DE68923503D1 - Thermostabile DNA-Polymerase und Verfahren zur Herstellung davon. - Google Patents

Thermostabile DNA-Polymerase und Verfahren zur Herstellung davon.

Info

Publication number: DE68923503D1
Authority: DE; Germany
Prior art keywords: preparation; dna polymerase; thermostable dna; thermostable; polymerase
Prior art date: 1988-08-26
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Expired - Lifetime

Application number

DE68923503T

Other languages

English (en)

Other versions

DE68923503T2 (de

Inventor

Tairo Oshima

Hitoshi C O Toyo Bos Sakashita

Hakuji C O Toyo Bose Matsumoto

Yoshihiko C O Toyo Bos Maekawa

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Toyobo Co Ltd

Original Assignee

Toyobo Co Ltd

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1988-08-26

Filing date

1989-08-25

Publication date

1995-08-24

1989-08-25 Application filed by Toyobo Co Ltd filed Critical Toyobo Co Ltd

1995-08-24 Publication of DE68923503D1 publication Critical patent/DE68923503D1/de

1996-02-01 Application granted granted Critical

1996-02-01 Publication of DE68923503T2 publication Critical patent/DE68923503T2/de

2009-08-26 Anticipated expiration legal-status Critical

Status Expired - Lifetime legal-status Critical Current

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
- C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase

Landscapes

Life Sciences & Earth Sciences (AREA)
Health & Medical Sciences (AREA)
Chemical & Material Sciences (AREA)
Wood Science & Technology (AREA)
Molecular Biology (AREA)
Engineering & Computer Science (AREA)
Bioinformatics & Cheminformatics (AREA)
Organic Chemistry (AREA)
Zoology (AREA)
Genetics & Genomics (AREA)
Medicinal Chemistry (AREA)
Microbiology (AREA)
Biotechnology (AREA)
Biochemistry (AREA)
General Engineering & Computer Science (AREA)
General Health & Medical Sciences (AREA)
Biomedical Technology (AREA)
Enzymes And Modification Thereof (AREA)

DE68923503T 1988-08-26 1989-08-25 Thermostabile DNA-Polymerase und Verfahren zur Herstellung davon. Expired - Lifetime DE68923503T2 (de)

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
JP63213330A JP2531246B2 (ja)	1988-08-26	1988-08-26	耐熱性ｄｎａポリメラ―ゼおよびその製法

Publications (2)

Publication Number	Publication Date
DE68923503D1 true DE68923503D1 (de)	1995-08-24
DE68923503T2 DE68923503T2 (de)	1996-02-01

Family

ID=16637372

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
DE68923503T Expired - Lifetime DE68923503T2 (de)	1988-08-26	1989-08-25	Thermostabile DNA-Polymerase und Verfahren zur Herstellung davon.

Country Status (4)

Country	Link
US (2)	US5192674A (de)
EP (1)	EP0359006B1 (de)
JP (1)	JP2531246B2 (de)
DE (1)	DE68923503T2 (de)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US5374553A (en) *	1986-08-22	1994-12-20	Hoffmann-La Roche Inc.	DNA encoding a thermostable nucleic acid polymerase enzyme from thermotoga maritima
US5322770A (en) *	1989-12-22	1994-06-21	Hoffman-Laroche Inc.	Reverse transcription with thermostable DNA polymerases - high temperature reverse transcription
US5693517A (en) *	1987-06-17	1997-12-02	Roche Molecular Systems, Inc.	Reagents and methods for coupled high temperature reverse transcription and polymerase chain reactions
US5407800A (en) *	1986-08-22	1995-04-18	Hoffmann-La Roche Inc.	Reverse transcription with Thermus thermophilus polymerase
US5310652A (en) *	1986-08-22	1994-05-10	Hoffman-La Roche Inc.	Reverse transcription with thermostable DNA polymerase-high temperature reverse transcription
US5618711A (en) *	1986-08-22	1997-04-08	Hoffmann-La Roche Inc.	Recombinant expression vectors and purification methods for Thermus thermophilus DNA polymerase
US5561058A (en) *	1986-08-22	1996-10-01	Hoffmann-La Roche Inc.	Methods for coupled high temperatures reverse transcription and polymerase chain reactions
CA2071196C (en) *	1989-12-22	2002-04-23	David H. Gelfand	Recombinant expression vectors and purification methods for thermus thermophilus dna polymerase
US5648211A (en) *	1994-04-18	1997-07-15	Becton, Dickinson And Company	Strand displacement amplification using thermophilic enzymes
US5602011A (en) *	1995-01-18	1997-02-11	Pharmacia Biotech Inc.	Purified Thermococcus barossii DNA polymerase
US6291164B1 (en)	1996-11-22	2001-09-18	Invitrogen Corporation	Methods for preventing inhibition of nucleic acid synthesis by pyrophosphate
US7556958B1 (en)	1997-04-08	2009-07-07	The Rockefeller University	Enzyme derived from thermophilic organisms that functions as a chromosomal replicase, and preparation and uses thereof
EP1086209B1 (de) *	1998-06-17	2007-08-22	Amersham Biosciences Corp.	Fy7 polymerase
US20030134292A1 (en) *	2001-10-30	2003-07-17	Farchaus Joseph W.	Thermostable DNA polymerases and methods of making same
AU2003209117A1 (en) *	2002-02-12	2003-09-04	Applera Corporation	Polymerase compositions
DE10315640A1 (de) *	2003-04-04	2004-10-14	Ignatov, Konstantin	Verfahren zur kontrollierten Freisetzung von Komponenten in eine Lösung
GB2416352B (en) *	2004-07-21	2009-01-28	Bioline Ltd	A method for performing the hot start of enzymatic reactions
WO2008152102A1 (en) *	2007-06-13	2008-12-18	Ge Healthcare Bio-Sciences Corp	Polymerase stabilization
US20110250598A1 (en)	2010-04-12	2011-10-13	Ulrike Fischer	Detergent free polymerases
JP7610917B2 (ja)	2016-04-14	2025-01-09	ティー２バイオシステムズ，インコーポレーテッド	複合試料における増幅のための方法及びシステム

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
CA1338457C (en) *	1986-08-22	1996-07-16	Henry A. Erlich	Purified thermostable enzyme
ZA876216B (en) *	1986-08-22	1989-04-26	Cetus Corp	Purified thermostable enzyme
US4889818A (en) *	1986-08-22	1989-12-26	Cetus Corporation	Purified thermostable enzyme

1988
- 1988-08-26 JP JP63213330A patent/JP2531246B2/ja not_active Expired - Lifetime
1989
- 1989-08-24 US US07/398,178 patent/US5192674A/en not_active Expired - Lifetime
- 1989-08-25 DE DE68923503T patent/DE68923503T2/de not_active Expired - Lifetime
- 1989-08-25 EP EP89115701A patent/EP0359006B1/de not_active Expired - Lifetime
1992
- 1992-06-05 US US07/894,085 patent/US5413926A/en not_active Expired - Lifetime

Also Published As

Publication number	Publication date
EP0359006B1 (de)	1995-07-19
EP0359006A1 (de)	1990-03-21
US5192674A (en)	1993-03-09
JPH0260585A (ja)	1990-03-01
JP2531246B2 (ja)	1996-09-04
US5413926A (en)	1995-05-09
DE68923503T2 (de)	1996-02-01

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Legal Events

Date	Code	Title	Description
1996-08-14	8364	No opposition during term of opposition