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DE69902796D1 - Verfahren zur reinigung von biomolekül- oder proteinkomplexen - Google Patents

Verfahren zur reinigung von biomolekül- oder proteinkomplexen

Info

Publication number
DE69902796D1
DE69902796D1 DE69902796T DE69902796T DE69902796D1 DE 69902796 D1 DE69902796 D1 DE 69902796D1 DE 69902796 T DE69902796 T DE 69902796T DE 69902796 T DE69902796 T DE 69902796T DE 69902796 D1 DE69902796 D1 DE 69902796D1
Authority
DE
Germany
Prior art keywords
biomolecule
purifying
protein complexes
vectors
detecting
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69902796T
Other languages
English (en)
Other versions
DE69902796T2 (de
Inventor
Bertrand Seraphin
Guillaume Rigaut
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Europaisches Laboratorium fuer Molekularbiologie EMBL
Original Assignee
Europaisches Laboratorium fuer Molekularbiologie EMBL
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Europaisches Laboratorium fuer Molekularbiologie EMBL filed Critical Europaisches Laboratorium fuer Molekularbiologie EMBL
Publication of DE69902796D1 publication Critical patent/DE69902796D1/de
Application granted granted Critical
Publication of DE69902796T2 publication Critical patent/DE69902796T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/40—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/705—Fusion polypeptide containing domain for protein-protein interaction containing a protein-A fusion

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
DE69902796T 1998-08-17 1999-08-17 Verfahren zur reinigung von biomolekül- oder proteinkomplexen Expired - Lifetime DE69902796T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP98115448 1998-08-17
PCT/EP1999/006022 WO2000009716A1 (en) 1998-08-17 1999-08-17 Method for purifying proteins and/or biomolecule or protein complexes

Publications (2)

Publication Number Publication Date
DE69902796D1 true DE69902796D1 (de) 2002-10-10
DE69902796T2 DE69902796T2 (de) 2003-08-07

Family

ID=8232468

Family Applications (2)

Application Number Title Priority Date Filing Date
DE69902796T Expired - Lifetime DE69902796T2 (de) 1998-08-17 1999-08-17 Verfahren zur reinigung von biomolekül- oder proteinkomplexen
DE1105508T Pending DE1105508T1 (de) 1998-08-17 1999-08-17 Verfahren zur reinigung von proteinen und biomolekül- oder proteinkomplexen

Family Applications After (1)

Application Number Title Priority Date Filing Date
DE1105508T Pending DE1105508T1 (de) 1998-08-17 1999-08-17 Verfahren zur reinigung von proteinen und biomolekül- oder proteinkomplexen

Country Status (11)

Country Link
US (2) US20020061513A1 (de)
EP (2) EP1105508B1 (de)
JP (1) JP3676978B2 (de)
AT (1) ATE223491T1 (de)
AU (1) AU762961B2 (de)
CA (1) CA2340974C (de)
DE (2) DE69902796T2 (de)
DK (1) DK1105508T3 (de)
ES (1) ES2183601T3 (de)
PT (1) PT1105508E (de)
WO (1) WO2000009716A1 (de)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7192596B2 (en) * 1996-08-23 2007-03-20 The Health Protection Agency Ipsen Limited Recombinant toxin fragments
JP3676978B2 (ja) * 1998-08-17 2005-07-27 ユーロペーイシェ ラボラトリウム フュール モレキュラーバイオロジー（イーエムビーエル） タンパク質および／または生体分子もしくはタンパク質複合体の精製方法
FR2830020B1 (fr) * 2001-09-25 2003-12-19 Univ Victor Segalen Bordeaux 2 Acide nucleique pour l'obtention d'un polynucleotide codant une proteine de detection et d'isolement d'un ligand "proie" et applications de cet acide nucleique
US7825227B2 (en) * 2002-05-09 2010-11-02 Prolexys Pharmaceuticals, Inc. Method for purification of a protein complex and identification of its components
CA2463301A1 (en) * 2004-04-22 2005-10-22 Atanas Iliev Lalev Method for detecting protein interactions - affinity purification with switching the ligand
AU2003298171A1 (en) * 2003-09-05 2005-03-29 Cellzome Ag Protein complexes associated with app-processing
US7238478B2 (en) 2003-11-13 2007-07-03 Stratagene California Compositions and methods for protein isolation
WO2006070416A1 (en) * 2004-12-30 2006-07-06 Fondazione Santa Lucia I.R.C.C.S. An immunoprecipitation-based method to purify and characterise biological macromolecular complexes
WO2006081430A2 (en) * 2005-01-27 2006-08-03 Five Prime Therapeutics, Inc. Leader sequences for directing secretion of polypeptides and methods for production thereof
EP2423218B1 (de) 2009-04-22 2014-06-11 Osaka University Markierungspeptid mit proteaseerkennungssequenz und verwendung
FR2944799B1 (fr) * 2009-04-28 2011-05-13 Ecole De Biolog Ind Ebi Procede de production d'une proteine d'interet grace a une nouvelle proteine de fusion.
WO2016145416A2 (en) * 2015-03-11 2016-09-15 The Broad Institute, Inc. Proteomic analysis with nucleic acid identifiers
EP3769083A1 (de) 2018-03-21 2021-01-27 Waters Technologies Corporation Verfahren zur vorbereitung einer hochaffinen nicht-antikörper-probe, sorbenzien, vorrichtungen und verfahren

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5821088A (en) * 1990-05-11 1998-10-13 Siga Pharmaceuticals, Inc. Use of gram-positive bacteria to express recombinant proteins
JP3676978B2 (ja) * 1998-08-17 2005-07-27 ユーロペーイシェ ラボラトリウム フュール モレキュラーバイオロジー（イーエムビーエル） タンパク質および／または生体分子もしくはタンパク質複合体の精製方法

Also Published As

Publication number Publication date
ES2183601T3 (es) 2003-03-16
EP1231276A1 (de) 2002-08-14
EP1105508A1 (de) 2001-06-13
JP2002522085A (ja) 2002-07-23
AU762961B2 (en) 2003-07-10
CA2340974C (en) 2010-01-12
DE1105508T1 (de) 2002-02-21
PT1105508E (pt) 2003-01-31
DE69902796T2 (de) 2003-08-07
DK1105508T3 (da) 2003-01-06
JP3676978B2 (ja) 2005-07-27
AU5736299A (en) 2000-03-06
ATE223491T1 (de) 2002-09-15
WO2000009716A1 (en) 2000-02-24
CA2340974A1 (en) 2000-02-24
EP1105508B1 (de) 2002-09-04
US20090042216A1 (en) 2009-02-12
US20020061513A1 (en) 2002-05-23

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition