[go: up one dir, main page]

DE68914664D1 - PURIFIED UBIQUITIN HYDROLASE, THESE ENCODING DNA SEQUENCES, AND THEIR USE TO OBTAIN POLYPEPTIDES. - Google Patents

PURIFIED UBIQUITIN HYDROLASE, THESE ENCODING DNA SEQUENCES, AND THEIR USE TO OBTAIN POLYPEPTIDES.

Info

Publication number
DE68914664D1
DE68914664D1 DE68914664T DE68914664T DE68914664D1 DE 68914664 D1 DE68914664 D1 DE 68914664D1 DE 68914664 T DE68914664 T DE 68914664T DE 68914664 T DE68914664 T DE 68914664T DE 68914664 D1 DE68914664 D1 DE 68914664D1
Authority
DE
Germany
Prior art keywords
dna sequences
encoding dna
ubiquitin hydrolase
obtain polypeptides
purified ubiquitin
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE68914664T
Other languages
German (de)
Other versions
DE68914664T2 (en
Inventor
Chung-Cheng Liu
Harvey Miller
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Genentech Inc
Original Assignee
Genentech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US07/210,909 external-priority patent/US5156968A/en
Application filed by Genentech Inc filed Critical Genentech Inc
Application granted granted Critical
Publication of DE68914664D1 publication Critical patent/DE68914664D1/en
Publication of DE68914664T2 publication Critical patent/DE68914664T2/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/64—Relaxins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
    • C12N9/60—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi from yeast
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/95—Fusion polypeptide containing a motif/fusion for degradation (ubiquitin fusions, PEST sequence)

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Wood Science & Technology (AREA)
  • Mycology (AREA)
  • Engineering & Computer Science (AREA)
  • Endocrinology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Toxicology (AREA)
  • General Engineering & Computer Science (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
DE68914664T 1988-06-24 1989-06-16 PURIFIED UBIQUITIN HYDROLASE, THESE ENCODING DNA SEQUENCES, AND THEIR USE TO OBTAIN POLYPEPTIDES. Expired - Lifetime DE68914664T2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US07/210,909 US5156968A (en) 1988-06-24 1988-06-24 Purified yeast ubiquitin hydrolase
US07/284,281 US5108919A (en) 1988-06-24 1988-12-14 Dna sequences encoding yeast ubiquitin hydrolase
PCT/US1989/002651 WO1989012678A1 (en) 1988-06-24 1989-06-16 Purified ubiquitin hydrolase, dna sequences encoding same, and its use in recovering polypeptides

Publications (2)

Publication Number Publication Date
DE68914664D1 true DE68914664D1 (en) 1994-05-19
DE68914664T2 DE68914664T2 (en) 1994-10-20

Family

ID=26905634

Family Applications (1)

Application Number Title Priority Date Filing Date
DE68914664T Expired - Lifetime DE68914664T2 (en) 1988-06-24 1989-06-16 PURIFIED UBIQUITIN HYDROLASE, THESE ENCODING DNA SEQUENCES, AND THEIR USE TO OBTAIN POLYPEPTIDES.

Country Status (9)

Country Link
US (1) US5108919A (en)
EP (1) EP0420936B1 (en)
JP (1) JPH03505527A (en)
AU (1) AU619319B2 (en)
DE (1) DE68914664T2 (en)
DK (1) DK303790A (en)
ES (1) ES2018901A6 (en)
FI (1) FI906214A7 (en)
WO (1) WO1989012678A1 (en)

Families Citing this family (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5196321A (en) * 1986-10-02 1993-03-23 Massachusetts Institute Of Technology Methods for in vitro cleavage of ubiquitin fusion proteins
US5093242A (en) * 1986-10-02 1992-03-03 Massachusetts Institute Of Technology Methods of generating desired amino-terminal residues in proteins
DK0479912T3 (en) * 1989-06-30 1994-10-10 Massachusetts Inst Technology Method for inhibiting the course of N-end rule in living cells
US5620923A (en) * 1989-10-12 1997-04-15 The University Of Utah Synthesis of peptides as cloned ubiquitin extensions
DK0445097T3 (en) * 1990-02-26 1996-09-16 Univ Washington Process for N-myristoylation of protein
US5212058A (en) * 1990-05-09 1993-05-18 Massachusetts Institute Of Technology Nucleic acid encoding ubiquitin-specific proteases
ES2071315T3 (en) * 1990-05-09 1995-06-16 Massachusetts Inst Technology UBIQUITINE-SPECIFIC PROTEASE.
US5716816A (en) * 1992-05-22 1998-02-10 The United States Of America As Represented By The Department Of Health And Human Services Clones encoding mammalian ADP-ribosylarginine hydrolases
ES2174868T3 (en) * 1992-10-29 2002-11-16 Celtrix Pharma FRAGMENT OF THE FIXING RECEIVER TO TGF-BETA TIPOII AS A THERAPEUTIC AGENT.
US6653450B1 (en) 1993-01-28 2003-11-25 Cohesion Technologies, Inc. Mutated recombinant collagens
US5459051A (en) * 1993-03-26 1995-10-17 Celtrix Pharmaceuticals, Inc. Methods and vectors for over-expression of ubiquitin fusion proteins in host cells
US5914254A (en) * 1993-08-02 1999-06-22 Celtrix Pharmaceuticals, Inc. Expression of fusion polypeptides transported out of the cytoplasm without leader sequences
US5563046A (en) * 1993-08-02 1996-10-08 Celtrix Pharmaceuticals, Inc. Fusion polypeptides and proteins
US5981699A (en) * 1994-01-04 1999-11-09 Mitotix, Inc. Human ubiquitin conjugating enzyme
US5744343A (en) * 1994-01-04 1998-04-28 Mitotix, Inc. Ubiquitin conjugating enzymes
EP0738394B1 (en) * 1994-01-04 2000-05-17 Mitotix, Inc. Ubiquitin conjugating enzymes
US5770720A (en) * 1995-08-30 1998-06-23 Barnes-Jewish Hospital Ubiquitin conjugating enzymes having transcriptional repressor activity
US6001619A (en) * 1995-10-04 1999-12-14 Cold Spring Harbor Laboratory Ubiquitin ligases, and uses related thereto
US6747128B2 (en) 1997-08-20 2004-06-08 Gpc Biotech, Inc. Components of ubiquitin ligase complexes, and uses related thereto
CN1297053A (en) * 1999-11-22 2001-05-30 上海博容基因开发有限公司 Polypeptide-human ubiquitin C-end hydrolase 28 and polynucleotide for coding said polypeptide
AU2001267181A1 (en) * 2000-05-30 2001-12-11 Viridis Biotech Inc. Polyubiquitin based hydrogel and uses thereof
US20050032683A1 (en) 2000-10-04 2005-02-10 Amento Edward P. Methods of modulating apoptosis by administration of relaxin agonists or antagonists
ES2295370T3 (en) 2001-07-06 2008-04-16 Lg Life Sciences Ltd. NEW AMINOPEPTIDASA DERIVED FROM BACILLUS LICHENIFORMIS, GEN CODIFYING THE AMINOPEPTIDASE, VECTOR OF EXPRESSION CONTAINING THE GEN, TRANSFORMANT AND METHOD FOR PREPARATION.
EP1417237B1 (en) * 2001-07-26 2011-04-06 Advanced Protein Technologies Corp. Process for preparation of polypeptides of interest from fusion peolypeptides
CA2467505A1 (en) * 2001-12-12 2003-06-19 Eli Lilly And Company Expression system
MX2007007591A (en) * 2004-12-22 2007-07-25 Ambrx Inc Methods for expression and purification of recombinant human growth hormone.
EP2275431A3 (en) * 2008-01-18 2011-05-25 Bio-Rad Laboratories, Inc. Enhanced fractionation of phosphorylated and nonphosphorylated biomolecules by apatite chromotography

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4757013A (en) * 1983-07-25 1988-07-12 The Research Foundation Of State University Of New York Cloning vehicles for polypeptide expression in microbial hosts
US4703004A (en) * 1984-01-24 1987-10-27 Immunex Corporation Synthesis of protein with an identification peptide
US4771002A (en) * 1984-02-24 1988-09-13 Lubrizol Genetics, Inc. Transcription in plants and bacteria
WO1988002406A2 (en) * 1986-10-02 1988-04-07 Massachusetts Institute Of Technology Methods of regulating metabolic stability of proteins
ATE113985T1 (en) * 1987-03-20 1994-11-15 Creative Biomolecules Inc PROCEDURE FOR PURIFICATION OF RECOMBINANT POLYPEPTIDES.

Also Published As

Publication number Publication date
EP0420936B1 (en) 1994-04-13
AU3865989A (en) 1990-01-12
US5108919A (en) 1992-04-28
FI906214A0 (en) 1990-12-17
JPH03505527A (en) 1991-12-05
DK303790D0 (en) 1990-12-21
DE68914664T2 (en) 1994-10-20
EP0420936A1 (en) 1991-04-10
AU619319B2 (en) 1992-01-23
WO1989012678A1 (en) 1989-12-28
FI906214A7 (en) 1990-12-17
ES2018901A6 (en) 1991-05-16
DK303790A (en) 1991-02-22

Similar Documents

Publication Publication Date Title
DE68914664D1 (en) PURIFIED UBIQUITIN HYDROLASE, THESE ENCODING DNA SEQUENCES, AND THEIR USE TO OBTAIN POLYPEPTIDES.
DE3587864D1 (en) RECOMBINANT DNA MOLECULES.
DE68928368D1 (en) Recombinant DNA, bacterium of the genus Pseudomonas containing it and its use for the production of lipase
DE69009601D1 (en) Exhaust gas purification catalyst.
DE3864585D1 (en) DNA MOLECULES, CODING FOR NON-A-NON-B-HEPATITIS ANTIGENS AND THEIR USE FOR THE PRODUCTION OF THESE ANTIGENS.
FI962247A7 (en) Novel apoptosis-modifying proteins, the DNA encoding them, and methods of using them
EP0571442A4 (en) CYTOKIN INDUCIBLE PROTEIN TSG-14, ITS ENCODING DNA AND THEIR USE.
MX9203598A (en) NOVEL POLYPEPTIDE AND DNA SEQUENCES CODING FOR ERYTHROPOYETIN SYNTHESIS.
FI871124A7 (en) Polypeptide, DNA and its use.
DE3887744D1 (en) New pigments and their use.
DE69411692D1 (en) Means to promote the proliferation of Bifidobacterium
DE69015566D1 (en) Reversible modification of biological compounds to identify, separate and purify them.
FI941048L (en) DNA sequences encoding gelonin polypeptide
DE3576524D1 (en) DNA SEQUENCES.
FI860677A7 (en) Rhinovirus strain HRV2 polypeptide and the DNA molecule encoding it.
DE68901294D1 (en) HYDROGEN TREATMENT CATALYST.
FI863626A7 (en) DNA sequences, recombinant DNA molecules and methods for producing human lipocortin-like polypeptides.
FI873615A7 (en) Rhinovirus strain HRV89 polypeptides and the DNA molecules encoding them
DE3585890D1 (en) PEPTID AND GENERAL CODING GENE.
DE3783145D1 (en) RECOMBINANT DNA PLASMIDE FOR THE PRODUCTION OF XANTHAN GUM.
DE59004374D1 (en) New polymers and their use.
DE69124561D1 (en) METHOD FOR PURIFYING POLYPEPTIDES
FI902803A7 (en) DNA sequence encoding the enzyme α-amylase-pullulanase
ATE104344T1 (en) PURIFIED UBIQUITIN HYDROLASE, THIS ENCODING DNA SEQUENCES AND THEIR USE FOR OBTAINING POLYPEPTIDES.
DE69012109D1 (en) DNA SEQUENCING USING CHEMOLUMINESCENCE.

Legal Events

Date Code Title Description
8364 No opposition during term of opposition