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DE3688163D1 - Verfahren zur rueckgewinnung von extrazellulaeren enzymen aus gesamten fermentationsbruehen. - Google Patents

Verfahren zur rueckgewinnung von extrazellulaeren enzymen aus gesamten fermentationsbruehen.

Info

Publication number
DE3688163D1
DE3688163D1 DE8686111604T DE3688163T DE3688163D1 DE 3688163 D1 DE3688163 D1 DE 3688163D1 DE 8686111604 T DE8686111604 T DE 8686111604T DE 3688163 T DE3688163 T DE 3688163T DE 3688163 D1 DE3688163 D1 DE 3688163D1
Authority
DE
Germany
Prior art keywords
recovery
extracellular enzymes
entire fermentation
brews
fermentation brews
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE8686111604T
Other languages
English (en)
Other versions
DE3688163T2 (de
Inventor
Jack W Brewer
Charles E Brothers
Terry F Farver
Chong Y Kim
Eunkyu Kyu Lee
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Danisco US Inc
Original Assignee
Solvay Enzymes Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Solvay Enzymes Inc filed Critical Solvay Enzymes Inc
Application granted granted Critical
Publication of DE3688163D1 publication Critical patent/DE3688163D1/de
Publication of DE3688163T2 publication Critical patent/DE3688163T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/814—Enzyme separation or purification
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/814—Enzyme separation or purification
    • Y10S435/816—Enzyme separation or purification by solubility

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
DE86111604T 1985-09-04 1986-08-22 Verfahren zur Rückgewinnung von extrazellulären Enzymen aus gesamten Fermentationsbrühen. Expired - Fee Related DE3688163T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US77503185A 1985-09-04 1985-09-04
US06/890,620 US4728613A (en) 1985-09-04 1986-08-05 Method for the recovery of extracellular enzymes from whole fermentation beer

Publications (2)

Publication Number Publication Date
DE3688163D1 true DE3688163D1 (de) 1993-05-06
DE3688163T2 DE3688163T2 (de) 1993-09-30

Family

ID=27118985

Family Applications (1)

Application Number Title Priority Date Filing Date
DE86111604T Expired - Fee Related DE3688163T2 (de) 1985-09-04 1986-08-22 Verfahren zur Rückgewinnung von extrazellulären Enzymen aus gesamten Fermentationsbrühen.

Country Status (7)

Country Link
US (1) US4728613A (de)
EP (1) EP0214531B1 (de)
CN (1) CN1014799B (de)
DE (1) DE3688163T2 (de)
DK (1) DK421086A (de)
FI (1) FI93655C (de)
MX (1) MX167654B (de)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0262651A3 (de) * 1986-09-30 1990-06-20 Union Carbide Corporation Isolierung von Enzymen aus einer Lösung
WO1989005863A1 (en) * 1987-12-23 1989-06-29 Gist-Brocades N.V. Purified industrial enzyme and process for the preparation thereof
DE3908422A1 (de) * 1989-03-15 1990-09-20 Forschungszentrum Juelich Gmbh Verfahren zur extraktion von proteinen aus waessrigen biomassesuspensionen
US5139943A (en) * 1989-06-13 1992-08-18 Genencor International, Inc. Processes for the recovery of microbially produced chymosin
US5151358A (en) * 1989-06-13 1992-09-29 Genencor International, Inc. Processes for the recovery of naturally produced chymosin
WO1990015864A1 (en) * 1989-06-13 1990-12-27 Genencor International, Inc. Processes for the recovery of microbially produced chymosin
CA2093422C (en) * 1990-10-05 2001-04-03 DETERGENT COMPOSITIONS CONTAINING LOW CBH I CONTENT CELLULASE COMPOSITIONS
US5290474A (en) * 1990-10-05 1994-03-01 Genencor International, Inc. Detergent composition for treating cotton-containing fabrics containing a surfactant and a cellulase composition containing endolucanase III from trichoderma ssp
US5328841A (en) * 1990-10-05 1994-07-12 Genencor International, Inc. Methods for isolating EG III cellulase component and EG III cellulase in polyethylene glycol using inorganic salt and polyethylene glycol
SE9003534D0 (sv) * 1990-11-06 1990-11-06 Kabigen Ab A method for isolating and purifying peptides and proteins
US5281526A (en) * 1992-10-20 1994-01-25 Solvay Enzymes, Inc. Method of purification of amylase by precipitation with a metal halide and 4-hydroxybenzic acid or a derivative thereof
JPH10512758A (ja) * 1995-01-27 1998-12-08 ジェネンコア インターナショナル インコーポレーテッド 界面活性剤に基づく抽出法
DE10024437A1 (de) * 2000-05-19 2001-11-29 Aventis Res & Tech Gmbh & Co Verfahren zur selektiven Herstellung von Essigsäure durch katalytische Oxidation von Ethan
ES2252287T3 (es) 2000-07-28 2006-05-16 Henkel Kommanditgesellschaft Auf Aktien Enzima amilolitico de bacillus sp. a7-7 (dsm 12368) asi com0 agentes de lavado y de limpieza con este nuevo enzima amilolitico.
FR2827192B1 (fr) * 2001-07-13 2004-06-04 Cognis France Sa Preparations contenant des agents tensio-actifs non ioniques comme agents d'extraction
EP1485495B1 (de) * 2002-03-15 2010-09-15 Iogen Energy Corporation Verfahren zur herstellung von glukose durch verwendung von endoglucanase-core-protein zur verbesserten rückgewinnung und wiederverwendung des enzyms
ATE420161T1 (de) * 2002-07-01 2009-01-15 Novozymes As Monopropylenglykol als fermentationszusatz
GB0218021D0 (en) * 2002-08-05 2002-09-11 Ciba Spec Chem Water Treat Ltd Production of a fermentation product
GB0402470D0 (en) 2004-02-04 2004-03-10 Ciba Spec Chem Water Treat Ltd Production of a fermentation product
GB0402469D0 (en) * 2004-02-04 2004-03-10 Ciba Spec Chem Water Treat Ltd Production of a fermentation product
EP1761552B1 (de) 2004-06-29 2008-09-17 Ares Trading S.A. Verfahren zur aufreinigung von il-18-bindendem protein
DE102008013490A1 (de) * 2008-03-10 2009-09-17 Bayer Technology Services Gmbh Verfahren zur Reinigung therapeutischer Proteine
US20120122183A1 (en) * 2009-07-09 2012-05-17 Novozymes A/S Flocculation with divalent salt and phosphate
CN101893528B (zh) * 2010-08-04 2011-12-21 江南大学 一种制备绍兴黄酒成品麦曲中微生物胞外酶双向电泳样品的方法
EP2831215B1 (de) * 2012-03-29 2018-08-08 Novozymes A/S Verwendung von enzymen zur herstellung von wasserlöslichen folien

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR596444A (fr) * 1925-04-07 1925-10-23 Kalle & Co Ag Procédé pour la séparation des enzymes de leurs solutions
NL286954A (de) * 1962-01-03
CA940070A (en) * 1968-12-23 1974-01-15 Jim S. Berry Stabilized aqueous enzyme composition
DE2512735A1 (de) * 1975-03-22 1976-09-30 Henkel & Cie Gmbh Verfahren zur gewinnung von proteinen aus waessrigen protein- loesungen
GB1589581A (en) * 1976-04-14 1981-05-13 Biotechnolog Forschung Gmbh Process for the separation of enzymes
DE2639129C3 (de) * 1976-08-31 1979-10-04 Gesellschaft Fuer Biotechnologische Forschung Mbh (Gbf), 3300 Braunschweig Verfahren zur Abtrennung von Enzymen
JPS5739796A (en) * 1980-08-21 1982-03-05 Unitika Ltd Separation of nucleic acid and protein in ground cell solution
JPS5815989A (ja) * 1981-06-12 1983-01-29 Sanki Eng Kk 生理活性蛋白質の精製方法
US4508825A (en) * 1983-03-30 1985-04-02 Miles Laboratories, Inc. Method for the separation of extracellular amylase and protease produced during the fermentation of enzyme producing microorganisms
FR2545501B1 (fr) * 1983-05-06 1985-08-02 Elf Bio Rech Procede de purification de la dextrane-saccharase

Also Published As

Publication number Publication date
FI93655B (fi) 1995-01-31
CN1014799B (zh) 1991-11-20
DK421086A (da) 1987-03-05
US4728613A (en) 1988-03-01
DE3688163T2 (de) 1993-09-30
FI93655C (fi) 1995-05-10
CN86106017A (zh) 1987-04-08
DK421086D0 (da) 1986-09-03
EP0214531A2 (de) 1987-03-18
FI863540A0 (fi) 1986-09-02
MX167654B (es) 1993-04-01
EP0214531B1 (de) 1993-03-31
EP0214531A3 (en) 1988-05-25
FI863540L (fi) 1987-03-05

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8327 Change in the person/name/address of the patent owner

Owner name: GENENCOR INTERNATIONAL, INC. (N.D.GES.D. STAATES D

8339 Ceased/non-payment of the annual fee