DE746534C - Utilization of yeast waste water - Google Patents
Utilization of yeast waste waterInfo
- Publication number
- DE746534C DE746534C DEP83422D DEP0083422D DE746534C DE 746534 C DE746534 C DE 746534C DE P83422 D DEP83422 D DE P83422D DE P0083422 D DEP0083422 D DE P0083422D DE 746534 C DE746534 C DE 746534C
- Authority
- DE
- Germany
- Prior art keywords
- yeast
- waste water
- hydrolysis
- wastewater
- utilization
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 240000004808 Saccharomyces cerevisiae Species 0.000 title claims description 24
- 239000002351 wastewater Substances 0.000 title claims description 15
- 230000007062 hydrolysis Effects 0.000 claims description 10
- 238000006460 hydrolysis reaction Methods 0.000 claims description 10
- 238000000034 method Methods 0.000 claims description 8
- 239000002994 raw material Substances 0.000 claims description 6
- 239000002253 acid Substances 0.000 claims description 5
- 239000001913 cellulose Substances 0.000 description 7
- 229920002678 cellulose Polymers 0.000 description 7
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 6
- 238000000855 fermentation Methods 0.000 description 6
- 230000004151 fermentation Effects 0.000 description 6
- 239000000126 substance Substances 0.000 description 5
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 4
- 230000002378 acidificating effect Effects 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 239000010902 straw Substances 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- 239000002023 wood Substances 0.000 description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- 238000010306 acid treatment Methods 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 150000003839 salts Chemical class 0.000 description 3
- 210000005253 yeast cell Anatomy 0.000 description 3
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 2
- 229910019142 PO4 Inorganic materials 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- 238000004140 cleaning Methods 0.000 description 2
- 230000002401 inhibitory effect Effects 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 235000021317 phosphate Nutrition 0.000 description 2
- 150000003013 phosphoric acid derivatives Chemical class 0.000 description 2
- 235000001674 Agaricus brunnescens Nutrition 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 240000000731 Fagus sylvatica Species 0.000 description 1
- 235000010099 Fagus sylvatica Nutrition 0.000 description 1
- 108010058643 Fungal Proteins Proteins 0.000 description 1
- 241000218657 Picea Species 0.000 description 1
- 241000209504 Poaceae Species 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 244000061456 Solanum tuberosum Species 0.000 description 1
- 235000002595 Solanum tuberosum Nutrition 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 1
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 1
- 235000011130 ammonium sulphate Nutrition 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 239000000920 calcium hydroxide Substances 0.000 description 1
- 235000011116 calcium hydroxide Nutrition 0.000 description 1
- OSGAYBCDTDRGGQ-UHFFFAOYSA-L calcium sulfate Inorganic materials [Ca+2].[O-]S([O-])(=O)=O OSGAYBCDTDRGGQ-UHFFFAOYSA-L 0.000 description 1
- ZOMBKNNSYQHRCA-UHFFFAOYSA-J calcium sulfate hemihydrate Chemical compound O.[Ca+2].[Ca+2].[O-]S([O-])(=O)=O.[O-]S([O-])(=O)=O ZOMBKNNSYQHRCA-UHFFFAOYSA-J 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 239000000084 colloidal system Substances 0.000 description 1
- 238000010411 cooking Methods 0.000 description 1
- 239000003085 diluting agent Substances 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 229910052602 gypsum Inorganic materials 0.000 description 1
- 239000010440 gypsum Substances 0.000 description 1
- 239000011507 gypsum plaster Substances 0.000 description 1
- 230000003301 hydrolyzing effect Effects 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- 235000019341 magnesium sulphate Nutrition 0.000 description 1
- 150000007522 mineralic acids Chemical class 0.000 description 1
- 150000002829 nitrogen Chemical class 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 235000005985 organic acids Nutrition 0.000 description 1
- 230000036284 oxygen consumption Effects 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052939 potassium sulfate Inorganic materials 0.000 description 1
- 235000011151 potassium sulphates Nutrition 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 238000002203 pretreatment Methods 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 239000010865 sewage Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 239000012209 synthetic fiber Substances 0.000 description 1
- 229920002994 synthetic fiber Polymers 0.000 description 1
- 239000003643 water by type Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/22—Processes using, or culture media containing, cellulose or hydrolysates thereof
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Tropical Medicine & Parasitology (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Description
Ausnutzung von Hefeabwässern Neben .der Hefe der Brennereien und der Brauereihefe erhält in neuester Zeit die aus den durch Säurebehandlung.cellulosehaltiger Rohstoffe gewonnenen Zuckerlösungen hergestellte Hefe steigende Bedeutung. Insbesondere werden :auch die beider Säurebehandlung solcher cellulosehaltiger Rohstoffe, wie Holz oder Stroh, zwecks Herstellung eines Zellstoffes von hohem Allphacellulosegehalt für die Kunstfaserindustrie anfallenden Lösungen für die Hefegewinnung verarbeitet. Die Begleitstoffe der Cellulose, die Pentosane, werden durch die Säurebehandlung in Zucker übergeführt und-können auf diese Weise nutzbringend verwertet werden.Utilization of yeast wastewater In addition to the yeast of the distilleries and the Brewery yeast has recently been obtained from the cellulose-containing cells through acid treatment Raw materials obtained from sugar solutions, yeast produced increasing importance. In particular are: also the acid treatment of such cellulose-containing raw materials, such as Wood or straw, for the purpose of producing a pulp with a high allphacellulose content For the synthetic fiber industry, we process solutions for yeast extraction. The accompanying substances of the cellulose, the pentosans, are removed by the acid treatment converted into sugar and can be used usefully in this way.
Eingroßes Problem bei der Hefeerzeugung bilden die Abwässer. Diese Abwässer enthalten noch ungenutzten Zucker und unausgenutzte anorganische Nährsalze neben Hefezellen und bilden -einen richtigen Nährboden für allerlei Pflänzchen und Pilzwucherungen. Diese Abwässer-müssen, ehe sie in die Flüsse gelassen -werden, in großen Vorflutern sehr stark verdünnt werden; aber auch dann wirken sie sich noch schädigend im Flußwasser aus, und zwar wegen ihres großen Sauerstoffverbrauchs. Es wurde nun gefunden, daß man die Hefeabwässer aus den Hefeseparatoren nutzbringend verwerten kann, wenn man sie zum Verdünnen .der für die Vorhydrolyse erforderlichen Säurelösungen benutzt. Auf diese Weise wird das Abwasser nicht mit Hefesuspensionen verunreinigt und gleichzeitig durch die Rückführung der Hefeabwässer unausgenutzter Zucker und im Wasser noch befindliche- anorganische .Nährsalze nochmals verwertet.Wastewater is a major problem in yeast production. These Wastewater still contains unused sugar and unused inorganic nutrient salts in addition to yeast cells and form a real breeding ground for all kinds of plants and Mushroom growths. This sewage, before it is released into the rivers, be very heavily diluted in large receiving waters; but even then they have an effect still harmful in the river water, because of their high oxygen consumption. It has now been found that the yeast effluents from the yeast separators can be beneficial can be used if they can be used to dilute the ones required for pre-hydrolysis Acid solutions used. This way, the wastewater will not contain yeast suspensions contaminated and at the same time unused due to the recirculation of the yeast waste water Sugar and inorganic nutrient salts still in the water are reused.
Nach einem bekannten Verfahrender Hydrolyse von cellulosehaltigen Stoffen wird die beider Vergärung von Zucker auf Alkohol anfallende Schlempe unter Zusatz frischen polymeren Zuckers einer erneuten sauren Kochung zwecks Alkoholgewinnung unterworfen; auch wenn die bei der Holzhydrolyse anfallende saure Flüssigkeit nicht auf Alkohol oder auf Milchsäure, sondern auf Hefe verarbeitet werden soll, können die hier anfallenden Schlempen wieder zum Teil in die Hauptgärung rückgeleitet werden.According to a known method of hydrolysis of cellulose-containing The fermentation from sugar to alcohol is submerged in the stillage resulting from the fermentation of alcohol Addition of fresh polymeric sugar from a renewed acidic boil for the purpose of alcohol production subjected; even if the acidic liquid resulting from wood hydrolysis is not on alcohol or on lactic acid, but on yeast, can be processed some of the stillage produced here is returned to the main fermentation process.
Von diesem bekannten Verfahren unterscheidet sich das erfindungsgemäße jedoch dadurch, daß die nach der Hefegärung verbleibenden Wässer der sauren Vorhydrolyse restlos wieder zugeführt werden, um einmal die lästigen Hefeabwässer, die zu einer starken Verunreinigung der Flüsse führen, zu beseitigen; -ünc'zurn` anderen, um den Säuregrad in der Vorhydrolyse auf das richtige Verhältnis abzustimmen.The method according to the invention differs from this known method however, in that the water remaining after the yeast fermentation of the acidic pre-hydrolysis completely be fed back to once the pesky yeast effluents that lead to strong Pollution of rivers lead to eliminate; -to 'to' another, about the degree of acidity to match the correct ratio in the pre-hydrolysis.
Die Verwendung der Hefeabwässer zum Verdünnen der Vorhydrolysesäure ist möglich, weil im Kochprozeß beider Vorhydrolyse die Hefezellen des zugeführten Hefeabwasser zerstört und das Hefeeiweiß bis zu denAminosäuren abgebaut wird. Man .erreicht dadurcli den Vorteil, daß man in den unter Verwendung der Hefeabwässer hergestellten Hydrolysaten außer den anorganischen Stickstoffsalzen noch Stickstoff in organischer Bindung besitzt, der wesentlich fördernd auf die Hefeausbeute einwirkt und den in geringer Menge vorhandenen Stickstoff in organischer Bindung aus dem cellulosehaltigen Rohstoff, beispielsweise aus dem zür Hydrolyse eingesetzten Stroh, noch vermehrt. Es ist also durch das Verfahren möglich, die Hefeausbeuten zu steigern.The use of the yeast effluent to dilute the prehydrolysis acid is possible because the yeast cells of the supplied during the cooking process during both pre-hydrolysis Yeast wastewater is destroyed and the yeast protein is broken down down to the amino acids. Man Dadurcli achieves the advantage that one can use the yeast wastewater in the produced hydrolysates in addition to the inorganic nitrogen salts and nitrogen has an organic bond, which has a significantly promoting effect on the yeast yield and the nitrogen present in small amounts in organic bond from the cellulose-containing raw material, for example from the straw used for hydrolysis, even more. The process therefore makes it possible to increase the yeast yields.
Die zurückgeführten Separatorabwässer entsprechen ungefähr dem Bedarf an Verdünnungswasser, so daß ein Anfall an Abwasser nicht oder nur in geringem Maße eintritt. Die Wiederverwendung der Hefeabwässer ist nur möglich, weil diese mit der Hydrolyse unterworfen werden. Die an und für sich nicht schädlichen anorganischen Salze im Hefeabwasser, wie Arnmonumsulfat, Phosphate. Kalium- und Magnesiumsulfat,werden ebenfalls ausgenützt. Dies ist insbesondere im Hinblick auf d.ie Phosphate von Wichtigkeit, die an und für sich teuer sind und nicht in größeren Mengen zur Verfügung stellen.The recycled separator wastewater corresponds roughly to the demand of dilution water, so that an accumulation of wastewater is not or only to a small extent entry. The reuse of the yeast wastewater is only possible because this is with are subjected to hydrolysis. The inorganic ones, which are not harmful in and of themselves Salts in yeast wastewater, such as ammonium sulfate, phosphates. Potassium and magnesium sulfate also exploited. This is particularly important with regard to the phosphates, which are expensive in and of themselves and are not available in large quantities.
Sollte durch die Rückführung der Hefeabwässer im Vorhydrolysat eineAnreicherung von .die Gärung hemmenden Stoffen eintreten, so können diese durch eine geeignete Behandlung :aus der Zuckerlösung entfernt werden. An und für sich ist aber .eine Entfernung gärungshemmender Stoffe, die ja im allgemeinen Eiweißkörper sind, durch eine besondere Vorbehandlung nicht erforderlich; denn ebenso wie die Hefezellen in den Abwässern bei Verwendung als Verdünnungsmittel als Eiweißstoffe zum größten Teil hydrolytisch aufgespalten werden, werden natürlich auch die l;äruiigshemmendeii Stoffe, da sie vbeilfalls in der Hauptsache Eiweißprodukte sind, weitgehendst liydrolysiert und damit ungiftig gemacht. Sollte eine Reinigung erforderlich sein, so kann sie beispielsweise in der Weise durchgeführt werden, daß die Hydrolysate mittels Kalkmilch stark alkalisch gemacht werden und der nach längerem Stehen sich abscheidende Niederschlag abfiltriert wird. Das Filtrat wird dann mit Schwefelsäure schwach angesäuert (bis zu px 4-5). Durch den hierbei fein verteilt ausfallenden Gips werden wiederum organische Kolloidstoffe mitgerissen, so daß eine zweite Reinigung stattfindet. Von dem abgeschiedenen Gips wird dann nochmals abfiltriert.Should an enrichment through the return of the yeast waste water in the pre-hydrolyzate of. the fermentation-inhibiting substances occur, these can be prevented by a suitable Treatment: to be removed from the sugar solution. In and of itself, however, is a Removal of fermentation-inhibiting substances, which are generally protein bodies no special pre-treatment is required; because just like the yeast cells in the wastewater when used as a diluent as protein substances to the greatest extent Partly hydrolytically split up are naturally also the oil inhibitors Substances, since they are mostly protein products, are largely lyydrolysed and thus made non-toxic. Should cleaning be necessary, it can for example be carried out in such a way that the hydrolysates by means of milk of lime can be made strongly alkaline and the precipitate that separates out after prolonged standing is filtered off. The filtrate is then weakly acidified with sulfuric acid (up to to px 4-5). The plaster of paris, which is then finely distributed, turns organic Colloids are entrained so that a second cleaning takes place. From the departed Gypsum is then filtered off again.
Das Verfahren :der Erfindung kann auf alle Vorhydrolysate von cellulosehaltigen Rohstoffen Verwendung finden. Als cellulosehaltige Rohstoffe kommen beispielsweise in Betracht: Buchenholz, Fichtenholz, Stroh, Kartoffelkraut oder Gräser. Beispielsweise kann die Vorhydrolyse durch Druckkochung von Stroh mit o,5o/oiger Schwefelsäure bei 13o° und bei einem Druck von 1,5 atü durchgeführt werden. Nach4Stunden Einwirkungsdauer wird die Vorhydrolyse abgebrochen und die saure Flüssigkeit abgelassen und auf Hefe verarbeitet, während der Rückstand auf Zellstoff verarbeitet wird. DieSäurekonzentration der Hydrolysiersäure wird in der Regel so eingestellt, daß sie eine Konzentration von z bis z °/o wesentlich überschreitet.The method: the invention can be applied to all prehydrolysates of cellulose-containing Find use of raw materials. For example, cellulose-containing raw materials are used Considered: beech wood, spruce wood, straw, potato tops or grasses. For example Prehydrolysis can be achieved by pressure boiling straw with 0.5% sulfuric acid at 130 ° and at a pressure of 1.5 atü. After 4 hours of exposure the pre-hydrolysis is stopped and the acidic liquid is drained off and on yeast processed while the residue on pulp is processed. The acid concentration the hydrolyzing acid is usually adjusted so that it has a concentration from z to z% significantly exceeds.
Außer Schwefelsäure können zur Vorhydrolyse andere anorganische Säuren, wie beispielsweise Salzsäure oder Phosphorsäure, oder auch organische Säuren, wie Essigsäure, benutzt werden.In addition to sulfuric acid, other inorganic acids, such as hydrochloric acid or phosphoric acid, or organic acids, such as Acetic acid.
Claims (1)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DEP83422D DE746534C (en) | 1941-12-03 | 1941-12-03 | Utilization of yeast waste water |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DEP83422D DE746534C (en) | 1941-12-03 | 1941-12-03 | Utilization of yeast waste water |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE746534C true DE746534C (en) | 1944-12-22 |
Family
ID=7394146
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DEP83422D Expired DE746534C (en) | 1941-12-03 | 1941-12-03 | Utilization of yeast waste water |
Country Status (1)
| Country | Link |
|---|---|
| DE (1) | DE746534C (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE944182C (en) * | 1945-04-19 | 1956-06-07 | Phrix Werke Ag | Process for the production of harvestable prehydrolysates |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE599476C (en) * | 1929-07-28 | 1934-07-03 | Holzhydrolyse Akt Ges | Process for preparing vinasse from cellulose sugar for re-fermentation |
-
1941
- 1941-12-03 DE DEP83422D patent/DE746534C/en not_active Expired
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE599476C (en) * | 1929-07-28 | 1934-07-03 | Holzhydrolyse Akt Ges | Process for preparing vinasse from cellulose sugar for re-fermentation |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE944182C (en) * | 1945-04-19 | 1956-06-07 | Phrix Werke Ag | Process for the production of harvestable prehydrolysates |
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