DE60038029D1 - Methoden und reagenzien zur in situ amplifizierung - Google Patents
Methoden und reagenzien zur in situ amplifizierungInfo
- Publication number
- DE60038029D1 DE60038029D1 DE60038029T DE60038029T DE60038029D1 DE 60038029 D1 DE60038029 D1 DE 60038029D1 DE 60038029 T DE60038029 T DE 60038029T DE 60038029 T DE60038029 T DE 60038029T DE 60038029 D1 DE60038029 D1 DE 60038029D1
- Authority
- DE
- Germany
- Prior art keywords
- methods
- reagents
- amplification
- situ
- situ amplification
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 230000003321 amplification Effects 0.000 title abstract 4
- 238000000034 method Methods 0.000 title abstract 4
- 238000003199 nucleic acid amplification method Methods 0.000 title abstract 4
- 239000003153 chemical reaction reagent Substances 0.000 title 1
- 238000001514 detection method Methods 0.000 abstract 2
- 238000011065 in-situ storage Methods 0.000 abstract 2
- 150000007523 nucleic acids Chemical class 0.000 abstract 2
- 102000039446 nucleic acids Human genes 0.000 abstract 2
- 108020004707 nucleic acids Proteins 0.000 abstract 2
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6865—Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6841—In situ hybridisation
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Steroid Compounds (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US387455 | 1999-09-01 | ||
| US09/387,455 US6187567B1 (en) | 1999-09-01 | 1999-09-01 | Methods and reagents for in situ amplification |
| PCT/US2000/023734 WO2001016374A2 (en) | 1999-09-01 | 2000-08-30 | Methods and reagents for in situ amplification |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE60038029D1 true DE60038029D1 (de) | 2008-03-27 |
| DE60038029T2 DE60038029T2 (de) | 2009-02-05 |
Family
ID=23529941
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE60038029T Expired - Lifetime DE60038029T2 (de) | 1999-09-01 | 2000-08-30 | Methoden und reagenzien zur in situ amplifizierung |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US6187567B1 (de) |
| EP (1) | EP1212460B1 (de) |
| JP (1) | JP4608162B2 (de) |
| AT (1) | ATE386140T1 (de) |
| DE (1) | DE60038029T2 (de) |
| WO (1) | WO2001016374A2 (de) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20040146918A1 (en) * | 2000-02-18 | 2004-07-29 | Weiner Michael L. | Hybrid nucleic acid assembly |
| WO2004068104A2 (en) * | 2003-01-24 | 2004-08-12 | The Government Of The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services | Target activated microtransfer |
| US7695752B2 (en) | 2003-01-24 | 2010-04-13 | The Government Of The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services | Target activated microtransfer |
| US7957466B2 (en) * | 2005-09-16 | 2011-06-07 | Sony Corporation | Adaptive area of influence filter for moving object boundaries |
| US20080299622A1 (en) * | 2007-02-07 | 2008-12-04 | Paulson Bradley A | Starch Hydrolysis Using Phytase with an Alpha Amylase |
| US20110147837A1 (en) * | 2009-12-23 | 2011-06-23 | Hafez Walid M | Dual work function gate structures |
Family Cites Families (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4886741A (en) | 1987-12-09 | 1989-12-12 | Microprobe Corporation | Use of volume exclusion agents for the enhancement of in situ hybridization |
| US5168038A (en) | 1988-06-17 | 1992-12-01 | The Board Of Trustees Of The Leland Stanford Junior University | In situ transcription in cells and tissues |
| US5021335A (en) | 1988-06-17 | 1991-06-04 | The Board Of Trustees Of The Leland Stanford Junior University | In situ transcription in cells and tissues |
| US5225326A (en) | 1988-08-31 | 1993-07-06 | Research Development Foundation | One step in situ hybridization assay |
| AU645915B2 (en) | 1991-07-23 | 1994-01-27 | F. Hoffmann-La Roche Ag | Improvements in the in situ PCR |
| US5506105A (en) | 1991-12-10 | 1996-04-09 | Dade International Inc. | In situ assay of amplified intracellular mRNA targets |
| US5589333A (en) | 1992-02-03 | 1996-12-31 | Thomas Jefferson University | In situ polymerase chain reaction |
| US5521061A (en) | 1992-07-17 | 1996-05-28 | Aprogenex, Inc. | Enhancement of probe signal in nucleic acid-mediated in-situ hybridization studies |
| US5863726A (en) * | 1993-11-12 | 1999-01-26 | Geron Corporation | Telomerase activity assays |
| GB2293238A (en) * | 1994-09-13 | 1996-03-20 | Inceltec Ltd | Primers for replication and/or amplification reactions |
| GB9516636D0 (en) * | 1995-08-14 | 1995-10-18 | Univ London | In-situ nucleic acid amplification and detection |
| EP0796347A2 (de) * | 1995-09-21 | 1997-09-24 | Becton, Dickinson and Company | Nachweis von nukleinsäure in zelle unter verwendung von thermophilen strangverdrängungsamplifikation |
-
1999
- 1999-09-01 US US09/387,455 patent/US6187567B1/en not_active Expired - Lifetime
-
2000
- 2000-08-30 WO PCT/US2000/023734 patent/WO2001016374A2/en active IP Right Grant
- 2000-08-30 AT AT00957907T patent/ATE386140T1/de not_active IP Right Cessation
- 2000-08-30 JP JP2001520919A patent/JP4608162B2/ja not_active Expired - Fee Related
- 2000-08-30 EP EP00957907A patent/EP1212460B1/de not_active Expired - Lifetime
- 2000-08-30 DE DE60038029T patent/DE60038029T2/de not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| US6187567B1 (en) | 2001-02-13 |
| EP1212460B1 (de) | 2008-02-13 |
| DE60038029T2 (de) | 2009-02-05 |
| JP2003519471A (ja) | 2003-06-24 |
| WO2001016374A2 (en) | 2001-03-08 |
| EP1212460A2 (de) | 2002-06-12 |
| WO2001016374A3 (en) | 2001-11-01 |
| ATE386140T1 (de) | 2008-03-15 |
| JP4608162B2 (ja) | 2011-01-05 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8364 | No opposition during term of opposition |