DE60310847T2 - Use of an anti-tumor agent - Google Patents

Description

Background of the invention

1. Field of the invention

The The present invention relates to an antitumor agent having an anti-tumor effect.

2. Description of the related technology

In Japan it is in use since the antiquity, at the rhizome of Consume bamboo growing bamboo shoots. Further, the use is of powdered completely mature bamboo known as cattle feed.

The Abstract of JP-A-2002017248 describes an ethanol-water extract of Phyllostachys pubescens. The extract can be used to maintain the Fresh used by food.

The Abstract of JP-A-2001095521 also describes a bamboo extract, which by cutting the bamboo into pieces and soaking this in an aqueous ethyl alcohol made with at least 35% ethanol. The extract can be used as a seasoning be used.

The Abstract from Journal of the Korean Society of Food Science and Nutrition, 1996, Issue 25, No. 5, pages 741-746 describes an extract from bamboo leaves, wherein the extract was obtained by placing the bamboo leaves in extracted from a 70% ethanol solution were. According to the abstract the corresponding extract has an antibacterial effect.

Summary the invention

The Applicant has found that a Alcohol extract of bamboo, which may include bamboo shoots, has an excellent antitumour effect. The present invention is based on this realization, and a task of this is to enter To provide antitumor agents having an antitumor effect.

The previously mentioned Task is solved by the use of an extract for the preparation of an anti-tumor agent, wherein the extract with an alcohol solution of Moso bamboo (Phyllostachys pubescens) is extracted. This object is further solved by the use of an extract for the preparation of an antitumor agent, wherein the extract with a alcohol solution is extracted from bamboo, the bamboo being the sprout meat of Moso bamboo (Phyllostachys pubescens) or the outer part a fully grown Tribe of Moso bamboo is. Alcohol extracts of bamboo have one excellent antitumor effect; if therefore a health food with This alcohol extract of bamboo can be regularly consumed orally the growth of malignant Tumors (cancer) are prevented or limited. The antitumor effect of such an alcohol extract is in an aqueous Alcohol solution which contains water, stronger than with a 100% alcohol solution. The alcohol concentration of such an aqueous alcohol solution is preferably 50 to 70%, and it is particularly preferred that the alcohol concentration this aqueous alcohol solution 60% is. Relative to the bamboo is the sprouts of Moso bamboo (Phyllostachys pubescens) and the outer part of the strain of the same Kind of bamboo particularly preferred.

Further are antitumor agents with the aforementioned extract as an active Component thereof extremely effective in the treatment of malignant tumors.

Summary the drawings

1 is a graph showing the results (right leg) for study 1;

2 is a graph showing the results (left leg) for Examination 1;

3 Figure 3 is a graph showing the results for study 2;

4 Figure 3 is a graph showing the results for study 2;

5 Figure 3 is a graph showing the results for study 2;

6 Fig. 12 is a graph showing the results for Examination 3;

7 Fig. 12 is a graph showing the results for Examination 3;

8th Fig. 12 is a graph showing the results for Examination 3; and

9 is a graph showing the results for study 4.

description the preferred embodiments

in the Following are exemplary embodiments of the present invention. First, it should be noted that the as the primary one Material serving bamboo is Moso bamboo. Both fully mature Bamboo as well as bamboo shoots can be used. The parts of the bamboo plant that uses can be include the trunk, roots and leaves. It is preferred that the bamboo is fresh, and that others Plant parts as the sprouts within one year of harvest be used. If bamboo shoots are used, it is preferable that these be processed shortly after harvest. In any case, however, will the bamboo is frozen while he is still fresh, so he is looking for a use for a later Time can be stored.

Subsequently, will the bamboo washed to remove impurities, and then in a mill ground to a particle size, which fits through a 10 mm wire screen. For mills that do not use wire mesh equipped are, the bamboo can be ground to a particle length between 3 and 5 mm become. Subsequently is 1 kg of ground bamboo in 10 kg of an alcohol solution or an aqueous alcohol solution dipped until completely is bleached. The amount of time it takes is usually approximately 6 months. If, however, at about Heated to 60 ° C, last about 2 hours.

Subsequently, will separated the solid component in a centrifuge, and the liquid bamboo alcohol extract is won back. The liquid Extract is caused by a warming to 60 ° C to 20% of the original Concentrated weight. If an equivalent amount of solid starch to this Given concentrated bamboo extract, this can be used to form a powder, the powder being the Antitumor agent acts. It is a matter of course that this Powder vitamins and the like added can be. The antitumor agent may take the form of a solid, a powder, a liquid, one Granules or the like exhibit.

at the process described above the slag remaining after centrifuging is recovered become. Six parts by weight of an alcohol solution or an aqueous alcohol solution can too each one part by weight of the slag and the liquid, by heating for 2 hours at 60 ° C can be obtained with the liquid which are obtained in the above-described process has been. In this way, the bamboo can be completely exploited without loss become.

If the antitumor agent described above as an injectable agent is to be used, a resin cleaning process is further carried out, and although prior to concentration to 20% of the original volume, as with the process above has been described, and the concentrated extract is ethanol or the like further deproteinized. This is then subjected to microfiltration, to cleanse it, and bacteria will be removed, and subsequently will it for example for 30 minutes at 120 ° C autoclaved. In ethanol deproteinization, the product becomes by heating to 80 ° C concentrated to completely remove the alcohol.

As From the above description, the present invention includes Invention the technical idea of a "method for producing an anti-tumor agent, the method comprising a step of bamboo in an alcohol solution or an aqueous one alcohol solution is immersed, and a step in which a liquid alcohol extract this bamboo is regained and concentrated ".

rehearse a product according to the invention, which according to the continue Methods described above were used in the following Investigations 1 to 4 tested for antitumor activity.

Examination 1

Malignant sacro-cells (Meth-A fibrosarcoma) were transferred to both subcutaneous groin areas of BALB / c mice, a tumor was grown, and changes in the surface area of the tumor over time were measured for 25 days. For 3 days, starting on the third day after the transfer, 0.1 ml of a sample A (a liquid in which a powder is dissolved in a physiological saline solution, the powder of Moso bamboo using an aqueous alcohol solution with a Alcohol concentration of 60% was extracted) into the interior of the tumor on the right leg of each mouse. Under the same conditions, a physiological saline solution was administered to a control group. The content of powder in 0.1 ml of Sample A was 1 mg. The results are in the graph of 1 and 2 shown.

As based on the in 1 and 2 As shown in the results of the study, tumor growth was suppressed by administration of sample A in both legs. Changes in the surface area of the tumor in the right leg represent the direct effect of Sample A, while the changes in the surface area of the tumor in the left leg are believed to be the result of sensitization and activation of immune functions in the organism as a response Sample A are.

Examination 2

Ascites tumor cells (sarcoma-180) in the amount of 1 x 10 ⁶ were transferred to the right subcutaneous inguinal region of ICR mice to form massive tumors. A sample B for which a powder is dissolved in a physiological saline solution wherein the powder of Moso bamboo is extracted with an aqueous ethanol solution having an ethanol concentration of 60%, and a sample C, wherein the sample is a powder in a physiological Saline solution, the Moso bamboo powder being extracted with a 100% ethanol solution, was orally administered, starting at 24 hours post-transplant, at 1000 mg / kg / day. Administration was performed on each subsequent day and the mice were bred for 28 days. Under the same conditions, a control group was given a physiological saline solution, and a bamboo extract obtained with hot water was used as a control. Meanwhile, the surface area of the tumors and the change in body mass of the ICR mice were measured, and on the 28th day, the tumors were removed and weighed. Changes in the surface areas of the tumors are in the graph of 3 Changes in the weight of the tumors are shown in the graph of 4 shown and changes in body mass are in the graph of 5 shown.

As it is based on the in 3 As can be seen from the results shown, the values at both sample B and sample C are significantly lower than those of the control group, from day 12 to day 28. Compared with this, the values for the hot water-derived bamboo extract are only significantly lower than those of the bamboo extract Control group on day 26, but a significant difference was not found on day 28. Further, as in the graph of 4 The values for the weights of the removed tumors were significantly lower in Example B and C than in the control group, but a significant difference was not found between the control group and the group with the bamboo extract obtained with hot water. These findings make it clear that samples B and C inhibit tumor growth without affecting body growth, demonstrating excellent antitumor activity. As it is from the graph of 5 During the study period, there was no significant difference in body growth between groups and no sample had any effect on the growth of the mice.

Examination 3

• ^* p < 0,01 verglichen mit Kontrollgruppe

Ascites tumor cells (Sakrom-180) in the amount of 1.2 x 10 ⁶ were transferred to the right subcutaneous groin of ICR mice and grown to form massive tumors. Starting 24 hours after the transplantation, a sample D for which a powder was dissolved in a physiological saline solution was extracted from bamboo shoots using a 60% ethanol aqueous solution and a sample E prepared in the same manner bamboo mixture (a mixture of approximately equal amounts of Japanese wooden bamboo, Moso bamboo, black bamboo (Phyllostachys nigra var. nigera), arrow bamboo (Pseudosasa japonica), Hachiku bamboo, Medake bamboo (Pleioblatus simonii), all of which growing in Japan, and Indonesian tali bamboo), and a sample F, which is made in the same way from Indonesian tali bamboo was given orally, at a dose of 1000 mg / kg / day. The administration was performed every other day, and from each group, 9 mice were bred for 28 days. A physiological saline solution was administered to a control group under the same conditions. During this time, the surface areas of the tumors were measured over time. Tumors were removed on day 28 and weighed. The results are shown in Table 1 and in the graphs of 6 to 8th shown. In Table 1, the tumor inhibition rate (%) is represented by the formula [l- (weight of tumors of the group to which the product of the present invention was administered / weight of the tumors in the control group)] × 100 based on the weight of the tumors on the latter Day. Table 1 Study of antitumor activity on ascitic tumor cells

• ^* p <0.01 compared to control group

Out This investigation 3 was recognized that while the strength was dependent different from the bamboo species, all the samples D to F, compared with the control groups, excellent Have anti-tumor effects.

Examination 4

Moso bamboo (Sample G), the outer part completely full-grown trunk of Moso bamboo (sample H), the inner part completely full-grown trunk of Moso bamboo (Sample I) (note: the outer section the trunk is a thin one Area that about the half of the trunk, and the inner part is a thick section, the approximately the half of the tribe), the meat of the bamboo shoots, which the inner part of the shoot is, with the skin removed, and being this the part that is usually used for cooking (sample J), and the skin of bamboo shoots (sample K) were in aqueous ethanol solutions immersed with ethanol concentrations of 60%, which for two hours on Heated to 60 ° C, whereupon the liquid Extract was filtered, concentrated in vacuo (60 ° C) and dried was (60 ° C). It should be noted that for each a portion of sample H 10 parts of ethanol solution were used, and for each a portion of the other samples were used 5 parts ethanol solution.

Sarcoma 180 cells at 1 × 10 ⁷ cells / ml were transplanted into the right abdomen of ICR mice (male, five weeks old) and normal diet at day 0 post-transplantation (Nosan Corporation, Labo MR Breeder ^TM ) of the control group while allowing the test group to take free from the feed to which samples D to K were admixed in a ratio of 20%. Each group included 10 animals. On the 28th day after transplant, the tumors were removed and weighed. The average weights of the tumors are in 9 shown.

From study 4 it was recognized that the meat of bamboo shoots (sample J) was significantly effective samer is considered the skin of bamboo shoots (Sample K). It was also recognized that in the same type of bamboo, the outer part of the fully grown strain (Sample H) was significantly more effective than the inner part of the fully grown strain (Sample I). Moso bamboo has been found to be highly effective.

For the alcohol solution was Ethanol but methanol and other alcohol solutions may be used become. Further, in this embodiment, an aqueous alcohol solution with However, an alcohol concentration of 60% can be used for the Mass production an aqueous one alcohol solution be used with an alcohol concentration between 50 and 70%.

Claims (4)

Using an extract made from Moso bamboo (Phyllostachys pubescens) was extracted with an alcoholic solution for preparation an antitumor agent. Use of an extract made of bamboo with a alcohol solution was extracted, with the bamboo sprouts or the outer part one completely full-grown Moso bamboo strain of Moso bamboo (Phyllostachys pubescens), for the production of an antitumor agent. The use of an extract according to claim 1 or 2, wherein the extract with an aqueous alcohol solution with an alcohol concentration of 50 to 70% is extracted. The use of an extract according to a the claims 1 or 2, wherein the extract with an aqueous alcohol solution with is extracted from bamboo at an alcohol concentration of 60%.