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DE3580418D1 - Elektrophoretisches trennverfahren mit freier stroemung und apparat dafuer. - Google Patents

Elektrophoretisches trennverfahren mit freier stroemung und apparat dafuer.

Info

Publication number
DE3580418D1
DE3580418D1 DE8585109302T DE3580418T DE3580418D1 DE 3580418 D1 DE3580418 D1 DE 3580418D1 DE 8585109302 T DE8585109302 T DE 8585109302T DE 3580418 T DE3580418 T DE 3580418T DE 3580418 D1 DE3580418 D1 DE 3580418D1
Authority
DE
Germany
Prior art keywords
separation process
free flow
electrophoretic separation
electrophoretic
free
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE8585109302T
Other languages
English (en)
Inventor
Katsumi Mouri
Kosai Hiratsuka
Shoji Yoshinaga
Toji Nakatsui
Chikao Oda
Kiyoshi Fujiwara
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Hitachi Ltd
Original Assignee
Hitachi Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from JP59158724A external-priority patent/JPH0715454B2/ja
Priority claimed from JP59199582A external-priority patent/JPS6177751A/ja
Application filed by Hitachi Ltd filed Critical Hitachi Ltd
Application granted granted Critical
Publication of DE3580418D1 publication Critical patent/DE3580418D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44769—Continuous electrophoresis, i.e. the sample being continuously introduced, e.g. free flow electrophoresis [FFE]
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D57/00—Separation, other than separation of solids, not fully covered by a single other group or subclass, e.g. B03C
    • B01D57/02—Separation, other than separation of solids, not fully covered by a single other group or subclass, e.g. B03C by electrophoresis

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Engineering & Computer Science (AREA)
  • Environmental & Geological Engineering (AREA)
  • Peptides Or Proteins (AREA)
DE8585109302T 1984-07-31 1985-07-24 Elektrophoretisches trennverfahren mit freier stroemung und apparat dafuer. Expired - Lifetime DE3580418D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP59158724A JPH0715454B2 (ja) 1984-07-31 1984-07-31 荷電物質の分離精製法およびその装置
JP59199582A JPS6177751A (ja) 1984-09-26 1984-09-26 連続電気泳動装置

Publications (1)

Publication Number Publication Date
DE3580418D1 true DE3580418D1 (de) 1990-12-13

Family

ID=26485754

Family Applications (1)

Application Number Title Priority Date Filing Date
DE8585109302T Expired - Lifetime DE3580418D1 (de) 1984-07-31 1985-07-24 Elektrophoretisches trennverfahren mit freier stroemung und apparat dafuer.

Country Status (3)

Country Link
US (2) US4698142A (de)
EP (1) EP0171676B1 (de)
DE (1) DE3580418D1 (de)

Families Citing this family (43)

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US5080770A (en) * 1989-09-11 1992-01-14 Culkin Joseph B Apparatus and method for separating particles
EP0497077B1 (de) * 1991-01-28 1996-07-17 Ciba-Geigy Ag Vorrichtung zur Vorbereitung von Proben insbesondere für Analysezwecke
US5122246A (en) * 1991-06-26 1992-06-16 Schmidt Joseph L Free flow electrophoresis method
US5302264A (en) * 1992-09-02 1994-04-12 Scientronix, Inc. Capillary eletrophoresis method and apparatus
US5411650A (en) * 1993-07-21 1995-05-02 Eastman Kodak Company Captive vortex high agitation device
US5486337A (en) * 1994-02-18 1996-01-23 General Atomics Device for electrostatic manipulation of droplets
US5562812A (en) * 1995-04-03 1996-10-08 The Penn State Research Foundation Free flow electrophoresis device for biomolecule purification and separation in zero and one G
FR2752751B1 (fr) * 1996-09-02 2000-11-17 Home Alexandre Procede de purification par electrophorese des matieres organiques et utilisations des matieres obtenues
DE69830598T2 (de) 1997-01-31 2006-05-18 The Horticulture And Food Research Institute Of New Zealand Limited Optische vorrichtung und methode
US6149867A (en) 1997-12-31 2000-11-21 Xy, Inc. Sheath fluids and collection systems for sex-specific cytometer sorting of sperm
US6071689A (en) 1997-12-31 2000-06-06 Xy, Inc. System for improving yield of sexed embryos in mammals
US6673225B1 (en) * 1998-05-29 2004-01-06 Industrial Research Limited Method and apparatus for concentrating and/or positioning particles or cells
NZ509434A (en) 1998-07-30 2004-03-26 Univ Colorado State Res Found Equine system for non-surgical artificial insemination
AUPP790698A0 (en) * 1998-12-23 1999-01-28 Life Therapeutics Limited Separation of microorganisms
US7208265B1 (en) 1999-11-24 2007-04-24 Xy, Inc. Method of cryopreserving selected sperm cells
US7077942B1 (en) * 1999-12-23 2006-07-18 Gradipore Limited Removal of biological contaminants
US7141152B2 (en) * 2000-03-16 2006-11-28 Le Febre David A Analyte species separation system
EP1294472B1 (de) 2000-04-18 2009-11-18 Gradipore Limited Trennung und behandlung von proben durch elektrophorese
NZ522613A (en) 2000-05-09 2005-08-26 Xy Inc Method and apparatus for sorting X-chromosome bearing and Y-chromosome bearing populations of spermatozoa and particle differentiation
DE10047088C2 (de) * 2000-09-21 2002-10-17 Gerhard Weber Medium für analytische und präparative Elektrophorese
AU2002237689B2 (en) 2000-11-29 2008-01-10 Xy, Llc. System to separate frozen-thawed spermatozoa into X-chromosome bearing and Y-chromosome bearing populations
US7713687B2 (en) 2000-11-29 2010-05-11 Xy, Inc. System to separate frozen-thawed spermatozoa into x-chromosome bearing and y-chromosome bearing populations
DE10063097B4 (de) * 2000-12-18 2007-04-19 Becton, Dickinson And Co. Elektrophoresevorrichtung
DE10063096C1 (de) * 2000-12-18 2002-09-12 Gerhard Weber Elektrophoresevorrichtung, Elektrophoreseverfahren unter Verwendung einer Elektrophoresevorrichtung und Verwendung der Elektrophoresevorrichtung
EP2283724B8 (de) 2002-08-01 2016-03-16 Xy, Llc Heterospermische Insemination als Zugang zur Spermienfunktion
US8486618B2 (en) 2002-08-01 2013-07-16 Xy, Llc Heterogeneous inseminate system
US7855078B2 (en) 2002-08-15 2010-12-21 Xy, Llc High resolution flow cytometer
US7169548B2 (en) 2002-09-13 2007-01-30 Xy, Inc. Sperm cell processing and preservation systems
NZ577678A (en) 2003-03-28 2010-10-29 Inguran Llc Apparatus and methods for providing sex-sorted animal sperm
NZ544103A (en) 2003-05-15 2010-10-29 Xy Llc Efficient haploid cell sorting for flow cytometer systems
US20050130115A1 (en) * 2003-10-31 2005-06-16 Abs Global,Inc. Method for altering the gender ratio of offspring in mammals by manipulation of spermatozoa
PT2151243E (pt) 2004-03-29 2013-01-25 Inguran Llc Suspensões de esperma para separação em populações enriquecidas carregando o cromossoma x ou y
EP2269617B1 (de) 2004-07-22 2016-04-27 Inguran, LLC Spermasuspensionen zur Sortierung in X- oder Y-Chromosomen-tragende angereicherte Populationen
DE102005020134A1 (de) * 2005-04-29 2006-11-02 Becton, Dickinson And Co. Verfahren und Vorrichtung zur Durchführung eines parallelen und simultanen Mehrfachprozesses der trägerfreien isoelektrischen Fokussierung
US7618770B2 (en) 2005-07-29 2009-11-17 Xy, Inc. Methods and apparatus for reducing protein content in sperm cell extenders
US20080110758A1 (en) * 2006-08-29 2008-05-15 Becton, Dickinson And Company Methods and apparatus for carrier-free deflection electrophoresis
US9879222B2 (en) 2007-12-14 2018-01-30 Mofa Group Llc Gender-specific separation of sperm cells and embryos
CN104350372B (zh) * 2012-08-09 2018-03-02 斯坦福大学托管董事会 用于制备供显微镜分析的生物样本的方法和组合物
CN107058075B (zh) * 2017-06-20 2023-10-20 商丘师范学院 一种植物细胞原生质体纯化仪及纯化方法
CN107245093A (zh) * 2017-08-09 2017-10-13 赖兵 一种蛋白质等电纯化装置及方法
WO2019043656A1 (en) 2017-09-01 2019-03-07 Genus Plc METHODS AND SYSTEMS FOR ASSESSING AND / OR QUANTIFYING POPULATIONS OF SPERMATOZOIDS WITH SEXUAL ASYMMETRY
CN112740024A (zh) * 2018-09-24 2021-04-30 惠普发展公司，有限责任合伙企业 从全血进行粒子分离
KR20240046650A (ko) 2020-09-14 2024-04-09 엔퀴스트 테크놀로지스 아이엔씨. 생물학적 생성물의 제조 및 다운스트림 정제를 위한 공정 기술

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2878178A (en) * 1955-04-15 1959-03-17 Bier Milan Continuous free-boundary flow electrophoresis
DE1442414B2 (de) * 1963-11-18 1971-02-11 Graßmann, Wolfgang, Prof. Dr., 8000 München Vorrichtung zur Durchfuhrung der trägerfreien, kontinuierlichen Elektrophore se in vertikaler Arbeitsweise
US3989613A (en) * 1973-05-16 1976-11-02 The Dow Chemical Company Continuous balanced flow fixed boundary electrophoresis
US4092229A (en) * 1975-12-17 1978-05-30 Bhattacharya Bhairab C Thermal convection counter streaming sedimentation and forced convection galvanization method for controlling the sex of mammalian offspring
US4172774A (en) * 1975-10-30 1979-10-30 Clearwater Systems Inc. Method and apparatus for lessening ionic diffusion
SE415805B (sv) * 1977-06-01 1980-10-27 Gradient Birgit Och Harry Rilb Forfarande for rening av amfolyter medelst isoelektrisk fokusering samt anordning for utforande av forfarandet
US4394246A (en) * 1982-05-24 1983-07-19 Mcdonnell Douglas Corporation Electrophoresis apparatus with flow control
US4465583A (en) * 1982-07-07 1984-08-14 United Kingdom Atomic Energy Authority Electrophoretic separator

Also Published As

Publication number Publication date
US4698142A (en) 1987-10-06
US4749458A (en) 1988-06-07
EP0171676B1 (de) 1990-11-07
EP0171676A2 (de) 1986-02-19
EP0171676A3 (en) 1987-09-23

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee