[go: up one dir, main page]

DE69127657D1 - Proteinreinigungsmethode - Google Patents

Proteinreinigungsmethode

Info

Publication number
DE69127657D1
DE69127657D1 DE69127657T DE69127657T DE69127657D1 DE 69127657 D1 DE69127657 D1 DE 69127657D1 DE 69127657 T DE69127657 T DE 69127657T DE 69127657 T DE69127657 T DE 69127657T DE 69127657 D1 DE69127657 D1 DE 69127657D1
Authority
DE
Germany
Prior art keywords
protein
purification method
protein purification
antibodies
ion exchange
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Revoked
Application number
DE69127657T
Other languages
English (en)
Other versions
DE69127657T2 (de
Inventor
Michael H Coan
Vivian W Lee
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bayer AG
Bayer Corp
Original Assignee
Bayer AG
Bayer Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=24106028&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DE69127657(D1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Bayer AG, Bayer Corp filed Critical Bayer AG
Application granted granted Critical
Publication of DE69127657D1 publication Critical patent/DE69127657D1/de
Publication of DE69127657T2 publication Critical patent/DE69127657T2/de
Anticipated expiration legal-status Critical
Revoked legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
    • C07K16/065—Purification, fragmentation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/24—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against cytokines, lymphokines or interferons
    • C07K16/241—Tumor Necrosis Factors

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Peptides Or Proteins (AREA)
DE69127657T 1990-05-25 1991-05-11 Proteinreinigungsmethode Revoked DE69127657T2 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/528,523 US5110913A (en) 1990-05-25 1990-05-25 Antibody purification method

Publications (2)

Publication Number Publication Date
DE69127657D1 true DE69127657D1 (de) 1997-10-23
DE69127657T2 DE69127657T2 (de) 1998-01-15

Family

ID=24106028

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69127657T Revoked DE69127657T2 (de) 1990-05-25 1991-05-11 Proteinreinigungsmethode

Country Status (9)

Country Link
US (1) US5110913A (de)
EP (1) EP0460426B1 (de)
JP (1) JP3262128B2 (de)
AT (1) ATE158302T1 (de)
CA (1) CA2042945C (de)
DE (1) DE69127657T2 (de)
DK (1) DK0460426T3 (de)
ES (1) ES2106742T3 (de)
GR (1) GR3025504T3 (de)

Families Citing this family (35)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5733742A (en) * 1993-06-03 1998-03-31 Therapeutic Antibodies Inc. Production of antibody fragments from whole blood
US5759864A (en) * 1995-06-23 1998-06-02 Cedars Sinai Medical Center Methods for reducing background binding in antibody preparations
TW505655B (en) * 1997-10-14 2002-10-11 Tanox Inc Enhanced aggregate removal from bulk-biologicals using ion exchange chromatography
EP1308456B1 (de) * 1998-05-06 2007-08-22 Genentech, Inc. Reinigung von Antikörpern durch Ionenaustauschchromatographie
CN103641885A (zh) 1998-05-06 2014-03-19 基因技术股份有限公司 用离子交换层析纯化蛋白质
JP2002517406A (ja) 1998-06-01 2002-06-18 ジェネンテック・インコーポレーテッド イオン交換クロマトグラフィーの使用による、凝集体からのタンパク質モノマーの分離
WO2004050683A2 (en) * 2002-12-02 2004-06-17 Abgenix, Inc. Antibodies directed to tumor necrosis factor and uses thereof
US7662930B2 (en) * 2005-12-06 2010-02-16 Amgen Inc. Polishing steps used in multi-step protein purification processes
US7863426B2 (en) 2006-04-05 2011-01-04 Abbott Biotechnology Ltd. Antibody purification
US8911964B2 (en) 2006-09-13 2014-12-16 Abbvie Inc. Fed-batch method of making human anti-TNF-alpha antibody
AU2007294731B2 (en) 2006-09-13 2014-04-17 Abbvie Inc. Cell culture improvements
CA2666607C (en) * 2006-11-01 2015-02-10 Biogen Idec Ma Inc. Method of isolating biomacromolecules using low ph and divalent cations
PT2215117E (pt) 2007-10-30 2015-04-01 Genentech Inc Purificação de anticorpo por cromatografia de troca de catiões
DK2288358T3 (en) * 2008-04-16 2016-01-11 Biogen Ma Inc A method of isolating antibodies using polyalkylene glycol and transition metals
SG195577A1 (en) 2008-10-20 2013-12-30 Abbott Lab Viral inactivation during purification of antibodies
TW201024318A (en) 2008-10-20 2010-07-01 Abbott Lab Isolation and purification of antibodies using protein A affinity chromatography
EP2702077A2 (de) 2011-04-27 2014-03-05 AbbVie Inc. Verfahren zur steuerung des galactosylierungsprofil von rekombinant exprimierten proteinen
US9067990B2 (en) 2013-03-14 2015-06-30 Abbvie, Inc. Protein purification using displacement chromatography
WO2013158273A1 (en) 2012-04-20 2013-10-24 Abbvie Inc. Methods to modulate c-terminal lysine variant distribution
US9334319B2 (en) 2012-04-20 2016-05-10 Abbvie Inc. Low acidic species compositions
US9505833B2 (en) 2012-04-20 2016-11-29 Abbvie Inc. Human antibodies that bind human TNF-alpha and methods of preparing the same
US9249182B2 (en) 2012-05-24 2016-02-02 Abbvie, Inc. Purification of antibodies using hydrophobic interaction chromatography
US9512214B2 (en) 2012-09-02 2016-12-06 Abbvie, Inc. Methods to control protein heterogeneity
WO2014035475A1 (en) 2012-09-02 2014-03-06 Abbvie Inc. Methods to control protein heterogeneity
US10023608B1 (en) 2013-03-13 2018-07-17 Amgen Inc. Protein purification methods to remove impurities
EP2970375A1 (de) * 2013-03-14 2016-01-20 AbbVie Inc. Schwach saure zusammensetzungen und verfahren zu ihrer herstellung durch verdrängungschromatografie
US8921526B2 (en) 2013-03-14 2014-12-30 Abbvie, Inc. Mutated anti-TNFα antibodies and methods of their use
US9017687B1 (en) 2013-10-18 2015-04-28 Abbvie, Inc. Low acidic species compositions and methods for producing and using the same using displacement chromatography
US9499614B2 (en) 2013-03-14 2016-11-22 Abbvie Inc. Methods for modulating protein glycosylation profiles of recombinant protein therapeutics using monosaccharides and oligosaccharides
US9598667B2 (en) 2013-10-04 2017-03-21 Abbvie Inc. Use of metal ions for modulation of protein glycosylation profiles of recombinant proteins
US9181337B2 (en) 2013-10-18 2015-11-10 Abbvie, Inc. Modulated lysine variant species compositions and methods for producing and using the same
US8946395B1 (en) 2013-10-18 2015-02-03 Abbvie Inc. Purification of proteins using hydrophobic interaction chromatography
US9085618B2 (en) 2013-10-18 2015-07-21 Abbvie, Inc. Low acidic species compositions and methods for producing and using the same
US20150139988A1 (en) 2013-11-15 2015-05-21 Abbvie, Inc. Glycoengineered binding protein compositions
CN105315369B (zh) * 2014-07-25 2020-03-13 山东博安生物技术有限公司 利用阳离子交换层析纯化蛋白质

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6019733B2 (ja) * 1977-06-10 1985-05-17 科研製薬株式会社 蛋白質性活性物質
DE3526096A1 (de) * 1985-07-22 1987-01-22 Basf Ag Verfahren zur reinigung von htnf
AT399095B (de) * 1986-03-27 1995-03-27 Vukovich Thomas Dr Verfahren zur auftrennung von proteinen mittels gradientenelution und vorrichtung zur durchführung des verfahrens
US4771128A (en) * 1986-10-10 1988-09-13 Cetus Corporation Method of purifying toxin conjugates using hydrophobic interaction chromatography
EP0391934A1 (de) * 1987-10-23 1990-10-17 Schering Corporation Verfahren zur proteinreinigung
JPH01247096A (ja) * 1988-03-30 1989-10-02 Mitsubishi Petrochem Co Ltd モノクローナル抗体の回収方法

Also Published As

Publication number Publication date
GR3025504T3 (en) 1998-02-27
CA2042945C (en) 2004-05-18
EP0460426B1 (de) 1997-09-17
ATE158302T1 (de) 1997-10-15
US5110913A (en) 1992-05-05
DK0460426T3 (da) 1998-05-04
DE69127657T2 (de) 1998-01-15
EP0460426A2 (de) 1991-12-11
JP3262128B2 (ja) 2002-03-04
ES2106742T3 (es) 1997-11-16
JPH06316593A (ja) 1994-11-15
EP0460426A3 (en) 1992-07-22
CA2042945A1 (en) 1991-11-26

Similar Documents

Publication Publication Date Title
DE69127657D1 (de) Proteinreinigungsmethode
Porath Salt-promoted adsorption: recent developments
Porath Cross-linked dextrans as molecular sieves
NZ507557A (en) Protein purification by ion exchange chromatography
ATE179425T1 (de) Verfahren zur abreicherung oder entfernung von endotoxinen
DE69113872D1 (de) Biologisch aktive Reagenzien aus Carboxylgruppenhaltigen Polymerisaten, analytisches Element und Verfahren zur Verwendung.
MX9707909A (es) Purificacion de anticuerpos.
ES2112865T3 (es) Anticuerpos igg purificados.
MX9603637A (es) Purificacion de anticuerpo.
DE68915675D1 (de) Verfahren zur Reinigung von Vitamin K-abhängigen Proteinen.
DK0783366T3 (da) Kromatografiske harpikser og fremgangsmåder til anvendelse af samme
DK0930366T3 (da) Bindingsdomæner i Delta-proteiner
DE69927013D1 (de) Effiziente reinigung von adenovirus
DE59008334D1 (de) Verfahren zur Präparierung von biologischem Implantationsmaterial.
DK564989D0 (da) Bindingsproteiner
ATE121752T1 (de) Entfernung von protein a aus antikörper- präparationen.
GB1447074A (en) Method for separating a polypeptide from microorganisms
ATE124703T1 (de) Verfahren zur trennung von proteinen.
ATE111519T1 (de) Familie von modifizierten antikörpern mit hoher affinität zur behandlung von krebs.
Borrebaeck et al. Metal chelate affinity chromatography of the Dolichos biflorus seed lectin and its subunits
Ramadan et al. α-Aminoacyl hydroxamate adsorbents—a new type of immobilized chelator
DE69012734D1 (de) Verfahren zum Eluieren von adsorbiertem Gold aus Kohlenstoff.
EA199700196A1 (ru) Способ получения фактора ix из биологических источников и фактор ix, полученный этим способом
DK510084A (da) Uoploeselige overflader behandlet til haemning af ikke-specifik proteinbinding
SE9201976D0 (sv) En metod foer att adsorbera proteiner och peptider till hydrofoba zeoliter

Legal Events

Date Code Title Description
8363 Opposition against the patent
8331 Complete revocation