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DE3676604D1 - Expression von epa in saeugetierzellen. - Google Patents

Expression von epa in saeugetierzellen.

Info

Publication number
DE3676604D1
DE3676604D1 DE8686902215T DE3676604T DE3676604D1 DE 3676604 D1 DE3676604 D1 DE 3676604D1 DE 8686902215 T DE8686902215 T DE 8686902215T DE 3676604 T DE3676604 T DE 3676604T DE 3676604 D1 DE3676604 D1 DE 3676604D1
Authority
DE
Germany
Prior art keywords
epa
expression
mammal cells
mammal
cells
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE8686902215T
Other languages
English (en)
Inventor
Nancy Haigwood
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novartis Vaccines and Diagnostics Inc
Original Assignee
Chiron Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Chiron Corp filed Critical Chiron Corp
Application granted granted Critical
Publication of DE3676604D1 publication Critical patent/DE3676604D1/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/48Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424Serine endopeptidases (3.4.21)
    • C12N9/6456Plasminogen activators
    • C12N9/6459Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y304/00Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21Serine endopeptidases (3.4.21)
    • C12Y304/21069Protein C activated (3.4.21.69)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00Nucleic acids vectors
    • C12N2800/10Plasmid DNA
    • C12N2800/108Plasmid DNA episomal vectors

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
DE8686902215T 1985-03-22 1986-03-20 Expression von epa in saeugetierzellen. Expired - Fee Related DE3676604D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US71523785A 1985-03-22 1985-03-22
PCT/US1986/000577 WO1986005514A1 (en) 1985-03-22 1986-03-20 EXPRESSION OF tPA IN MAMMALIAN CELLS

Publications (1)

Publication Number Publication Date
DE3676604D1 true DE3676604D1 (de) 1991-02-07

Family

ID=24873205

Family Applications (1)

Application Number Title Priority Date Filing Date
DE8686902215T Expired - Fee Related DE3676604D1 (de) 1985-03-22 1986-03-20 Expression von epa in saeugetierzellen.

Country Status (4)

Country Link
EP (1) EP0215923B1 (de)
JP (2) JPH0740942B2 (de)
DE (1) DE3676604D1 (de)
WO (1) WO1986005514A1 (de)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2581668B2 (ja) * 1985-11-27 1997-02-12 三井東圧化学株式会社 ヒト正常細胞由来のヒト組織プラスミノ−ゲン活性化因子をコ−ドする新しいdna配列とそれを含むベクタ−及び細胞
DE3545126A1 (de) * 1985-12-19 1987-06-25 Boehringer Mannheim Gmbh Verfahren zur verbesserung der expression von eukaryontenprotein
IL82746A (en) * 1986-06-06 1994-10-07 Genentech Inc Process for producing biologically active tissue-type plasminogen activator
AU1187588A (en) * 1987-01-15 1988-08-10 Codon Genetic Engineering Laboratories Tandem gene eukaryotic expression vectors
US5024939A (en) * 1987-07-09 1991-06-18 Genentech, Inc. Transient expression system for producing recombinant protein
US4929560A (en) * 1988-02-03 1990-05-29 Damon Biotech, Inc. Recovery of tissue plasminogen activator
WO1989010959A1 (en) * 1988-05-06 1989-11-16 Codon Supertransformants for high expression rates in eukaryotic cells
FR2642767B1 (fr) * 1989-01-19 1993-10-01 Transgene Sa Vecteurs d'expression de proteines heterologues dans les cellules eucaryotes, lignees cellulaires obtenues et procede pour leur preparation

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0173552B1 (de) * 1984-08-24 1991-10-09 The Upjohn Company Rekombinante DNA-Verbindungen und Expression von Polypeptiden wie tPA
AU593264B2 (en) * 1985-07-10 1990-02-08 Kanegafuchi Kagaku Kogyo Kabushiki Kaisha Chromosomal DNA sequence, expression vector for human tissue plasminogen activating factor, cultured cells transfected with same and method of producing said activating factor

Also Published As

Publication number Publication date
JPH07110227B2 (ja) 1995-11-29
JPS62502585A (ja) 1987-10-08
EP0215923B1 (de) 1990-12-27
EP0215923A4 (de) 1987-04-29
JPH0740942B2 (ja) 1995-05-10
EP0215923A1 (de) 1987-04-01
JPH0767632A (ja) 1995-03-14
WO1986005514A1 (en) 1986-09-25

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Legal Events

Date Code Title Description
8328 Change in the person/name/address of the agent

Free format text: STELLRECHT, W., DIPL.-ING. M.SC. GRIESSBACH, D., DIPL.-PHYS. DR.RER.NAT. HAECKER, W., DIPL.-PHYS. BOEHME, U., DIPL.-PHYS. DR.RER.NAT. BECK, J., DIPL.-PHYS.DR.RER.NAT. WOESSNER, G., DIPL.-CHEM. DR.RER.NAT., PAT.-ANWAELTE, 7000 STUTTGART

8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee