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DE60333673D1 - Überwachungsprotein zur messung der proteinverarbeitung - Google Patents

Überwachungsprotein zur messung der proteinverarbeitung

Info

Publication number
DE60333673D1
DE60333673D1 DE60333673T DE60333673T DE60333673D1 DE 60333673 D1 DE60333673 D1 DE 60333673D1 DE 60333673 T DE60333673 T DE 60333673T DE 60333673 T DE60333673 T DE 60333673T DE 60333673 D1 DE60333673 D1 DE 60333673D1
Authority
DE
Germany
Prior art keywords
protein
monitor
measuring
processing
protein processing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60333673T
Other languages
English (en)
Inventor
Yoshihiro Ohmiya
Emiko Ashitaka
Seiji Ito
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Japan Science and Technology Agency
Original Assignee
Japan Science and Technology Agency
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Japan Science and Technology Agency filed Critical Japan Science and Technology Agency
Application granted granted Critical
Publication of DE60333673D1 publication Critical patent/DE60333673D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62DNA sequences coding for fusion proteins
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/01Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/09Fusion polypeptide containing a localisation/targetting motif containing a nuclear localisation signal
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/60Fusion polypeptide containing spectroscopic/fluorescent detection, e.g. green fluorescent protein [GFP]
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00Screening for compounds of potential therapeutic value

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Hematology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
DE60333673T 2002-12-12 2003-12-11 Überwachungsprotein zur messung der proteinverarbeitung Expired - Lifetime DE60333673D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2002360744 2002-12-12
PCT/JP2003/015828 WO2004052934A1 (ja) 2002-12-12 2003-12-11 蛋白質のプロセッシングを測定するためのモニター蛋白質

Publications (1)

Publication Number Publication Date
DE60333673D1 true DE60333673D1 (de) 2010-09-16

Family

ID=32501001

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60333673T Expired - Lifetime DE60333673D1 (de) 2002-12-12 2003-12-11 Überwachungsprotein zur messung der proteinverarbeitung

Country Status (7)

Country Link
US (1) US20070224647A2 (de)
EP (1) EP1580196B1 (de)
JP (1) JP4427671B2 (de)
CN (1) CN1726228B (de)
AU (1) AU2003289316A1 (de)
DE (1) DE60333673D1 (de)
WO (1) WO2004052934A1 (de)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7989621B2 (en) 2005-09-26 2011-08-02 National Institute Of Advanced Industrial Science And Technology Method for producing substituted imidazo[1,2-A]pyrazines of (s)-1-(3-(2-sec-butyl-6-(1h-indol-3-yl)-3-oxo-3,7-dihydroimadazo[1,2-a]pyrazin-8-yl)propyl)guanidine
CN101410459B (zh) 2006-03-30 2013-03-27 旭化成化学株式会社 树脂组合物及其成型品
US8147842B2 (en) 2006-06-12 2012-04-03 National Institute Of Advanced Industrial Science And Technology Mutant luciferase
WO2012124799A1 (en) 2011-03-15 2012-09-20 Olympus Corporation Star-worm luciferase
JP5997131B2 (ja) 2011-03-16 2016-09-28 国立大学法人東北大学 生体組織分析用プローブ及びその利用法
JP6868232B2 (ja) * 2015-03-20 2021-05-12 国立大学法人東京工業大学 コラーゲン融合タンパク質、及びそれを用いた薬剤のスクリーニング方法
CN115011621A (zh) * 2022-06-27 2022-09-06 齐鲁工业大学 一种重组蛋白及荧光检测底物的方法

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6228604B1 (en) * 1996-04-10 2001-05-08 Loma Linda University Secreted renilla luciferase
CA2335305C (en) * 1998-06-16 2006-05-23 Biosignal Packard Inc. A bioluminescence resonance energy transfer (bret) system and its use
AU2001247445B2 (en) * 2000-03-15 2006-09-21 Gaussia L.L.C. Renilla reniformis fluorescent proteins, nucleic acids encoding the fluorescent proteins and the use thereof in diagnostics, high throughput screening and novelty items
WO2002025284A2 (en) * 2000-09-25 2002-03-28 U.S. Medical Research Institute Of Infectious Diseases High-throughput assays for the proteolytic activities of clostridial neurotoxins
JPWO2004022600A1 (ja) * 2002-09-06 2005-12-22 独立行政法人産業技術総合研究所 分泌型又は膜結合型キメラ蛋白質

Also Published As

Publication number Publication date
EP1580196A1 (de) 2005-09-28
EP1580196A4 (de) 2006-06-21
EP1580196B1 (de) 2010-08-04
AU2003289316A1 (en) 2004-06-30
US20060035286A1 (en) 2006-02-16
CN1726228B (zh) 2010-05-26
AU2003289316A8 (en) 2004-06-30
JPWO2004052934A1 (ja) 2006-04-13
WO2004052934A1 (ja) 2004-06-24
CN1726228A (zh) 2006-01-25
US20070224647A2 (en) 2007-09-27
JP4427671B2 (ja) 2010-03-10

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