DE69427876D1 - Methode zur detektion einer gezielten nukleinsäure - Google Patents
Methode zur detektion einer gezielten nukleinsäureInfo
- Publication number
- DE69427876D1 DE69427876D1 DE69427876T DE69427876T DE69427876D1 DE 69427876 D1 DE69427876 D1 DE 69427876D1 DE 69427876 T DE69427876 T DE 69427876T DE 69427876 T DE69427876 T DE 69427876T DE 69427876 D1 DE69427876 D1 DE 69427876D1
- Authority
- DE
- Germany
- Prior art keywords
- nucleic acid
- primer
- detecting
- target nucleic
- targeted nucleic
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 238000000034 method Methods 0.000 title abstract 4
- 108020004707 nucleic acids Proteins 0.000 title abstract 4
- 150000007523 nucleic acids Chemical class 0.000 title abstract 4
- 102000039446 nucleic acids Human genes 0.000 title abstract 4
- 230000037452 priming Effects 0.000 abstract 3
- 108091034117 Oligonucleotide Proteins 0.000 abstract 2
- 230000003321 amplification Effects 0.000 abstract 2
- 238000003199 nucleic acid amplification method Methods 0.000 abstract 2
- 239000002773 nucleotide Substances 0.000 abstract 2
- 125000003729 nucleotide group Chemical group 0.000 abstract 2
- 230000000295 complement effect Effects 0.000 abstract 1
- 238000012544 monitoring process Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| BR9408578A BR9408578A (pt) | 1994-05-23 | 1994-05-23 | Método para detecção de um ácido nucléico alvo |
| PCT/US1994/005767 WO1995032306A1 (en) | 1994-05-23 | 1994-05-23 | Method for detecting a target nucleic acid |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE69427876D1 true DE69427876D1 (de) | 2001-09-06 |
| DE69427876T2 DE69427876T2 (de) | 2002-04-11 |
Family
ID=40549889
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE69427876T Expired - Fee Related DE69427876T2 (de) | 1994-05-23 | 1994-05-23 | Methode zur detektion einer gezielten nukleinsäure |
Country Status (13)
| Country | Link |
|---|---|
| EP (1) | EP0763133B1 (de) |
| JP (1) | JPH10500572A (de) |
| AT (1) | ATE203775T1 (de) |
| AU (1) | AU706033B2 (de) |
| BR (1) | BR9408578A (de) |
| DE (1) | DE69427876T2 (de) |
| DK (1) | DK0763133T3 (de) |
| ES (1) | ES2158895T3 (de) |
| GR (1) | GR3037030T3 (de) |
| HK (1) | HK1001131A1 (de) |
| NZ (1) | NZ266724A (de) |
| RU (1) | RU2158765C2 (de) |
| WO (1) | WO1995032306A1 (de) |
Families Citing this family (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5935522A (en) * | 1990-06-04 | 1999-08-10 | University Of Utah Research Foundation | On-line DNA analysis system with rapid thermal cycling |
| US7273749B1 (en) | 1990-06-04 | 2007-09-25 | University Of Utah Research Foundation | Container for carrying out and monitoring biological processes |
| US7081226B1 (en) | 1996-06-04 | 2006-07-25 | University Of Utah Research Foundation | System and method for fluorescence monitoring |
| US5547861A (en) | 1994-04-18 | 1996-08-20 | Becton, Dickinson And Company | Detection of nucleic acid amplification |
| ES2243393T3 (es) | 1996-06-04 | 2005-12-01 | University Of Utah Research Foundation | Monitorizacion de la hibridacion durante la pcr. |
| JP4281877B2 (ja) * | 1996-06-04 | 2009-06-17 | ユニバーシティ オブ ユタ リサーチ ファウンデーション | 生物学的プロセスを実行し且つモニタリングするためのシステムと方法 |
| US6117635A (en) * | 1996-07-16 | 2000-09-12 | Intergen Company | Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon |
| US5866336A (en) * | 1996-07-16 | 1999-02-02 | Oncor, Inc. | Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon |
| JP4185185B2 (ja) * | 1997-05-08 | 2008-11-26 | 征夫 軽部 | 部分二重鎖ｄｎａを利用したｄｎａの検出方法 |
| WO1999042615A1 (en) * | 1998-02-18 | 1999-08-26 | Dade Behring Inc. | Methods for determining amounts of nucleic acids |
| US20080248536A1 (en) * | 2004-01-23 | 2008-10-09 | Lingvitae As | Polynucleotide Ligation Reactions |
| US9938641B2 (en) | 2006-12-18 | 2018-04-10 | Fluidigm Corporation | Selection of aptamers based on geometry |
| EP2336354A1 (de) * | 2009-12-18 | 2011-06-22 | Roche Diagnostics GmbH | Verfahren für den Nachweis eines RNA-Moleküls, Kit und entsprechendes Verfahren |
| RU2578009C2 (ru) * | 2013-05-08 | 2016-03-20 | Закрытое акционерное общество "ЕВРОГЕН" | Способ идентификации нативных пар фрагментов днк или рнк, присутствующих в одних и тех же живых клетках |
| RU2600873C1 (ru) * | 2015-06-24 | 2016-10-27 | Закрытое акционерное общество "ЕВРОГЕН" | Способ идентификации нативных пар фрагментов днк или рнк, присутствующих в одних и тех же живых клетках |
| KR102468839B1 (ko) * | 2020-06-03 | 2022-11-18 | 주식회사 제노헬릭스 | Rna 검출 방법 |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0232967B1 (de) * | 1986-01-10 | 1993-04-28 | Amoco Corporation | Kompetitiver homogener Test |
| EP0439182B1 (de) * | 1990-01-26 | 1996-04-24 | Abbott Laboratories | Verbessertes Verfahren zur Amplifikation von Nuklein säurezielsequenz, einsetzbar für die Polymerase und Ligasekettenreaktion |
| US5427930A (en) * | 1990-01-26 | 1995-06-27 | Abbott Laboratories | Amplification of target nucleic acids using gap filling ligase chain reaction |
| US5210015A (en) * | 1990-08-06 | 1993-05-11 | Hoffman-La Roche Inc. | Homogeneous assay system using the nuclease activity of a nucleic acid polymerase |
| US5348853A (en) * | 1991-12-16 | 1994-09-20 | Biotronics Corporation | Method for reducing non-specific priming in DNA amplification |
| AU3942993A (en) * | 1992-03-31 | 1993-11-08 | Abbott Laboratories | Method of multiplex ligase chain reaction |
| AU4788493A (en) * | 1992-07-21 | 1994-02-14 | Imclone Systems Incorporated | Gap-filling nucleic acid amplification and detection |
| US6180338B1 (en) * | 1992-08-04 | 2001-01-30 | Beckman Coulter, Inc. | Method, reagent and kit for the detection and amplification of nucleic acid sequences |
-
1994
- 1994-05-23 EP EP94916811A patent/EP0763133B1/de not_active Expired - Lifetime
- 1994-05-23 NZ NZ266724A patent/NZ266724A/en unknown
- 1994-05-23 BR BR9408578A patent/BR9408578A/pt not_active IP Right Cessation
- 1994-05-23 JP JP7530249A patent/JPH10500572A/ja not_active Ceased
- 1994-05-23 DE DE69427876T patent/DE69427876T2/de not_active Expired - Fee Related
- 1994-05-23 DK DK94916811T patent/DK0763133T3/da active
- 1994-05-23 ES ES94916811T patent/ES2158895T3/es not_active Expired - Lifetime
- 1994-05-23 RU RU96124092/14A patent/RU2158765C2/ru active
- 1994-05-23 AT AT94916811T patent/ATE203775T1/de not_active IP Right Cessation
- 1994-05-23 WO PCT/US1994/005767 patent/WO1995032306A1/en active IP Right Grant
- 1994-05-23 AU AU68364/94A patent/AU706033B2/en not_active Ceased
-
1997
- 1997-12-31 HK HK97102691A patent/HK1001131A1/xx not_active IP Right Cessation
-
2001
- 2001-10-26 GR GR20010401900T patent/GR3037030T3/el not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| AU706033B2 (en) | 1999-06-10 |
| BR9408578A (pt) | 1997-08-19 |
| JPH10500572A (ja) | 1998-01-20 |
| ES2158895T3 (es) | 2001-09-16 |
| EP0763133A4 (de) | 1999-02-10 |
| RU2158765C2 (ru) | 2000-11-10 |
| EP0763133B1 (de) | 2001-08-01 |
| AU6836494A (en) | 1995-12-18 |
| EP0763133A1 (de) | 1997-03-19 |
| DE69427876T2 (de) | 2002-04-11 |
| NZ266724A (en) | 1998-08-26 |
| HK1001131A1 (en) | 1998-05-29 |
| DK0763133T3 (da) | 2001-10-08 |
| ATE203775T1 (de) | 2001-08-15 |
| GR3037030T3 (en) | 2002-01-31 |
| WO1995032306A1 (en) | 1995-11-30 |
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| ES2129365A1 (es) | Metodo para la deteccion de secuencias especificas de acidos nucleicos en presencia de un vector util como control positivo interno. |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8364 | No opposition during term of opposition | ||
| 8328 | Change in the person/name/address of the agent |
Representative=s name: GROSSE, BOCKHORNI, SCHUMACHER, 81476 MUENCHEN |
|
| 8339 | Ceased/non-payment of the annual fee |