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DK0410895T3 - Process for producing protein from Bacillus subtilis strains and method for producing these strains - Google Patents

Process for producing protein from Bacillus subtilis strains and method for producing these strains

Info

Publication number
DK0410895T3
DK0410895T3 DK90402172.2T DK90402172T DK0410895T3 DK 0410895 T3 DK0410895 T3 DK 0410895T3 DK 90402172 T DK90402172 T DK 90402172T DK 0410895 T3 DK0410895 T3 DK 0410895T3
Authority
DK
Denmark
Prior art keywords
gene
protein
induced
strain
expression
Prior art date
Application number
DK90402172.2T
Other languages
Danish (da)
Inventor
Georges Rapoport
Gwennael Joliff
Marie-Agnes Petit
Andre Klier
Dusko Stanislas Ehrlich
Phillipe Looten
Didier Vernade
Juan Manuel Mesas
Original Assignee
Agronomique Inst Nat Rech
Pasteur Institut
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Agronomique Inst Nat Rech, Pasteur Institut filed Critical Agronomique Inst Nat Rech
Application granted granted Critical
Publication of DK0410895T3 publication Critical patent/DK0410895T3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01004—Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/07—Bacillus
    • C12R2001/125—Bacillus subtilis ; Hay bacillus; Grass bacillus

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Process for the production of a specified protein, characterised in that the following operations are carried out starting with a strain of the genus Bacillus in the chromosome of which the following have been incorporated into a nonessential gene of the said strain. a) an amplifiable unit comprising a specified DNA sequence M framed by two identical DNA sequences D oriented in direct direction, the sequence M comprising the gene for the structure of the said protein placed under the control of expression and secretion elements capable of being induced into Bacillus of the said protein and optionally a selectable gene, and b) a replication system or replicon capable of being induced or conditional also incorporated into the said nonessential gene of the chromosome of the strain upstream of the amplifiable unit; 1. the amplified clones obtained by culture on a selection medium corresponding to the selectable gene are optionally selected and the strains whose genome corresponds to the presence of a number of copies of the said gene which is enhanced in relation to the bacterial population before selection are abstracted; and 2. a gene amplification is induced or conditioned in an appropriate culture medium by the activation of the replicon and the expression and the secretion of the protein and the activation of the said expression elements are induced, 3. the said expressed protein is recovered. The invention also relates to a process for the preparation of a Bacillus strain, amplified or otherwise, employed especially in the production process according to the invention.
DK90402172.2T 1989-07-27 1990-07-27 Process for producing protein from Bacillus subtilis strains and method for producing these strains DK0410895T3 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR898910143A FR2650292B1 (en) 1989-07-27 1989-07-27 PROCESS FOR PRODUCING PROTEIN FROM SUBSTRATE BACILLUS STRAINS AND PROCESS FOR PREPARING SAID STRAINS

Publications (1)

Publication Number Publication Date
DK0410895T3 true DK0410895T3 (en) 1994-10-03

Family

ID=9384203

Family Applications (1)

Application Number Title Priority Date Filing Date
DK90402172.2T DK0410895T3 (en) 1989-07-27 1990-07-27 Process for producing protein from Bacillus subtilis strains and method for producing these strains

Country Status (6)

Country Link
EP (1) EP0410895B1 (en)
AT (1) ATE105866T1 (en)
DE (1) DE69008959T2 (en)
DK (1) DK0410895T3 (en)
ES (1) ES2056410T3 (en)
FR (1) FR2650292B1 (en)

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2563533B1 (en) * 1984-04-27 1986-08-22 Centre Nat Rech Scient METHOD FOR AMPLIFYING THE EXPRESSION OF A DETERMINED GENE IN SUBACTIVE BACILLUS AND STRAINS OBTAINED
FR2582316B1 (en) * 1985-05-22 1988-12-02 Centre Nat Rech Scient VECTORS FOR THE EXPRESSION OF A-AMYLASE IN SUBTIL BACILLUS, STRAINS OBTAINED AND PROCESS FOR THE PREPARATION OF A-AMYLASE

Also Published As

Publication number Publication date
DE69008959T2 (en) 1994-12-22
FR2650292A1 (en) 1991-02-01
ATE105866T1 (en) 1994-06-15
ES2056410T3 (en) 1994-10-01
EP0410895A1 (en) 1991-01-30
DE69008959D1 (en) 1994-06-23
FR2650292B1 (en) 1994-03-04
EP0410895B1 (en) 1994-05-18

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