DE69031318D1 - Verfahren und Vorrichtung zur Hybridisierung von Nukleinsäure mit verbesserter Reaktionskinetik - Google Patents
Verfahren und Vorrichtung zur Hybridisierung von Nukleinsäure mit verbesserter ReaktionskinetikInfo
- Publication number
- DE69031318D1 DE69031318D1 DE69031318T DE69031318T DE69031318D1 DE 69031318 D1 DE69031318 D1 DE 69031318D1 DE 69031318 T DE69031318 T DE 69031318T DE 69031318 T DE69031318 T DE 69031318T DE 69031318 D1 DE69031318 D1 DE 69031318D1
- Authority
- DE
- Germany
- Prior art keywords
- zone
- chromatographic
- solvent transport
- capture
- chromatographic solvent
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 238000000034 method Methods 0.000 title abstract 2
- 108020004707 nucleic acids Proteins 0.000 title abstract 2
- 150000007523 nucleic acids Chemical class 0.000 title abstract 2
- 102000039446 nucleic acids Human genes 0.000 title abstract 2
- 239000002904 solvent Substances 0.000 abstract 7
- 239000000523 sample Substances 0.000 abstract 4
- 238000009396 hybridization Methods 0.000 abstract 2
- 108020004635 Complementary DNA Proteins 0.000 abstract 1
- 239000003153 chemical reaction reagent Substances 0.000 abstract 1
- 230000002452 interceptive effect Effects 0.000 abstract 1
- 239000000463 material Substances 0.000 abstract 1
- 238000007899 nucleic acid hybridization Methods 0.000 abstract 1
- 239000002773 nucleotide Substances 0.000 abstract 1
- 125000003729 nucleotide group Chemical group 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
- C12Q1/701—Specific hybridization probes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
- B01L2400/0406—Moving fluids with specific forces or mechanical means specific forces capillary forces
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Immunology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Genetics & Genomics (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Virology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US32492689A | 1989-03-17 | 1989-03-17 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE69031318D1 true DE69031318D1 (de) | 1997-10-02 |
Family
ID=23265712
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE69031318T Expired - Lifetime DE69031318D1 (de) | 1989-03-17 | 1990-03-08 | Verfahren und Vorrichtung zur Hybridisierung von Nukleinsäure mit verbesserter Reaktionskinetik |
Country Status (7)
| Country | Link |
|---|---|
| EP (1) | EP0387696B1 (de) |
| JP (1) | JPH02283299A (de) |
| KR (1) | KR900014882A (de) |
| AT (1) | ATE157404T1 (de) |
| AU (1) | AU636875B2 (de) |
| CA (1) | CA2012355A1 (de) |
| DE (1) | DE69031318D1 (de) |
Families Citing this family (31)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5340728A (en) * | 1992-12-09 | 1994-08-23 | E. I. Du Pont De Nemours And Company | Method for amplification of targeted segments of nucleic acid using nested polymerase chain reaction |
| WO1995011454A1 (en) * | 1993-10-21 | 1995-04-27 | Abbott Laboratories | Apparatus and method for detecting a target ligand |
| WO1995027081A1 (en) * | 1994-03-31 | 1995-10-12 | E.I. Du Pont De Nemours And Company | A method for detection of nucleic acid fragments |
| US6037127A (en) * | 1994-03-31 | 2000-03-14 | E. I. Du Pont De Nemours And Company | Method for detection of non-denatured nucleic acid fragments |
| US6852487B1 (en) | 1996-02-09 | 2005-02-08 | Cornell Research Foundation, Inc. | Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays |
| EP0912761A4 (de) | 1996-05-29 | 2004-06-09 | Cornell Res Foundation Inc | Bestimmung von unterschieden in der nukleisäuresequenz mittels einer kombination von ligasebestimmung und polymerase-kettenreaktion |
| US6337214B1 (en) | 1997-06-09 | 2002-01-08 | Acgt Medico, Inc. | Detection of DNA, RNA and proteins using a test column with two snares |
| GB9711941D0 (en) | 1997-06-09 | 1997-08-06 | Acgt Medico Inc | Detection of pathogens |
| WO1999042620A2 (en) * | 1998-02-19 | 1999-08-26 | Edvotek | Articles of manufacture and methods for staining biomolecules |
| EP1084278B1 (de) * | 1998-06-08 | 2005-10-19 | ACGT Medico Inc. | Erkennung von dns, rns und proteinen |
| GB9821526D0 (en) * | 1998-10-02 | 1998-11-25 | Genosis Inc | Capture assay |
| ATE273516T1 (de) | 1999-06-23 | 2004-08-15 | Cornell Res Foundation Inc | Entwässerung/rehydratisierung von markierten liposomen auf einer prüfeinrichtung |
| US6576460B1 (en) | 1999-10-28 | 2003-06-10 | Cornell Research Foundation, Inc. | Filtration-detection device and method of use |
| EP1303639A2 (de) | 2000-04-14 | 2003-04-23 | Cornell Research Foundation, Inc. | DESIGNMETHODE FüR EINEN ADRESSIERBARER ARRAY ZUR DETEKTION VON NUCLEINSäURE SEQUENZUNTERSCHIEDEN UNTER VERWENDUNG EINER LIGASE DETEKTIONSREAKTION |
| GB0016836D0 (en) | 2000-07-07 | 2000-08-30 | Lee Helen | Improved dipstick assays (1) |
| GB0016813D0 (en) | 2000-07-07 | 2000-08-30 | Lee Helen | Improved dipstick assays (4) |
| DE60318435T2 (de) | 2002-05-31 | 2008-12-24 | Cornell Research Foundation, Inc. | Universeller biosensor und verfahren zur verwendung |
| US7920906B2 (en) | 2005-03-10 | 2011-04-05 | Dexcom, Inc. | System and methods for processing analyte sensor data for sensor calibration |
| US9247900B2 (en) | 2004-07-13 | 2016-02-02 | Dexcom, Inc. | Analyte sensor |
| US20070045902A1 (en) | 2004-07-13 | 2007-03-01 | Brauker James H | Analyte sensor |
| US7654956B2 (en) | 2004-07-13 | 2010-02-02 | Dexcom, Inc. | Transcutaneous analyte sensor |
| GB0701253D0 (en) | 2007-01-23 | 2007-02-28 | Diagnostics For The Real World | Nucleic acid amplification and testing |
| WO2012070618A1 (ja) * | 2010-11-24 | 2012-05-31 | 株式会社カネカ | 増幅核酸検出方法及び検出デバイス |
| JP2013198482A (ja) * | 2012-02-22 | 2013-10-03 | Nippon Meat Packers Inc | 核酸の検出方法 |
| EP2843058B1 (de) | 2012-04-27 | 2019-12-18 | Kaneka Corporation | Verfahren zur nukleinsäureamplifikation und verfahren zum nachweis von amplifizierten nukleinsäuren |
| JP6151695B2 (ja) * | 2012-07-17 | 2017-06-21 | 日本碍子株式会社 | 核酸クロマトグラフィー検査具及びその製造方法 |
| WO2014070460A1 (en) * | 2012-10-31 | 2014-05-08 | Cellay, Inc. | Methods and kits for performing in situ hybridization |
| WO2015076356A1 (ja) | 2013-11-22 | 2015-05-28 | 株式会社カネカ | 短鎖ｒｎａの検出方法 |
| JP6150918B2 (ja) * | 2016-03-03 | 2017-06-21 | 日本碍子株式会社 | 核酸クロマトグラフィー検査具 |
| CN114350753A (zh) * | 2021-12-20 | 2022-04-15 | 江苏海洋大学 | 自由联结的管式基因芯片及其在疾病检测中的应用 |
| CN114231401B (zh) * | 2022-02-21 | 2022-05-03 | 北京芯迈微生物技术有限公司 | 一种核酸检测试剂盒及检测方法 |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US731335A (en) * | 1902-09-17 | 1903-06-16 | Nicholas Blanchet | Automatic bait-box. |
| US4623627A (en) * | 1983-08-19 | 1986-11-18 | Cetus Corporation | Monoclonal antibody having specificity for the double-stranded conformation of native DNA and diagnostic methods using same |
| US4652517A (en) * | 1984-06-11 | 1987-03-24 | Diagnostic Research Limited Partnership | Methods for the in vitro detection and identification of unknown pathogens or genetic entities |
| US4731335A (en) * | 1985-09-13 | 1988-03-15 | Fisher Scientific Company | Method for treating thin samples on a surface employing capillary flow |
| US4960691A (en) * | 1986-09-29 | 1990-10-02 | Abbott Laboratories | Chromatographic test strip for determining ligands or receptors |
| EP0366241A3 (de) * | 1988-10-04 | 1990-05-23 | Fisher Scientific Company | Vorrichtung mit adsorbierender Oberfläche und Herstellungsverfahren |
-
1990
- 1990-03-08 EP EP90104413A patent/EP0387696B1/de not_active Expired - Lifetime
- 1990-03-08 AU AU51196/90A patent/AU636875B2/en not_active Ceased
- 1990-03-08 AT AT90104413T patent/ATE157404T1/de not_active IP Right Cessation
- 1990-03-08 DE DE69031318T patent/DE69031318D1/de not_active Expired - Lifetime
- 1990-03-15 JP JP2065501A patent/JPH02283299A/ja active Pending
- 1990-03-15 KR KR1019900003448A patent/KR900014882A/ko not_active Withdrawn
- 1990-03-16 CA CA002012355A patent/CA2012355A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| EP0387696A3 (de) | 1992-04-15 |
| AU636875B2 (en) | 1993-05-13 |
| AU5119690A (en) | 1990-09-20 |
| EP0387696A2 (de) | 1990-09-19 |
| JPH02283299A (ja) | 1990-11-20 |
| KR900014882A (ko) | 1990-10-25 |
| CA2012355A1 (en) | 1990-09-17 |
| EP0387696B1 (de) | 1997-08-27 |
| ATE157404T1 (de) | 1997-09-15 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8332 | No legal effect for de |