044A1 (en)	2002-05-02
DE50112855D1 (de)	2007-09-27
EP1201749A1 (de)	2002-05-02
PT1201749E (pt)	2007-11-14
CA2356024C (en)	2011-08-09

Publication	Publication Date	Title
DK1201749T3 (da)	2008-01-07	Fremgangsmåde til fremstilling af humane bruskimplantater ved hjælp af in vitro dyrkede chondrocytter
SG144689A1 (en)	2008-08-28	Embryonic stem cells and neural progenitor cells derived therefrom
DE60238416D1 (de)	2011-01-05	Plasmaprotein-matritzes und verfahren zu ihrer herstellung
ATE332964T1 (de)	2006-08-15	Mittels gewebetechnologie hergestellte dezellularisierte gegenstände und gewebe
CY1106821T1 (el)	2012-05-23	Μεθοδος για την καλλιεργεια υποκαταστατου χονδρου και βιολογικη μητρα, παραγομενη συμφωνα με τη μεθοδο αυτη
MX2009014273A (es)	2010-04-01	Metodos y composiciones para expansion e implantacion optimizadas de celulas madre mesenquimales.
NZ569543A (en)	2012-12-21	Means and methods for influencing the stability of antibody producing cells
WO2009137624A3 (en)	2010-01-07	Hemangio colony forming cells and non-engrafting hemangio cells
MXPA05009096A (es)	2005-10-19	Metodo de cultivo celular libre de animal.
WO2003014313A3 (en)	2003-04-24	Alternative compositions and methods for the culture of stem cells
MX2012000455A (es)	2012-04-11	Composiciones de matriz extra-celular y medio acondicionado a partir de celulas cultivadas bajo condiciones hipoxicas.
MXPA04009031A (es)	2005-12-12	Celulas madre hepaticas primitivas y proximales.
BR0316757A (pt)	2005-10-25	Composições de meio de cultura de célula, processo para a cultura de célula progenitoras e/ou tronco, processo para a produção de mioblastos, população de células, e uso de mioblastos
DE60041821D1 (de)	2009-04-30	Pluripotent embryonale stammzellen-ähnliche zellen, zusammensetzungen und ihre vervendungen
Zainal Ariffin et al.	2012	In vitro chondrogenesis transformation study of mouse dental pulp stem cells
ATE553186T1 (de)	2012-04-15	Bandscheibenreparatur und verfahren dafür
GB2428044A (en)	2007-01-17	Method of forming mesenchymal stem cells from embryonic stem cells
MXPA04012601A (es)	2005-09-30	Medio de cultivo de celulas madre-progenitoras autologas humanas y sus aplicaciones.
CA2543118A1 (en)	2005-05-06	Method for differentiating mesenchymal stem cell into neural cell and pharmaceutical composition containing the neural cell for neurodegenerative disease
WO2004043401A3 (en)	2004-09-30	Method of preparing anti-angiogenic drug from cartillage and chondrocytes and methods of use
BR0014671A (pt)	2002-07-23	Método para a produção de substâncias proteináceas
ATE500318T1 (de)	2011-03-15	Gerüstlose konstrukte für die erzeugung von gelenkknorpel aus gewebe
FR2948381B1 (fr)	2013-07-12	Procede d'obtention de myofibroblastes.
ATE414141T1 (de)	2008-11-15	Verfahren zur kultivierung humaner chondrozyten
Bryja et al.	2019	Overview of the different methods used in the primary culture of oral mucosa cells