DE3719177A1 - Material for a bionic cornea - Google Patents
Material for a bionic corneaInfo
- Publication number
- DE3719177A1 DE3719177A1 DE19873719177 DE3719177A DE3719177A1 DE 3719177 A1 DE3719177 A1 DE 3719177A1 DE 19873719177 DE19873719177 DE 19873719177 DE 3719177 A DE3719177 A DE 3719177A DE 3719177 A1 DE3719177 A1 DE 3719177A1
- Authority
- DE
- Germany
- Prior art keywords
- bionic
- corneal endothelial
- endothelial cells
- cornea
- proteins
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 239000000463 material Substances 0.000 title claims abstract description 30
- 239000011664 nicotinic acid Substances 0.000 title claims abstract description 9
- 210000004087 cornea Anatomy 0.000 title claims abstract description 8
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 8
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 8
- 125000000837 carbohydrate group Chemical group 0.000 claims abstract description 7
- 150000001720 carbohydrates Chemical class 0.000 claims description 5
- 210000004027 cell Anatomy 0.000 claims description 5
- 235000014633 carbohydrates Nutrition 0.000 claims description 4
- 239000000203 mixture Substances 0.000 claims description 4
- 239000000126 substance Substances 0.000 claims description 4
- 150000001413 amino acids Chemical class 0.000 claims description 2
- 150000002500 ions Chemical class 0.000 claims description 2
- 235000015097 nutrients Nutrition 0.000 claims description 2
- 235000016709 nutrition Nutrition 0.000 claims description 2
- 230000035764 nutrition Effects 0.000 claims description 2
- 102000004196 processed proteins & peptides Human genes 0.000 claims description 2
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 2
- 238000001356 surgical procedure Methods 0.000 claims description 2
- 210000002889 endothelial cell Anatomy 0.000 claims 1
- 210000000399 corneal endothelial cell Anatomy 0.000 abstract description 10
- 239000007858 starting material Substances 0.000 abstract description 5
- 230000002093 peripheral effect Effects 0.000 abstract description 2
- 230000001464 adherent effect Effects 0.000 abstract 1
- 230000021164 cell adhesion Effects 0.000 abstract 1
- 230000004663 cell proliferation Effects 0.000 abstract 1
- 230000002062 proliferating effect Effects 0.000 abstract 1
- 239000013543 active substance Substances 0.000 description 5
- 229920006362 Teflon® Polymers 0.000 description 4
- 230000004048 modification Effects 0.000 description 4
- 238000012986 modification Methods 0.000 description 4
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 2
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 229920002678 cellulose Polymers 0.000 description 2
- 239000001913 cellulose Substances 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 230000002209 hydrophobic effect Effects 0.000 description 2
- 238000002513 implantation Methods 0.000 description 2
- 208000006069 Corneal Opacity Diseases 0.000 description 1
- SHZGCJCMOBCMKK-UHFFFAOYSA-N D-mannomethylose Natural products CC1OC(O)C(O)C(O)C1O SHZGCJCMOBCMKK-UHFFFAOYSA-N 0.000 description 1
- WQZGKKKJIJFFOK-QTVWNMPRSA-N D-mannopyranose Chemical compound OC[C@H]1OC(O)[C@@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-QTVWNMPRSA-N 0.000 description 1
- 102000016359 Fibronectins Human genes 0.000 description 1
- 108010067306 Fibronectins Proteins 0.000 description 1
- PNNNRSAQSRJVSB-SLPGGIOYSA-N Fucose Natural products C[C@H](O)[C@@H](O)[C@H](O)[C@H](O)C=O PNNNRSAQSRJVSB-SLPGGIOYSA-N 0.000 description 1
- 229920002683 Glycosaminoglycan Polymers 0.000 description 1
- 102000005744 Glycoside Hydrolases Human genes 0.000 description 1
- 108010031186 Glycoside Hydrolases Proteins 0.000 description 1
- 108700023372 Glycosyltransferases Proteins 0.000 description 1
- SHZGCJCMOBCMKK-DHVFOXMCSA-N L-fucopyranose Chemical compound C[C@@H]1OC(O)[C@@H](O)[C@H](O)[C@@H]1O SHZGCJCMOBCMKK-DHVFOXMCSA-N 0.000 description 1
- 101710133652 Lectin-like protein Proteins 0.000 description 1
- 102000004895 Lipoproteins Human genes 0.000 description 1
- 108090001030 Lipoproteins Proteins 0.000 description 1
- OVRNDRQMDRJTHS-CBQIKETKSA-N N-Acetyl-D-Galactosamine Chemical compound CC(=O)N[C@H]1[C@@H](O)O[C@H](CO)[C@H](O)[C@@H]1O OVRNDRQMDRJTHS-CBQIKETKSA-N 0.000 description 1
- MBLBDJOUHNCFQT-UHFFFAOYSA-N N-acetyl-D-galactosamine Natural products CC(=O)NC(C=O)C(O)C(O)C(O)CO MBLBDJOUHNCFQT-UHFFFAOYSA-N 0.000 description 1
- 239000004743 Polypropylene Substances 0.000 description 1
- 239000004793 Polystyrene Substances 0.000 description 1
- 208000012287 Prolapse Diseases 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- WQZGKKKJIJFFOK-PHYPRBDBSA-N alpha-D-galactose Chemical compound OC[C@H]1O[C@H](O)[C@H](O)[C@@H](O)[C@H]1O WQZGKKKJIJFFOK-PHYPRBDBSA-N 0.000 description 1
- 230000024245 cell differentiation Effects 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 230000008602 contraction Effects 0.000 description 1
- 231100000269 corneal opacity Toxicity 0.000 description 1
- 238000005530 etching Methods 0.000 description 1
- 125000000524 functional group Chemical group 0.000 description 1
- 229930182830 galactose Natural products 0.000 description 1
- 235000011187 glycerol Nutrition 0.000 description 1
- 102000045442 glycosyltransferase activity proteins Human genes 0.000 description 1
- 108700014210 glycosyltransferase activity proteins Proteins 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- 230000000642 iatrogenic effect Effects 0.000 description 1
- 239000007943 implant Substances 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 229920001684 low density polyethylene Polymers 0.000 description 1
- 239000004702 low-density polyethylene Substances 0.000 description 1
- 229940041290 mannose Drugs 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000001404 mediated effect Effects 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 229920005615 natural polymer Polymers 0.000 description 1
- 239000012454 non-polar solvent Substances 0.000 description 1
- 229920000515 polycarbonate Polymers 0.000 description 1
- 239000004417 polycarbonate Substances 0.000 description 1
- -1 polypropylene Polymers 0.000 description 1
- 229920001155 polypropylene Polymers 0.000 description 1
- 229920002223 polystyrene Polymers 0.000 description 1
- 230000001172 regenerating effect Effects 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 239000011877 solvent mixture Substances 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 229920001059 synthetic polymer Polymers 0.000 description 1
- 230000008733 trauma Effects 0.000 description 1
- 230000002792 vascular Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
- A61F2/00—Filters implantable into blood vessels; Prostheses, i.e. artificial substitutes or replacements for parts of the body; Appliances for connecting them with the body; Devices providing patency to, or preventing collapsing of, tubular structures of the body, e.g. stents
- A61F2/02—Prostheses implantable into the body
- A61F2/14—Eye parts, e.g. lenses or corneal implants; Artificial eyes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
- A61F2/00—Filters implantable into blood vessels; Prostheses, i.e. artificial substitutes or replacements for parts of the body; Appliances for connecting them with the body; Devices providing patency to, or preventing collapsing of, tubular structures of the body, e.g. stents
- A61F2/02—Prostheses implantable into the body
- A61F2/14—Eye parts, e.g. lenses or corneal implants; Artificial eyes
- A61F2/142—Cornea, e.g. artificial corneae, keratoprostheses or corneal implants for repair of defective corneal tissue
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/54—Biologically active materials, e.g. therapeutic substances
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
- A61L2300/21—Acids
- A61L2300/214—Amino acids
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
- A61L2300/23—Carbohydrates
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
- A61L2300/25—Peptides having up to 20 amino acids in a defined sequence
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
- A61L2300/252—Polypeptides, proteins, e.g. glycoproteins, lipoproteins, cytokines
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Transplantation (AREA)
- Public Health (AREA)
- Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Oral & Maxillofacial Surgery (AREA)
- Veterinary Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Dermatology (AREA)
- Epidemiology (AREA)
- Ophthalmology & Optometry (AREA)
- Cardiology (AREA)
- Heart & Thoracic Surgery (AREA)
- Vascular Medicine (AREA)
- Molecular Biology (AREA)
- Materials For Medical Uses (AREA)
Abstract
Description
Die Erfindung betrifft ein Material, welches Anwendung in der operativen Augenheilkunde findet. Ausgangspunkt ist die Tatsache, daß Hornhauttransplantationen, welche nach Traumata, z.B. Ver ätzungen bzw. bei Hornhauttrübungen notwendig sind, durch den Mangel an humanen Spenderhornhäuten limitiert sind.The invention relates to a material which application in the operative ophthalmology takes place. Starting point is the fact that corneal transplants, which after trauma, e.g. Ver etching or corneal opacity are necessary due to the Lack of human donor corneas are limited.
Aufgabe der Erfindung ist die Schaffung eines Materials für eine bionische Hornhaut. Der Begriff bionisch (eine Zusammenziehung aus den Begriffen biologisch und technisch) resultiert daher, daß als technischer Anteil des erfindungsgemäßen Materials es sich zunächst um bekannte Materialien handelt, die aber erst nach einer biologischen Oberflächenmodifikation bestimmungsgemäß als Ersatz für die bisher verwendeten humanen Spenderhornhäute ver wendet werden können.The object of the invention is to create a material for a bionic cornea. The term bionic (a contraction from the terms biological and technical) it follows that as a technical part of the material according to the invention initially known materials, but only after a biological surface modification as intended Replacement for the previously used human donor corneas ver can be applied.
Dies bedeutet, daß nach einer Keratotomie, die unter Belassung eines peripheren Corneaendothelzellrings durchgeführt wurde, der iatrogen gesetzte Defekt mit diesem bionischen Material gedeckt wird, wo dann im weiteren Verlauf, die verbliebenen Corneaendo thelzellen des Patienten auf der Materialoberfläche fest haftend, diese geordnet bedeckend wachsen. Das bionische Material dient also als Unterlage (Substrat, "support") für regenerationsfähige, metabolisch aktive Corneaendothelzellen. This means that after a keratotomy that is left under of a peripheral corneal endothelial cell ring, which iatrogenic defect covered with this bionic material where, in the further course, the remaining Corneaendo patient's cell cells firmly adhering to the material surface, these grow orderly covering. The bionic material serves So as a base (substrate, "support") for regenerative, metabolically active corneal endothelial cells.
Der technische Anteil des bionischen Materials besteht darin, daß das Ausgangsmaterial folgende Eigenschaften aufweisen muß: es muß durchsichtig, UV-beständig, zur Ernährung für die Corneaendothel zellen für Ionen und Nährstoffe permeabel, flexibel, immunologisch inert, nähbar und im µm-Bereich dünn ist. Diese Materialien sind als sysnthetische oder natürliche Polymere bekannt. Als Beispiele seien niedermolekulares Polystyrol, Polyäthylen und Polypropylen niederer Dichte, Teflon (R), Polycarbonate etc., ebenso wie Materialien aus (chemisch modifizierter) Cellulose oder auch (vernetztem) Protein genannt.The technical part of the bionic material is that the starting material must have the following properties: it must be transparent, UV-resistant, permeable to ions and nutrients for nutrition for the corneal endothelial cells, flexible, immunologically inert, sewable and thin in the µm range . These materials are known as synthetic or natural polymers. Examples include low molecular weight polystyrene, low density polyethylene and polypropylene, Teflon ( R ), polycarbonates etc., as well as materials made from (chemically modified) cellulose or (crosslinked) protein.
Die erfindungsgemäße biologische Oberflächenmodifikation o.g. Materialien mit biologisch aktiven Substanzen hat zum Ziel, daß, wenn nach Operation dieses bionische Material den iatrogen ge setzten Defekt deckt, dieses Material eine Unterlage darstellt, auf der die verbliebenen Corneaendothelzellen fest haftend und sich vermehrend geordnet wachsen.The biological surface modification according to the invention mentioned above. The aim of materials with biologically active substances is that if after surgery this bionic material passes the iatrogen covers defect, this material is a base, on which the remaining corneal endothelial cells adhere firmly and grow in orderly order.
Hierzu ist es notwendig, daß die unmittelbare Materialoberfläche Kohlenhydratgruppenketten und/oder Proteine aufweist, die für die Corneaendothelzellen "Ankermöglichkeiten" darstellen, (siehe auch H. Rauvala, Trends in Biochemistry Science, 7, 323-325 (1983), woraus hervorgeht, daß Interaktionen von lektinähnlichen Prote inen, Glykosidasen und Glykosyltransferasen auf der Zellmembran oberfläche mit bestimmten Kohlenhydratgruppenketten und/oder Proteinen Interaktionen eingehen, die Voraussetzung für Zell adhärenz und Zelldifferenzierung sind).For this it is necessary that the immediate surface of the material Has carbohydrate group chains and / or proteins for the Represent corneal endothelial cells "anchor options" (see also H. Rauvala, Trends in Biochemistry Science, 7, 323-325 (1983), which indicates that interactions of lectin-like protein in, glycosidases and glycosyltransferases on the cell membrane surface with certain carbohydrate group chains and / or Proteins interact, the prerequisite for cells adherence and cell differentiation are).
Derartige Kohlenhydratgruppenketten, die zur erfindungsgemäßen biologischen Oberflächenmodifikation eingsetzt werden, finden sich beispielsweise als die polaren Molekülanteile von Glycerin phosphatiden, Spingolip(o)iden, Glykosphingolip(o)iden oder Lipo proteinen und sind beispielsweise als Lip(o)id-Extrakt leicht erhältlich und umfassen je nach Aufarbeitung und Ausgangsmaterial unterschiedliche Mengen an (acetylierten und/oder aminierten) verzweigten oder unverzweigten Kohlenhydraten wie Fucose, Man nose, Galaktose, sowie N-Acetylgalactosamin, N-Acylneuraminsäure als Reinsubstanzen oder Mischungen. Weiterhin werden Heteropoly saccharide, Glykosaminglykanen eingesetzt, die bekanntermaßen für die fibronectin-vermittelte Zelladhärenz auf Oberflächen ver antwortlich sind. - Es versteht sich, daß der prozentuale Anteil der einzelnen bei der biologischen Oberflächenmodifikation eingesetzten biologisch aktiven Substanzen, also der einzelnen Kohlenhydrate bzw. Kohlenhydratgruppenketten und/oder Proteine, Peptide bzw. auch Aminosäuren, jeweils frei variierbar ist und den in vivo Erfordernissen optimal angepasst wird.Such carbohydrate group chains for the invention biological surface modification are used for example as the polar moiety of glycerin phosphatiden, Spingolip (o) iden, Glykosphingolip (o) iden or Lipo proteins and are light, for example, as lip (o) id extract available and include depending on the processing and starting material different amounts of (acetylated and / or aminated) branched or unbranched carbohydrates such as fucose, Man nose, galactose, and N-acetylgalactosamine, N-acylneuraminic acid as pure substances or mixtures. Furthermore, heteropoly saccharide, glycosaminoglycans, which are known for the fibronectin-mediated cell adherence on surfaces ver are answerable. - It is understood that the percentage of the individual in the biological surface modification used biologically active substances, i.e. the individual Carbohydrates or carbohydrate group chains and / or proteins, Peptides or amino acids, each is freely variable and is optimally adapted to the in vivo requirements.
Die o.g. biologisch aktiven Substanzen werden auf die Material oberflächen entweder adsorptiv oder kovalent gebunden. Verfahren hierzu sind bekannt: durch Lösen von amphiphilen Substanzen, wie sie die oben erwähnten Lip(o)ide darstellen, in einem im wesent lichen unpolaren Lösungsmittel (gemisch) und anschließendem in innigen-Kontakt-bringen mit einem Material, welches die auf Seite 2 beschriebenen Voraussetzungen erfüllt, beispielsweise Teflon (R), und danach völligem Entfernen des Lösungsmittel (gemisches), lagern sich die amphiphilen Substanzen mit ihrem hydrophoben Molekülanteil über van der Waals′sche und hydrophobe Kräfte fest an die Materialoberfläche an, wobei der hydrophile aus Kohlen hydratgruppenketten und/oder Proteinen bestehende Molekülanteil nunmehr die unmittelbare Materialoberfläche darstellt und die oben erwähnten "Ankermöglichkeiten" für die Corenaendothelzellen bietet.The above biologically active substances are either adsorptively or covalently bound to the material surfaces. Methods for this are known: by dissolving amphiphilic substances, such as those represented by the lip (o) ide mentioned above, in an essentially non-polar solvent (mixture) and then bringing them into intimate contact with a material which corresponds to that on page 2 fulfills the described conditions, for example Teflon ( R ), and then completely remove the solvent (mixture), the amphiphilic substances with their hydrophobic molecular component are firmly attached to the surface of the material via van der Waals'sche and hydrophobic forces, the hydrophilic group consisting of carbohydrates and / or proteins present in the molecule now represents the immediate surface of the material and offers the above-mentioned "anchor options" for the corenaendothelial cells.
Die kovalente Bindung der biologisch aktiven Substanzen auf die Materialoberfläche setzt voraus, daß die Oberfläche des Ausgangs materials genügend reaktive, funktionelle Gruppen, z.B. Hydroxi gruppen bei der Verwendung des Cellulosederivats Cellophan (R), aufweist. Diese Gruppen könnten, falls erforderlich, beispiels weise durch CnBr, noch aktiviert werden, so daß eine stabile kovalente Bindung zwischen Materialoberfläche und den einge setzten biologisch aktiven Substanzen entsteht.The covalent bonding of the biologically active substances to the material surface presupposes that the surface of the starting material has sufficient reactive, functional groups, for example hydroxyl groups, when using the cellulose derivative Cellophan ( R ). These groups could, if necessary, be activated by CnBr, for example, so that a stable covalent bond between the material surface and the biologically active substances used is formed.
Die derart biologisch oberflächenmodifizierten Materialien sind leicht und somit preiswert herstellbar, sie können auf den be nötigten Durchmesser zurecht geschnitten werden, da sie nach erfolgter Keratotomie sofort eingenäht werden, schützen sie nach Implantation sofort den hinteren Teil des Auges, eine Gefäßproli feration, wie sie bei der Implantation von Spenderhornhäuten häufig eintritt, wird wegen der unmittelbar nach Implantation noch fehlenden Corneaendothelzellschicht nicht eintreten, dafür bietet dieses Material aber den vorhandenen Corneaendothelzellen die Möglichkeit, sich fest haftend und geordnet auf dieser bio nischen Materialoberfläche anzusiedeln.The biologically surface-modified materials are easy and therefore inexpensive to manufacture, you can on the be required diameter can be cut to size as they are If the keratotomy is sewn in immediately, protect it afterwards Immediately implant the posterior part of the eye, a vascular prolapse feration, as in the implantation of donor corneas occurs frequently because of immediately after implantation do not enter the missing corneal endothelial cell layer, instead but offers this material to the existing corneal endothelial cells the opportunity to stick firmly and orderly on this bio niche material surface.
Als Ausgangsmaterial wird 5 µm starkes, durchsichtiges Teflon (R) eingesetzt. Dieses Material wird kurz in einen Chloroform/Me thanol (2 : 1 v/v) Gesamtlip(o)id-Extrakt aus EDTA-Vollblut des Patienten getaucht und wieder herausgezogen (9 Teile Lösungs mittelgemisch : 1 Teil EDTA-Blut). Das derart lip(o)id beschichtete Teflon (R)-Material wird getrocknet, dann gründlich mit Ringerlösung gewaschen und in Ringerlösung dampfsterilisert und kann danach implantiert werden.5 µm thick, transparent Teflon ( R ) is used as the starting material. This material is briefly immersed in a chloroform / methanol (2: 1 v / v) total lip (o) id extract from the patient's EDTA whole blood and withdrawn again (9 parts of solvent mixture: 1 part of EDTA blood). The Teflon ( R ) material coated with lip (o) id in this way is dried, then washed thoroughly with Ringer's solution and steam-sterilized in Ringer's solution and can then be implanted.
Claims (1)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19873719177 DE3719177A1 (en) | 1987-06-09 | 1987-06-09 | Material for a bionic cornea |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19873719177 DE3719177A1 (en) | 1987-06-09 | 1987-06-09 | Material for a bionic cornea |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE3719177A1 true DE3719177A1 (en) | 1988-12-29 |
Family
ID=6329309
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE19873719177 Withdrawn DE3719177A1 (en) | 1987-06-09 | 1987-06-09 | Material for a bionic cornea |
Country Status (1)
| Country | Link |
|---|---|
| DE (1) | DE3719177A1 (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3939648A1 (en) * | 1989-11-30 | 1991-06-06 | Adatomed Pharma & Med | Intra=ocular lens of silicone rubber - with surface coated with protein with affinity to inner side of natural lens casing |
| WO2005037144A2 (en) * | 2003-10-10 | 2005-04-28 | Cellular Bioengineering, Inc. | Methods and compositions for growing corneal endothelial and related cells on biopolymers and creation of artifical corneal transplants |
-
1987
- 1987-06-09 DE DE19873719177 patent/DE3719177A1/en not_active Withdrawn
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3939648A1 (en) * | 1989-11-30 | 1991-06-06 | Adatomed Pharma & Med | Intra=ocular lens of silicone rubber - with surface coated with protein with affinity to inner side of natural lens casing |
| WO2005037144A2 (en) * | 2003-10-10 | 2005-04-28 | Cellular Bioengineering, Inc. | Methods and compositions for growing corneal endothelial and related cells on biopolymers and creation of artifical corneal transplants |
| WO2005037144A3 (en) * | 2003-10-10 | 2005-07-14 | Ge Ming Liu | Methods and compositions for growing corneal endothelial and related cells on biopolymers and creation of artifical corneal transplants |
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