[go: up one dir, main page]

DK0994962T3 - Et indekseret bibliotek af celler indeholdende genomiske modifikationer og fremgangsmåder til at fremstille og anvende samme - Google Patents

Et indekseret bibliotek af celler indeholdende genomiske modifikationer og fremgangsmåder til at fremstille og anvende samme

Info

Publication number
DK0994962T3
DK0994962T3 DK97910766T DK97910766T DK0994962T3 DK 0994962 T3 DK0994962 T3 DK 0994962T3 DK 97910766 T DK97910766 T DK 97910766T DK 97910766 T DK97910766 T DK 97910766T DK 0994962 T3 DK0994962 T3 DK 0994962T3
Authority
DK
Denmark
Prior art keywords
library
methods
cells
same
vectors
Prior art date
Application number
DK97910766T
Other languages
English (en)
Inventor
Arthur Sands
Glenn Friedrich
Brian Zambrowicz
Allan Bradley
Original Assignee
Lexicon Pharmaceuticals Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US08/726,867 external-priority patent/US6136566A/en
Priority claimed from US08/907,598 external-priority patent/US6139833A/en
Application filed by Lexicon Pharmaceuticals Inc filed Critical Lexicon Pharmaceuticals Inc
Application granted granted Critical
Publication of DK0994962T3 publication Critical patent/DK0994962T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1082—Preparation or screening gene libraries by chromosomal integration of polynucleotide sequences, HR-, site-specific-recombination, transposons, viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1051—Gene trapping, e.g. exon-, intron-, IRES-, signal sequence-trap cloning, trap vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/07—Animals genetically altered by homologous recombination
    • A01K2217/075—Animals genetically altered by homologous recombination inducing loss of function, i.e. knock out
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/10041—Use of virus, viral particle or viral elements as a vector
    • C12N2740/10043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/60—Vectors containing traps for, e.g. exons, promoters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
    • C12N2840/203—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/44—Vectors comprising a special translation-regulating system being a specific part of the splice mechanism, e.g. donor, acceptor

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
DK97910766T 1996-10-04 1997-10-03 Et indekseret bibliotek af celler indeholdende genomiske modifikationer og fremgangsmåder til at fremstille og anvende samme DK0994962T3 (da)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US08/726,867 US6136566A (en) 1996-10-04 1996-10-04 Indexed library of cells containing genomic modifications and methods of making and utilizing the same
US72896396A 1996-10-11 1996-10-11
US08/907,598 US6139833A (en) 1997-08-08 1997-08-08 Targeted gene discovery
PCT/US1997/017791 WO1998014614A1 (en) 1996-10-04 1997-10-03 An indexed library of cells containing genomic modifications and methods of making and utilizing the same

Publications (1)

Publication Number Publication Date
DK0994962T3 true DK0994962T3 (da) 2009-04-20

Family

ID=27419091

Family Applications (1)

Application Number Title Priority Date Filing Date
DK97910766T DK0994962T3 (da) 1996-10-04 1997-10-03 Et indekseret bibliotek af celler indeholdende genomiske modifikationer og fremgangsmåder til at fremstille og anvende samme

Country Status (9)

Country Link
US (2) US6207371B1 (da)
EP (1) EP0994962B1 (da)
JP (1) JP2002503083A (da)
AT (1) ATE419381T1 (da)
AU (1) AU744030B2 (da)
CA (1) CA2268363C (da)
DE (1) DE69739192D1 (da)
DK (1) DK0994962T3 (da)
WO (1) WO1998014614A1 (da)

Families Citing this family (54)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7332338B2 (en) 1996-10-04 2008-02-19 Lexicon Pharmaceuticals, Inc. Vectors for making genomic modifications
US6855545B1 (en) * 1996-10-04 2005-02-15 Lexicon Genetics Inc. Indexed library of cells containing genomic modifications and methods of making and utilizing the same
US20040072243A1 (en) * 1996-10-11 2004-04-15 Lexicon Genetics Incorporated Indexed library of cells containing genomic modifications and methods of making and utilizing the same
US6228639B1 (en) 1996-12-31 2001-05-08 George A. Gaitanaris Vectors and methods for the mutagenesis of mammalian genes
CA2205888A1 (en) * 1997-07-11 1999-01-11 John J. Priatel Complementation trap
US6897066B1 (en) 1997-09-26 2005-05-24 Athersys, Inc. Compositions and methods for non-targeted activation of endogenous genes
US6740503B1 (en) 1997-09-26 2004-05-25 Athersys, Inc. Compositions and methods for non-targeted activation of endogenous genes
WO1999050426A1 (en) * 1998-03-27 1999-10-07 Lexicon Genetics Incorporated Vectors for gene mutagenesis and gene discovery
US6808921B1 (en) * 1998-03-27 2004-10-26 Lexicon Genetics Incorporated Vectors for gene mutagenesis and gene discovery
US6436707B1 (en) * 1998-03-27 2002-08-20 Lexicon Genetics Incorporated Vectors for gene mutagenesis and gene discovery
JP2002522071A (ja) 1998-08-10 2002-07-23 レキシコン ジェネティックス インコーポレーテッド 真核細胞由来の平均化ｃＤＮＡライブラリーの構築
US20070178475A1 (en) * 1998-09-17 2007-08-02 Nehls Michael C Novel human polynucleotides and polypeptides encoded thereby
FR2786787B1 (fr) * 1998-12-08 2002-04-19 Proteus Methode d'analyse in vitro d'un phenotype connu a partir d'un echantillon d'acides nucleiques
AR022204A1 (es) * 1999-01-08 2002-09-04 Norgine Bv Compuesto, proceso para su preparacion, composicion farmaceutica y producto comestible que lo comprende.
DE60043985D1 (de) 1999-07-14 2010-04-22 Transgenic Inc "gen-fallen-vektor" und verfahren zum "einfangen" von genen mittels dieses vektors
US6716614B1 (en) 1999-09-02 2004-04-06 Lexicon Genetics Incorporated Human calcium dependent proteases, polynucleotides encoding the same, and uses thereof
US6541252B1 (en) 2000-05-19 2003-04-01 Lexicon Genetics Incorporated Human kinases and polynucleotides encoding the same
GB0000908D0 (en) * 2000-01-14 2000-03-08 Scient Generics Ltd Parallel free-space optical communications
AU2001249267A1 (en) * 2000-03-20 2001-10-03 James N. Higginbotham Methods and compositions for elucidating protein expression profiles in cells
US6897020B2 (en) 2000-03-20 2005-05-24 Newlink Genetics Inc. Methods and compositions for elucidating relative protein expression levels in cells
US6716582B2 (en) * 2000-04-14 2004-04-06 E. I. Du Pont De Nemours And Company Cellular arrays for the identification of altered gene expression
FR2810048A1 (fr) * 2000-06-07 2001-12-14 Centre Nat Rech Scient Construction d'acide nucleique utile pour l'expression de transgenes notamment dans les cellules souches embryonnaires
CA2309371A1 (en) 2000-06-16 2001-12-16 Christopher J. Ong Gene sequence tag method
US20060031947A1 (en) * 2001-07-25 2006-02-09 Alejandro Abuin Novel mutated mammalian cells and animals
US20020031829A1 (en) * 2000-08-15 2002-03-14 Brian Zambrowicz Arrayed collection of genomic clones
US7001763B1 (en) 2000-10-17 2006-02-21 Lexicon Genetics Incorporated Human semaphorin proteins and polynucleotides encoding the same
JP3929250B2 (ja) * 2001-03-08 2007-06-13 株式会社ルネサステクノロジ 半導体装置
WO2002090498A2 (en) * 2001-05-04 2002-11-14 Health Research, Inc. A high throughput assay for identification of gene expression modifiers
US20030143578A1 (en) * 2001-08-24 2003-07-31 Pruitt Steven C. High throughput method for identification of sequence tags
JP2005500847A (ja) * 2001-08-24 2005-01-13 アドバンスド セル テクノロジー、インコーポレイテッド 分化誘導薬の同定のためのスクリーニング検定及び細胞治療用の分化細胞の調製
EP1295951A1 (en) * 2001-09-24 2003-03-26 The University of British Columbia Cell library method
WO2003027264A2 (en) * 2001-09-27 2003-04-03 University Of Delaware Coisogenic eukaryotic cell collections
AU2003231244A1 (en) * 2002-05-02 2003-11-17 University Of North Carolina At Chapel Hill In vitro mutagenesis, phenotyping, and gene mapping
US20040091936A1 (en) 2002-05-24 2004-05-13 Michael West Bank of stem cells for producing cells for transplantation having HLA antigens matching those of transplant recipients, and methods for making and using such a stem cell bank
WO2004070002A2 (en) * 2002-11-18 2004-08-19 The Government Of The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services, Centers For Disease Control And Prevention Cell lines and host nucleic acid sequences related to infectious disease
AU2004206256A1 (en) * 2003-01-16 2004-08-05 Health Research, Inc. Method for comprehensive identification of cell lineage specific genes
RU2006114432A (ru) * 2003-09-30 2007-11-20 Лексикон Дженетикс Инкорпорейтед (Us) Способы и композиции для определения функции гена
KR20070007793A (ko) * 2004-02-05 2007-01-16 메사추세츠 인스티튜트 오브 테크놀로지 세포 디스플레이 라이브러리
CA2465396A1 (en) * 2004-04-20 2005-10-20 Shawn Burgess Rapid integration site mapping
US20090221440A1 (en) * 2004-07-12 2009-09-03 Board Of Regents, The University Of Texas System Methods and compositions related to identifying protein-protein interactions
US20060024819A1 (en) * 2004-07-30 2006-02-02 Finney Robert E Integration vectors
WO2006128149A1 (en) * 2005-05-26 2006-11-30 Lexicon Genetics Incorporated Methods and compositions for defining gene function
US7649098B2 (en) 2006-02-24 2010-01-19 Lexicon Pharmaceuticals, Inc. Imidazole-based compounds, compositions comprising them and methods of their use
US20100070186A1 (en) * 2006-03-15 2010-03-18 Soper Bryan R Methods of screening for and mapping phenotypic and genotypic variations in cells
US20080004410A1 (en) * 2006-06-30 2008-01-03 Yu-Chin Lai Hydrophilic macromonomers having alpha,beta-conjugated carboxylic terminal group and medical devices incorporating same
JP4867012B2 (ja) * 2007-03-22 2012-02-01 国立大学法人群馬大学 レトロウイルス産生用ベクター
WO2013169957A1 (en) 2012-05-08 2013-11-14 Adaptive Biotechnologies Corporation Compositions and method for measuring and calibrating amplification bias in multiplexed pcr reactions
EP2978849A2 (en) * 2013-03-30 2016-02-03 Usha Biotech Limited Methods and constructs for expressing biologically active proteins in mammalian cells
US9708657B2 (en) 2013-07-01 2017-07-18 Adaptive Biotechnologies Corp. Method for generating clonotype profiles using sequence tags
US11390921B2 (en) 2014-04-01 2022-07-19 Adaptive Biotechnologies Corporation Determining WT-1 specific T cells and WT-1 specific T cell receptors (TCRs)
US10066265B2 (en) 2014-04-01 2018-09-04 Adaptive Biotechnologies Corp. Determining antigen-specific t-cells
ES2784343T3 (es) 2014-10-29 2020-09-24 Adaptive Biotechnologies Corp Detección simultánea altamente multiplexada de ácidos nucleicos que codifican heterodímeros de receptores inmunes adaptativos emparejados de muchas muestras
US10428325B1 (en) 2016-09-21 2019-10-01 Adaptive Biotechnologies Corporation Identification of antigen-specific B cell receptors
US11254980B1 (en) 2017-11-29 2022-02-22 Adaptive Biotechnologies Corporation Methods of profiling targeted polynucleotides while mitigating sequencing depth requirements

Family Cites Families (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4109496A (en) 1977-12-20 1978-08-29 Norris Industries Trapped key mechanism
US4487829A (en) * 1982-03-23 1984-12-11 Massachusetts Institute Of Technology Production and use of monoclonal antibodies against adenoviruses
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4800159A (en) 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
SE8602936D0 (sv) 1986-07-02 1986-07-02 Fisheries Manag Supply Ab Utmatningsanordning
US5079352A (en) 1986-08-22 1992-01-07 Cetus Corporation Purified thermostable enzyme
US4889818A (en) 1986-08-22 1989-12-26 Cetus Corporation Purified thermostable enzyme
WO1988001646A1 (en) * 1986-08-26 1988-03-10 Allelix Inc. Universal system for transposon mutagenesis
JP3082204B2 (ja) 1988-09-01 2000-08-28 ホワイトヘッド・インスティチュート・フォー・バイオメディカル・リサーチ 両栄養性および環境栄養性宿主域を持つ組換え体レトロウイルス
US5091310A (en) 1988-09-23 1992-02-25 Cetus Corporation Structure-independent dna amplification by the polymerase chain reaction
US5075216A (en) 1988-09-23 1991-12-24 Cetus Corporation Methods for dna sequencing with thermus aquaticus dna polymerase
US5066584A (en) 1988-09-23 1991-11-19 Cetus Corporation Methods for generating single stranded dna by the polymerase chain reaction
US5023171A (en) 1989-08-10 1991-06-11 Mayo Foundation For Medical Education And Research Method for gene splicing by overlap extension using the polymerase chain reaction
US5464764A (en) 1989-08-22 1995-11-07 University Of Utah Research Foundation Positive-negative selection methods and vectors
US5104792A (en) 1989-12-21 1992-04-14 The United States Of America As Represented By The Department Of Health And Human Services Method for amplifying unknown nucleic acid sequences
US5364783A (en) * 1990-05-14 1994-11-15 Massachusetts Institute Of Technology Retrovirus promoter-trap vectors
US5801030A (en) 1995-09-01 1998-09-01 Genvec, Inc. Methods and vectors for site-specific recombination
US5928888A (en) * 1996-09-26 1999-07-27 Aurora Biosciences Corporation Methods and compositions for sensitive and rapid, functional identification of genomic polynucleotides and secondary screening capabilities
CA2271228A1 (en) 1996-11-08 1998-05-14 Jonathan W. Jarvik Method for producing tagged genes, transcripts and proteins
DE19650714A1 (de) 1996-12-06 1998-06-10 Melchner Harald Von Prof Dr Genfallen-Konstrukt zur Identifizierung und Isolierung von Genen

Also Published As

Publication number Publication date
EP0994962A1 (en) 2000-04-26
DE69739192D1 (de) 2009-02-12
JP2002503083A (ja) 2002-01-29
CA2268363A1 (en) 1998-04-09
EP0994962B1 (en) 2008-12-31
CA2268363C (en) 2012-07-10
AU744030B2 (en) 2002-02-14
EP0994962A4 (en) 2005-03-16
US20090149336A1 (en) 2009-06-11
AU4805697A (en) 1998-04-24
ATE419381T1 (de) 2009-01-15
WO1998014614A1 (en) 1998-04-09
US6207371B1 (en) 2001-03-27

Similar Documents

Publication Publication Date Title
DK0994962T3 (da) Et indekseret bibliotek af celler indeholdende genomiske modifikationer og fremgangsmåder til at fremstille og anvende samme
Sigmon et al. Content and performance of the MiniMUGA genotyping array: a new tool to improve rigor and reproducibility in mouse research
Rougeux et al. Modeling the multiple facets of speciation-with-gene-flow toward inferring the divergence history of lake whitefish species pairs (Coregonus clupeaformis)
Auwerx et al. The European dimension for the mouse genome mutagenesis program
Brandrud et al. Phylogenomic relationships of diploids and the origins of allotetraploids in Dactylorhiza (Orchidaceae)
Wu et al. Physical maps and recombination frequency of six rice chromosomes
Murat et al. Understanding Brassicaceae evolution through ancestral genome reconstruction
Elbers et al. Improving Illumina assemblies with Hi‐C and long reads: An example with the North African dromedary
Justice et al. Mouse ENU mutagenesis
Glöckner et al. Sequence and analysis of chromosome 2 of Dictyostelium discoideum
ES2229781T3 (es) Metodos programas y aparatos para identificar regiones genomicas que albergan un gen asociado con un rasgo detectable.
Nakazato et al. Targeted base editing in the mitochondrial genome of Arabidopsis thaliana
Singhal et al. Squamate conserved loci (Sq CL): a unified set of conserved loci for phylogenomics and population genetics of squamate reptiles
Hämälä et al. Biased gene conversion constrains adaptation in Arabidopsis thaliana
Peng et al. Forward-time population genetics simulations: methods, implementation, and applications
WO2000046406A3 (en) Arrays for investigating protein protein interactions
De Ronne et al. 3D-GBS: A universal genotyping-by-sequencing approach for genomic selection and other high-throughput low-cost applications in species with small to medium-sized genomes
Jackson et al. Mouse genetics and transgenics: a practical approach
Christiansen Theories of population variation in genes and genomes
Luo et al. Strand-biased DNA methylation associated with centromeric regions in Arabidopsis
Lim et al. Telomeric repeat evolution in the phylum Nematoda revealed by high-quality genome assemblies and subtelomere structures
Schimenti et al. Interdigitated deletion complexes on mouse chromosome 5 induced by irradiation of embryonic stem cells
Yu et al. Precise age estimation in clonal species using a somatic genetic clock
Brickner et al. Identification, mapping and linkage analysis of randomly amplified DNA polymorphisms in Tetrahymena thermophila
Paterson Prospects for cloning the genetic determinants of QTLs