DE69627532T2 - Helfervirus-freie herstellung von aav - Google Patents

Helfervirus-freie herstellung von aav Download PDF

Info

Publication number: DE69627532T2
Authority: DE; Germany
Prior art keywords: helper virus; aav; free manufacture; virus; manufacture
Prior art date: 1995-06-07
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Expired - Lifetime

Application number

DE69627532T

Other languages

English (en)

Other versions

DE69627532D1 (de

Inventor

K Ferrari

Xiao Xiao

J Samulski

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

University of North Carolina at Chapel Hill

Original Assignee

University of North Carolina at Chapel Hill

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1995-06-07

Filing date

1996-06-06

Publication date

2004-01-29

1996-06-06 Application filed by University of North Carolina at Chapel Hill filed Critical University of North Carolina at Chapel Hill

2003-05-22 Application granted granted Critical

2003-05-22 Publication of DE69627532D1 publication Critical patent/DE69627532D1/de

2004-01-29 Publication of DE69627532T2 publication Critical patent/DE69627532T2/de

2016-06-07 Anticipated expiration legal-status Critical

Status Expired - Lifetime legal-status Critical Current

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/86—Viral vectors
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
- C12N2750/00011—Details
- C12N2750/14011—Parvoviridae
- C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
- C12N2750/14141—Use of virus, viral particle or viral elements as a vector
- C12N2750/14143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector

Landscapes

Life Sciences & Earth Sciences (AREA)
Health & Medical Sciences (AREA)
Genetics & Genomics (AREA)
Engineering & Computer Science (AREA)
Zoology (AREA)
Bioinformatics & Cheminformatics (AREA)
Biomedical Technology (AREA)
Organic Chemistry (AREA)
Biotechnology (AREA)
General Engineering & Computer Science (AREA)
Chemical & Material Sciences (AREA)
Wood Science & Technology (AREA)
Microbiology (AREA)
Physics & Mathematics (AREA)
Plant Pathology (AREA)
Virology (AREA)
Molecular Biology (AREA)
Biochemistry (AREA)
General Health & Medical Sciences (AREA)
Biophysics (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)

DE69627532T 1995-06-07 1996-06-06 Helfervirus-freie herstellung von aav Expired - Lifetime DE69627532T2 (de)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
US48375795A	1995-06-07	1995-06-07
PCT/US1996/009405 WO1996040240A1 (en)	1995-06-07	1996-06-06	Helper virus-free aav production

Publications (2)

Publication Number	Publication Date
DE69627532D1 DE69627532D1 (de)	2003-05-22
DE69627532T2 true DE69627532T2 (de)	2004-01-29

Family

ID=23921407

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
DE69627532T Expired - Lifetime DE69627532T2 (de)	1995-06-07	1996-06-06	Helfervirus-freie herstellung von aav

Country Status (7)

Country	Link
EP (1)	EP0871483B1 (de)
JP (1)	JP4108746B2 (de)
AT (1)	ATE237352T1 (de)
AU (1)	AU707862B2 (de)
CA (1)	CA2221293C (de)
DE (1)	DE69627532T2 (de)
WO (1)	WO1996040240A1 (de)

Families Citing this family (24)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
EP0850313B8 (de) *	1995-09-08	2009-07-29	Genzyme Corporation	Verbesserte aav vektoren für die gentherapie
US6004797A (en)	1995-11-09	1999-12-21	Avigen, Inc.	Adenovirus helper-free recombinant AAV Virion production
US6221646B1 (en)	1997-07-31	2001-04-24	Chiron Corporation	Materials and methods for simplified AAV production
CA2303768C (en) *	1997-09-19	2009-11-24	The Trustees Of The University Of Pennsylvania	Methods and vector constructs useful for production of recombinant aav
US6953690B1 (en)	1998-03-20	2005-10-11	The Trustees Of The University Of Pennsylvania	Compositions and methods for helper-free production of recombinant adeno-associated viruses
PT1064393E (pt) *	1998-03-20	2005-04-29	Univ Pennsylvania	Composicoes e metodos para a producao de virus adeno-associados recombinantes sem auxiliar
MXPA01004169A (es) *	1998-10-27	2002-06-04	Crucell Holland Bv	Produccion mejorada de vector de virus adenoasociados.
JP4693244B2 (ja)	1999-03-18	2011-06-01	ザ・トラステイーズ・オブ・ザ・ユニバーシテイ・オブ・ペンシルベニア	組換えアデノ随伴ウイルスのヘルパー無しの生産のための組成物および方法
US6258595B1 (en) *	1999-03-18	2001-07-10	The Trustees Of The University Of Pennsylvania	Compositions and methods for helper-free production of recombinant adeno-associated viruses
AU2001257611A1 (en)	2000-04-28	2001-11-12	Avigen, Inc.	Polynucleotides for use in recombinant adeno-associated virus virion production
AU2013326503B2 (en) *	2012-10-03	2018-04-19	Glaxosmithkline Biologicals Sa	Immunogenic composition
AU2016347887B2 (en)	2015-11-05	2019-11-14	Bamboo Therapeutics, Inc.	Modified friedreich ataxia genes and vectors for gene therapy
CA2971303A1 (en)	2016-06-21	2017-12-21	Bamboo Therapeutics, Inc.	Optimized mini-dystrophin genes and expression cassettes and their use
WO2020261178A1 (en)	2019-06-27	2020-12-30	Pfizer Inc.	Methods of treating duchenne muscular dystrophy using aav mini-dystrophin gene therapy
IL302608A (en)	2020-11-03	2023-07-01	Pfizer	Methods for purification of aav vectors by anion exchange chromatography
JP2024503234A (ja)	2020-12-23	2024-01-25	ファイザー・インク	親和性クロマトグラフィーによるａａｖベクターの精製方法
US11760788B2 (en)	2021-03-02	2023-09-19	Pathways Neuro Pharma, Inc.	Neuroreceptor compositions and methods of use
EP4329820A1 (de)	2021-04-26	2024-03-06	Alexion Pharma International Operations Limited	Adeno-assoziierte virale vektorkapside mit verbessertem gewebetropismus
CN113308492B (zh) *	2021-05-31	2023-03-21	北京中因科技有限公司	一种用于复制性aav的检测方法
WO2023114816A1 (en)	2021-12-14	2023-06-22	Neurogene, Inc.	Recombinant optimized galc constructs and methods for treating galc-associated disorders
GB202201242D0 (en)	2022-01-31	2022-03-16	Univ Edinburgh	Recombinant optimized mecp2 cassettes and methods for treating rett syndrome and related disorders
GB202206336D0 (en)	2022-04-29	2022-06-15	Univ Edinburgh	Recombinant therapeutic FMR1 constructs and methods of treating fragile X syndrome and related disorders
WO2024003687A1 (en)	2022-06-28	2024-01-04	Pfizer Inc.	Nucleic acids encoding acid alpha-glucosidase (gaa) and vectors for gene therapy
WO2024038365A1 (en)	2022-08-16	2024-02-22	Pfizer Inc.	Methods for purification of aav vectors by anion exchange chromatography

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US5436146A (en) *	1989-09-07	1995-07-25	The Trustees Of Princeton University	Helper-free stocks of recombinant adeno-associated virus vectors
US5173414A (en) *	1990-10-30	1992-12-22	Applied Immune Sciences, Inc.	Production of recombinant adeno-associated virus vectors
EP0689601B1 (de) *	1993-02-22	2006-10-04	The Rockefeller University	Herstellung von helfer-freien retroviren mit hohem titer mittels transienter transfektion

1996
- 1996-06-06 DE DE69627532T patent/DE69627532T2/de not_active Expired - Lifetime
- 1996-06-06 AU AU60985/96A patent/AU707862B2/en not_active Expired
- 1996-06-06 JP JP50173097A patent/JP4108746B2/ja not_active Expired - Lifetime
- 1996-06-06 EP EP96918295A patent/EP0871483B1/de not_active Expired - Lifetime
- 1996-06-06 AT AT96918295T patent/ATE237352T1/de not_active IP Right Cessation
- 1996-06-06 CA CA2221293A patent/CA2221293C/en not_active Expired - Lifetime
- 1996-06-06 WO PCT/US1996/009405 patent/WO1996040240A1/en active IP Right Grant

Also Published As

Publication number	Publication date
AU6098596A (en)	1996-12-30
EP0871483B1 (de)	2003-04-16
EP0871483A1 (de)	1998-10-21
DE69627532D1 (de)	2003-05-22
EP0871483A4 (de)	2000-01-05
JP4108746B2 (ja)	2008-06-25
CA2221293A1 (en)	1996-12-19
CA2221293C (en)	2012-09-18
AU707862B2 (en)	1999-07-22
WO1996040240A1 (en)	1996-12-19
JPH11510371A (ja)	1999-09-14
ATE237352T1 (de)	2003-05-15

Publication	Publication Date	Title
DE69627532T2 (de)	2004-01-29	Helfervirus-freie herstellung von aav
FI871908A0 (fi)	1987-04-29	Transformering av trichoderma-moegel.
DE69332345D1 (de)	2002-10-31	Herstellung von Brillenlinsen
ATE328068T1 (de)	2006-06-15	Papillomavirus vakzine
ES2035920T3 (es)	1993-05-01	Aerolizacion de agente proteico terapeutico.
IL123833A0 (en)	1998-10-30	Vector and method of use for nucleic acid delivery to non-dividing cells
ATE317908T1 (de)	2006-03-15	Verfahren zur herstellung von rekombinanten viren
TR199501134A2 (tr)	1996-06-21	Memeli ve viral proteinleri asiri ifade edilmesi.
DE69001275D1 (de)	1993-05-13	Satz zur herstellung und abscheidung von endothelzellen.
ES8602768A1 (es)	1985-11-16	Un procedimiento para la preparacion de nuevas n-(heterociclil biciclo)-4-piperidinaminas.
DE69408679D1 (de)	1998-04-02	Herstellung von geformten faserstrukturen
DE69001551D1 (de)	1993-06-17	Latexfilme von naturkautschuk von modifizierter zugfestigkeit.
ATE124086T1 (de)	1995-07-15	Herstellung von proteinen.
DE69024074D1 (de)	1996-01-25	Herstellung von Aramidfasern
DE69033688D1 (de)	2001-02-15	Verwendung härtbarer Silikonkautschukzusammensetzung zur Herstellung von Rollen für Trockenkopiergeräte und Rollen für Trockenkopiergeräte
ATE158184T1 (de)	1997-10-15	Herstellung und verwendung von transfer-faktor
ATE112317T1 (de)	1994-10-15	Bcrf1-proteine als inhibitoren von interferon- g(g).
DE3382072D1 (de)	1991-02-07	Herstellung von herpes-simplex-virusproteinen.
ATE45675T1 (de)	1989-09-15	Verwendung von interferon gamma (ifn-gamma) zur herstellung von praeparationen zur behandlung rheumatischer erkrankungen.
DE3886367D1 (de)	1994-01-27	Verwendung von Antigestagenen zur Herstellung von Arzneimitteln.
ATE96169T1 (de)	1993-11-15	Rekombinante wirte, deren herstellung und verwendung.
SU888095A1 (ru)	1981-12-07	Регулируемый источник посто нного тока
DE3881953D1 (de)	1993-07-29	Expression von fremden genen in drosophila-zellen.
DE69307083D1 (de)	1997-02-13	Verbesserungen in/oder bezüglich der Herstellung von ophtalmischen Linsen
ATE62019T1 (de)	1991-04-15	Verfahren zur herstellung von hcmvglykoproteinen, antikoerper dagegen und hcmvvakzine und dazu zu verwendende rekombinante vektoren.

Legal Events

Date	Code	Title	Description
2004-05-13	8364	No opposition during term of opposition