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DE69233035D1 - Gezielte punkt-mutagenese von dna - Google Patents

Gezielte punkt-mutagenese von dna

Info

Publication number
DE69233035D1
DE69233035D1 DE69233035T DE69233035T DE69233035D1 DE 69233035 D1 DE69233035 D1 DE 69233035D1 DE 69233035 T DE69233035 T DE 69233035T DE 69233035 T DE69233035 T DE 69233035T DE 69233035 D1 DE69233035 D1 DE 69233035D1
Authority
DE
Germany
Prior art keywords
dna
mutant
site
parental
circular
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69233035T
Other languages
English (en)
Inventor
Jac A Nickoloff
Frank A Ray
Win P Deng
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Harvard University
Original Assignee
Harvard University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Harvard University filed Critical Harvard University
Application granted granted Critical
Publication of DE69233035D1 publication Critical patent/DE69233035D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids

Landscapes

  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
DE69233035T 1991-12-24 1992-12-17 Gezielte punkt-mutagenese von dna Expired - Lifetime DE69233035D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US81358191A 1991-12-24 1991-12-24
PCT/US1992/011076 WO1993013216A1 (en) 1991-12-24 1992-12-17 Site-directed mutagenesis of dna

Publications (1)

Publication Number Publication Date
DE69233035D1 true DE69233035D1 (de) 2003-06-05

Family

ID=25212814

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69233035T Expired - Lifetime DE69233035D1 (de) 1991-12-24 1992-12-17 Gezielte punkt-mutagenese von dna

Country Status (7)

Country Link
US (1) US5354670A (de)
EP (1) EP0620858B1 (de)
JP (1) JP3398957B2 (de)
AT (1) ATE239089T1 (de)
CA (1) CA2126438C (de)
DE (1) DE69233035D1 (de)
WO (1) WO1993013216A1 (de)

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US9150852B2 (en) 2011-02-18 2015-10-06 Raindance Technologies, Inc. Compositions and methods for molecular labeling
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WO2012167382A1 (en) 2011-06-08 2012-12-13 Children's Hospital Of Eastern Ontario Research Institute Inc. Compositions and methods for glioblastoma treatment
US8658430B2 (en) 2011-07-20 2014-02-25 Raindance Technologies, Inc. Manipulating droplet size
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WO2013109950A1 (en) * 2012-01-20 2013-07-25 Kennesaw State University Research And Services Foundation Methods to mutate circular deoxyribonucleotides
AU2013232733B2 (en) 2012-03-13 2017-01-19 James Cook University Method for treating inflammation
WO2014071182A1 (en) 2012-11-01 2014-05-08 Massachusetts Institute Of Technology Directed evolution of synthetic gene cluster
JP2016513115A (ja) 2013-02-21 2016-05-12 チルドレンズ ホスピタル オブ イースタン オンタリオ リサーチ インスティチュート インコーポレイテッド ワクチン組成物
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CA2924130A1 (en) 2013-09-18 2015-03-26 James Cook University Anti-inflammatory proteins and methods of use
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CN108602729B (zh) 2015-07-13 2022-07-05 皮沃特生物公司 改良植物性状的方法及组合物
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CN114555833A (zh) * 2019-08-16 2022-05-27 分析生物科学有限公司 用于确定t细胞库的靶向杂交捕获方法
WO2021221690A1 (en) 2020-05-01 2021-11-04 Pivot Bio, Inc. Modified bacterial strains for improved fixation of nitrogen
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BR112022022714A2 (pt) 2020-05-13 2023-03-28 Pivot Bio Inc Bactéria diazotrófica gram-positiva geneticamente modificada, composição microbiana, método para fornecer nitrogênio fixo a uma planta, gene glnr, proteína glnr, e, método para identificar reguladores de um operon nif
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Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2092527T3 (es) * 1990-06-08 1996-12-01 Behringwerke Ag Metodo para la determinacion cuantitativa de secuencias de adn.
DE4024187A1 (de) * 1990-07-30 1992-02-06 Biotechnolog Forschung Gmbh Oligonucleotid-gerichtete mutagenese von plasmiden

Also Published As

Publication number Publication date
JP3398957B2 (ja) 2003-04-21
EP0620858A4 (en) 1997-05-07
ATE239089T1 (de) 2003-05-15
CA2126438C (en) 2003-12-02
JPH08500721A (ja) 1996-01-30
WO1993013216A1 (en) 1993-07-08
CA2126438A1 (en) 1993-07-08
US5354670A (en) 1994-10-11
EP0620858B1 (de) 2003-05-02
EP0620858A1 (de) 1994-10-26

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