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DE68901191D1 - SELF-DISPLAYING ANALYSIS WITH IMPROVED RESOLUTION USING STOECHIOMETRIC CHEMICAL SUBTRACTION. - Google Patents

SELF-DISPLAYING ANALYSIS WITH IMPROVED RESOLUTION USING STOECHIOMETRIC CHEMICAL SUBTRACTION.

Info

Publication number
DE68901191D1
DE68901191D1 DE8989101762T DE68901191T DE68901191D1 DE 68901191 D1 DE68901191 D1 DE 68901191D1 DE 8989101762 T DE8989101762 T DE 8989101762T DE 68901191 T DE68901191 T DE 68901191T DE 68901191 D1 DE68901191 D1 DE 68901191D1
Authority
DE
Germany
Prior art keywords
analyte
reaction
indicator
response
self
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE8989101762T
Other languages
German (de)
Inventor
Joel M Blatt
Steven C Charlton
Bert Walter
Mary Ellen Warchal
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bayer Corp
Original Assignee
Miles Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Miles Inc filed Critical Miles Inc
Application granted granted Critical
Publication of DE68901191D1 publication Critical patent/DE68901191D1/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/008—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions for determining co-enzymes or co-factors, e.g. NAD, ATP
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/54—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving glucose or galactose

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Emergency Medicine (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Analysing Materials By The Use Of Radiation (AREA)
  • Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)

Abstract

An analytical method and test composition for determining whether an analyte is present in a test sample at a predetermined concentration level by measuring or observing a preselected level of indicator response, e.g., color generation. A subtractive reaction is carried out on the test sample prior to the indicator reaction in order to stoichiometrically and specifically consume a predetermined amount of analyte. The resulting indicator reaction generates the preselected level of indicator response only if the analyte is present at the predetermined concentration level or greater. The subtractive reaction is enzymatically specific for the analyte in the reaction mixture and the amount of analyte consumed can be closely controlled by the amount of a regulating coreactant present at initiation. The method enables the determination of analyte without the need for standards or comparators, e.g., color charts, and therefore is self-indicating. In another embodiment, there is provided a method and test composition for improving an otherwise suboptimal assay response due to oversaturation of the response, e.g., color, generated by an indicator reaction.
DE8989101762T 1988-02-10 1989-02-02 SELF-DISPLAYING ANALYSIS WITH IMPROVED RESOLUTION USING STOECHIOMETRIC CHEMICAL SUBTRACTION. Expired - Fee Related DE68901191D1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US15422588A 1988-02-10 1988-02-10

Publications (1)

Publication Number Publication Date
DE68901191D1 true DE68901191D1 (en) 1992-05-21

Family

ID=22550517

Family Applications (1)

Application Number Title Priority Date Filing Date
DE8989101762T Expired - Fee Related DE68901191D1 (en) 1988-02-10 1989-02-02 SELF-DISPLAYING ANALYSIS WITH IMPROVED RESOLUTION USING STOECHIOMETRIC CHEMICAL SUBTRACTION.

Country Status (8)

Country Link
EP (1) EP0327952B1 (en)
JP (1) JP2767049B2 (en)
AT (1) ATE74969T1 (en)
AU (1) AU602277B2 (en)
CA (1) CA1322324C (en)
DE (1) DE68901191D1 (en)
DK (1) DK59589A (en)
ES (1) ES2035377T3 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2037562A1 (en) * 1990-06-18 1991-12-19 Robert P. Hatch Detection assays having chromogenic and nonchromogenic, hypochromogenic, bathochromogenic or hypsochromogenic substrates
GB9308411D0 (en) * 1993-04-23 1993-06-09 Celsis Ltd Detection of biological material
EP1173745A4 (en) * 1999-02-05 2006-04-12 Taylor Technologies Inc Multicomponent test systems useful in analyzing liquid samples, and uses therefor
DK1504113T3 (en) * 2002-05-16 2007-03-05 Hoffmann La Roche Process for preparing polymer layers
WO2011136063A1 (en) * 2010-04-30 2011-11-03 日東紡績株式会社 Method for measurement of specific substance, and kit for measurement of specific substance
WO2020047606A1 (en) * 2018-09-06 2020-03-12 The University Of Sydney Systems, sensors and methods for determining a concentration of an analyte

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BE563751A (en) * 1957-01-08
AT347600B (en) * 1974-11-11 1979-01-10 Wellcome Found TEST EQUIPMENT
US3964871A (en) * 1974-12-18 1976-06-22 Becton, Dickinson And Company Method and device for detecting glucose
US4654310A (en) * 1984-01-10 1987-03-31 Ly Uy Vu Instrumentless quantitative analysis system
CA1295216C (en) * 1986-04-04 1992-02-04 James P. Albarella Rainbow test device
US4898813A (en) * 1986-04-04 1990-02-06 Albarella James P Catalytic test composition intended to produce a range of colors

Also Published As

Publication number Publication date
EP0327952B1 (en) 1992-04-15
DK59589A (en) 1989-08-11
JP2767049B2 (en) 1998-06-18
ES2035377T3 (en) 1993-04-16
AU2680788A (en) 1989-08-10
DK59589D0 (en) 1989-02-09
CA1322324C (en) 1993-09-21
EP0327952A1 (en) 1989-08-16
ATE74969T1 (en) 1992-05-15
JPH0231697A (en) 1990-02-01
AU602277B2 (en) 1990-10-04

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee