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DE618789C - Process for obtaining physiologically active substances from the anterior pituitary gland - Google Patents

Process for obtaining physiologically active substances from the anterior pituitary gland

Info

Publication number
DE618789C
DE618789C DEI35149D DEI0035149D DE618789C DE 618789 C DE618789 C DE 618789C DE I35149 D DEI35149 D DE I35149D DE I0035149 D DEI0035149 D DE I0035149D DE 618789 C DE618789 C DE 618789C
Authority
DE
Germany
Prior art keywords
active substances
anterior pituitary
pituitary gland
physiologically active
obtaining physiologically
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
DEI35149D
Other languages
German (de)
Inventor
Dr Carl Ludwig Lautenschlaeger
Dr Fritz Lindner
Dr Willy Ludwig
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
IG Farbenindustrie AG
Original Assignee
IG Farbenindustrie AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by IG Farbenindustrie AG filed Critical IG Farbenindustrie AG
Priority to DEI35149D priority Critical patent/DE618789C/en
Application granted granted Critical
Publication of DE618789C publication Critical patent/DE618789C/en
Expired legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • A61K38/22—Hormones
    • A61K38/24—Follicle-stimulating hormone [FSH]; Chorionic gonadotropins, e.g. HCG; Luteinising hormone [LH]; Thyroid-stimulating hormone [TSH]

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Endocrinology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Reproductive Health (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Zoology (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Description

Verfahren zur Gewinnung physiologisch wirksamer Stoffe aus Hypophysenvorderlappen In. dem Patent 614861 ist ein Verfahren zur Gewinnung von physiologisch wirksamen Stoffen aus Hypophysenvorderlappen beschrieben, welches darin besteht, daß die Drüsen einer kombinierten Behandlung mit organischen und wässerrigen Lösungsmitteln unterwoifen werden.Process for the extraction of physiologically active substances from the anterior pituitary gland In. the patent 614861 is a method for the production of physiologically active Substances from the anterior pituitary gland described, which consists in the glands subject to a combined treatment with organic and aqueous solvents will.

Brei weiterer Bearbeitung des Verfahrens wurde nun gefunden, daß man zu günstigeren Resultaten, namentlich bezüglich der Stärke der wässerigen Extrakte, gelangen kann, wenn man die Drüsen nach erfolgter Behandlung mit organischen Lösungsmitteln bei der anschließenden Extraktion mit wässerigen Lösungsmitteln gleichzeitig einem Autolyse- bzw. Verdauungsprozeß und gegebenenfalls die erhaltenen wässerigen. Extrakte einer Nachbehandlung nach dem Verfahren des Hauptpatents unterwirft. Die letztere Maßnahme ist besonders angezeigt, um bei der Autolyse oder Verdauung evtl. entstandene Hemmungskörper zu entfernen.Much further processing of the process has now been found that one to more favorable results, especially with regard to the strength of the aqueous extracts, can get if the glands after treatment with organic solvents in the subsequent extraction with aqueous solvents simultaneously one Autolysis or digestion process and optionally the aqueous ones obtained. Extracts subject to post-treatment according to the procedure of the main patent. The latter Measure is particularly indicated to avoid any resulting during autolysis or digestion Remove the inhibitor.

Die wässerigen Extrakte können dann durch Weiterbehandlung mit wasserunlöslichen Lösungsmitteln von Ballaststoffen, wie Lipoiden usw., befreit werden. Beispiele i. io g vorbehandelte Hypophysenvorderlappen werden mit i oö ccm Wasser unter Zugabe eines Konservierun.gsmitt@els bei 2o bis 25° C mehrere Tage autolysiert, das Ganze zweimal mit je der doppelten Menge Aceton versetzt rund das. Gesamtextrakt im Vakuum von Aceton befreit. Der wässerige Anteil wird mit Äther ausgeschüttelt und nach Entfernung des Äthers am Tier ausgewertet.The aqueous extracts can then be treated with water-insoluble Solvents of dietary fiber, such as lipoids, etc., can be freed. Examples i. 10 g pretreated anterior pituitary lobes are mixed with 100 cc of water with the addition of a Conservierun.gsmitt@els autolyzed at 2o to 25 ° C for several days, the whole thing twice with twice the amount of acetone added around the total extract in vacuo freed from acetone. The watery part is shaken out with ether and after Evaluation of the removal of the ether on the animal.

2. 5 g Trockenpräparat werden mit o,5 g Papain (i :8o) und 5o ccm Wasser mit oder ohne Pufferlösung unter Zusatz eines Konservierungsmittels (nach Einleitung der Verdauung bei erhöhter Temperatur) mehrere Tage bei 20° C verdaut. Durch scharfes Zentrifugieren erhält man eine klare Lösung mit geringem Eiweißgehalt, die am Tier ausgewertet rund gegebenenfalls mach Beispiel i weiter gereinigt werden kann.2. 5 g dry preparation are mixed with 0.5 g papain (i: 8o) and 50 ccm Water with or without a buffer solution with the addition of a preservative (according to Initiation of digestion at elevated temperature) digested for several days at 20 ° C. Sharp centrifugation gives a clear solution with a low protein content, which are evaluated on the animal and possibly further cleaned according to example i can.

Claims (1)

PATENTAPTSPRUCIi: Abänderung des Verfahrens zur Gewinnung physiologisch wirksamer Stoffe aus Hypophysenvorderlappen nach Patent 6rq.86r, dadurch gekennzeichnet, daß man die Drüsen . nach erfolgter Behandlung mit organischen- Lösungsmitteln bei an-.schließender Ertraktion mit wässerigen Lösungsmitteln gleichzeitig einem Autolyse- bzw. Verdauungsprozeß unterwirft ,und gegebenenfalls die erhaltenen wässerigen Extrakteeiner Nachbehandlung nach dem Verfahren des Hauptpatents unterzieht.PATENTAPTSPRUCIi: Modification of the method of obtaining physiological active substances from the anterior pituitary gland according to patent 6rq.86r, characterized in that one the glands. after treatment with organic Solvents with subsequent extraction with aqueous solvents at the same time subjected to an autolysis or digestion process, and optionally the obtained subjecting aqueous extracts to post-treatment according to the method of the main patent.
DEI35149D 1928-08-05 1928-08-05 Process for obtaining physiologically active substances from the anterior pituitary gland Expired DE618789C (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
DEI35149D DE618789C (en) 1928-08-05 1928-08-05 Process for obtaining physiologically active substances from the anterior pituitary gland

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DEI35149D DE618789C (en) 1928-08-05 1928-08-05 Process for obtaining physiologically active substances from the anterior pituitary gland

Publications (1)

Publication Number Publication Date
DE618789C true DE618789C (en) 1935-09-14

Family

ID=7188905

Family Applications (1)

Application Number Title Priority Date Filing Date
DEI35149D Expired DE618789C (en) 1928-08-05 1928-08-05 Process for obtaining physiologically active substances from the anterior pituitary gland

Country Status (1)

Country Link
DE (1) DE618789C (en)

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