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DE69739263D1 - Produktion von proteinen, dafür kodierende plasmide und organismen, die solche plasmide enthalten - Google Patents

Produktion von proteinen, dafür kodierende plasmide und organismen, die solche plasmide enthalten

Info

Publication number
DE69739263D1
DE69739263D1 DE69739263T DE69739263T DE69739263D1 DE 69739263 D1 DE69739263 D1 DE 69739263D1 DE 69739263 T DE69739263 T DE 69739263T DE 69739263 T DE69739263 T DE 69739263T DE 69739263 D1 DE69739263 D1 DE 69739263D1
Authority
DE
Germany
Prior art keywords
plasmids
pct
coke
proteins
production
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69739263T
Other languages
English (en)
Inventor
Matthew Guy Duchars
Andrew William Topping
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Fujifilm Diosynth Biotechnologies UK Ltd
Original Assignee
Avecia Biologics Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Avecia Biologics Ltd filed Critical Avecia Biologics Ltd
Application granted granted Critical
Publication of DE69739263D1 publication Critical patent/DE69739263D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67General methods for enhancing the expression
    • C12N15/68Stabilisation of the vector
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70Vectors or expression systems specially adapted for E. coli
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477Hemicellulases not provided in a preceding group
    • C12N9/248Xylanases
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y302/00Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01008Endo-1,4-beta-xylanase (3.2.1.8)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y302/00Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01032Xylan endo-1,3-beta-xylosidase (3.2.1.32), i.e. endo-1-3-beta-xylanase

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Peptides Or Proteins (AREA)
DE69739263T 1996-02-22 1997-02-20 Produktion von proteinen, dafür kodierende plasmide und organismen, die solche plasmide enthalten Expired - Lifetime DE69739263D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB9603803.9A GB9603803D0 (en) 1996-02-22 1996-02-22 Production of proteins, plasmids coding therefor and organisms containing such plasmids
PCT/GB1997/000465 WO1997031118A1 (en) 1996-02-22 1997-02-20 Production of proteins, plasmids coding therefor and organisms containing such plasmids

Publications (1)

Publication Number Publication Date
DE69739263D1 true DE69739263D1 (de) 2009-04-02

Family

ID=10789244

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69739263T Expired - Lifetime DE69739263D1 (de) 1996-02-22 1997-02-20 Produktion von proteinen, dafür kodierende plasmide und organismen, die solche plasmide enthalten

Country Status (11)

Country Link
US (1) US6136567A (de)
EP (1) EP0889965B1 (de)
JP (1) JP2000504590A (de)
AT (1) ATE423211T1 (de)
AU (1) AU1884997A (de)
CA (1) CA2247162A1 (de)
DE (1) DE69739263D1 (de)
DK (1) DK0889965T3 (de)
GB (1) GB9603803D0 (de)
WO (1) WO1997031118A1 (de)
ZA (1) ZA971477B (de)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE60239386D1 (de) * 2001-01-25 2011-04-21 Evolva Ltd Zellbibliothek
US8008459B2 (en) * 2001-01-25 2011-08-30 Evolva Sa Concatemers of differentially expressed multiple genes
EP1308517A1 (de) * 2001-10-31 2003-05-07 Aventis Pharmacueticals Products Inc. Vektoren für die Expression von mehrere Transgenen
JP4472345B2 (ja) * 2002-01-25 2010-06-02 エヴォルヴァ・リミテッド 複数パラメータースクリーニングおよび複数機能性小分子産生細胞への進化方法
US20060252156A1 (en) * 2002-08-01 2006-11-09 Evolva Ltd. C/O Dr. Iur. Martin Eisenring Methods of mixing large numbers of heterologous genes

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DD233852B1 (de) * 1984-10-03 1987-11-18 Adw Ddr Verfahren zur herstellung von alpha-amylase
US5017488A (en) * 1986-04-01 1991-05-21 University Of Medicine And Dentistry Of New Jersey Highly efficient dual T7/T3 promoter vector PJKF16 and dual SP6/T3 promoter vector PJFK15
WO1989000605A1 (en) * 1987-07-16 1989-01-26 Codon Transfected cells containing plasmids having genes oriented in opposing directions and methods of obtaining the same
US5460954A (en) * 1992-04-01 1995-10-24 Cheil Foods & Chemicals, Inc. Production of human proinsulin using a novel vector system

Also Published As

Publication number Publication date
EP0889965A1 (de) 1999-01-13
JP2000504590A (ja) 2000-04-18
US6136567A (en) 2000-10-24
GB9603803D0 (en) 1996-04-24
ZA971477B (en) 1997-08-22
EP0889965B1 (de) 2009-02-18
ATE423211T1 (de) 2009-03-15
DK0889965T3 (da) 2009-05-18
WO1997031118A1 (en) 1997-08-28
CA2247162A1 (en) 1997-08-28
AU1884997A (en) 1997-09-10

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