DE69738135D1 - Modifizierte methylotrophe p. pastoris hefe, welche menschliches wachstumshormon sekretiert - Google Patents
Modifizierte methylotrophe p. pastoris hefe, welche menschliches wachstumshormon sekretiertInfo
- Publication number
- DE69738135D1 DE69738135D1 DE69738135T DE69738135T DE69738135D1 DE 69738135 D1 DE69738135 D1 DE 69738135D1 DE 69738135 T DE69738135 T DE 69738135T DE 69738135 T DE69738135 T DE 69738135T DE 69738135 D1 DE69738135 D1 DE 69738135D1
- Authority
- DE
- Germany
- Prior art keywords
- hgh
- growth hormone
- human growth
- methylotrophic yeast
- copy
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 102000002265 Human Growth Hormone Human genes 0.000 title abstract 5
- 108010000521 Human Growth Hormone Proteins 0.000 title abstract 5
- 239000000854 Human Growth Hormone Substances 0.000 title abstract 5
- 240000004808 Saccharomyces cerevisiae Species 0.000 title abstract 5
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 abstract 3
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 3
- 241000235058 Komagataella pastoris Species 0.000 abstract 1
- NPBGTPKLVJEOBE-IUCAKERBSA-N Lys-Arg Chemical compound NCCCC[C@H](N)C(=O)N[C@H](C(O)=O)CCCNC(N)=N NPBGTPKLVJEOBE-IUCAKERBSA-N 0.000 abstract 1
- IXKSXJFAGXLQOQ-XISFHERQSA-N WHWLQLKPGQPMY Chemical compound C([C@@H](C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC(C)C)C(=O)N1CCC[C@H]1C(=O)NCC(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC(O)=O)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(O)=O)NC(=O)[C@@H](N)CC=1C2=CC=CC=C2NC=1)C1=CNC=N1 IXKSXJFAGXLQOQ-XISFHERQSA-N 0.000 abstract 1
- 239000002299 complementary DNA Substances 0.000 abstract 1
- 239000001963 growth medium Substances 0.000 abstract 1
- 230000001939 inductive effect Effects 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 230000002797 proteolythic effect Effects 0.000 abstract 1
- 230000028327 secretion Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/61—Growth hormone [GH], i.e. somatotropin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
- C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- General Engineering & Computer Science (AREA)
- Mycology (AREA)
- Biotechnology (AREA)
- Endocrinology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Physics & Mathematics (AREA)
- Toxicology (AREA)
- Plant Pathology (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Gastroenterology & Hepatology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MX9605082A MX9605082A (es) | 1996-10-24 | 1996-10-24 | Levaduras metilotroficas modificadas geneticamente para la produccion y secrecion de hormona de crecimiento humano. |
| PCT/MX1997/000033 WO1998020035A1 (es) | 1996-10-24 | 1997-10-24 | Levadura metilotrofica modificada de p. pastoris de secrecion de hormona de crecimiento humano |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE69738135D1 true DE69738135D1 (de) | 2007-10-25 |
Family
ID=19744934
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE69738135T Expired - Fee Related DE69738135D1 (de) | 1996-10-24 | 1997-10-24 | Modifizierte methylotrophe p. pastoris hefe, welche menschliches wachstumshormon sekretiert |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US6342375B1 (de) |
| EP (1) | EP0952158B1 (de) |
| AT (1) | ATE373097T1 (de) |
| DE (1) | DE69738135D1 (de) |
| MX (1) | MX9605082A (de) |
| WO (1) | WO1998020035A1 (de) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2000078978A1 (en) * | 1999-06-24 | 2000-12-28 | Zymogenetics, Inc. | Pichia methanolica glyceraldehyde 3-phosphate dehydrogenase 1 promoter and terminator |
| DE10252245A1 (de) * | 2002-11-07 | 2004-05-27 | Prof. Dr. Danilo Porro Università degli Studi di Milano-Bicocca Dipartimento die Biotechnologie e Bioscienze | Verfahren zur Expression und Sekretion von Proteinen mittels der nicht-konventionellen Hefe Zygosaccharomyces bailii |
| WO2004108890A2 (en) * | 2003-05-30 | 2004-12-16 | New Century Pharmaceuticals, Inc. | Dilute methanol -or ethanol- induced production of polypeptides in microorganisms |
| CN100543141C (zh) * | 2003-09-16 | 2009-09-23 | 周琪 | 一种生产基因工程重组192肽人生长激素的方法 |
| CN100434522C (zh) * | 2003-11-21 | 2008-11-19 | 上海新生源医药研究有限公司 | 重组人生长激素的生产方法 |
| KR100594607B1 (ko) * | 2004-11-03 | 2006-06-30 | 재단법인서울대학교산학협력재단 | 신규한 경구투여용 재조합 인간 성장호르몬 분비미생물제제 및 그 제조방법 |
| EP2182060B1 (de) * | 2005-08-03 | 2012-10-24 | Asahi Glass Company, Limited | Transformierte Hefezellen und Verfahren zur Herstellung von Fremdprotein |
| KR101521674B1 (ko) * | 2008-04-03 | 2015-05-19 | 바이오스티드 진 익스프레션 테크. 컴파니 리미티드 | 이중-가닥 폴리에틸렌 글리콜-수식된 성장 호르몬, 이의 제조 방법 및 용도 |
| WO2011051964A2 (en) | 2009-10-07 | 2011-05-05 | Reliance Life Sciences Pvt. Ltd. | Process for recombinant human growth hormone |
| CN115698251A (zh) * | 2020-05-21 | 2023-02-03 | 阿贝拉实验室公司 | 需氧发酵系统及使用其的方法 |
Family Cites Families (32)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4342832A (en) * | 1979-07-05 | 1982-08-03 | Genentech, Inc. | Method of constructing a replicable cloning vehicle having quasi-synthetic genes |
| NZ199722A (en) | 1981-02-25 | 1985-12-13 | Genentech Inc | Dna transfer vector for expression of exogenous polypeptide in yeast;transformed yeast strain |
| US4755465A (en) | 1983-04-25 | 1988-07-05 | Genentech, Inc. | Secretion of correctly processed human growth hormone in E. coli and Pseudomonas |
| US4885242A (en) | 1984-10-30 | 1989-12-05 | Phillips Petroleum Company | Genes from pichia histidine pathway and uses thereof |
| US4855231A (en) | 1984-10-30 | 1989-08-08 | Phillips Petroleum Company | Regulatory region for heterologous gene expression in yeast |
| US4837148A (en) | 1984-10-30 | 1989-06-06 | Phillips Petroleum Company | Autonomous replication sequences for yeast strains of the genus pichia |
| US4879231A (en) | 1984-10-30 | 1989-11-07 | Phillips Petroleum Company | Transformation of yeasts of the genus pichia |
| US4808537A (en) | 1984-10-30 | 1989-02-28 | Phillips Petroleum Company | Methanol inducible genes obtained from pichia and methods of use |
| US5166329A (en) | 1985-10-25 | 1992-11-24 | Phillips Petroleum Company | DNA encoding the alcohol oxidase 2 gene of yeast of the genus Pichia |
| US4818700A (en) | 1985-10-25 | 1989-04-04 | Phillips Petroleum Company | Pichia pastoris argininosuccinate lyase gene and uses thereof |
| US4882279A (en) | 1985-10-25 | 1989-11-21 | Phillips Petroleum Company | Site selective genomic modification of yeast of the genus pichia |
| US5032516A (en) | 1985-10-25 | 1991-07-16 | Phillips Petroleum Company | Pichia pastoris alcohol oxidase II regulatory region |
| US4812405A (en) | 1986-02-18 | 1989-03-14 | Phillips Petroleum Company | Double auxotrophic mutants of Pichia pastoris and methods for preparation |
| US4857467A (en) | 1986-07-23 | 1989-08-15 | Phillips Petroleum Company | Carbon and energy source markers for transformation of strains of the genes Pichia |
| US5002876A (en) | 1986-09-22 | 1991-03-26 | Phillips Petroleum Company | Yeast production of human tumor necrosis factor |
| US4683293A (en) | 1986-10-20 | 1987-07-28 | Phillips Petroleum Company | Purification of pichia produced lipophilic proteins |
| IT1228925B (it) | 1987-08-07 | 1991-07-10 | Eniricerche Spa | Procedimento per la preparazione dell'ormone della crescita umano |
| US4929555A (en) | 1987-10-19 | 1990-05-29 | Phillips Petroleum Company | Pichia transformation |
| SE8901264L (sv) * | 1988-04-11 | 1989-10-12 | Phillips Petroleum Co | Uttryck av laxtillvaexthormon i metylotropisk jaest av slaektet pichia |
| US5004688A (en) | 1988-04-15 | 1991-04-02 | Phillips Petroleum Company | Purification of hepatitis proteins |
| IL89992A0 (en) | 1988-04-25 | 1989-12-15 | Phillips Petroleum Co | Expression of human serum albumin in methylotrophic yeasts |
| NZ228948A (en) | 1988-05-13 | 1991-06-25 | Phillips Petroleum Co | Hbv particles containing s and pre-s 2 proteins and recombinant processes for their production in yeast cells |
| US5102789A (en) | 1989-03-15 | 1992-04-07 | The Salk Institute Biotechnology/Industrial Associates, Inc. | Production of epideramal growth factor in pichia pastoris yeast cells |
| US5122465A (en) | 1989-06-12 | 1992-06-16 | Phillips Petroleum Company | Strains of pichia pastoris created by interlocus recombination |
| EP0548267A4 (en) * | 1990-09-04 | 1994-11-23 | Salk Inst Biotech Ind | Production of insulin-like growth factor-1 in methylotrophic yeast cells |
| US5268273A (en) | 1990-12-14 | 1993-12-07 | Phillips Petroleum Company | Pichia pastoris acid phosphatase gene, gene regions, signal sequence and expression vectors comprising same |
| CA2058820C (en) | 1991-04-25 | 2003-07-15 | Kotikanyad Sreekrishna | Expression cassettes and vectors for the secretion of human serum albumin in pichia pastoris cells |
| US5330901A (en) | 1991-04-26 | 1994-07-19 | Research Corporation Technologies, Inc. | Expression of human serum albumin in Pichia pastoris |
| CA2101610A1 (en) | 1992-08-07 | 1994-02-08 | William D. Prevatt | Production of bacillus entomotoxins in methylotrophic yeast |
| EP0852625A2 (de) | 1995-09-25 | 1998-07-15 | Chiron Corporation | Sekretorische 'leader'-sequenz aus pichia zur protein-expression |
| GB9526733D0 (en) * | 1995-12-30 | 1996-02-28 | Delta Biotechnology Ltd | Fusion proteins |
| IL140553A0 (en) | 1998-07-03 | 2002-02-10 | Res Corp Technologies Inc | Formaldehyde dehydrogenase genes from methylotrophic yeasts |
-
1996
- 1996-10-24 MX MX9605082A patent/MX9605082A/es unknown
-
1997
- 1997-10-24 DE DE69738135T patent/DE69738135D1/de not_active Expired - Fee Related
- 1997-10-24 AT AT97909744T patent/ATE373097T1/de not_active IP Right Cessation
- 1997-10-24 EP EP97909744A patent/EP0952158B1/de not_active Expired - Lifetime
- 1997-10-24 US US09/284,878 patent/US6342375B1/en not_active Expired - Fee Related
- 1997-10-24 WO PCT/MX1997/000033 patent/WO1998020035A1/es active IP Right Grant
Also Published As
| Publication number | Publication date |
|---|---|
| EP0952158A4 (de) | 2001-10-24 |
| ATE373097T1 (de) | 2007-09-15 |
| MX9605082A (es) | 1998-04-30 |
| WO1998020035A1 (es) | 1998-05-14 |
| EP0952158B1 (de) | 2007-09-12 |
| EP0952158A1 (de) | 1999-10-27 |
| US6342375B1 (en) | 2002-01-29 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8339 | Ceased/non-payment of the annual fee |